Restriction map for pF1KSDA0132
 (Linear) (Six-base) MAPSORT of: KIAA0132.seq  Check: 2888  from: 1  to: 1872



KIAA0132.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     933    1065    1872 
   Size:        933     132     807

  Fragments arranged by size: 

                 933     807     132
 
AatII G_ACGT'C

Cuts at:      0     236    1872 
   Size:        236    1636
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138    1872 
   Size:        138    1734
 
AfeI AGC'GCT

Cuts at:      0    1606    1872 
   Size:       1606     266
 
AflIII A'CryG_T

Cuts at:      0    1724    1872 
   Size:       1724     148
 
AleI CACnn'nnGTG

Cuts at:      0     602     943    1313    1872 
   Size:        602     341     370     559

  Fragments arranged by size: 

                 602     559     370     341
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     620    1551    1872 
   Size:        620     931     321

  Fragments arranged by size: 

                 931     620     321
 
AlwNI CAG_nnn'CTG

Cuts at:      0     225     534     798    1872 
   Size:        225     309     264    1074

  Fragments arranged by size: 

                1074     309     264     225
 
ApoI r'AATT_y

Cuts at:      0     412    1872 
   Size:        412    1460
 
AvaI C'yCGr_G

Cuts at:      0     608    1872 
   Size:        608    1264
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1536    1569    1872 
   Size:       1536      33     303

  Fragments arranged by size: 

                1536     303      33
 
BanI G'GyrC_C

Cuts at:      0     960    1048    1077    1872 
   Size:        960      88      29     795

  Fragments arranged by size: 

                 960     795      88      29
 
BanII G_rGCy'C

Cuts at:      0     923    1835    1872 
   Size:        923     912      37

  Fragments arranged by size: 

                 923     912      37
 
BbeI G_GCGC'C

Cuts at:      0     964    1872 
   Size:        964     908
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     309     726    1872 
   Size:        309     417    1146

  Fragments arranged by size: 

                1146     417     309
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     534     568     822     856    1872 
   Size:        534      34     254      34    1016

  Fragments arranged by size: 

                1016     534     254      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     391    1872 
   Size:        391    1481
 
BclI T'GATC_A

Cuts at:      0    1496    1872 
   Size:       1496     376
 
BfrBI ATG'CAT

Cuts at:      0     627    1872 
   Size:        627    1245
 
BglI GCCn_nnn'nGGC

Cuts at:      0     424    1097    1872 
   Size:        424     673     775

  Fragments arranged by size: 

                 775     673     424
 
BglII A'GATC_T

Cuts at:      0     908    1872 
   Size:        908     964
 
BlpI GC'TnA_GC

Cuts at:      0     214     572    1015    1872 
   Size:        214     358     443     857

  Fragments arranged by size: 

                 857     443     358     214
 
Bme1580I G_kGCm'C

Cuts at:      0      70     609    1235    1668    1872 
   Size:         70     539     626     433     204

  Fragments arranged by size: 

                 626     539     433     204      70
 
BmgBI CAC'GTC

Cuts at:      0     462    1628    1872 
   Size:        462    1166     244

  Fragments arranged by size: 

                1166     462     244
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     267     762    1034    1872 
   Size:        267     495     272     838

  Fragments arranged by size: 

                 838     495     272     267
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1045    1152    1791    1872 
   Size:        187     858     107     639      81

  Fragments arranged by size: 

                 858     639     187     107      81
 
BsaHI Gr'CG_yC

Cuts at:      0     127     233     829     961    1532    1872 
   Size:        127     106     596     132     571     340

  Fragments arranged by size: 

                 596     571     340     132     127     106
 
BsaWI w'CCGG_w

Cuts at:      0    1058    1872 
   Size:       1058     814
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     352     382     412     442    1150    1180    1872 
   Size:        352      30      30      30     708      30     692

  Fragments arranged by size: 

                 708     692     352      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     667     931    1872 
   Size:        667     264     941

  Fragments arranged by size: 

                 941     667     264
 
BseYI C'CCAG_C

Cuts at:      0      28     134     541    1564    1872 
   Size:         28     106     407    1023     308

  Fragments arranged by size: 

                1023     407     308     106      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547     913    1528    1872 
   Size:        547     366     615     344

  Fragments arranged by size: 

