Restriction map for pF1KSDA0250
 (Linear) (Six-base) MAPSORT of: KIAA0250.seq  Check: 1512  from: 1  to: 3273



KIAA0250.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AccI GT'mk_AC

Cuts at:      0    2125    3273 
   Size:       2125    1148
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     101    1763    1933    2311    3273 
   Size:         64      37    1662     170     378     962

  Fragments arranged by size: 

                1662     962     378     170      64      37
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1126    3273 
   Size:       1126    2147
 
AleI CACnn'nnGTG

Cuts at:      0     923    3273 
   Size:        923    2350
 
AlwNI CAG_nnn'CTG

Cuts at:      0     105     114     993    1741    3273 
   Size:        105       9     879     748    1532

  Fragments arranged by size: 

                1532     879     748     105       9
 
ApoI r'AATT_y

Cuts at:      0     763    1593    2464    3273 
   Size:        763     830     871     809

  Fragments arranged by size: 

                 871     830     809     763
 
AseI AT'TA_AT

Cuts at:      0    1474    3273 
   Size:       1474    1799
 
BamHI G'GATC_C

Cuts at:      0    2577    3273 
   Size:       2577     696
 
BanI G'GyrC_C

Cuts at:      0    3097    3253    3273 
   Size:       3097     156      20

  Fragments arranged by size: 

                3097     156      20
 
BanII G_rGCy'C

Cuts at:      0     811    1561    1903    2633    3273 
   Size:        811     750     342     730     640

  Fragments arranged by size: 

                 811     750     730     640     342
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     359    1490    3273 
   Size:        359    1131    1783

  Fragments arranged by size: 

                1783    1131     359
 
BfrBI ATG'CAT

Cuts at:      0    3186    3273 
   Size:       3186      87
 
BglII A'GATC_T

Cuts at:      0    2503    3273 
   Size:       2503     770
 
BlpI GC'TnA_GC

Cuts at:      0     536    2108    3273 
   Size:        536    1572    1165

  Fragments arranged by size: 

                1572    1165     536
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1484    1788    2481    3273 
   Size:       1484     304     693     792

  Fragments arranged by size: 

                1484     792     693     304
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1236    2640    3150    3273 
   Size:       1236    1404     510     123

  Fragments arranged by size: 

                1404    1236     510     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1718    1838    1952    2948    3226    3273 
   Size:       1718     120     114     996     278      47

  Fragments arranged by size: 

                1718     996     278     120     114      47
 
Bpu10I CC'TnA_GC

Cuts at:      0    1902    3273 
   Size:       1902    1371
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1206    1763    3273 
   Size:       1206     557    1510

  Fragments arranged by size: 

                1510    1206     557
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     593    1101    1275    1667    2825    3273 
   Size:        593     508     174     392    1158     448

  Fragments arranged by size: 

                1158     593     508     448     392     174
 
BsaWI w'CCGG_w

Cuts at:      0    2665    3273 
   Size:       2665     608
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3199    3229    3273 
   Size:       3199      30      44

  Fragments arranged by size: 

                3199      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1072    1264    1820    2861    2897    3273 
   Size:       1072     192     556    1041      36     376

  Fragments arranged by size: 

                1072    1041     556     376     192      36
 
BseYI C'CCAG_C

Cuts at:      0      69     657    1837    1917    1990    2081    2899    3273 
   Size:         69     588    1180      80      73      91     818     374

  Fragments arranged by size: 

                1180     818     588     374      91      80      73      69
            
 
BsiHKAI G_wGCw'C

Cuts at:      0    2633    3273 
   Size:       2633     640
 
BsmI GAATG_Cn'

Cuts at:      0    2597    3273 
   Size:       2597     676
 
Bsp1286I G_dGCh'C

Cuts at:      0     811    1561    1903    2633    3273 
   Size:        811     750     342     730     640

  Fragments arranged by size: 

                 811     750     730     640     342
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2130    3273 
   Size:       2130    1143
 
BsrDI GCAATG_nn'

Cuts at:      0     479     635     942    2362    3273 
   Size:        479     156     307    1420     911

