Restriction map for pF1KSDA0122
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AflIII      
AgeI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        AspI        AvrII       BbeI        
BbrPI       BciVI       BclI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BstBI       
Bsu36I      ClaI        DraI        EarI        Eco47III    
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KasI        Ksp632I     
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        SapI        ScaI        SexAI       SfoI        
SfuI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tru9I       Tth111I     XbaI        
XcmI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        1699  GACGTC               GACGT^C
Acc65I       1264  GGTACC               G^GTACC
AhdI         1462  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2696  GTGCAC               G^TGCAC
ApaLI        2696  GTGCAC               G^TGCAC
Asp700I       693  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1264  GGTACC               G^GTACC
AspEI        1462  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         911  TGCGCA               TGC^GCA
BamHI        1416  GGATCC               G^GATCC
BbsI         1350  GTCTTC               GAAGAC(2/6)
BbvCI         396  GCTGAGG              CCTCAGC(-5/-2)
BlpI          792  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      792  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1350  GTCTTC               GAAGAC(2/6)
BspMI        1429  GCAGGT               ACCTGC(4/8)
BsrDI        1215  CATTGC               GCAATG(2/0)
BssSI         750  CACGAG               CACGAG(-5/-1)
Bst1107I     1575  GTATAC               GTA^TAC
BstXI        1952  CCAAGGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1575  GTATAC               GTA^TAC
CelII         792  GCTGAGC              GC^T[ACGT]AGC
CpoI          322  CGGACCG              CG^G[AT]CCG
DrdI           14  GACGTGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1862  CGGCCG               C^GGCCG
Eam1105I     1462  GACTCTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1862  CGGCCG               C^GGCCG
Eco52I       1862  CGGCCG               C^GGCCG
FspI          911  TGCGCA               TGC^GCA
HaeII        2313  GGCGCT               [GA]GCGC^[CT]
HincII       1294  GTCGAC               GT[CT]^[GA]AC
HindII       1294  GTCGAC               GT[CT]^[GA]AC
KpnI         1264  GGTACC               GGTAC^C
KspI         1026  CCGCGG               CCGC^GG
NruI          135  TCGCGA               TCG^CGA
PaeR7I       2086  CTCGAG               C^TCGAG
PpuMI        1431  AGGTCCC              [GA]G^G[AT]CC[CT]
RsrII         322  CGGACCG              CG^G[AT]CCG
SacI         1609  GAGCTC               GAGCT^C
SacII        1026  CCGCGG               CCGC^GG
SalI         1294  GTCGAC               G^TCGAC
SbfI         1083  CCTGCAGG             CCTGCA^GG
SfiI         1224  GGCCCAGTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2368  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          219  CCCGGG               CCC^GGG
Sse8387I     1083  CCTGCAGG             CCTGCA^GG
Tsp509I      2141  AATT                 ^AATT
XhoI         2086  CTCGAG               C^TCGAG
XmaCI         219  CCCGGG               C^CCGGG
XmaI          219  CCCGGG               C^CCGGG
XmnI          693  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BcgI          688  CGAGAGAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2544  GCAGCCTACTCG        
BsaI           95  GAGACC               GGTCTC(1/5)
             1723  GAGACC              
BsmBI        1701  CGTCTC               CGTCTC(1/5)
              293  GAGACG              
BsrBI          60  CCGCTC               CCGCTC(-3/-3)
             2239  GAGCGG              
BstEII       2718  GGTCACC              G^GT[ACGT]ACC
             2763  GGTGACC             
BstPI        2718  GGTCACC              G^GT[ACGT]ACC
             2763  GGTGACC             
DraIII        454  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
              776  CACTGGGTG           
EcoNI         858  CCTGTCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2167  CCTCCGCGAGG         
EcoO65I      2718  GGTCACC              G^GT[ACGT]ACC
             2763  GGTGACC             
PflMI         775  CCACTGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2600  CCATGGGCTGG         
Van91I        775  CCACTGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2600  CCATGGGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
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