Restriction map for pF1KSDA0250
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspI        
AvaI        AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BsaAI       
BsaBI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       Bst1107I    BstBI       
BstXI       BstZ17I     ClaI        CpoI        DraI        
DraIII      EaeI        EagI        EclXI       Eco47III    
Eco52I      EcoRV       FbaI        FseI        FspI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MamI        MluI        MluNI       
MroI        MscI        NarI        NciI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         2124  GTCTAC               GT^[AC][GT]AC
AccII          15  CGCG                 CG^CG
AhdI         1121  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2272  CCTCAGG              CC^T[ACGT]AGG
AseI         1473  ATTAAT               AT^TAAT
AsnI         1473  ATTAAT               AT^TAAT
Asp700I      1191  GAATAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1121  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        2629  GAGCTC               G[AT]GC[AT]^C
BamHI        2577  GGATCC               G^GATCC
BglII        2503  AGATCT               A^GATCT
BsaWI        2665  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      2629  GAGCTC               G[AT]GC[AT]^C
BsmI         2591  GAATGC               GAATGC(1/-1)
Bsp1407I      328  TGTACA               T^GTACA
BspMI        2139  GCAGGT               ACCTGC(4/8)
BsrFI        2187  GCCGGC               [GA]^CCGG[CT]
BsrGI         328  TGTACA               T^GTACA
BssHII         14  GCGCGC               G^CGCGC
BssSI        1658  CACGAG               CACGAG(-5/-1)
BstUI          15  CGCG                 CG^CG
Bsu36I       2272  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       2187  GCCGGC               [GA]^CCGG[CT]
Eam1105I     1121  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2272  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2464  GAATTC               G^AATTC
EcoT22I      3184  ATGCAT               ATGCA^T
HaeII         409  AGCGCC               [GA]GCGC^[CT]
HgaI          399  GACGC                GACGC(5/10)
HindIII       860  AAGCTT               A^AGCTT
MaeII        3052  ACGT                 A^CGT
MfeI          373  CAATTG               C^AATTG
MunI          373  CAATTG               C^AATTG
MvnI           15  CGCG                 CG^CG
NaeI         2187  GCCGGC               GCC^GGC
NdeI         1865  CATATG               CA^TATG
NgoMIV       2187  GCCGGC               G^CCGGC
NsiI         3184  ATGCAT               ATGCA^T
NspI          528  GCATGC               [GA]CATG^[CT]
PflMI        1100  CCATGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1473  ATTAAT               AT^TAAT
SacI         2629  GAGCTC               GAGCT^C
SexAI        1979  ACCAGGT              A^CC[AT]GGT
SphI          528  GCATGC               GCATG^C
SspBI         328  TGTACA               T^GTACA
StuI          752  AGGCCT               AGG^CCT
Van91I       1100  CCATGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2394  TCTAGA               T^CTAGA
XmnI         1191  GAATAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BanI         3097  GGCACC               G^G[CT][GA]CC
             3253  GGCACC              
BbsI         1483  GAAGAC               GAAGAC(2/6)
              366  GTCTTC              
BlpI          535  GCTCAGC              GC^T[ACGT]AGC
             2107  GCTCAGC             
Bpu1102I      535  GCTCAGC              GC^T[ACGT]AGC
             2107  GCTCAGC             
BpuAI        1483  GAAGAC               GAAGAC(2/6)
              366  GTCTTC              
BstAPI         99  GCAGGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1019  GCATCCTGTGC         
CelII         535  GCTCAGC              GC^T[ACGT]AGC
             2107  GCTCAGC             
DrdI         1731  GACTCCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2811  GACTCCGTGGTC        
EcoNI          24  CCTCCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              518  CCTACTATAGG         
FauI         1878  CCCGC                CCCGC(4/6)
             2205  CCCGC               
SmlI         1221  CTCAAG               C^T[CT][GA]AG
             1778  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BanII       BbvI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       
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