Restriction map for pF1KSDA0237
 (Circular) (Six-base) MAPSORT of: pF1KSDA0237.seq  Check: 2904  from: 1  to: 4063



pF1KSDA0237  4063 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    828     828 
   Size:       4063
 
AccI GT'mk_AC

Cuts at:   1099    1099 
   Size:       4063
 
Acc65I G'GTAC_C

Cuts at:   1077    1077 
   Size:       4063
 
AclI AA'CG_TT

Cuts at:   3903    3903 
   Size:       4063
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    362    1886    2318    3067    3335     362 
   Size:       1524     432     749     268    1090

  Fragments arranged by size: 

                1524    1090     749     432     268
 
AflIII A'CryG_T

Cuts at:   1413    2519    3496    1413 
   Size:       1106     977    1980

  Fragments arranged by size: 

                1980    1106     977
 
AgeI A'CCGG_T

Cuts at:   2451    2451 
   Size:       4063
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    850     850 
   Size:       4063
 
AlwNI CAG_nnn'CTG

Cuts at:    148     579     801    1366    2935     148 
   Size:        431     222     565    1569    1276

  Fragments arranged by size: 

                1569    1276     565     431     222
 
ApaLI G'TGCA_C

Cuts at:    604    2833     604 
   Size:       2229    1834
 
ApoI r'AATT_y

Cuts at:   1065    3416    3672    1065 
   Size:       2351     256    1456

  Fragments arranged by size: 

                2351    1456     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4063
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4063
 
AvaI C'yCGr_G

Cuts at:    659    1081     659 
   Size:        422    3641
 
BamHI G'GATC_C

Cuts at:    211     988    1086     211 
   Size:        777      98    3188

  Fragments arranged by size: 

                3188     777      98
 
BanI G'GyrC_C

Cuts at:    274    1001    1077    1491    1739    1774     274 
   Size:        727      76     414     248      35    2563

  Fragments arranged by size: 

                2563     727     414     248      76      35
 
BanII G_rGCy'C

Cuts at:    187    1075    2105     187 
   Size:        888    1030    2145

  Fragments arranged by size: 

                2145    1030     888
 
BbeI G_GCGC'C

Cuts at:   1005    1495    1743    1005 
   Size:        490     248    3325

  Fragments arranged by size: 

                3325     490     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    760     778    1007     760 
   Size:         18     229    3816

  Fragments arranged by size: 

                3816     229      18
 
BbvCI CC'TCA_GC

Cuts at:    922     922 
   Size:       4063
 
BciVI GTATCCnnnnn_n'

Cuts at:   1959    2722    4063    1959 
   Size:        763    1341    1959

  Fragments arranged by size: 

                1959    1341     763
 
BclI T'GATC_A

Cuts at:   1581    1581 
   Size:       4063
 
BglI GCCn_nnn'nGGC

Cuts at:    742     742 
   Size:       4063
 
BglII A'GATC_T

Cuts at:   1576    3615    1576 
   Size:       2039    2024
 
BlpI GC'TnA_GC

Cuts at:   1173    1173 
   Size:       4063
 
Bme1580I G_kGCm'C

Cuts at:    608    1686    1779    2837     608 
   Size:       1078      93    1058    1834

  Fragments arranged by size: 

                1834    1078    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1005    1273    1354    1689    3835    1005 
   Size:        268      81     335    2146    1233

  Fragments arranged by size: 

                2146    1233     335     268      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2428    2428 
   Size:       4063
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    150     171     310     658     745    1042     150 
   Size:         21     139     348      87     297    3171

  Fragments arranged by size: 

                3171     348     297     139      87      21
 
Bpu10I CC'TnA_GC

Cuts at:    922     922 
   Size:       4063
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1237    2116    2610    2908    1237 
   Size:        879     494     298    2392

  Fragments arranged by size: 

                2392     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3767    3767 
   Size:       4063
 
BsaAI yAC'GTr

Cuts at:   1053    1414    1053 
   Size:        361    3702
 
BsaBI GATnn'nnATC

Cuts at:      6    1575    3614       6 
   Size:       1569    2039     455

  Fragments arranged by size: 

                2039    1569     455
 
BsaHI Gr'CG_yC

Cuts at:   1002    1492    1740    2444    3940    1002 
   Size:        490     248     704    1496    1125

  Fragments arranged by size: 

                1496    1125     704     490     248
 
BsaWI w'CCGG_w

Cuts at:    623    1250    1771    2451    2725    2872    3283     623 
   Size:        627     521     680     274     147     411    1403

  Fragments arranged by size: 

                1403     680     627     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    954     954 
   Size:       4063
 
BseYI C'CCAG_C

Cuts at:    215     235     546     696    1486    2244    2823    3319     215 
   Size:         20     311     150     790     758     579     496     959

  Fragments arranged by size: 

                 959     790     758     579     496     311     150      20
            
 
BsiEI CG_ry'CG

Cuts at:    104    1603    1649    2859     104 
   Size:       1499      46    1210    1308

