Restriction map for pF1KSDA0248
 (Linear) (Six-base) MAPSORT of: KIAA0248.seq  Check: 5158  from: 1  to: 5577



KIAA0248.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    5442    5577 
   Size:       5442     135
 
AccI GT'mk_AC

Cuts at:      0     557    3965    5577 
   Size:        557    3408    1612

  Fragments arranged by size: 

                3408    1612     557
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     394    1082    2295    2856    3769    5032    5577 
   Size:        242     152     688    1213     561     913    1263     545

  Fragments arranged by size: 

                1263    1213     913     688     561     545     242     152
            
 
AflII C'TTAA_G

Cuts at:      0    4168    5577 
   Size:       4168    1409
 
AflIII A'CryG_T

Cuts at:      0    2621    5577 
   Size:       2621    2956
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4158    4986    5577 
   Size:       4158     828     591

  Fragments arranged by size: 

                4158     828     591
 
AleI CACnn'nnGTG

Cuts at:      0    3554    3776    5577 
   Size:       3554     222    1801

  Fragments arranged by size: 

                3554    1801     222
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2758    4661    5577 
   Size:       2758    1903     916

  Fragments arranged by size: 

                2758    1903     916
 
AlwNI CAG_nnn'CTG

Cuts at:      0     343     841     967    1045    1324    2160    2325    3660 
   Size:        343     498     126      78     279     836     165    1335

Cuts at:   3660    4692    4890    5577 
   Size:       1032     198     687

  Fragments arranged by size: 

                1335    1032     836     687     498     343     279     198
                 165     126      78
 
ApaI G_GGCC'C

Cuts at:      0    2797    3286    3888    4082    4617    5577 
   Size:       2797     489     602     194     535     960

  Fragments arranged by size: 

                2797     960     602     535     489     194
 
ApaLI G'TGCA_C

Cuts at:      0    1077    5577 
   Size:       1077    4500
 
ApoI r'AATT_y

Cuts at:      0      66     883    2947    5577 
   Size:         66     817    2064    2630

  Fragments arranged by size: 

                2630    2064     817      66
 
AvaI C'yCGr_G

Cuts at:      0     991    1139    1856    3707    4409    5311    5321    5577 
   Size:        991     148     717    1851     702     902      10     256

  Fragments arranged by size: 

                1851     991     902     717     702     256     148      10
            
 
BamHI G'GATC_C

Cuts at:      0     108     372    3162    5577 
   Size:        108     264    2790    2415

  Fragments arranged by size: 

                2790    2415     264     108
 
BanI G'GyrC_C

Cuts at:      0     445    1852    2719    4625    4670    5577 
   Size:        445    1407     867    1906      45     907

  Fragments arranged by size: 

                1906    1407     907     867     445      45
 
BanII G_rGCy'C

Cuts at:      0     182    1220    1613    2797    3120    3286    3401    3737 
   Size:        182    1038     393    1184     323     166     115     336

Cuts at:   3737    3888    3905    4082    4238    4617    5320    5383    5577 
   Size:        151      17     177     156     379     703      63     194

  Fragments arranged by size: 

                1184    1038     703     393     379     336     323     194
                 182     177     166     156     151     115      63      17
            
 
BbeI G_GCGC'C

Cuts at:      0    4674    5577 
   Size:       4674     903
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     917    1192    3757    4869    5577 
   Size:        917     275    2565    1112     708

  Fragments arranged by size: 

                2565    1112     917     708     275
 
BbvCI CC'TCA_GC

Cuts at:      0     846    2908    5211    5577 
   Size:        846    2062    2303     366

  Fragments arranged by size: 

                2303    2062     846     366
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3514    3548    5577 
   Size:       3514      34    2029

  Fragments arranged by size: 

                3514    2029      34
 
BclI T'GATC_A

Cuts at:      0    3356    4106    5577 
   Size:       3356     750    1471

  Fragments arranged by size: 

                3356    1471     750
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4091    5577 
   Size:       4091    1486
 
BglII A'GATC_T

Cuts at:      0    3176    5577 
   Size:       3176    2401
 
BlpI GC'TnA_GC

Cuts at:      0    3229    5377    5411    5577 
   Size:       3229    2148      34     166

  Fragments arranged by size: 

                3229    2148     166      34
 
Bme1580I G_kGCm'C

Cuts at:      0     313     370     450     812    1081    2797    3286    3888 
   Size:        313      57      80     362     269    1716     489     602

Cuts at:   3888    4082    4617    4630    5525    5577 
   Size:        194     535      13     895      52

