Restriction map for pF1KSDA0106
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmBI       Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MluI        MluNI       MroI        MscI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AcsI          178  GAATTT               [GA]^AATT[CT]
Alw44I        138  GTGCAC               G^TGCAC
ApaLI         138  GTGCAC               G^TGCAC
ApoI          178  GAATTT               [GA]^AATT[CT]
BamHI         351  GGATCC               G^GATCC
BanI          171  GGCACC               G^G[CT][GA]CC
BbsI          616  GTCTTC               GAAGAC(2/6)
BbvI          160  GCTGC                GCAGC(8/12)
BcnI            4  CCCGG                CC^[GC]GG
BpuAI         616  GTCTTC               GAAGAC(2/6)
BsaBI         313  GATAGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         118  CCTCGG               C^C[ACGT][ACGT]GG
BsiEI          60  CGGCCG               CG[GA][CT]^CG
BsmAI          89  GAGAC                GTCTC(1/5)
BsmI          101  GCATTC               GAATGC(1/-1)
BspDI         307  ATCGAT               AT^CGAT
BssSI         392  CTCGTG               CACGAG(-5/-1)
BstNI         242  CCTGG                CC^[AT]GG
BstYI         351  GGATCC               [GA]^GATC[CT]
ClaI          307  ATCGAT               AT^CGAT
DdeI          481  CTCAG                C^T[ACGT]AG
DpnII         352  GATC                 ^GATC
EaeI           60  CGGCCG               [CT]^GGCC[GA]
EagI           60  CGGCCG               C^GGCCG
EclXI          60  CGGCCG               C^GGCCG
Eco52I         60  CGGCCG               C^GGCCG
EcoRII        242  CCTGG                ^CC[AT]GG
EcoRV         253  GATATC               GAT^ATC
HapII           5  CCGG                 C^CGG
HpaII           5  CCGG                 C^CGG
HphI          620  TCACC                GGTGA(8/7)
MaeII          26  ACGT                 A^CGT
MamI          313  GATAGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          352  GATC                 ^GATC
MfeI          267  CAATTG               C^AATTG
MflI          351  GGATCC               [GA]^GATC[CT]
MseI          197  TTAA                 T^TAA
MslI          378  CATGCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        502  CAGCTG               C[AC]G^C[GT]G
MspI            5  CCGG                 C^CGG
MunI          267  CAATTG               C^AATTG
MvaI          242  CCTGG                CC^[AT]GG
MwoI          163  GCAAAGCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI            4  CCCGG                CC^[GC]GG
NdeII         352  GATC                 ^GATC
PvuII         502  CAGCTG               CAG^CTG
SacI          628  GAGCTC               GAGCT^C
SapI          274  GAAGAGC              GCTCTTC(1/4)
Sau3AI        352  GATC                 ^GATC
SfaNI          65  GCATC                GCATC(5/9)
TaqI          308  TCGA                 T^CGA
Tru9I         197  TTAA                 T^TAA
TseI          160  GCTGC                G^C[AT]GC
Tsp45I        385  GTGAC                ^GT[GC]AC
TthHB8I       308  TCGA                 T^CGA
XhoII         351  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI           63  CCGC                 CCGC(-3/-1)
              654  CCGC                
AlwI          352  GATCC                GGATC(4/5)
              351  GGATC               
AspHI         138  GTGCAC               G[AT]GC[AT]^C
              628  GAGCTC              
AvaI           19  CTCGGG               C^[CT]CG[GA]G
              119  CTCGGG              
BanII         277  GAGCCC               G[GA]GC[CT]^C
              628  GAGCTC              
BpmI          499  CTCCAG               CTGGAG(16/14)
              243  CTGGAG              
BsiHKAI       138  GTGCAC               G[AT]GC[AT]^C
              628  GAGCTC              
BsmFI          23  GGGAC                GGGAC(10/14)
              122  GGGAC               
BsoBI          19  CTCGGG               C^[CT]CG[GA]G
              119  CTCGGG              
BssKI           4  CCCGG                ^CC[ACGT]GG
              242  CCTGG               
BstXI         332  CCATCCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              532  CCAGTTGATTGG        
EarI          106  CTCTTC               CTCTTC(1/4)
              274  GAAGAG              
Fnu4HI         62  GCCGC                GC^[ACGT]GC
              160  GCTGC               
HaeIII         45  GGCC                 GG^CC
               61  GGCC                
ItaI           62  GCCGC                GC^[ACGT]GC
              160  GCTGC               
Ksp632I       106  CTCTTC               CTCTTC(1/4)
              274  GAAGAG              
MaeIII        291  GTTAC                ^GT[ACGT]AC
              385  GTGAC               
NspI          344  GCATGC               [GA]CATG^[CT]
              377  GCATGC              
PleI           91  GACTC                GAGTC(4/5)
              614  GAGTC               
ScrFI           4  CCCGG                CC^[ACGT]GG
              242  CCTGG               
SphI          344  GCATGC               GCATG^C
              377  GCATGC              
TfiI          318  GAATC                G^A[AT]TC
              477  GATTC               
Tsp509I       179  AATT                 ^AATT
              268  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        AvaII       BmyI        BsiYI       BslI        
Bsp1286I    BsrI        BstF5I      Cac8I       Cfr13I      
FokI        HinfI       MboII       MnlI        NlaIII      
NlaIV       Sau96I      TspRI       
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