  Fragments arranged by size: 

                1420     911     479     307     156
 
BsrFI r'CCGG_y

Cuts at:      0    2187    3273 
   Size:       2187    1086
 
BsrGI T'GTAC_A

Cuts at:      0     328    3273 
   Size:        328    2945
 
BssHII G'CGCG_C

Cuts at:      0      14    3273 
   Size:         14    3259
 
BssSI C'ACGA_G

Cuts at:      0    1658    3273 
   Size:       1658    1615
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     105    1025    3273 
   Size:        105     920    2248

  Fragments arranged by size: 

                2248     920     105
 
BstEII G'GTnAC_C

Cuts at:      0    1315    1632    2549    3273 
   Size:       1315     317     917     724

  Fragments arranged by size: 

                1315     917     724     317
 
BstYI r'GATC_y

Cuts at:      0     174    2503    2577    3273 
   Size:        174    2329      74     696

  Fragments arranged by size: 

                2329     696     174      74
 
Bsu36I CC'TnA_GG

Cuts at:      0    2273    3273 
   Size:       2273    1000
 
BtgI C'CryG_G

Cuts at:      0     768    1100    2420    2815    3017    3111    3122    3273 
   Size:        768     332    1320     395     202      94      11     151

  Fragments arranged by size: 

                1320     768     395     332     202     151      94      11
            
 
BtsI GCAGTG_nn'

Cuts at:      0     456    1148    3273 
   Size:        456     692    2125

  Fragments arranged by size: 

                2125     692     456
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1737    2817    3273 
   Size:       1737    1080     456

  Fragments arranged by size: 

                1737    1080     456
 
EarI CTCTTCn'nnn_

Cuts at:      0     818    1085    1190    1205    1838    3273 
   Size:        818     267     105      15     633    1435

  Fragments arranged by size: 

                1435     818     633     267     105      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1844    3273 
   Size:       1844    1429
 
EcoICRI GAG'CTC

Cuts at:      0    2631    3273 
   Size:       2631     642
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     101    1718    1763    1838    1933    1952    2311 
   Size:         64      37    1617      45      75      95      19     359

Cuts at:   2311    2948    3226    3273 
   Size:        637     278      47

  Fragments arranged by size: 

                1617     637     359     278      95      75      64      47
                  45      37      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      28     522    3273 
   Size:         28     494    2751

  Fragments arranged by size: 

                2751     494      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111    1215    2056    2457    3273 
   Size:        111    1104     841     401     816

  Fragments arranged by size: 

                1104     841     816     401     111
 
EcoRI G'AATT_C

Cuts at:      0    2464    3273 
   Size:       2464     809
 
HaeII r_GCGC'y

Cuts at:      0     413    3273 
   Size:        413    2860
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1112    1144    1693    1725    2488    2520    2620    2652 
   Size:       1112      32     549      32     763      32     100      32

Cuts at:   2652    2660    2692    2976    3008    3028    3060    3273 
   Size:          8      32     284      32      20      32     213

  Fragments arranged by size: 

                1112     763     549     284     213     100      32      32
                  32      32      32      32      32      20       8
 
HindIII A'AGCT_T

Cuts at:      0     860    3273 
   Size:        860    2413
 
MfeI C'AATT_G

Cuts at:      0     373    3273 
   Size:        373    2900
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    2935    3273 
   Size:        961    1974     338

  Fragments arranged by size: 

                1974     961     338
 
MslI CAynn'nnrTG

Cuts at:      0     640     773     923    1205    3273 
   Size:        640     133     150     282    2068

  Fragments arranged by size: 

                2068     640     282     150     133
 
MspA1I CmG'CkG

Cuts at:      0      79     661    1921    2136    2259    3213    3273 
   Size:         79     582    1260     215     123     954      60

  Fragments arranged by size: 

                1260     954     582     215     123      79      60
 
NaeI GCC'GGC

Cuts at:      0    2189    3273 
   Size:       2189    1084
 
NcoI C'CATG_G

Cuts at:      0    1100    2420    3017    3111    3122    3273 
   Size:       1100    1320     597      94      11     151

