Restriction map for pF1KSDA0106
 (Circular) (Six-base) MAPSORT of: pF1KSDA0106.seq  Check: 5930  from: 1  to: 3809



pF1KSDA0106  3809 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    847     847 
   Size:       3809
 
Acc65I G'GTAC_C

Cuts at:    825     825 
   Size:       3809
 
AclI AA'CG_TT

Cuts at:   3649    3649 
   Size:       3809
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    574     728    1634    2066    2813    3081     574 
   Size:        154     906     432     747     268    1302

  Fragments arranged by size: 

                1302     906     747     432     268     154
 
AflIII A'CryG_T

Cuts at:   1161    2265    3242    1161 
   Size:       1104     977    1728

  Fragments arranged by size: 

                1728    1104     977
 
AgeI A'CCGG_T

Cuts at:   2197    2197 
   Size:       3809
 
AlwNI CAG_nnn'CTG

Cuts at:   1114    2681    1114 
   Size:       1567    2242
 
ApaLI G'TGCA_C

Cuts at:    264    2579     264 
   Size:       2315    1494
 
ApoI r'AATT_y

Cuts at:    304     813    3162    3418     304 
   Size:        509    2349     256     695

  Fragments arranged by size: 

                2349     695     509     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3809
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3809
 
AvaI C'yCGr_G

Cuts at:    145     245     829     145 
   Size:        100     584    3125

  Fragments arranged by size: 

                3125     584     100
 
BamHI G'GATC_C

Cuts at:    477     834     477 
   Size:        357    3452
 
BanI G'GyrC_C

Cuts at:    297     825    1239    1487    1522     297 
   Size:        528     414     248      35    2584

  Fragments arranged by size: 

                2584     528     414     248      35
 
BanII G_rGCy'C

Cuts at:    407     758     823    1853     407 
   Size:        351      65    1030    2363

  Fragments arranged by size: 

                2363    1030     351      65
 
BbeI G_GCGC'C

Cuts at:   1243    1491    1243 
   Size:        248    3561
 
BbsI GAAGACnn'nnnn_

Cuts at:    735     735 
   Size:       3809
 
BciVI GTATCCnnnnn_n'

Cuts at:   1707    2468    3809    1707 
   Size:        761    1341    1707

  Fragments arranged by size: 

                1707    1341     761
 
BclI T'GATC_A

Cuts at:   1329    1329 
   Size:       3809
 
BglII A'GATC_T

Cuts at:   1324    3361    1324 
   Size:       2037    1772
 
BlpI GC'TnA_GC

Cuts at:    921     921 
   Size:       3809
 
Bme1580I G_kGCm'C

Cuts at:    268    1434    1527    2583     268 
   Size:       1166      93    1056    1494

  Fragments arranged by size: 

                1494    1166    1056      93
 
BmgBI CAC'GTC

Cuts at:    153     153 
   Size:       3809
 
BmrI ACTGGGnnnn_n'

Cuts at:    253     652    1021    1102    1437    3581     253 
   Size:        399     369      81     335    2144     481

  Fragments arranged by size: 

                2144     481     399     369     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2176    2176 
   Size:       3809
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    390     610     390 
   Size:        220    3589
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    985    1864    2356    2654     985 
   Size:        879     492     298    2140

  Fragments arranged by size: 

                2140     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3513    3513 
   Size:       3809
 
BsaAI yAC'GTr

Cuts at:    801    1162     801 
   Size:        361    3448
 
BsaBI GATnn'nnATC

Cuts at:      6     443    1323    3360       6 
   Size:        437     880    2037     455

  Fragments arranged by size: 

                2037     880     455     437
 
BsaHI Gr'CG_yC

Cuts at:   1240    1488    2190    3686    1240 
   Size:        248     702    1496    1363

  Fragments arranged by size: 

                1496    1363     702     248
 
BsaWI w'CCGG_w

Cuts at:    998    1519    2197    2471    2618    3029     998 
   Size:        521     678     274     147     411    1778

  Fragments arranged by size: 

                1778     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    573     791    1234    1992    2569    3065     573 
   Size:        218     443     758     577     496    1317

  Fragments arranged by size: 

                1317     758     577     496     443     218
 
BsiEI CG_ry'CG

Cuts at:    104     189    1351    1397    2605     104 
   Size:         85    1162      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1162      85      46
 
