Restriction map for pF1KSDA0126
 (Linear) (Six-base) MAPSORT of: KIAA0126.seq  Check: 3425  from: 1  to: 3219



KIAA0126.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1773    3219 
   Size:       1773    1446
 
Acc65I G'GTAC_C

Cuts at:      0    2584    3219 
   Size:       2584     635
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127    1195    1369    2706    3219 
   Size:        127    1068     174    1337     513

  Fragments arranged by size: 

                1337    1068     513     174     127
 
AflIII A'CryG_T

Cuts at:      0    2139    2282    3219 
   Size:       2139     143     937

  Fragments arranged by size: 

                2139     937     143
 
AleI CACnn'nnGTG

Cuts at:      0    3021    3219 
   Size:       3021     198
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     618    1265    3219 
   Size:        618     647    1954

  Fragments arranged by size: 

                1954     647     618
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1173    3219 
   Size:       1173    2046
 
ApoI r'AATT_y

Cuts at:      0     178    1897    2209    2343    2728    2799    3219 
   Size:        178    1719     312     134     385      71     420

  Fragments arranged by size: 

                1719     420     385     312     178     134      71
 
BamHI G'GATC_C

Cuts at:      0    3000    3219 
   Size:       3000     219
 
BanI G'GyrC_C

Cuts at:      0    2584    2701    3219 
   Size:       2584     117     518

  Fragments arranged by size: 

                2584     518     117
 
BanII G_rGCy'C

Cuts at:      0     720     875    1834    3219 
   Size:        720     155     959    1385

  Fragments arranged by size: 

                1385     959     720     155
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1006    3219 
   Size:       1006    2213
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2127    2276    3219 
   Size:       2127     149     943

  Fragments arranged by size: 

                2127     943     149
 
BclI T'GATC_A

Cuts at:      0    1599    3219 
   Size:       1599    1620
 
BfrBI ATG'CAT

Cuts at:      0     895    3219 
   Size:        895    2324
 
BglII A'GATC_T

Cuts at:      0     653     737     864    1160    3219 
   Size:        653      84     127     296    2059

  Fragments arranged by size: 

                2059     653     296     127      84
 
BlpI GC'TnA_GC

Cuts at:      0    1658    2642    3219 
   Size:       1658     984     577

  Fragments arranged by size: 

                1658     984     577
 
Bme1580I G_kGCm'C

Cuts at:      0    1419    2819    3219 
   Size:       1419    1400     400

  Fragments arranged by size: 

                1419    1400     400
 
BmtI G_CTAG'C

Cuts at:      0     137    3219 
   Size:        137    3082
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1171    1181    2002    2480    3219 
   Size:       1171      10     821     478     739

  Fragments arranged by size: 

                1171     821     739     478      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15    1405    3219 
   Size:         15    1390    1814

  Fragments arranged by size: 

                1814    1390      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2213    3219 
   Size:       2213    1006
 
BsaBI GATnn'nnATC

Cuts at:      0    1717    2300    3219 
   Size:       1717     583     919

  Fragments arranged by size: 

                1717     919     583
 
BsaWI w'CCGG_w

Cuts at:      0    2132    3219 
   Size:       2132    1087
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     517     547    1501    1531    2357    2387    3219 
   Size:        517      30     954      30     826      30     832

  Fragments arranged by size: 

                 954     832     826     517      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1381    2809    3219 
   Size:       1381    1428     410

  Fragments arranged by size: 

                1428    1381     410
 
BseYI C'CCAG_C

Cuts at:      0     129     301    1008    1497    2744    3219 
   Size:        129     172     707     489    1247     475

  Fragments arranged by size: 

                1247     707     489     475     172     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     624    1525    2751    3219 
   Size:        619       5     901    1226     468

  Fragments arranged by size: 

                1226     901     619     468       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     875    3016    3219 
   Size:        875    2141     203

  Fragments arranged by size: 

                2141     875     203
 
Bsp1286I G_dGCh'C

Cuts at:      0     720     875    1419    1834    2819    3016    3219 
   Size:        720     155     544     415     985     197     203

  Fragments arranged by size: 

                 985     720     544     415     203     197     155
 
BspHI T'CATG_A

Cuts at:      0     824    3219 
   Size:        824    2395
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     590    1629    1773    2396    3219 
   Size:        590    1039     144     623     823

  Fragments arranged by size: 

                1039     823     623     590     144
 
BsrBI CCG'CTC

Cuts at:      0     209    3219 
   Size:        209    3010
 
BsrDI GCAATG_nn'

Cuts at:      0     402    1830    3074    3219 
   Size:        402    1428    1244     145

  Fragments arranged by size: 

                1428    1244     402     145
 
BsrGI T'GTAC_A

Cuts at:      0    1458    3219 
   Size:       1458    1761
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1635    3219 
   Size:       1635    1584
 
BstYI r'GATC_y

Cuts at:      0     269     435     653     737     864     881    1160    1378 
   Size:        269     166     218      84     127      17     279     218

Cuts at:   1378    3000    3067    3105    3140    3219 
   Size:       1622      67      38      35      79

  Fragments arranged by size: 

                1622     279     269     218     218     166     127      84
                  79      67      38      35      17
 
Bsu36I CC'TnA_GG

Cuts at:      0    2047    3219 
   Size:       2047    1172
 
BtgI C'CryG_G

Cuts at:      0    1790    3131    3219 
   Size:       1790    1341      88

  Fragments arranged by size: 

                1790    1341      88
 
BtsI GCAGTG_nn'

Cuts at:      0     602     607    2084    3219 
   Size:        602       5    1477    1135

  Fragments arranged by size: 

