Restriction map for
pF1KSDA0222
(Circular) (Six-base) MAPSORT of: pF1KSDA0222.seq Check: 8187 from: 1 to: 6626
pF1KSDA0222 6626 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 238 238
Size: 6626
AccI GT'mk_AC
Cuts at: 2341 3664 2341
Size: 1323 5303
Acc65I G'GTAC_C
Cuts at: 3580 3642 3580
Size: 62 6564
AclI AA'CG_TT
Cuts at: 6466 6466
Size: 6626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 879 934 2883 3542 4451 4883 5630 5898
Size: 503 55 1949 659 909 432 747 268
Cuts at: 5898 376
Size: 1104
Fragments arranged by size:
1949 1104 909 747 659 503 432 268
55
AflIII A'CryG_T
Cuts at: 1728 3978 5082 6059 1728
Size: 2250 1104 977 2295
Fragments arranged by size:
2295 2250 1104 977
AgeI A'CCGG_T
Cuts at: 2871 5014 2871
Size: 2143 4483
AleI CACnn'nnGTG
Cuts at: 2641 2641
Size: 6626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 520 595 2659 520
Size: 75 2064 4487
Fragments arranged by size:
4487 2064 75
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 976 1008 976
Size: 32 6594
AlwNI CAG_nnn'CTG
Cuts at: 1284 2071 3153 3931 5498 1284
Size: 787 1082 778 1567 2412
Fragments arranged by size:
2412 1567 1082 787 778
ApaI G_GGCC'C
Cuts at: 812 1253 1553 1788 2578 2837 3471 812
Size: 441 300 235 790 259 634 3967
Fragments arranged by size:
3967 790 634 441 300 259 235
ApaLI G'TGCA_C
Cuts at: 703 5396 703
Size: 4693 1933
ApoI r'AATT_y
Cuts at: 3055 3630 5979 6235 3055
Size: 575 2349 256 3446
Fragments arranged by size:
3446 2349 575 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6626
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6626
AvaI C'yCGr_G
Cuts at: 624 847 859 1866 2440 2883 2936 3512 3646
Size: 223 12 1007 574 443 53 576 134
Cuts at: 3646 624
Size: 3604
Fragments arranged by size:
3604 1007 576 574 443 223 134 53
12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3564 3597 3564
Size: 33 6593
BamHI G'GATC_C
Cuts at: 3651 3651
Size: 6626
BanI G'GyrC_C
Cuts at: 423 532 1915 2175 2471 2643 2760 2874 3228
Size: 109 1383 260 296 172 117 114 354
Cuts at: 3228 3580 3642 4056 4304 4339 423
Size: 352 62 414 248 35 2710
Fragments arranged by size:
2710 1383 414 354 352 296 260 248
172 117 114 109 62 35
BanII G_rGCy'C
Cuts at: 268 812 859 943 989 1253 1553 1788 1873
Size: 544 47 84 46 264 300 235 85
Cuts at: 1873 1988 2578 2837 3131 3471 3640 4670 268
Size: 115 590 259 294 340 169 1030 2224
Fragments arranged by size:
2224 1030 590 544 340 300 294 264
259 235 169 115 85 84 47 46
BbeI G_GCGC'C
Cuts at: 427 536 1919 2764 3232 4060 4308 427
Size: 109 1383 845 468 828 248 2745
Fragments arranged by size:
2745 1383 845 828 468 248 109
BbsI GAAGACnn'nnnn_
Cuts at: 1904 1904
Size: 6626
BbvCI CC'TCA_GC
Cuts at: 157 3154 157
Size: 2997 3629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1873 1907 1873
Size: 34 6592
BciVI GTATCCnnnnn_n'
Cuts at: 4524 5285 6626 4524
Size: 761 1341 4524
Fragments arranged by size:
4524 1341 761
BclI T'GATC_A
Cuts at: 4146 4146
