Restriction map for pF1KSDA0174
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BbeI        BbrPI       
BcgI        BciVI       BglI        BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraIII      EaeI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
HphI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          300  GTCTAC               GT^[AC][GT]AC
AcsI           39  GAATTT               [GA]^AATT[CT]
AflII          30  CTTAAG               C^TTAAG
AlwNI        1028  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        26  AGCGCT               AGC^GCT
ApoI           39  GAATTT               [GA]^AATT[CT]
AseI          856  ATTAAT               AT^TAAT
AsnI          856  ATTAAT               AT^TAAT
BamHI         158  GGATCC               G^GATCC
BbvCI        1025  CCTCAGC              CCTCAGC(-5/-2)
BclI          363  TGATCA               T^GATCA
BfaI          274  CTAG                 C^TAG
BfrI           30  CTTAAG               C^TTAAG
BglII         579  AGATCT               A^GATCT
BlpI           22  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       22  GCTGAGC              GC^T[ACGT]AGC
BsgI          881  CTGCAC               GTGCAG(16/14)
BsmAI         574  GAGAC                GTCTC(1/5)
BssSI         193  CTCGTG               CACGAG(-5/-1)
BstF5I        805  CATCC                GGATG(2/0)
BstXI         473  CCAAAATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII          22  GCTGAGC              GC^T[ACGT]AGC
CfoI           27  GCGC                 GCG^C
DraI           16  TTTAAA               TTT^AAA
DraII         621  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          203  CCATGG               C^C[GA][CT]GG
Eco47III       26  AGCGCT               AGC^GCT
EcoO109I      621  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       203  CCATGG               C^C[AT][AT]GG
FbaI          363  TGATCA               T^GATCA
FokI          805  CATCC                GGATG(9/13)
HaeII          26  AGCGCT               [GA]GCGC^[CT]
HhaI           27  GCGC                 GCG^C
HinP1I         27  GCGC                 G^CGC
HindIII       925  AAGCTT               A^AGCTT
MaeI          274  CTAG                 C^TAG
MaeII         521  ACGT                 A^CGT
MaeIII        227  GTGAC                ^GT[ACGT]AC
NcoI          203  CCATGG               C^CATGG
PflMI         658  CCAGTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         326  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         856  ATTAAT               AT^TAAT
PstI          941  CTGCAG               CTGCA^G
SacI          152  GAGCTC               GAGCT^C
SapI         1072  GAAGAGC              GCTCTTC(1/4)
SmlI           30  CTTAAG               C^T[CT][GA]AG
SspI          799  AATATT               AAT^ATT
StuI          788  AGGCCT               AGG^CCT
StyI          203  CCATGG               C^C[AT][AT]GG
TaqI          324  TCGA                 T^CGA
Tsp45I        227  GTGAC                ^GT[GC]AC
TspRI         737  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       324  TCGA                 T^CGA
Van91I        658  CCAGTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          222  GTAC                 GT^AC
              523  GTAC                
AspHI         152  GAGCTC               G[AT]GC[AT]^C
              169  GAGCAC              
BanI          681  GGTGCC               G^G[CT][GA]CC
             1015  GGTGCC              
BanII           6  GGGCTC               G[GA]GC[CT]^C
              152  GAGCTC              
BbsI          184  GAAGAC               GAAGAC(2/6)
             1036  GAAGAC              
BcnI          180  CCGGG                CC^[GC]GG
             1061  CCCGG               
BpmI          328  CTCCAG               CTGGAG(16/14)
              214  CTGGAG              
BpuAI         184  GAAGAC               GAAGAC(2/6)
             1036  GAAGAC              
BsiHKAI       152  GAGCTC               G[AT]GC[AT]^C
              169  GAGCAC              
BsmFI         747  GGGAC                GGGAC(10/14)
              778  GGGAC               
BspMI         230  ACCTGC               ACCTGC(4/8)
              939  ACCTGC              
DrdI          538  GACTCTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              955  GACAACTTTGTC        
EcoNI         192  CCTCGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              625  CCTGGAAGAGG         
HapII         180  CCGG                 C^CGG
             1062  CCGG                
HpaII         180  CCGG                 C^CGG
             1062  CCGG                
MslI          693  CATGCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              699  CATGCCCATG          
MspI          180  CCGG                 C^CGG
             1062  CCGG                
MwoI           97  GCCCAGAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              978  GCCATCTGTGC         
NciI          180  CCGGG                CC^[GC]GG
             1061  CCCGG               
PleI          326  GACTC                GAGTC(4/5)
              538  GACTC               
RsaI          222  GTAC                 GT^AC
              523  GTAC                
SfaNI        1006  GCATC                GCATC(5/9)
             1030  GCATC               
SfcI          845  CTGTAG               C^T[GA][CT]AG
              941  CTGCAG              
XcmI          203  CCATGGAGATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              473  CCAAAATCCTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AluI        AlwI        AvaII       BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      HaeIII      HinfI       ItaI        Ksp632I     
MboI        MboII       MflI        MnlI        MseI        
MvaI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       TfiI        Tru9I       TseI        
Tsp509I     XhoII       
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