                1477    1135     602       5
 
EaeI y'GGCC_r

Cuts at:      0    1814    3145    3219 
   Size:       1814    1331      74

  Fragments arranged by size: 

                1814    1331      74
 
EarI CTCTTCn'nnn_

Cuts at:      0     418     551     764    2957    3219 
   Size:        418     133     213    2193     262

  Fragments arranged by size: 

                2193     418     262     213     133
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1927    3219 
   Size:       1927    1292
 
EcoICRI GAG'CTC

Cuts at:      0     873    3219 
   Size:        873    2346
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127    1171    1181    1195    1369    2002    2480    2706 
   Size:        127    1044      10      14     174     633     478     226

Cuts at:   2706    3219 
   Size:        513

  Fragments arranged by size: 

                1044     633     513     478     226     174     127      14
                  10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     408     972    3219 
   Size:        408     564    2247

  Fragments arranged by size: 

                2247     564     408
 
EcoRI G'AATT_C

Cuts at:      0     178    3219 
   Size:        178    3041
 
EcoRV GAT'ATC

Cuts at:      0    2763    3219 
   Size:       2763     456
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     400    2637    3219 
   Size:        400    2237     582

  Fragments arranged by size: 

                2237     582     400
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1707    1739    2091    2123    2320    2352    2599    2631 
   Size:       1707      32     352      32     197      32     247      32

Cuts at:   2631    3074    3106    3219 
   Size:        443      32     113

  Fragments arranged by size: 

                1707     443     352     247     197     113      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     663    3219 
   Size:        663    2556
 
KpnI G_GTAC'C

Cuts at:      0    2588    3219 
   Size:       2588     631
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2051    2976    3198    3219 
   Size:       2051     925     222      21

  Fragments arranged by size: 

                2051     925     222      21
 
MscI TGG'CCA

Cuts at:      0    1816    3219 
   Size:       1816    1403
 
MslI CAynn'nnrTG

Cuts at:      0    1247    2656    3019    3021    3219 
   Size:       1247    1409     363       2     198

  Fragments arranged by size: 

                1409    1247     363     198       2
 
MspA1I CmG'CkG

Cuts at:      0     105    1501    2544    3219 
   Size:        105    1396    1043     675

  Fragments arranged by size: 

                1396    1043     675     105
 
NcoI C'CATG_G

Cuts at:      0    1790    3131    3219 
   Size:       1790    1341      88

  Fragments arranged by size: 

                1790    1341      88
 
NheI G'CTAG_C

Cuts at:      0     133    3219 
   Size:        133    3086
 
NsiI A_TGCA'T

Cuts at:      0     897    3219 
   Size:        897    2322
 
NspI r_CATG'y

Cuts at:      0    1246    1990    2286    3219 
   Size:       1246     744     296     933

  Fragments arranged by size: 

                1246     933     744     296
 
PacI TTA_AT'TAA

Cuts at:      0     455    3219 
   Size:        455    2764
 
PciI A'CATG_T

Cuts at:      0    2282    3219 
   Size:       2282     937
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1268    3219 
   Size:       1268    1951
 
PfoI T'CCnGG_A

Cuts at:      0    2996    3219 
   Size:       2996     223
 
PpuMI rG'GwC_Cy

Cuts at:      0     972    3219 
   Size:        972    2247
 
PstI C_TGCA'G

Cuts at:      0    1640    3219 
   Size:       1640    1579
 
PvuII CAG'CTG

Cuts at:      0     105    1501    2544    3219 
   Size:        105    1396    1043     675

  Fragments arranged by size: 

                1396    1043     675     105
 
SacI G_AGCT'C

Cuts at:      0     875    3219 
   Size:        875    2344
 
SexAI A'CCwGG_T

Cuts at:      0    2681    3219 
   Size:       2681     538
 
SfcI C'TryA_G

Cuts at:      0    1636    1872    2527    3219 
   Size:       1636     236     655     692

  Fragments arranged by size: 

                1636     692     655     236
 
SmlI C'TyrA_G

Cuts at:      0      30    1420    3219 
   Size:         30    1390    1799

  Fragments arranged by size: 

                1799    1390      30
 
SphI G_CATG'C

Cuts at:      0    1246    3219 
   Size:       1246    1973
 
StyI C'CwwG_G

Cuts at:      0     551     968    1790    2111    2819    3131    3219 
   Size:        551     417     822     321     708     312      88

  Fragments arranged by size: 

                 822     708     551     417     321     312      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1324    1906    2689    3219 
   Size:       1324     582     783     530

  Fragments arranged by size: 

                1324     783     582     530
 
TatI w'GTAC_w

Cuts at:      0     725    1113    1458    1565    1574    3219 
   Size:        725     388     345     107       9    1645

  Fragments arranged by size: 

                1645     725     388     345     107       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1386    1418    2357    2389    3219 
   Size:       1386      32     939      32     830

  Fragments arranged by size: 

                1386     939     830      32      32
 
XbaI T'CTAG_A

Cuts at:      0     867    3219 
   Size:        867    2352
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2412    3057    3219 
   Size:       2412     645     162

  Fragments arranged by size: 

                2412     645     162
 
XmnI GAAnn'nnTTC

Cuts at:      0     959    2668    3219 
   Size:        959    1709     551

  Fragments arranged by size: 

                1709     959     551
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI
    BamHI     BanI    BanII     BbsI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmtI     BpmI    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BstAPI    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I
     NcoI     NheI     NsiI     NspI     PacI     PciI    PflMI     PfoI
    PpuMI     PstI    PvuII     SacI    SexAI     SfcI     SmlI     SphI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI     BcgI     BglI    BmgBI     BmrI     BplI   Bpu10I
    BsaAI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NotI     NruI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI  Tth111I     XhoI     XmaI     ZraI
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