  Fragments arranged by size: 

                1499    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    187     322     385     460     608    1075    1853    2043    2837 
   Size:        135      63      75     148     467     778     190     794

Cuts at:   2837     187 
   Size:       1413

  Fragments arranged by size: 

                1413     794     778     467     190     148     135      75
                  63
 
Bsp1286I G_dGCh'C

Cuts at:    187     322     385     460     608    1075    1686    1779    1853 
   Size:        135      63      75     148     467     611      93      74

Cuts at:   1853    2043    2105    2837     187 
   Size:        190      62     732    1413

  Fragments arranged by size: 

                1413     732     611     467     190     148     135      93
                  75      74      63      62
 
BspEI T'CCGG_A

Cuts at:   3283    3283 
   Size:       4063
 
BspHI T'CATG_A

Cuts at:   3239    3239 
   Size:       4063
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    728     828    1111    1325    1627    2008     728 
   Size:        100     283     214     302     381    2783

  Fragments arranged by size: 

                2783     381     302     283     214     100
 
BsrBI CCG'CTC

Cuts at:     50    2353    2407    3893    4058      50 
   Size:       2303      54    1486     165      55

  Fragments arranged by size: 

                2303    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    608    1973     608 
   Size:       1365    2698
 
BsrFI r'CCGG_y

Cuts at:    937    2059    2240    2451     937 
   Size:       1122     181     211    2549

  Fragments arranged by size: 

                2549    1122     211     181
 
BssSI C'ACGA_G

Cuts at:   2332    2692    2332 
   Size:        360    3703
 
BstAPI GCAn_nnn'nTGC

Cuts at:    801    1438     801 
   Size:        637    3426
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4063
 
BstEII G'GTnAC_C

Cuts at:    933     933 
   Size:       4063
 
BstXI CCAn_nnnn'nTGG

Cuts at:    174     285     174 
   Size:        111    3952
 
BstYI r'GATC_y

Cuts at:      1     211     988    1086    1553    1576    1911    2157    3171 
   Size:        210     777      98     467      23     335     246    1014

Cuts at:   3171    3257    3269    3615       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1014     777     467     449     346     335     246     210
                  98      86      23      12
 
BtgI C'CryG_G

Cuts at:    593     929     593 
   Size:        336    3727
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    381    2011    2192    2355     381 
   Size:       1630     181     163    2089

  Fragments arranged by size: 

                2089    1630     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1304    1304 
   Size:       4063
 
DraI TTT'AAA

Cuts at:   1060    1060 
   Size:       4063
 
DrdI GACnn_nn'nnGTC

Cuts at:   1767    2627    1767 
   Size:        860    3203
 
EaeI y'GGCC_r

Cuts at:    287     630     743     861    1646    1820    2211    2238    3585 
   Size:        343     113     118     785     174     391      27    1347

Cuts at:   3585     287 
   Size:        765

  Fragments arranged by size: 

                1347     785     765     391     343     174     118     113
                  27
 
EagI C'GGCC_G

Cuts at:   1646    1646 
   Size:       4063
 
EarI CTCTTCn'nnn_

Cuts at:    958    2084    2294     958 
   Size:       1126     210    2727

  Fragments arranged by size: 

                2727    1126     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    276     364    2269    2581    2727    3873     276 
   Size:         88    1905     312     146    1146     466

  Fragments arranged by size: 

                1905    1146     466     312     146      88
 
EcoICRI GAG'CTC

Cuts at:    185    1073     185 
   Size:        888    3175
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    150     171     310     362     658     745    1042    1886    2318 
   Size:         21     139      52     296      87     297     844     432

Cuts at:   2318    3067    3335     150 
   Size:        749     268     878

  Fragments arranged by size: 

                 878     844     749     432     297     296     268     139
                  87      52      21
 
EcoO109I rG'GnC_Cy

Cuts at:    145     161     663     734     817    1200     145 
   Size:         16     502      71      83     383    3008

  Fragments arranged by size: 

                3008     502     383      83      71      16
 
EcoRI G'AATT_C

Cuts at:   1065    1065 
   Size:       4063
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2080    2080 
   Size:       4063
 
FspI TGC'GCA

Cuts at:    364    1842     364 
   Size:       1478    2585
 
HaeII r_GCGC'y

Cuts at:    929    1005    1495    1743    2767    3746     929 
   Size:         76     490     248    1024     979    1246

  Fragments arranged by size: 

                1246    1024     979     490     248      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    642     674     765     797    1903    1933    1935    1965     642 
   Size:         32      91      32    1106      30       2      30    2740

  Fragments arranged by size: 

                2740    1106      91      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1100    1100 
   Size:       4063
 
KasI G'GCGC_C

Cuts at:   1001    1491    1739    1001 
   Size:        490     248    3325

  Fragments arranged by size: 

                3325     490     248
 
KpnI G_GTAC'C

Cuts at:   1081    1081 
   Size:       4063
 
MluI A'CGCG_T

Cuts at:   3496    3496 
   Size:       4063
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    500    2734    2918     500 
   Size:       2234     184    1645