Size: 6626
BglI GCCn_nnn'nGGC
Cuts at: 523 559 1414 1888 3205 523
Size: 36 855 474 1317 3944
Fragments arranged by size:
3944 1317 855 474 36
BglII A'GATC_T
Cuts at: 1691 4141 6178 1691
Size: 2450 2037 2139
Fragments arranged by size:
2450 2139 2037
BlpI GC'TnA_GC
Cuts at: 772 853 1349 3065 3738 772
Size: 81 496 1716 673 3660
Fragments arranged by size:
3660 1716 673 496 81
Bme1580I G_kGCm'C
Cuts at: 707 812 1253 1321 1553 1788 2180 2578 2837
Size: 105 441 68 232 235 392 398 259
Cuts at: 2837 3036 3471 3483 4251 4344 5400 707
Size: 199 435 12 768 93 1056 1933
Fragments arranged by size:
1933 1056 768 441 435 398 392 259
235 232 199 105 93 68 12
BmgBI CAC'GTC
Cuts at: 1464 1464
Size: 6626
BmrI ACTGGGnnnn_n'
Cuts at: 3838 3919 4254 6398 3838
Size: 81 335 2144 4066
Fragments arranged by size:
4066 2144 335 81
BmtI G_CTAG'C
Cuts at: 2711 2864 2711
Size: 153 6473
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1269 1830 2391 2880 3150 3406 4993 1269
Size: 561 561 489 270 256 1587 2902
Fragments arranged by size:
2902 1587 561 561 489 270 256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 1261 1974 712
Size: 549 713 5364
Fragments arranged by size:
5364 713 549
Bpu10I CC'TnA_GC
Cuts at: 157 1117 3154 157
Size: 960 2037 3629
Fragments arranged by size:
3629 2037 960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1411 3802 4681 5173 5471 982
Size: 429 2391 879 492 298 2137
Fragments arranged by size:
2391 2137 879 492 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 1991 3557 6330 1991
Size: 1566 2773 2287
Fragments arranged by size:
2773 2287 1566
BsaAI yAC'GTr
Cuts at: 702 3618 3979 702
Size: 2916 361 3349
Fragments arranged by size:
3349 2916 361
BsaBI GATnn'nnATC
Cuts at: 6 364 4140 6177 6
Size: 358 3776 2037 455
Fragments arranged by size:
3776 2037 455 358
BsaHI Gr'CG_yC
Cuts at: 424 461 533 1916 2761 3229 3545 4057 4305
Size: 37 72 1383 845 468 316 512 248
Cuts at: 4305 5007 6503 424
Size: 702 1496 547
Fragments arranged by size:
1496 1383 845 702 547 512 468 316
248 72 37
BsaWI w'CCGG_w
Cuts at: 368 2737 2871 3392 3815 4336 5014 5288 5435
Size: 2369 134 521 423 521 678 274 147
Cuts at: 5435 5846 368
Size: 411 1148
Fragments arranged by size:
2369 1148 678 521 521 423 411 274
147 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 925 955 976 1006 1804 1834 3404
Size: 30 39 30 21 30 798 30 1570
Cuts at: 3404 3434 856
Size: 30 4048
Fragments arranged by size:
4048 1570 798 39 30 30 30 30
30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 775 1111 1249 1924 2367 3098 3101 775
Size: 336 138 675 443 731 3 4300
Fragments arranged by size:
4300 731 675 443 336 138 3
BseYI C'CCAG_C
Cuts at: 169 429 1823 1828 2356 2994 3209 3223 4051
Size: 260 1394 5 528 638 215 14 828
Cuts at: 4051 4809 5386 5882 169
Size: 758 577 496 913
Fragments arranged by size:
1394 913 828 758 638 577 528 496
260 215 14 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 750 759 1705 1913 682