  Fragments arranged by size: 

                1716     895     602     535     489     362     313     269
                 194      80      57      52      13
 
BmgBI CAC'GTC

Cuts at:      0    1082    3927    5577 
   Size:       1082    2845    1650

  Fragments arranged by size: 

                2845    1650    1082
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     433    1029    1326    2981    3689    3795    3882    5577 
   Size:        433     596     297    1655     708     106      87    1695

  Fragments arranged by size: 

                1695    1655     708     596     433     297     106      87
            
 
BmtI G_CTAG'C

Cuts at:      0    1868    5577 
   Size:       1868    3709
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     716    1486    5161    5529    5577 
   Size:        716     770    3675     368      48

  Fragments arranged by size: 

                3675     770     716     368      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     926     991    1585    2011    2425    2572    2635    3361 
   Size:        926      65     594     426     414     147      63     726

Cuts at:   3361    3403    3821    3829    4377    4588    4959    5577 
   Size:         42     418       8     548     211     371     618

  Fragments arranged by size: 

                 926     726     618     594     548     426     418     414
                 371     211     147      65      63      42       8
 
Bpu10I CC'TnA_GC

Cuts at:      0     846    2908    3263    3481    3653    3822    5211    5577 
   Size:        846    2062     355     218     172     169    1389     366

  Fragments arranged by size: 

                2062    1389     846     366     355     218     172     169
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1333    1456    2857    5174    5346    5577 
   Size:       1333     123    1401    2317     172     231

  Fragments arranged by size: 

                2317    1401    1333     231     172     123
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3420    3530    4834    5577 
   Size:       3420     110    1304     743

  Fragments arranged by size: 

                3420    1304     743     110
 
BsaBI GATnn'nnATC

Cuts at:      0     403    1492    5577 
   Size:        403    1089    4085

  Fragments arranged by size: 

                4085    1089     403
 
BsaHI Gr'CG_yC

Cuts at:      0    4671    5577 
   Size:       4671     906
 
BsaWI w'CCGG_w

Cuts at:      0     494    3632    4253    5577 
   Size:        494    3138     621    1324

  Fragments arranged by size: 

                3138    1324     621     494
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1066    1096    2246    2276    2608    2638    4957    4987 
   Size:       1066      30    1150      30     332      30    2319      30

Cuts at:   4987    5577 
   Size:        590

  Fragments arranged by size: 

                2319    1150    1066     590     332      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     813     879    1087    2167    2569    2677    2854    3227 
   Size:        813      66     208    1080     402     108     177     373

Cuts at:   3227    4567    4852    5577 
   Size:       1340     285     725

  Fragments arranged by size: 

                1340    1080     813     725     402     373     285     208
                 177     108      66
 
BseYI C'CCAG_C

Cuts at:      0    1594    1802    4081    5286    5368    5577 
   Size:       1594     208    2279    1205      82     209

  Fragments arranged by size: 

                2279    1594    1205     209     208      82
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586    1597    4498    5078    5577 
   Size:        586    1011    2901     580     499

  Fragments arranged by size: 

                2901    1011     586     580     499
 
BsiEI CG_ry'CG

Cuts at:      0    1257    5577 
   Size:       1257    4320
 
BsiHKAI G_wGCw'C

Cuts at:      0      82     548    1081    1220    1469    1613    2308    3401 
   Size:         82     466     533     139     249     144     695    1093

Cuts at:   3401    3654    3905    4238    4713    5577 
   Size:        253     251     333     475     864

  Fragments arranged by size: 

                1093     864     695     533     475     466     333     253
                 251     249     144     139      82
 
BsmI GAATG_Cn'

Cuts at:      0    1689    2410    2541    5577 
   Size:       1689     721     131    3036

  Fragments arranged by size: 

                3036    1689     721     131
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     182     313     370     450     548     812    1081 
   Size:         82     100     131      57      80      98     264     269

Cuts at:   1081    1220    1469    1613    2308    2797    3120    3286    3401 
   Size:        139     249     144     695     489     323     166     115

Cuts at:   3401    3654    3737    3888    3905    4082    4238    4617    4630 
   Size:        253      83     151      17     177     156     379      13

Cuts at:   4630    4713    5320    5383    5525    5577 
   Size:         83     607      63     142      52

  Fragments arranged by size: 

                 695     607     489     379     323     269     264     253
                 249     177     166     156     151     144     142     139
                 131     115     100      98      83      83      82      80
                  63      57      52      17      13
 