BsiHKAI G_wGCw'C

Cuts at:    268     758     823    1601    1791    2583     268 
   Size:        490      65     778     190     792    1494

  Fragments arranged by size: 

                1494     792     778     490     190      65
 
BsmI GAATG_Cn'

Cuts at:    227     227 
   Size:       3809
 
Bsp1286I G_dGCh'C

Cuts at:    268     407     758     823    1434    1527    1601    1791    1853 
   Size:        139     351      65     611      93      74     190      62

Cuts at:   1853    2583     268 
   Size:        730    1494

  Fragments arranged by size: 

                1494     730     611     351     190     139      93      74
                  65      62
 
BspEI T'CCGG_A

Cuts at:   3029    3029 
   Size:       3809
 
BspHI T'CATG_A

Cuts at:   2985    2985 
   Size:       3809
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    859    1073    1375    1756     859 
   Size:        214     302     381    2912

  Fragments arranged by size: 

                2912     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2101    2155    3639    3804      50 
   Size:       2051      54    1484     165      55

  Fragments arranged by size: 

                2051    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1721    1721 
   Size:       3809
 
BsrFI r'CCGG_y

Cuts at:   1807    1988    2197    1807 
   Size:        181     209    3419

  Fragments arranged by size: 

                3419     209     181
 
BssSI C'ACGA_G

Cuts at:    518    2080    2438     518 
   Size:       1562     358    1889

  Fragments arranged by size: 

                1889    1562     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1186    1186 
   Size:       3809
 
BstBI TT'CG_AA

Cuts at:    110    2170     110 
   Size:       2060    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    465     665     465 
   Size:        200    3609
 
BstYI r'GATC_y

Cuts at:      1     477     834    1301    1324    1659    1905    2917    3003 
   Size:        476     357     467      23     335     246    1012      86

Cuts at:   3003    3015    3361       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     476     467     449     357     346     335     246
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1759    1940    2103    1759 
   Size:        181     163    3465

  Fragments arranged by size: 

                3465     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1052    1052 
   Size:       3809
 
ClaI AT'CG_AT

Cuts at:    434     434 
   Size:       3809
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    258     293     642     677     258 
   Size:         35     349      35    3390

  Fragments arranged by size: 

                3390     349      35      35
 
DraI TTT'AAA

Cuts at:    808     808 
   Size:       3809
 
DrdI GACnn_nn'nnGTC

Cuts at:   1515    2373    1515 
   Size:        858    2951
 
EaeI y'GGCC_r

Cuts at:    186    1394    1568    1959    1986    3331     186 
   Size:       1208     174     391      27    1345     664

  Fragments arranged by size: 

                1345    1208     664     391     174      27
 
EagI C'GGCC_G

Cuts at:    186    1394     186 
   Size:       1208    2601
 
EarI CTCTTCn'nnn_

Cuts at:    238     395    1832    2042     238 
   Size:        157    1437     210    2005

  Fragments arranged by size: 

                2005    1437     210     157
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2017    2327    2473    3619    2017 
   Size:        310     146    1146    2207

  Fragments arranged by size: 

                2207    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    756     821     756 
   Size:         65    3744
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    390     574     610     728    1634    2066    2813    3081     390 
   Size:        184      36     118     906     432     747     268    1118

  Fragments arranged by size: 

                1118     906     747     432     268     184     118      36
            
 
EcoO109I rG'GnC_Cy

Cuts at:    948     948 
   Size:       3809
 
EcoRI G'AATT_C

Cuts at:    813     813 
   Size:       3809
 
EcoRV GAT'ATC

Cuts at:    381     381 
   Size:       3809
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1828    1828 
   Size:       3809
 
FspI TGC'GCA

Cuts at:   1590    1590 
   Size:       3809
 
HaeII r_GCGC'y

Cuts at:   1243    1491    2513    3492    1243 
   Size:        248    1022     979    1560

  Fragments arranged by size: 

                1560    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    745     777    1651    1681    1683    1713     745 
   Size:         32     874      30       2      30    2841

  Fragments arranged by size: 

