Restriction map for pF1KSDA0248
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspI        
AvrII       BbrPI       BciVI       BlnI        BsaAI       
BsiWI       BsmBI       BspDI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      EcoRV       
EcoT22I     FseI        HpaI        KpnI        KspI        
MfeI        MluI        MunI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XhoI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI         4670  GGCGCC               G[GA]^CG[CT]C
AflII        4168  CTTAAG               C^TTAAG
AflIII       2621  ACATGT               A^C[GA][CT]GT
Alw44I       1077  GTGCAC               G^TGCAC
ApaLI        1077  GTGCAC               G^TGCAC
AviII        3671  TGCGCA               TGC^GCA
BbeI         4670  GGCGCC               GGCGC^C
BcgI         3525  GCAGACTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4168  CTTAAG               C^TTAAG
BglI         4085  GCCGCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3176  AGATCT               A^GATCT
BsaHI        4670  GGCGCC               G[GA]^CG[CT]C
BsiEI        1254  CGACCG               CG[GA][CT]^CG
BspHI        3401  TCATGA               T^CATGA
BspLU11I     2621  ACATGT               A^CATGT
BsrBI        3589  GAGCGG               CCGCTC(-3/-3)
BsrFI        4379  GCCGGC               [GA]^CCGG[CT]
BstEII        584  GGTTACC              G^GT[ACGT]ACC
BstPI         584  GGTTACC              G^GT[ACGT]ACC
Cfr10I       4379  GCCGGC               [GA]^CCGG[CT]
DraIII       3251  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3955  GACTCAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       584  GGTTACC              G^GT[ACGT]ACC
EcoRI         883  GAATTC               G^AATTC
FspI         3671  TGCGCA               TGC^GCA
HaeII        4670  GGCGCC               [GA]GCGC^[CT]
HgaI         4241  GCGTC                GACGC(5/10)
Hin1I        4670  GGCGCC               G[GA]^CG[CT]C
HindIII      1501  AAGCTT               A^AGCTT
KasI         4670  GGCGCC               G^GCGCC
NaeI         4379  GCCGGC               GCC^GGC
NarI         4670  GGCGCC               GG^CGCC
NgoMIV       4379  GCCGGC               G^CCGGC
NheI         1864  GCTAGC               G^CTAGC
PshAI         720  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         3401  TCATGA               T^CATGA
SfoI         4670  GGCGCC               GGC^GCC
SmaI         1139  CCCGGG               CCC^GGG
SspI           13  AATATT               AAT^ATT
XbaI          924  TCTAGA               T^CTAGA
XmaCI        1139  CCCGGG               C^CCGGG
XmaI         1139  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          556  GTAGAC               GT^[AC][GT]AC
             3964  GTCTAC              
AccII        1213  CGCG                 CG^CG
             4520  CGCG                
AhdI         4153  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4981  GACTTACTGTC         
AocI         2659  CCTGAGG              CC^T[ACGT]AGG
             2728  CCTGAGG             
AspEI        4153  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4981  GACTTACTGTC         
BclI         3356  TGATCA               T^GATCA
             4106  TGATCA              
BsaBI         399  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1488  GATGTACATC          
Bsp1407I     1490  TGTACA               T^GTACA
             4719  TGTACA              
BspMI        2981  ACCTGC               ACCTGC(4/8)
             5451  GCAGGT              
BsrDI        4862  CATTGC               GCAATG(2/0)
             3336  GCAATG              
BsrGI        1490  TGTACA               T^GTACA
             4719  TGTACA              
BstAPI       3871  GCACCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4506  GCACACCCTGC         
BstUI        1213  CGCG                 CG^CG
             4520  CGCG                
Bsu36I       2659  CCTGAGG              CC^T[ACGT]AGG
             2728  CCTGAGG             
DraI          147  TTTAAA               TTT^AAA
              595  TTTAAA              
Eam1105I     4153  GACACTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4981  GACTTACTGTC         
Eco81I       2659  CCTGAGG              CC^T[ACGT]AGG
             2728  CCTGAGG             
FbaI         3356  TGATCA               T^GATCA
             4106  TGATCA              
MamI          399  GATGAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1488  GATGTACATC          
MvnI         1213  CGCG                 CG^CG
             4520  CGCG                
NcoI         3975  CCATGG               C^CATGG
             5180  CCATGG              
NdeI          715  CATATG               CA^TATG
             3440  CATATG              
PpuMI        1015  GGGACCC              [GA]G^G[AT]CC[CT]
             5168  AGGACCC             
SexAI         732  ACCAGGT              A^CC[AT]GGT
             3719  ACCAGGT             
SphI          791  GCATGC               GCATG^C
             1457  GCATGC              
SspBI        1490  TGTACA               T^GTACA
             4719  TGTACA              
StuI         1926  AGGCCT               AGG^CCT
             2172  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccIII      AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspEI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MroI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI        
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