BspEI T'CCGG_A

Cuts at:      0     494    3632    4253    5577 
   Size:        494    3138     621    1324

  Fragments arranged by size: 

                3138    1324     621     494
 
BspHI T'CATG_A

Cuts at:      0    3401    5577 
   Size:       3401    2176
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2990    5442    5577 
   Size:       2990    2452     135

  Fragments arranged by size: 

                2990    2452     135
 
BsrBI CCG'CTC

Cuts at:      0    3591    5577 
   Size:       3591    1986
 
BsrDI GCAATG_nn'

Cuts at:      0    3343    4861    5577 
   Size:       3343    1518     716

  Fragments arranged by size: 

                3343    1518     716
 
BsrFI r'CCGG_y

Cuts at:      0    4379    5577 
   Size:       4379    1198
 
BsrGI T'GTAC_A

Cuts at:      0    1490    4719    5577 
   Size:       1490    3229     858

  Fragments arranged by size: 

                3229    1490     858
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3877    4512    5577 
   Size:       3877     635    1065

  Fragments arranged by size: 

                3877    1065     635
 
BstEII G'GTnAC_C

Cuts at:      0     584    5577 
   Size:        584    4993
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2190    2550    3055    3315    4800    5181    5577 
   Size:       2190     360     505     260    1485     381     396

  Fragments arranged by size: 

                2190    1485     505     396     381     360     260
 
BstYI r'GATC_y

Cuts at:      0     108     294     372     497     694    2064    3162    3176 
   Size:        108     186      78     125     197    1370    1098      14

Cuts at:   3176    4256    5550    5577 
   Size:       1080    1294      27

  Fragments arranged by size: 

                1370    1294    1098    1080     197     186     125     108
                  78      27      14
 
Bsu36I CC'TnA_GG

Cuts at:      0    2660    2729    5577 
   Size:       2660      69    2848

  Fragments arranged by size: 

                2848    2660      69
 
BtgI C'CryG_G

Cuts at:      0    2068    3975    5180    5577 
   Size:       2068    1907    1205     397

  Fragments arranged by size: 

                2068    1907    1205     397
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1904    2474    2927    4676    5577 
   Size:       1904     570     453    1749     901

  Fragments arranged by size: 

                1904    1749     901     570     453
 
BtsI GCAGTG_nn'

Cuts at:      0     857     981    1770    1989    2970    3602    3802    4617 
   Size:        857     124     789     219     981     632     200     815

Cuts at:   4617    4897    5446    5577 
   Size:        280     549     131

  Fragments arranged by size: 

                 981     857     815     789     632     549     280     219
                 200     131     124
 
DraI TTT'AAA

Cuts at:      0     149     597    5577 
   Size:        149     448    4980

  Fragments arranged by size: 

                4980     448     149
 
DraIII CAC_nnn'GTG

Cuts at:      0    3256    5577 
   Size:       3256    2321
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3961    5577 
   Size:       3961    1616
 
EaeI y'GGCC_r

Cuts at:      0    3617    4578    5253    5531    5577 
   Size:       3617     961     675     278      46

  Fragments arranged by size: 

                3617     961     675     278      46
 
EarI CTCTTCn'nnn_

Cuts at:      0     580     685    1225    2782    3115    3188    3428    3635 
   Size:        580     105     540    1557     333      73     240     207

Cuts at:   3635    3802    5152    5577 
   Size:        167    1350     425

  Fragments arranged by size: 

                1557    1350     580     540     425     333     240     207
                 167     105      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2335    2883    4298    4564    5577 
   Size:       2335     548    1415     266    1013

  Fragments arranged by size: 

                2335    1415    1013     548     266
 
EcoICRI GAG'CTC

Cuts at:      0    1218    1611    3399    3903    4236    5577 
   Size:       1218     393    1788     504     333    1341

  Fragments arranged by size: 

                1788    1341    1218     504     393     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     394     926     991    1082    1585    2011    2295 
   Size:        242     152     532      65      91     503     426     284

Cuts at:   2295    2425    2572    2635    2856    3361    3403    3769    3821 
   Size:        130     147      63     221     505      42     366      52

Cuts at:   3821    3829    4377    4588    4959    5032    5577 
   Size:          8     548     211     371      73     545

  Fragments arranged by size: 

                 548     545     532     505     503     426     371     366
                 284     242     221     211     152     147     130      91
                  73      65      63      52      42       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1357    1654    3070    5577 
   Size:       1357     297    1416    2507

  Fragments arranged by size: 