                 615     547     366     344
 
BsiEI CG_ry'CG

Cuts at:      0     269    1872 
   Size:        269    1603
 
BsiHKAI G_wGCw'C

Cuts at:      0     923    1872 
   Size:        923     949
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1618    1872 
   Size:       1618     254
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     609     923    1235    1668    1835    1872 
   Size:         70     539     314     312     433     167      37

  Fragments arranged by size: 

                 539     433     314     312     167      70      37
 
BspHI T'CATG_A

Cuts at:      0     197     464    1872 
   Size:        197     267    1408

  Fragments arranged by size: 

                1408     267     197
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     234     933    1065    1079    1872 
   Size:        234     699     132      14     793

  Fragments arranged by size: 

                 793     699     234     132      14
 
BsrBI CCG'CTC

Cuts at:      0    1088    1338    1872 
   Size:       1088     250     534

  Fragments arranged by size: 

                1088     534     250
 
BsrDI GCAATG_nn'

Cuts at:      0     552    1511    1872 
   Size:        552     959     361

  Fragments arranged by size: 

                 959     552     361
 
BsrFI r'CCGG_y

Cuts at:      0     264    1096    1655    1872 
   Size:        264     832     559     217

  Fragments arranged by size: 

                 832     559     264     217
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     948    1872 
   Size:        948     924
 
BstEII G'GTnAC_C

Cuts at:      0     684    1779    1872 
   Size:        684    1095      93

  Fragments arranged by size: 

                1095     684      93
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     438    1176    1872 
   Size:        396      42     738     696

  Fragments arranged by size: 

                 738     696     396      42
 
BstYI r'GATC_y

Cuts at:      0       9     866     908    1872 
   Size:          9     857      42     964

  Fragments arranged by size: 

                 964     857      42       9
 
Bsu36I CC'TnA_GG

Cuts at:      0      71    1872 
   Size:         71    1801
 
BtgI C'CryG_G

Cuts at:      0     437     989    1081    1127    1293    1421    1826    1872 
   Size:        437     552      92      46     166     128     405      46

  Fragments arranged by size: 

                 552     437     405     166     128      92      46      46
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     543    1872 
   Size:        543    1329
 
BtsI GCAGTG_nn'

Cuts at:      0     238     519     672    1872 
   Size:        238     281     153    1200

  Fragments arranged by size: 

                1200     281     238     153
 
ClaI AT'CG_AT

Cuts at:      0    1262    1872 
   Size:       1262     610
 
DraIII CAC_nnn'GTG

Cuts at:      0     110     291    1356    1872 
   Size:        110     181    1065     516

  Fragments arranged by size: 

                1065     516     181     110
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     499     901    1872 
   Size:        499     402     971

  Fragments arranged by size: 

                 971     499     402
 
EaeI y'GGCC_r

Cuts at:      0     266     641    1094    1266    1872 
   Size:        266     375     453     172     606

  Fragments arranged by size: 

                 606     453     375     266     172
 
EagI C'GGCC_G

Cuts at:      0     266    1872 
   Size:        266    1606
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     133    1081    1872 
   Size:        133     948     791

  Fragments arranged by size: 

                 948     791     133
 
EcoICRI GAG'CTC

Cuts at:      0     921    1872 
   Size:        921     951
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     187    1045    1152    1791    1872 
   Size:        138      49     858     107     639      81

  Fragments arranged by size: 

                 858     639     138     107      81      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0      32    1872 
   Size:         32    1840
 
EcoRI G'AATT_C

Cuts at:      0     412    1872 
   Size:        412    1460
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     204    1872 
   Size:        204    1668
 
HaeII r_GCGC'y

Cuts at:      0     405     476     799     964    1175    1608    1872 
   Size:        405      71     323     165     211     433     264

  Fragments arranged by size: 

                 433     405     323     264     211     165      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     352     384     998    1030    1872 
   Size:        352      32     614      32     842

  Fragments arranged by size: 

                 842     614     352      32      32
 
HincII GTy'rAC

Cuts at:      0     827    1872 
   Size:        827    1045
 
KasI G'GCGC_C

Cuts at:      0     960    1872 
   Size:        960     912
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     902    1029    1872 
   Size:        902     127     843

  Fragments arranged by size: 