  Fragments arranged by size: 

                1320    1100     597     151      94      11
 
NdeI CA'TA_TG

Cuts at:      0    1866    3273 
   Size:       1866    1407
 
NgoMIV G'CCGG_C

Cuts at:      0    2187    3273 
   Size:       2187    1086
 
NsiI A_TGCA'T

Cuts at:      0    3188    3273 
   Size:       3188      85
 
NspI r_CATG'y

Cuts at:      0     532    3273 
   Size:        532    2741
 
PasI CC'CwG_GG

Cuts at:      0    1124    1561    3273 
   Size:       1124     437    1712

  Fragments arranged by size: 

                1712    1124     437
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1106    3273 
   Size:       1106    2167
 
PfoI T'CCnGG_A

Cuts at:      0    3189    3273 
   Size:       3189      84
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     161     193    3273 
   Size:        161      32    3080

  Fragments arranged by size: 

                3080     161      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     111    1215    2056    2457    3273 
   Size:        111    1104     841     401     816

  Fragments arranged by size: 

                1104     841     816     401     111
 
PsiI TTA'TAA

Cuts at:      0     566    3273 
   Size:        566    2707
 
PstI C_TGCA'G

Cuts at:      0      11     110     631    1551    2141    2991    3273 
   Size:         11      99     521     920     590     850     282

  Fragments arranged by size: 

                 920     850     590     521     282      99      11
 
PvuII CAG'CTG

Cuts at:      0      79     661    1921    2136    2259    3213    3273 
   Size:         79     582    1260     215     123     954      60

  Fragments arranged by size: 

                1260     954     582     215     123      79      60
 
SacI G_AGCT'C

Cuts at:      0    2633    3273 
   Size:       2633     640
 
SexAI A'CCwGG_T

Cuts at:      0    1979    3273 
   Size:       1979    1294
 
SfcI C'TryA_G

Cuts at:      0       7     106     522     627    1039    1382    1547    1955 
   Size:          7      99     416     105     412     343     165     408

Cuts at:   1955    2137    2484    2987    3273 
   Size:        182     347     503     286

  Fragments arranged by size: 

                 503     416     412     408     347     343     286     182
                 165     105      99       7
 
SmlI C'TyrA_G

Cuts at:      0    1221    1778    3273 
   Size:       1221     557    1495

  Fragments arranged by size: 

                1495    1221     557
 
SphI G_CATG'C

Cuts at:      0     532    3273 
   Size:        532    2741
 
StuI AGG'CCT

Cuts at:      0     754    3273 
   Size:        754    2519
 
StyI C'CwwG_G

Cuts at:      0     471    1100    1411    1677    2420    3017    3111    3122 
   Size:        471     629     311     266     743     597      94      11

Cuts at:   3122    3273 
   Size:        151

  Fragments arranged by size: 

                 743     629     597     471     311     266     151      94
                  11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3010    3273 
   Size:       3010     263
 
TatI w'GTAC_w

Cuts at:      0     328    1160    3273 
   Size:        328     832    2113

  Fragments arranged by size: 

                2113     832     328
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1967    1999    3273 
   Size:       1967      32    1274

  Fragments arranged by size: 

                1967    1274      32
 
XbaI T'CTAG_A

Cuts at:      0    2394    3273 
   Size:       2394     879
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      83    1987    2468    3273 
   Size:         83    1904     481     805

  Fragments arranged by size: 

                1904     805     481      83
 
XmnI GAAnn'nnTTC

Cuts at:      0    1195    3273 
   Size:       1195    2078
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     AleI    AlwNI     ApoI     AseI    BamHI
     BanI    BanII     BbsI    BfrBI    BglII     BlpI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtgI     BtsI     DrdI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI     PpiI
    PpuMI     PsiI     PstI    PvuII     SacI    SexAI     SfcI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BciVI     BclI     BglI Bme1580I
    BmgBI     BmtI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BstBI    BstXI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     EaeI     EagI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     MscI     NarI     NheI     NotI     NruI     PacI     PciI     PmeI
     PmlI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I
     XhoI     XmaI     ZraI
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