                2507    1416    1357     297
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1016    2793    2794    3282    3884    4078    4093    5169 
   Size:       1016    1777       1     488     602     194      15    1076

Cuts at:   5169    5291    5577 
   Size:        122     286

  Fragments arranged by size: 

                1777    1076    1016     602     488     286     194     122
                  15       1
 
EcoRI G'AATT_C

Cuts at:      0     883    5577 
   Size:        883    4694
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1704    5577 
   Size:       1704    3873
 
FspI TGC'GCA

Cuts at:      0    3673    5577 
   Size:       3673    1904
 
HaeII r_GCGC'y

Cuts at:      0    4674    5577 
   Size:       4674     903
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     239     271    1066    1093    1098    1125    1359    1391 
   Size:        239      32     795      27       5      27     234      32

Cuts at:   1391    3187    3219    3629    3636    3661    3668    3892    3921 
   Size:       1796      32     410       7      25       7     224      29

Cuts at:   3921    3924    3953    4144    4176    4972    5004    5263    5295 
   Size:          3      29     191      32     796      32     259      32

Cuts at:   5295    5577 
   Size:        282

  Fragments arranged by size: 

                1796     796     795     410     282     259     239     234
                 224     191      32      32      32      32      32      32
                  29      29      27      27      25       7       7       5
                   3
 
HincII GTy'rAC

Cuts at:      0     267    1705    2004    3221    4026    5574    5577 
   Size:        267    1438     299    1217     805    1548       3

  Fragments arranged by size: 

                1548    1438    1217     805     299     267       3
 
HindIII A'AGCT_T

Cuts at:      0    1501    5577 
   Size:       1501    4076
 
KasI G'GCGC_C

Cuts at:      0    4670    5577 
   Size:       4670     907
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1670    2270    2404    3473    3977    5577 
   Size:       1670     600     134    1069     504    1600

  Fragments arranged by size: 

                1670    1600    1069     600     504     134
 
MscI TGG'CCA

Cuts at:      0    3619    4580    5255    5533    5577 
   Size:       3619     961     675     278      44

  Fragments arranged by size: 

                3619     961     675     278      44
 
MslI CAynn'nnrTG

Cuts at:      0     675     805    1519    2188    2717    3053    3554    3776 
   Size:        675     130     714     669     529     336     501     222

Cuts at:   3776    3920    4322    5179    5577 
   Size:        144     402     857     398

  Fragments arranged by size: 

                 857     714     675     669     529     501     402     398
                 336     222     144     130
 
MspA1I CmG'CkG

Cuts at:      0     570    3189    3381    3841    5041    5230    5577 
   Size:        570    2619     192     460    1200     189     347

  Fragments arranged by size: 

                2619    1200     570     460     347     192     189
 
NaeI GCC'GGC

Cuts at:      0    4381    5577 
   Size:       4381    1196
 
NarI GG'CG_CC

Cuts at:      0    4671    5577 
   Size:       4671     906
 
NcoI C'CATG_G

Cuts at:      0    3975    5180    5577 
   Size:       3975    1205     397

  Fragments arranged by size: 

                3975    1205     397
 
NdeI CA'TA_TG

Cuts at:      0     716    3441    5577 
   Size:        716    2725    2136

  Fragments arranged by size: 

                2725    2136     716
 
NgoMIV G'CCGG_C

Cuts at:      0    4379    5577 
   Size:       4379    1198
 
NheI G'CTAG_C

Cuts at:      0    1864    5577 
   Size:       1864    3713
 
NspI r_CATG'y

Cuts at:      0     795    1461    2625    4405    4965    5022    5577 
   Size:        795     666    1164    1780     560      57     555

  Fragments arranged by size: 

                1780    1164     795     666     560     555      57
 
PciI A'CATG_T

Cuts at:      0    2621    5577 
   Size:       2621    2956
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1122    1966    2789    5577 
   Size:       1122     844     823    2788

  Fragments arranged by size: 

                2788    1122     844     823
 
PfoI T'CCnGG_A

Cuts at:      0    1007    2612    5165    5577 
   Size:       1007    1605    2553     412

  Fragments arranged by size: 

                2553    1605    1007     412
 
PpuMI rG'GwC_Cy

Cuts at:      0    1016    5169    5577 
   Size:       1016    4153     408

  Fragments arranged by size: 

                4153    1016     408
 
PshAI GACnn'nnGTC

Cuts at:      0     724    5577 
   Size:        724    4853
 
PspOMI G'GGCC_C

Cuts at:      0    2793    3282    3884    4078    4613    5577 
   Size:       2793     489     602     194     535     964

