Restriction map for pF1KSDA0167
 (Linear) (Six-base) MAPSORT of: KIAA0167.seq  Check: 9062  from: 1  to: 2508



KIAA0167.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1385    2508 
   Size:       1385    1123
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411    1402    1544    1565    2508 
   Size:        411     991     142      21     943

  Fragments arranged by size: 

                 991     943     411     142      21
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     751    2508 
   Size:        751    1757
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     412     717     883    2055    2508 
   Size:        291     121     305     166    1172     453

  Fragments arranged by size: 

                1172     453     305     291     166     121
 
ApaI G_GGCC'C

Cuts at:      0    1785    2508 
   Size:       1785     723
 
ApoI r'AATT_y

Cuts at:      0    1395    1758    2508 
   Size:       1395     363     750

  Fragments arranged by size: 

                1395     750     363
 
AvaI C'yCGr_G

Cuts at:      0     563    1197    1212    1403    2376    2508 
   Size:        563     634      15     191     973     132

  Fragments arranged by size: 

                 973     634     563     191     132      15
 
BanI G'GyrC_C

Cuts at:      0     378    1816    2064    2508 
   Size:        378    1438     248     444

  Fragments arranged by size: 

                1438     444     378     248
 
BanII G_rGCy'C

Cuts at:      0     594     650     860    1259    1410    1447    1785    2234 
   Size:        594      56     210     399     151      37     338     449

Cuts at:   2234    2508 
   Size:        274

  Fragments arranged by size: 

                 594     449     399     338     274     210     151      56
                  37
 
BbeI G_GCGC'C

Cuts at:      0    1820    2068    2508 
   Size:       1820     248     440

  Fragments arranged by size: 

                1820     440     248
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     304     417     657    2508 
   Size:        304     113     240    1851

  Fragments arranged by size: 

                1851     304     240     113
 
BbvCI CC'TCA_GC

Cuts at:      0    1239    1411    2508 
   Size:       1239     172    1097

  Fragments arranged by size: 

                1239    1097     172
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     856     890    2508 
   Size:        856      34    1618

  Fragments arranged by size: 

                1618     856      34
 
BclI T'GATC_A

Cuts at:      0     167    2508 
   Size:        167    2341
 
BfrBI ATG'CAT

Cuts at:      0       3    2508 
   Size:          3    2505
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70    1735    2119    2329    2508 
   Size:         70    1665     384     210     179

  Fragments arranged by size: 

                1665     384     210     179      70
 
BlpI GC'TnA_GC

Cuts at:      0    2071    2508 
   Size:       2071     437
 
Bme1580I G_kGCm'C

Cuts at:      0     381    1785    1864    2508 
   Size:        381    1404      79     644

  Fragments arranged by size: 

                1404     644     381      79
 
BmgBI CAC'GTC

Cuts at:      0    1592    2130    2241    2280    2508 
   Size:       1592     538     111      39     228

  Fragments arranged by size: 

                1592     538     228     111      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1121    1331    2508 
   Size:       1121     210    1177

  Fragments arranged by size: 

                1177    1121     210
 
BmtI G_CTAG'C

Cuts at:      0    2474    2508 
   Size:       2474      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     313     454    1454    2347    2508 
   Size:        106     207     141    1000     893     161

  Fragments arranged by size: 

                1000     893     207     161     141     106
 
Bpu10I CC'TnA_GC

Cuts at:      0    1239    1411    1441    2508 
   Size:       1239     172      30    1067

  Fragments arranged by size: 

                1239    1067     172      30
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1430    2508 
   Size:       1430    1078
 
BsaAI yAC'GTr

Cuts at:      0    1796    2508 
   Size:       1796     712
 
BsaHI Gr'CG_yC

Cuts at:      0    1817    2065    2294    2489    2508 
   Size:       1817     248     229     195      19

  Fragments arranged by size: 

                1817     248     229     195      19
 
BsaWI w'CCGG_w

Cuts at:      0     771    1391    1742    2508 
   Size:        771     620     351     766

  Fragments arranged by size: 

                 771     766     620     351
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2008    2038    2508 
   Size:       2008      30     470

  Fragments arranged by size: 

                2008     470      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     521    1506    1633    2029    2098    2508 
   Size:        521     985     127     396      69     410

  Fragments arranged by size: 

                 985     521     410     396     127      69
 
BseYI C'CCAG_C

Cuts at:      0     225     373     401     739    1330    1534    2091    2407 
   Size:        225     148      28     338     591     204     557     316

Cuts at:   2407    2425    2443    2508 
   Size:         18      18      65

  Fragments arranged by size: 

                 591     557     338     316     225     204     148      65
                  28      18      18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     892    2137    2508 
   Size:        892    1245     371

  Fragments arranged by size: 