Size: 68 9 946 208 5395
Fragments arranged by size:
5395 946 208 68 9
BsiEI CG_ry'CG
Cuts at: 104 189 972 1232 1307 1418 1619 1982 4168
Size: 85 783 260 75 111 201 363 2186
Cuts at: 4168 4214 5422 104
Size: 46 1208 1308
Fragments arranged by size:
2186 1308 1208 783 363 260 201 111
85 75 46
BsiHKAI G_wGCw'C
Cuts at: 268 655 707 1988 3066 3380 3640 4418 4608
Size: 387 52 1281 1078 314 260 778 190
Cuts at: 4608 5400 268
Size: 792 1494
Fragments arranged by size:
1494 1281 1078 792 778 387 314 260
190 52
BsmI GAATG_Cn'
Cuts at: 2608 2608
Size: 6626
BsmBI CGTCTCn'nnnn_
Cuts at: 2362 2362
Size: 6626
Bsp1286I G_dGCh'C
Cuts at: 268 655 707 812 859 943 989 1253 1321
Size: 387 52 105 47 84 46 264 68
Cuts at: 1321 1553 1788 1873 1988 2180 2578 2837 3036
Size: 232 235 85 115 192 398 259 199
Cuts at: 3036 3066 3131 3380 3471 3483 3640 4251 4344
Size: 30 65 249 91 12 157 611 93
Cuts at: 4344 4418 4608 4670 5400 268
Size: 74 190 62 730 1494
Fragments arranged by size:
1494 730 611 398 387 264 259 249
235 232 199 192 190 157 115 105
93 91 85 84 74 68 65 62
52 47 46 30 12
BspEI T'CCGG_A
Cuts at: 368 2737 3392 5846 368
Size: 2369 655 2454 1148
Fragments arranged by size:
2454 2369 1148 655
BspHI T'CATG_A
Cuts at: 5802 5802
Size: 6626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 229 238 1935 3676 3890 4192 4573 229
Size: 9 1697 1741 214 302 381 2282
Fragments arranged by size:
2282 1741 1697 381 302 214 9
BsrBI CCG'CTC
Cuts at: 50 1571 1613 2221 2564 4918 4972 6456 6621
Size: 1521 42 608 343 2354 54 1484 165
Cuts at: 6621 50
Size: 55
Fragments arranged by size:
2354 1521 1484 608 343 165 55 54
42
BsrDI GCAATG_nn'
Cuts at: 4538 4538
Size: 6626
BsrFI r'CCGG_y
Cuts at: 184 500 1111 1306 1413 1594 1651 2041 2871
Size: 316 611 195 107 181 57 390 830
Cuts at: 2871 4624 4805 5014 184
Size: 1753 181 209 1796
Fragments arranged by size:
1796 1753 830 611 390 316 209 195
181 181 107 57
BssHII G'CGCG_C
Cuts at: 441 1221 1563 1621 1742 1744 2945 441
Size: 780 342 58 121 2 1201 4122
Fragments arranged by size:
4122 1201 780 342 121 58 2
BssSI C'ACGA_G
Cuts at: 196 403 1314 4897 5255 196
Size: 207 911 3583 358 1567
Fragments arranged by size:
3583 1567 911 358 207
BstAPI GCAn_nnn'nTGC
Cuts at: 282 737 2175 2603 3431 4003 282
Size: 455 1438 428 828 572 2905
Fragments arranged by size:
2905 1438 828 572 455 428
BstBI TT'CG_AA
Cuts at: 110 4987 110
Size: 4877 1749
BstEII G'GTnAC_C
Cuts at: 2236 2236
Size: 6626
BstYI r'GATC_y
Cuts at: 1 1691 3651 4118 4141 4476 4722 5734 5820
Size: 1690 1960 467 23 335 246 1012 86
Cuts at: 5820 5832 6178 1
Size: 12 346 449
Fragments arranged by size:
1960 1690 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1291 3071 3463 1291
Size: 1780 392 4454
Fragments arranged by size:
4454 1780 392
BtgI C'CryG_G
Cuts at: 125 951 1555 2492 2503 125
Size: 826 604 937 11 4248
Fragments arranged by size:
4248 937 826 604 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1053 1720 2916 4576 4757 4920 1053