  Fragments arranged by size: 

                2793     964     602     535     489     194
 
PstI C_TGCA'G

Cuts at:      0    3107    3851    4640    4706    4821    4886    5432    5453 
   Size:       3107     744     789      66     115      65     546      21

Cuts at:   5453    5577 
   Size:        124

  Fragments arranged by size: 

                3107     789     744     546     124     115      66      65
                  21
 
PvuII CAG'CTG

Cuts at:      0     570    3381    3841    5577 
   Size:        570    2811     460    1736

  Fragments arranged by size: 

                2811    1736     570     460
 
SacI G_AGCT'C

Cuts at:      0    1220    1613    3401    3905    4238    5577 
   Size:       1220     393    1788     504     333    1339

  Fragments arranged by size: 

                1788    1339    1220     504     393     333
 
SanDI GG'GwC_CC

Cuts at:      0    1016    5577 
   Size:       1016    4561
 
SapI GCTCTTCn'nnn_

Cuts at:      0     580    1225    3115    5577 
   Size:        580     645    1890    2462

  Fragments arranged by size: 

                2462    1890     645     580
 
SexAI A'CCwGG_T

Cuts at:      0     732    3719    5577 
   Size:        732    2987    1858

  Fragments arranged by size: 

                2987    1858     732
 
SfcI C'TryA_G

Cuts at:      0     866     959    1325    1901    3103    3184    3391    3847 
   Size:        866      93     366     576    1202      81     207     456

Cuts at:   3847    4536    4636    4702    4817    4882    5428    5449    5577 
   Size:        689     100      66     115      65     546      21     128

  Fragments arranged by size: 

                1202     866     689     576     546     456     366     207
                 128     115     100      93      81      66      65      21
            
 
SfoI GGC'GCC

Cuts at:      0    4672    5577 
   Size:       4672     905
 
SmaI CCC'GGG

Cuts at:      0    1141    5577 
   Size:       1141    4436
 
SmlI C'TyrA_G

Cuts at:      0    1312    1471    2836    4168    5189    5361    5577 
   Size:       1312     159    1365    1332    1021     172     216

  Fragments arranged by size: 

                1365    1332    1312    1021     216     172     159
 
SphI G_CATG'C

Cuts at:      0     795    1461    5577 
   Size:        795     666    4116

  Fragments arranged by size: 

                4116     795     666
 
SspI AAT'ATT

Cuts at:      0      15    5577 
   Size:         15    5562
 
StuI AGG'CCT

Cuts at:      0    1928    2174    5577 
   Size:       1928     246    3403

  Fragments arranged by size: 

                3403    1928     246
 
StyI C'CwwG_G

Cuts at:      0     905    3131    3975    5180    5405    5435    5577 
   Size:        905    2226     844    1205     225      30     142

  Fragments arranged by size: 

                2226    1205     905     844     225     142      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      97     118     316     364     433    1816    2556    4816 
   Size:         97      21     198      48      69    1383     740    2260

Cuts at:   4816    5577 
   Size:        761

  Fragments arranged by size: 

                2260    1383     761     740     198      97      69      48
                  21
 
TatI w'GTAC_w

Cuts at:      0    1490    4719    4958    5577 
   Size:       1490    3229     239     619

  Fragments arranged by size: 

                3229    1490     619     239
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1354    1386    2246    2278    2375    2407    4957    4989 
   Size:       1354      32     860      32      97      32    2550      32

Cuts at:   4989    5209    5241    5339    5371    5577 
   Size:        220      32      98      32     206

  Fragments arranged by size: 

                2550    1354     860     220     206      98      97      32
                  32      32      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     924    5577 
   Size:        924    4653
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     615    3727    4303    5577 
   Size:        615    3112     576    1274

  Fragments arranged by size: 

                3112    1274     615     576
 
XmaI C'CCGG_G

Cuts at:      0    1139    5577 
   Size:       1139    4438
 
XmnI GAAnn'nnTTC

Cuts at:      0     212    1687    4342    5577 
   Size:        212    1475    2655    1235

  Fragments arranged by size: 

                2655    1475    1235     212
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NspI
     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI    SexAI     SfcI     SfoI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI    BfrBI    BsaAI    BsiWI    BsmBI
   BssHII    BssSI    BstBI  BstZ17I     ClaI    CspCI     EagI    EcoRV
     FseI    FspAI     HpaI     KpnI     MfeI     MluI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XhoI
     ZraI
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