  Fragments arranged by size: 

                2234    1645     184
 
MscI TGG'CCA

Cuts at:    289     632     745    1822     289 
   Size:        343     113    1077    2530

  Fragments arranged by size: 

                2530    1077     343     113
 
MslI CAynn'nnrTG

Cuts at:    400     889    2050    2177     400 
   Size:        489    1161     127    2286

  Fragments arranged by size: 

                2286    1161     489     127
 
MspA1I CmG'CkG

Cuts at:    636     931    1172    1486    1846    2861    3106     636 
   Size:        295     241     314     360    1015     245    1593

  Fragments arranged by size: 

                1593    1015     360     314     295     245     241
 
NaeI GCC'GGC

Cuts at:   2242    2242 
   Size:       4063
 
NarI GG'CG_CC

Cuts at:   1002    1492    1740    1002 
   Size:        490     248    3325

  Fragments arranged by size: 

                3325     490     248
 
NcoI C'CATG_G

Cuts at:    593     593 
   Size:       4063
 
NgoMIV G'CCGG_C

Cuts at:   2240    2240 
   Size:       4063
 
NruI TCG'CGA

Cuts at:   2424    2424 
   Size:       4063
 
NspI r_CATG'y

Cuts at:   1114    1614    2523    1114 
   Size:        500     909    2654

  Fragments arranged by size: 

                2654     909     500
 
PasI CC'CwG_GG

Cuts at:    822     822 
   Size:       4063
 
PciI A'CATG_T

Cuts at:   2519    2519 
   Size:       4063
 
PfoI T'CCnGG_A

Cuts at:    166     536     166 
   Size:        370    3693
 
PmeI GTTT'AAAC

Cuts at:   1060    1060 
   Size:       4063
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3202    3234    3202 
   Size:         32    4031
 
PpuMI rG'GwC_Cy

Cuts at:    145     817     145 
   Size:        672    3391
 
PstI C_TGCA'G

Cuts at:    839    1108    1793     839 
   Size:        269     685    3109

  Fragments arranged by size: 

                3109     685     269
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4063
 
PvuII CAG'CTG

Cuts at:    636    1486    1846     636 
   Size:        850     360    2853

  Fragments arranged by size: 

                2853     850     360
 
RsrII CG'GwC_CG

Cuts at:   2256    2256 
   Size:       4063
 
SacI G_AGCT'C

Cuts at:    187    1075     187 
   Size:        888    3175
 
SacII CC_GC'GG

Cuts at:    932     932 
   Size:       4063
 
SalI G'TCGA_C

Cuts at:   1098    1098 
   Size:       4063
 
SanDI GG'GwC_CC

Cuts at:    817     817 
   Size:       4063
 
SapI GCTCTTCn'nnn_

Cuts at:   2084    2294    2084 
   Size:        210    3853
 
SbfI CC_TGCA'GG

Cuts at:   1108    1108 
   Size:       4063
 
ScaI AGT'ACT

Cuts at:   3580    3580 
   Size:       4063
 
SfcI C'TryA_G

Cuts at:     33     835    1104    1789    2784    2975      33 
   Size:        802     269     685     995     191    1121

  Fragments arranged by size: 

                1121     995     802     685     269     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    742     742 
   Size:       4063
 
SfoI GGC'GCC

Cuts at:   1003    1493    1741    1003 
   Size:        490     248    3325

  Fragments arranged by size: 

                3325     490     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4063
 
SmaI CCC'GGG

Cuts at:   1083    1083 
   Size:       4063
 
SmlI C'TyrA_G

Cuts at:   1216    2131    2625    2887    1216 
   Size:        915     494     262    2392

  Fragments arranged by size: 

                2392     915     494     262
 
SnaBI TAC'GTA

Cuts at:   1053    1053 
   Size:       4063
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4063
 
SphI G_CATG'C

Cuts at:   1114    1614    1114 
   Size:        500    3563
 
StyI C'CwwG_G

Cuts at:    593    1195    1549     593 
   Size:        602     354    3107

  Fragments arranged by size: 

                3107     602     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    262     606    1644    1777    2236    2447     262 
   Size:        344    1038     133     459     211    1878

  Fragments arranged by size: 

                1878    1038     459     344     211     133
 
TatI w'GTAC_w

Cuts at:   3578    3578 
   Size:       4063
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    862     894    3580    3612     862 
   Size:         32    2686      32    1313

  Fragments arranged by size: 

                2686    1313      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1858    1858 
   Size:       4063
 
XbaI T'CTAG_A

Cuts at:   1092    1092 
   Size:       4063
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    966    1556     966 
   Size:        590    3473
 
XmaI C'CCGG_G

Cuts at:   1081    1081 
   Size:       4063
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NruI     NspI     PasI     PciI     PfoI     PmeI
     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI    BfrBI    BmgBI     BmtI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NsiI     PacI    PflMI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SexAI    SgrAI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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