Size: 667 1196 1660 181 163 2759
Fragments arranged by size:
2759 1660 1196 667 181 163
BtsI GCAGTG_nn'
Cuts at: 3869 3869
Size: 6626
DraI TTT'AAA
Cuts at: 3625 3625
Size: 6626
DraIII CAC_nnn'GTG
Cuts at: 201 1804 3489 201
Size: 1603 1685 3338
Fragments arranged by size:
3338 1685 1603
DrdI GACnn_nn'nnGTC
Cuts at: 4332 5190 4332
Size: 858 5768
EaeI y'GGCC_r
Cuts at: 186 885 969 1229 1304 1388 1406 1415 1454
Size: 699 84 260 75 84 18 9 39
Cuts at: 1454 1596 1616 1653 1880 2802 2822 2840 2957
Size: 142 20 37 227 922 20 18 117
Cuts at: 2957 3164 3266 3406 3456 4211 4385 4776 4803
Size: 207 102 140 50 755 174 391 27
Cuts at: 4803 6148 186
Size: 1345 664
Fragments arranged by size:
1345 922 755 699 664 391 260 227
207 174 142 140 117 102 84 84
75 50 39 37 27 20 20 18
18 9
EagI C'GGCC_G
Cuts at: 186 969 1229 1304 1415 1616 4211 186
Size: 783 260 75 111 201 2595 2601
Fragments arranged by size:
2601 2595 783 260 201 111 75
EarI CTCTTCn'nnn_
Cuts at: 1961 2682 4649 4859 1961
Size: 721 1967 210 3728
Fragments arranged by size:
3728 1967 721 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1258 1833 4834 5144 5290 6436 1258
Size: 575 3001 310 146 1146 1448
Fragments arranged by size:
3001 1448 1146 575 310 146
EcoICRI GAG'CTC
Cuts at: 266 1986 3638 266
Size: 1720 1652 3254
Fragments arranged by size:
3254 1720 1652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 712 879 934 1261 1974 2883 3542 4451
Size: 336 167 55 327 713 909 659 909
Cuts at: 4451 4883 5630 5898 376
Size: 432 747 268 1104
Fragments arranged by size:
1104 909 909 747 713 659 432 336
327 268 167 55
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1931 3343 1126
Size: 805 1412 4409
Fragments arranged by size:
4409 1412 805
EcoO109I rG'GnC_Cy
Cuts at: 166 453 515 540 551 687 808 1249 1250
Size: 287 62 25 11 136 121 441 1
Cuts at: 1250 1436 1549 1550 1581 2445 2575 2743 2833
Size: 186 113 1 31 864 130 168 90
Cuts at: 2833 2834 2988 3467 3765 166
Size: 1 154 479 298 3027
Fragments arranged by size:
3027 864 479 441 298 287 186 168
154 136 130 121 113 90 62 31
25 11 1 1 1
EcoRI G'AATT_C
Cuts at: 3630 3630
Size: 6626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1287 2693 4645 1287
Size: 1406 1952 3268
Fragments arranged by size:
3268 1952 1406
FseI GG_CCGG'CC
Cuts at: 188 188
Size: 6626
FspI TGC'GCA
Cuts at: 286 4407 286
Size: 4121 2505
HaeII r_GCGC'y
Cuts at: 427 488 536 1453 1919 2302 2764 2912 3107
Size: 61 48 917 466 383 462 148 195
Cuts at: 3107 3232 3288 4060 4308 5330 6309 427
Size: 125 56 772 248 1022 979 744
Fragments arranged by size:
1022 979 917 772 744 466 462 383
248 195 148 125 61 56 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1597 1629 1804 1836 2011 2043 3341 3373 3404
Size: 32 175 32 175 32 1298 32 31
Cuts at: 3404 3436 3582 3614 4468 4498 4500 4530 1597
Size: 32 146 32 854 30 2 30 3693
Fragments arranged by size:
3693 1298 854 175 175 146 32 32