                1245     892     371
 
BsiEI CG_ry'CG

Cuts at:      0    2228    2508 
   Size:       2228     280
 
BsiHKAI G_wGCw'C

Cuts at:      0     594     860     895    1276    2077    2234    2508 
   Size:        594     266      35     381     801     157     274

  Fragments arranged by size: 

                 801     594     381     274     266     157      35
 
BsmI GAATG_Cn'

Cuts at:      0     152    2508 
   Size:        152    2356
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     472    2508 
   Size:        472    2036
 
Bsp1286I G_dGCh'C

Cuts at:      0     381     594     650     860     895    1259    1276    1410 
   Size:        381     213      56     210      35     364      17     134

Cuts at:   1410    1447    1785    1864    2077    2234    2508 
   Size:         37     338      79     213     157     274

  Fragments arranged by size: 

                 381     364     338     274     213     213     210     157
                 134      79      56      37      35      17
 
BspEI T'CCGG_A

Cuts at:      0    1742    2508 
   Size:       1742     766
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1385    2508 
   Size:       1385    1123
 
BsrBI CCG'CTC

Cuts at:      0     197    1623    2175    2508 
   Size:        197    1426     552     333

  Fragments arranged by size: 

                1426     552     333     197
 
BsrDI GCAATG_nn'

Cuts at:      0    1678    2508 
   Size:       1678     830
 
BssSI C'ACGA_G

Cuts at:      0     581    2508 
   Size:        581    1927
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     385    2259    2508 
   Size:        385    1874     249

  Fragments arranged by size: 

                1874     385     249
 
BstEII G'GTnAC_C

Cuts at:      0     690     847    2508 
   Size:        690     157    1661

  Fragments arranged by size: 

                1661     690     157
 
BstYI r'GATC_y

Cuts at:      0    1655    2508 
   Size:       1655     853
 
Bsu36I CC'TnA_GG

Cuts at:      0      85    2508 
   Size:         85    2423
 
BtgI C'CryG_G

Cuts at:      0     471    1155    1475    1724    1906    2508 
   Size:        471     684     320     249     182     602

  Fragments arranged by size: 

                 684     602     471     320     249     182
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     236     245    2508 
   Size:        236       9    2263

  Fragments arranged by size: 

                2263     236       9
 
BtsI GCAGTG_nn'

Cuts at:      0      38     884    2508 
   Size:         38     846    1624

  Fragments arranged by size: 

                1624     846      38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1751    1786    2508 
   Size:       1751      35     722

  Fragments arranged by size: 

                1751     722      35
 
EaeI y'GGCC_r

Cuts at:      0      62     780    1727    1903    1949    2225    2396    2414 
   Size:         62     718     947     176      46     276     171      18

Cuts at:   2414    2508 
   Size:         94

  Fragments arranged by size: 

                 947     718     276     176     171      94      62      46
                  18
 
EagI C'GGCC_G

Cuts at:      0    2225    2508 
   Size:       2225     283
 
EarI CTCTTCn'nnn_

Cuts at:      0     822    1541    2010    2508 
   Size:        822     719     469     498

  Fragments arranged by size: 

                 822     719     498     469
 
EcoICRI GAG'CTC

Cuts at:      0     592     858    2232    2508 
   Size:        592     266    1374     276

  Fragments arranged by size: 

                1374     592     276     266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     313     411     454    1402    1454    1544    1565 
   Size:        106     207      98      43     948      52      90      21

Cuts at:   1565    2347    2508 
   Size:        782     161

  Fragments arranged by size: 

                 948     782     207     161     106      98      90      52
                  43      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     902    1781    1782    2193    2508 
   Size:        902     879       1     411     315

  Fragments arranged by size: 

                 902     879     411     315       1
 
EcoRI G'AATT_C

Cuts at:      0    1758    2508 
   Size:       1758     750
 
FspI TGC'GCA

Cuts at:      0     700    1702    2508 
   Size:        700    1002     806

  Fragments arranged by size: 

                1002     806     700
 
HaeII r_GCGC'y

Cuts at:      0    1820    2068    2314    2468    2508 
   Size:       1820     248     246     154      40

  Fragments arranged by size: 

                1820     248     246     154      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29      61    1351    1383    2008    2040    2508 
   Size:         29      32    1290      32     625      32     468

  Fragments arranged by size: 

                1290     625     468      32      32      32      29
 
HindIII A'AGCT_T

Cuts at:      0     975    2508 
   Size:        975    1533
 
KasI G'GCGC_C

Cuts at:      0    1816    2064    2508 
   Size:       1816     248     444

  Fragments arranged by size: 

                1816     444     248
 
MscI TGG'CCA

Cuts at:      0      64     782    1729    1905    2508 
   Size:         64     718     947     176     603

  Fragments arranged by size: 

