Restriction map for pF1KSDA0106
 (Linear) (Six-base) MAPSORT of: KIAA0106.seq  Check: 6964  from: 1  to: 672



KIAA0106.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     602     672 
   Size:        448     154      70

  Fragments arranged by size: 

                 448     154      70
 
ApaLI G'TGCA_C

Cuts at:      0     138     672 
   Size:        138     534
 
ApoI r'AATT_y

Cuts at:      0     178     672 
   Size:        178     494
 
AvaI C'yCGr_G

Cuts at:      0      19     119     672 
   Size:         19     100     553

  Fragments arranged by size: 

                 553     100      19
 
BamHI G'GATC_C

Cuts at:      0     351     672 
   Size:        351     321
 
BanI G'GyrC_C

Cuts at:      0     171     672 
   Size:        171     501
 
BanII G_rGCy'C

Cuts at:      0     281     632     672 
   Size:        281     351      40

  Fragments arranged by size: 

                 351     281      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     609     672 
   Size:        609      63
 
Bme1580I G_kGCm'C

Cuts at:      0     142     672 
   Size:        142     530
 
BmgBI CAC'GTC

Cuts at:      0      27     672 
   Size:         27     645
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     127     526     672 
   Size:        127     399     146

  Fragments arranged by size: 

                 399     146     127
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     484     672 
   Size:        264     220     188

  Fragments arranged by size: 

                 264     220     188
 
BsaBI GATnn'nnATC

Cuts at:      0     317     672 
   Size:        317     355
 
BseYI C'CCAG_C

Cuts at:      0     447     665     672 
   Size:        447     218       7

  Fragments arranged by size: 

                 447     218       7
 
BsiEI CG_ry'CG

Cuts at:      0      63     672 
   Size:         63     609
 
BsiHKAI G_wGCw'C

Cuts at:      0     142     632     672 
   Size:        142     490      40

  Fragments arranged by size: 

                 490     142      40
 
BsmI GAATG_Cn'

Cuts at:      0     101     672 
   Size:        101     571
 
Bsp1286I G_dGCh'C

Cuts at:      0     142     281     632     672 
   Size:        142     139     351      40

  Fragments arranged by size: 

                 351     142     139      40
 
BssSI C'ACGA_G

Cuts at:      0     392     672 
   Size:        392     280
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     339     539     672 
   Size:        339     200     133

  Fragments arranged by size: 

                 339     200     133
 
BstYI r'GATC_y

Cuts at:      0     351     672 
   Size:        351     321
 
ClaI AT'CG_AT

Cuts at:      0     308     672 
   Size:        308     364
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     132     167     516     551     672 
   Size:        132      35     349      35     121

  Fragments arranged by size: 

                 349     132     121      35      35
 
EaeI y'GGCC_r

Cuts at:      0      60     672 
   Size:         60     612
 
EagI C'GGCC_G

Cuts at:      0      60     672 
   Size:         60     612
 
EarI CTCTTCn'nnn_

Cuts at:      0     112     269     672 
   Size:        112     157     403

  Fragments arranged by size: 

                 403     157     112
 
EcoICRI GAG'CTC

Cuts at:      0     630     672 
   Size:        630      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     448     484     602     672 
   Size:        264     184      36     118      70

  Fragments arranged by size: 

                 264     184     118      70      36
 
EcoRV GAT'ATC

Cuts at:      0     255     672 
   Size:        255     417
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     619     651     672 
   Size:        619      32      21

  Fragments arranged by size: 

                 619      32      21
 
MfeI C'AATT_G

Cuts at:      0     267     672 
   Size:        267     405
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     672 
   Size:        595      77
 
MslI CAynn'nnrTG

Cuts at:      0     382     672 
   Size:        382     290
 
MspA1I CmG'CkG

Cuts at:      0     504     672 
   Size:        504     168
 
NspI r_CATG'y

Cuts at:      0     348     381     672 
   Size:        348      33     291

  Fragments arranged by size: 

                 348     291      33
 
PvuII CAG'CTG

Cuts at:      0     504     672 
   Size:        504     168
 
SacI G_AGCT'C

Cuts at:      0     632     672 
   Size:        632      40
 
SapI GCTCTTCn'nnn_

Cuts at:      0     269     672 
   Size:        269     403
 
SphI G_CATG'C

Cuts at:      0     348     381     672 
   Size:        348      33     291

  Fragments arranged by size: 

                 348     291      33
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI
 Bme1580I    BmgBI     BmrI     BpmI    BsaBI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BssSI    BstXI    BstYI     ClaI    CspCI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoRV    Hin4I     MfeI     MmeI
     MslI   MspA1I     NspI    PvuII     SacI     SapI     SphI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EciI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com