32 32 32 32 31 30 30 2
HincII GTy'rAC
Cuts at: 1365 2342 3665 1365
Size: 977 1323 4326
Fragments arranged by size:
4326 1323 977
KasI G'GCGC_C
Cuts at: 423 532 1915 2760 3228 4056 4304 423
Size: 109 1383 845 468 828 248 2745
Fragments arranged by size:
2745 1383 845 828 468 248 109
KpnI G_GTAC'C
Cuts at: 3584 3646 3584
Size: 62 6564
MluI A'CGCG_T
Cuts at: 1728 6059 1728
Size: 4331 2295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3353 5297 5481 3353
Size: 1944 184 4498
Fragments arranged by size:
4498 1944 184
MscI TGG'CCA
Cuts at: 887 1390 2842 3166 4387 887
Size: 503 1452 324 1221 3126
Fragments arranged by size:
3126 1452 1221 503 324
MslI CAynn'nnrTG
Cuts at: 2641 4615 4742 2641
Size: 1974 127 4525
Fragments arranged by size:
4525 1974 127
MspA1I CmG'CkG
Cuts at: 429 569 643 818 1386 1557 1790 1855 1952
Size: 140 74 175 568 171 233 65 97
Cuts at: 1952 2483 2998 3362 3737 4051 4411 5424 5669
Size: 531 515 364 375 314 360 1013 245
Cuts at: 5669 429
Size: 1386
Fragments arranged by size:
1386 1013 568 531 515 375 364 360
314 245 233 175 171 140 97 74
65
NaeI GCC'GGC
Cuts at: 186 1113 1308 1415 1596 4807 186
Size: 927 195 107 181 3211 2005
Fragments arranged by size:
3211 2005 927 195 181 107
NarI GG'CG_CC
Cuts at: 424 533 1916 2761 3229 4057 4305 424
Size: 109 1383 845 468 828 248 2745
Fragments arranged by size:
2745 1383 845 828 468 248 109
NcoI C'CATG_G
Cuts at: 125 125
Size: 6626
NgoMIV G'CCGG_C
Cuts at: 184 1111 1306 1413 1594 4805 184
Size: 927 195 107 181 3211 2005
Fragments arranged by size:
3211 2005 927 195 181 107
NheI G'CTAG_C
Cuts at: 2707 2860 2707
Size: 153 6473
NspI r_CATG'y
Cuts at: 285 620 2715 3477 3679 4179 5086 285
Size: 335 2095 762 202 500 907 1825
Fragments arranged by size:
2095 1825 907 762 500 335 202
PasI CC'CwG_GG
Cuts at: 2685 3367 2685
Size: 682 5944
PciI A'CATG_T
Cuts at: 5082 5082
Size: 6626
PflMI CCAn_nnn'nTGG
Cuts at: 201 201
Size: 6626
PfoI T'CCnGG_A
Cuts at: 1091 2557 1091
Size: 1466 5160
PmeI GTTT'AAAC
Cuts at: 3625 3625
Size: 6626
PmlI CAC'GTG
Cuts at: 702 702
Size: 6626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 502 534 538 570 976 1008 5765 5797 502
Size: 32 4 32 406 32 4757 32 1331
Fragments arranged by size:
4757 1331 406 32 32 32 32 4
PpuMI rG'GwC_Cy
Cuts at: 540 687 1581 2445 2743 2988 540
Size: 147 894 864 298 245 4178
Fragments arranged by size:
4178 894 864 298 245 147
PshAI GACnn'nnGTC
Cuts at: 2003 2003
Size: 6626
PspOMI G'GGCC_C
Cuts at: 808 1249 1549 1784 2574 2833 3467 808
Size: 441 300 235 790 259 634 3967
Fragments arranged by size:
3967 790 634 441 300 259 235
PstI C_TGCA'G
Cuts at: 2247 2387 2422 3673 4358 2247
Size: 140 35 1251 685 4515
Fragments arranged by size:
4515 1251 685 140 35
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6626
PvuII CAG'CTG
Cuts at: 1386 2483 4051 4411 1386
Size: 1097 1568 360 3601
Fragments arranged by size:
3601 1568 1097 360
RsrII CG'GwC_CG
Cuts at: 4821 4821