                 947     718     603     176      64
 
MspA1I CmG'CkG

Cuts at:      0     480     760     874    2070    2091    2103    2202    2343 
   Size:        480     280     114    1196      21      12      99     141

Cuts at:   2343    2508 
   Size:        165

  Fragments arranged by size: 

                1196     480     280     165     141     114      99      21
                  12
 
NarI GG'CG_CC

Cuts at:      0    1817    2065    2508 
   Size:       1817     248     443

  Fragments arranged by size: 

                1817     443     248
 
NcoI C'CATG_G

Cuts at:      0     471    1475    2508 
   Size:        471    1004    1033

  Fragments arranged by size: 

                1033    1004     471
 
NheI G'CTAG_C

Cuts at:      0    2470    2508 
   Size:       2470      38
 
NsiI A_TGCA'T

Cuts at:      0       5    2508 
   Size:          5    2503
 
NspI r_CATG'y

Cuts at:      0       7    2508 
   Size:          7    2501
 
PasI CC'CwG_GG

Cuts at:      0     898    2298    2508 
   Size:        898    1400     210

  Fragments arranged by size: 

                1400     898     210
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     291     675     687    2130    2508 
   Size:        291     384      12    1443     378

  Fragments arranged by size: 

                1443     384     378     291      12
 
PfoI T'CCnGG_A

Cuts at:      0     122     668    2508 
   Size:        122     546    1840

  Fragments arranged by size: 

                1840     546     122
 
PmlI CAC'GTG

Cuts at:      0    1796    2508 
   Size:       1796     712
 
PpuMI rG'GwC_Cy

Cuts at:      0     902    2193    2508 
   Size:        902    1291     315

  Fragments arranged by size: 

                1291     902     315
 
PspOMI G'GGCC_C

Cuts at:      0    1781    2508 
   Size:       1781     727
 
PstI C_TGCA'G

Cuts at:      0      33     619     879    1349    2508 
   Size:         33     586     260     470    1159

  Fragments arranged by size: 

                1159     586     470     260      33
 
PvuII CAG'CTG

Cuts at:      0     480     760     874    2103    2202    2343    2508 
   Size:        480     280     114    1229      99     141     165

  Fragments arranged by size: 

                1229     480     280     165     141     114      99
 
SacI G_AGCT'C

Cuts at:      0     594     860    2234    2508 
   Size:        594     266    1374     274

  Fragments arranged by size: 

                1374     594     274     266
 
SanDI GG'GwC_CC

Cuts at:      0     902    2508 
   Size:        902    1606
 
SexAI A'CCwGG_T

Cuts at:      0     284    2508 
   Size:        284    2224
 
SfcI C'TryA_G

Cuts at:      0      29     615     875    1345    2508 
   Size:         29     586     260     470    1163

  Fragments arranged by size: 

                1163     586     470     260      29
 
SfoI GGC'GCC

Cuts at:      0    1818    2066    2508 
   Size:       1818     248     442

  Fragments arranged by size: 

                1818     442     248
 
SmaI CCC'GGG

Cuts at:      0    1199    2378    2508 
   Size:       1199    1179     130

  Fragments arranged by size: 

                1199    1179     130
 
SphI G_CATG'C

Cuts at:      0       7    2508 
   Size:          7    2501
 
StuI AGG'CCT

Cuts at:      0     139     512    1295    2508 
   Size:        139     373     783    1213

  Fragments arranged by size: 

                1213     783     373     139
 
StyI C'CwwG_G

Cuts at:      0     471     518    1475    1751    2508 
   Size:        471      47     957     276     757

  Fragments arranged by size: 

                 957     757     471     276      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208    1276    2508 
   Size:        208    1068    1232

  Fragments arranged by size: 

                1232    1068     208
 
TatI w'GTAC_w

Cuts at:      0     314    2508 
   Size:        314    2194
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1809    2508 
   Size:       1809     699
 
XmaI C'CCGG_G

Cuts at:      0    1197    2376    2508 
   Size:       1197    1179     132

  Fragments arranged by size: 

                1197    1179     132
 
XmnI GAAnn'nnTTC

Cuts at:      0     448    2508 
   Size:        448    2060
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FspI    HaeII    Hin4I  HindIII     KasI     MscI   MspA1I     NarI
     NcoI     NheI     NsiI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI    SexAI     SfcI
     SfoI     SmaI     SphI     StuI     StyI    TaqII     TatI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI    BglII     BplI    BpuEI    BsaBI    BsiWI
    BspHI    BsrFI    BsrGI   BssHII    BstBI    BstXI  BstZ17I     ClaI
     DraI   DraIII     DrdI     EciI    EcoNI    EcoRV     FalI     FseI
    FspAI   HincII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
     NaeI     NdeI   NgoMIV     NotI     NruI     PacI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I     XbaI
     XhoI     ZraI
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