                2841     874      32      30      30       2
 
HincII GTy'rAC

Cuts at:    848     848 
   Size:       3809
 
KasI G'GCGC_C

Cuts at:   1239    1487    1239 
   Size:        248    3561
 
KpnI G_GTAC'C

Cuts at:    829     829 
   Size:       3809
 
MfeI C'AATT_G

Cuts at:    393     393 
   Size:       3809
 
MluI A'CGCG_T

Cuts at:   3242    3242 
   Size:       3809
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    721    2480    2664     721 
   Size:       1759     184    1866

  Fragments arranged by size: 

                1866    1759     184
 
MscI TGG'CCA

Cuts at:   1570    1570 
   Size:       3809
 
MslI CAynn'nnrTG

Cuts at:    508    1798    1925     508 
   Size:       1290     127    2392

  Fragments arranged by size: 

                2392    1290     127
 
MspA1I CmG'CkG

Cuts at:    630     920    1234    1594    2607    2852     630 
   Size:        290     314     360    1013     245    1587

  Fragments arranged by size: 

                1587    1013     360     314     290     245
 
NaeI GCC'GGC

Cuts at:   1990    1990 
   Size:       3809
 
NarI GG'CG_CC

Cuts at:   1240    1488    1240 
   Size:        248    3561
 
NgoMIV G'CCGG_C

Cuts at:   1988    1988 
   Size:       3809
 
NspI r_CATG'y

Cuts at:    474     507     862    1362    2269     474 
   Size:         33     355     500     907    2014

  Fragments arranged by size: 

                2014     907     500     355      33
 
PciI A'CATG_T

Cuts at:   2265    2265 
   Size:       3809
 
PmeI GTTT'AAAC

Cuts at:    808     808 
   Size:       3809
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2948    2980    2948 
   Size:         32    3777
 
PstI C_TGCA'G

Cuts at:    856    1541     856 
   Size:        685    3124
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3809
 
PvuII CAG'CTG

Cuts at:    630    1234    1594     630 
   Size:        604     360    2845

  Fragments arranged by size: 

                2845     604     360
 
RsrII CG'GwC_CG

Cuts at:   2004    2004 
   Size:       3809
 
SacI G_AGCT'C

Cuts at:    758     823     758 
   Size:         65    3744
 
SalI G'TCGA_C

Cuts at:    846     846 
   Size:       3809
 
SapI GCTCTTCn'nnn_

Cuts at:    395    1832    2042     395 
   Size:       1437     210    2162

  Fragments arranged by size: 

                2162    1437     210
 
SbfI CC_TGCA'GG

Cuts at:    856     856 
   Size:       3809
 
ScaI AGT'ACT

Cuts at:   3326    3326 
   Size:       3809
 
SfcI C'TryA_G

Cuts at:     33     852    1537    2530    2721      33 
   Size:        819     685     993     191    1121

  Fragments arranged by size: 

                1121     993     819     685     191
 
SfoI GGC'GCC

Cuts at:   1241    1489    1241 
   Size:        248    3561
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3809
 
SmaI CCC'GGG

Cuts at:    831     831 
   Size:       3809
 
SmlI C'TyrA_G

Cuts at:    964    1879    2371    2633     964 
   Size:        915     492     262    2140

  Fragments arranged by size: 

                2140     915     492     262
 
SnaBI TAC'GTA

Cuts at:    801     801 
   Size:       3809
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3809
 
SphI G_CATG'C

Cuts at:    474     507     862    1362     474 
   Size:         33     355     500    2921

  Fragments arranged by size: 

                2921     500     355      33
 
StyI C'CwwG_G

Cuts at:    943    1297     943 
   Size:        354    3455
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1392    1525    1984    2193    1392 
   Size:        133     459     209    3008

  Fragments arranged by size: 

                3008     459     209     133
 
TatI w'GTAC_w

Cuts at:   3324    3324 
   Size:       3809
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3326    3358    3326 
   Size:         32    3777
 
Tth111I GACn'n_nGTC

Cuts at:   1606    1606 
   Size:       3809
 
XbaI T'CTAG_A

Cuts at:    840     840 
   Size:       3809
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1304    1304 
   Size:       3809
 
XmaI C'CCGG_G

Cuts at:    829     829 
   Size:       3809
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI    BsmBI    BsrGI
   BssHII   BstEII  BstZ17I   Bsu36I     BtgI   DraIII    EcoNI     FseI
    FspAI  HindIII     HpaI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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