Size: 6626
SacI G_AGCT'C
Cuts at: 268 1988 3640 268
Size: 1720 1652 3254
Fragments arranged by size:
3254 1720 1652
SacII CC_GC'GG
Cuts at: 1558 1558
Size: 6626
SalI G'TCGA_C
Cuts at: 2340 3663 2340
Size: 1323 5303
SanDI GG'GwC_CC
Cuts at: 1581 2988 1581
Size: 1407 5219
SapI GCTCTTCn'nnn_
Cuts at: 2682 4649 4859 2682
Size: 1967 210 4449
Fragments arranged by size:
4449 1967 210
SbfI CC_TGCA'GG
Cuts at: 2387 3673 2387
Size: 1286 5340
ScaI AGT'ACT
Cuts at: 6143 6143
Size: 6626
SexAI A'CCwGG_T
Cuts at: 904 904
Size: 6626
SfcI C'TryA_G
Cuts at: 33 2243 2383 2418 3669 4354 5347 5538 33
Size: 2210 140 35 1251 685 993 191 1121
Fragments arranged by size:
2210 1251 1121 993 685 191 140 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 523 559 1414 3205 523
Size: 36 855 1791 3944
Fragments arranged by size:
3944 1791 855 36
SfoI GGC'GCC
Cuts at: 425 534 1917 2762 3230 4058 4306 425
Size: 109 1383 845 468 828 248 2745
Fragments arranged by size:
2745 1383 845 828 468 248 109
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6626
SgrAI Cr'CCGG_yG
Cuts at: 2041 2041
Size: 6626
SmaI CCC'GGG
Cuts at: 626 861 1868 2885 2938 3648 626
Size: 235 1007 1017 53 710 3604
Fragments arranged by size:
3604 1017 1007 710 235 53
SmlI C'TyrA_G
Cuts at: 997 1426 3781 4696 5188 5450 997
Size: 429 2355 915 492 262 2173
Fragments arranged by size:
2355 2173 915 492 429 262
SnaBI TAC'GTA
Cuts at: 3618 3618
Size: 6626
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6626
SphI G_CATG'C
Cuts at: 620 2715 3679 4179 620
Size: 2095 964 500 3067
Fragments arranged by size:
3067 2095 964 500
SrfI GCCC'GGGC
Cuts at: 2938 2938
Size: 6626
StuI AGG'CCT
Cuts at: 826 892 1378 826
Size: 66 486 6074
Fragments arranged by size:
6074 486 66
StyI C'CwwG_G
Cuts at: 125 635 2051 3760 4114 125
Size: 510 1416 1709 354 2637
Fragments arranged by size:
2637 1709 1416 510 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1996 4209 4342 4801 5010 421
Size: 1575 2213 133 459 209 2037
Fragments arranged by size:
2213 2037 1575 459 209 133
TatI w'GTAC_w
Cuts at: 6141 6141
Size: 6626
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 322 354 856 888 925 957 1328 1360 6143
Size: 32 502 32 37 32 371 32 4783
Cuts at: 6143 6175 322
Size: 32 773
Fragments arranged by size:
4783 773 502 371 37 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1360 4423 1360
Size: 3063 3563
XbaI T'CTAG_A
Cuts at: 2107 3657 2107
Size: 1550 5076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 202 1056 1083 2812 4121 202
Size: 854 27 1729 1309 2707
Fragments arranged by size:
2707 1729 1309 854 27
XmaI C'CCGG_G
Cuts at: 624 859 1866 2883 2936 3646 624
Size: 235 1007 1017 53 710 3604
Fragments arranged by size:
3604 1017 1007 710 235 53
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI AvrII BfrBI BsiWI
BsrGI BstXI BstZ17I ClaI CspCI EcoRV FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PsiI
PspXI PsrI SspI SwaI XhoI XmnI ZraI