Restriction map for pF1KSDA0126
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglI        BlnI        BsaAI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspEI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KspI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NspV        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       2584  GGTACC               G^GTACC
AlwNI        1168  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2046  CCTGAGG              CC^T[ACGT]AGG
Asp718I      2584  GGTACC               G^GTACC
BalI         1814  TGGCCA               TGG^CCA
BamHI        3000  GGATCC               G^GATCC
BbsI          999  GAAGAC               GAAGAC(2/6)
BclI         1599  TGATCA               T^GATCA
BcnI         1048  CCGGG                CC^[GC]GG
BpuAI         999  GAAGAC               GAAGAC(2/6)
BsaI         2219  GAGACC               GGTCTC(1/5)
BsaWI        2132  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     1458  TGTACA               T^GTACA
BspHI         824  TCATGA               T^CATGA
BspLU11I     2282  ACATGT               A^CATGT
BsrBI         207  CCGCTC               CCGCTC(-3/-3)
BsrGI        1458  TGTACA               T^GTACA
BstAPI       1629  GCATCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2046  CCTGAGG              CC^T[ACGT]AGG
CfoI         2646  GCGC                 GCG^C
Eco81I       2046  CCTGAGG              CC^T[ACGT]AGG
EcoRI         178  GAATTC               G^AATTC
EcoRV        2761  GATATC               GAT^ATC
EcoT22I       893  ATGCAT               ATGCA^T
FbaI         1599  TGATCA               T^GATCA
HhaI         2646  GCGC                 GCG^C
HinP1I       2646  GCGC                 G^CGC
HincII        661  GTTGAC               GT[CT]^[GA]AC
HindII        661  GTTGAC               GT[CT]^[GA]AC
KpnI         2584  GGTACC               GGTAC^C
MluNI        1814  TGGCCA               TGG^CCA
MscI         1814  TGGCCA               TGG^CCA
NciI         1048  CCGGG                CC^[GC]GG
NheI          133  GCTAGC               G^CTAGC
NsiI          893  ATGCAT               ATGCA^T
PacI          451  TTAATTAA             TTAAT^TAA
PflMI        1262  CCAAGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         971  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1636  CTGCAG               CTGCA^G
RcaI          824  TCATGA               T^CATGA
SacI          871  GAGCTC               GAGCT^C
SexAI        2681  ACCTGGT              A^CC[AT]GGT
SphI         1242  GCATGC               GCATG^C
SspBI        1458  TGTACA               T^GTACA
Van91I       1262  CCAAGATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          867  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII       2139  ACGTGT               A^C[GA][CT]GT
             2282  ACATGT              
Asp700I       955  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2664  GAACTACTTC          
AspHI         871  GAGCTC               G[AT]GC[AT]^C
             3012  GAGCAC              
AvaII         972  GGACC                G^G[AT]CC
             3135  GGACC               
BanI         2584  GGTACC               G^G[CT][GA]CC
             2701  GGTGCC              
BciVI        2116  GTATCC               GTATCC(6/5)
             2265  GTATCC              
BlpI         1657  GCTCAGC              GC^T[ACGT]AGC
             2641  GCTGAGC             
Bpu1102I     1657  GCTCAGC              GC^T[ACGT]AGC
             2641  GCTGAGC             
BsaBI        1713  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2296  GATACCTATC          
BseRI        1390  CTCCTC               GAGGAG(10/8)
             2794  GAGGAG              
BsiHKAI       871  GAGCTC               G[AT]GC[AT]^C
             3012  GAGCAC              
CelII        1657  GCTCAGC              GC^T[ACGT]AGC
             2641  GCTGAGC             
DraII         407  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              971  AGGACCC             
DsaI         1790  CCATGG               C^C[GA][CT]GG
             3131  CCATGG              
EaeI         1814  TGGCCA               [CT]^GGCC[GA]
             3145  CGGCCA              
EcoO109I      407  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              971  AGGACCC             
MamI         1713  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2296  GATACCTATC          
NcoI         1790  CCATGG               C^CATGG
             3131  CCATGG              
SmlI           30  CTCAAG               C^T[CT][GA]AG
             1420  CTCAAG              
XcmI         2405  CCATCTTCCTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3050  CCAGAGTCACTGTGG     
XmnI          955  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2664  GAACTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BanII       BbvI        BfaI        BglII       
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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