                 902     843     127
 
MscI TGG'CCA

Cuts at:      0     643    1268    1872 
   Size:        643     625     604

  Fragments arranged by size: 

                 643     625     604
 
MslI CAynn'nnrTG

Cuts at:      0     361     436     602     622     943    1228    1249    1274 
   Size:        361      75     166      20     321     285      21      25

Cuts at:   1274    1313    1504    1872 
   Size:         39     191     368

  Fragments arranged by size: 

                 368     361     321     285     191     166      75      39
                  25      21      20
 
MspA1I CmG'CkG

Cuts at:      0     225     534     852     887     991    1083    1459    1590 
   Size:        225     309     318      35     104      92     376     131

Cuts at:   1590    1872 
   Size:        282

  Fragments arranged by size: 

                 376     318     309     282     225     131     104      92
                  35
 
NaeI GCC'GGC

Cuts at:      0    1098    1872 
   Size:       1098     774
 
NarI GG'CG_CC

Cuts at:      0     961    1872 
   Size:        961     911
 
NcoI C'CATG_G

Cuts at:      0     437    1826    1872 
   Size:        437    1389      46

  Fragments arranged by size: 

                1389     437      46
 
NgoMIV G'CCGG_C

Cuts at:      0    1096    1872 
   Size:       1096     776
 
NsiI A_TGCA'T

Cuts at:      0     629    1872 
   Size:        629    1243
 
NspI r_CATG'y

Cuts at:      0     627    1872 
   Size:        627    1245
 
PasI CC'CwG_GG

Cuts at:      0    1668    1872 
   Size:       1668     204
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     279     291    1205    1872 
   Size:        279      12     914     667

  Fragments arranged by size: 

                 914     667     279      12
 
PfoI T'CCnGG_A

Cuts at:      0    1730    1872 
   Size:       1730     142
 
PstI C_TGCA'G

Cuts at:      0      59     245     514     845     857     875    1076    1872 
   Size:         59     186     269     331      12      18     201     796

  Fragments arranged by size: 

                 796     331     269     201     186      59      18      12
            
 
PvuII CAG'CTG

Cuts at:      0     225     534     852    1459    1590    1872 
   Size:        225     309     318     607     131     282

  Fragments arranged by size: 

                 607     318     309     282     225     131
 
SacI G_AGCT'C

Cuts at:      0     923    1872 
   Size:        923     949
 
SacII CC_GC'GG

Cuts at:      0     992    1084    1872 
   Size:        992      92     788

  Fragments arranged by size: 

                 992     788      92
 
SbfI CC_TGCA'GG

Cuts at:      0    1076    1872 
   Size:       1076     796
 
SexAI A'CCwGG_T

Cuts at:      0     899    1872 
   Size:        899     973
 
SfcI C'TryA_G

Cuts at:      0      55     241     510     841     853     871    1072    1872 
   Size:         55     186     269     331      12      18     201     800

  Fragments arranged by size: 

                 800     331     269     201     186      55      18      12
            
 
SfoI GGC'GCC

Cuts at:      0     962    1872 
   Size:        962     910
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1655    1872 
   Size:       1655     217
 
SmaI CCC'GGG

Cuts at:      0     610    1872 
   Size:        610    1262
 
StuI AGG'CCT

Cuts at:      0      18     187    1872 
   Size:         18     169    1685

  Fragments arranged by size: 

                1685     169      18
 
StyI C'CwwG_G

Cuts at:      0     389     437     965    1704    1826    1872 
   Size:        389      48     528     739     122      46

  Fragments arranged by size: 

                 739     528     389     122      48      46
 
TatI w'GTAC_w

Cuts at:      0     614    1872 
   Size:        614    1258
 
XmaI C'CCGG_G

Cuts at:      0     608    1872 
   Size:        608    1264
 
ZraI GAC'GTC

Cuts at:      0     234    1872 
   Size:        234    1638
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI
     ApoI     AvaI     BaeI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BpmI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PasI    PflMI     PfoI     PstI    PvuII     SacI
    SacII     SbfI    SexAI     SfcI     SfoI    SgrAI     SmaI     StuI
     StyI     TatI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII    BamHI    BbvCI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsiWI     BsmI
    BspEI    BsrGI   BssHII    BssSI    BstBI  BstZ17I    CspCI     DraI
     EarI    EcoNI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI     PacI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     SapI     ScaI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com