Restriction map for pF1KSDA0096
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        AseI        AsnI        
AspEI       AvrII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BlnI        
BlpI        Bpu1102I    BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       FseI        HaeII       
HpaI        KasI        KspI        MfeI        MluI        
MroI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1861  GGTACC               G^GTACC
AccII        1152  CGCG                 CG^CG
AcyI          931  GACGCC               G[GA]^CG[CT]C
AocI         2242  CCTAAGG              CC^T[ACGT]AGG
ApaI         1714  GGGCCC               GGGCC^C
Asp700I       767  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1861  GGTACC               G^GTACC
AspI         1186  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2260  TGCGCA               TGC^GCA
BamHI        1004  GGATCC               G^GATCC
BclI          677  TGATCA               T^GATCA
BcnI         1599  CCGGG                CC^[GC]GG
BsaAI        2049  TACGTG               [CT]AC^GT[GA]
BsaBI        1504  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         931  GACGCC               G[GA]^CG[CT]C
BsaI         1624  GAGACC               GGTCTC(1/5)
BsaWI        1580  ACCGGA               [AT]^CCGG[AT]
BsgI          208  GTGCAG               GTGCAG(16/14)
BsmBI         929  GAGACG               CGTCTC(1/5)
BsmI         2079  GAATGC               GAATGC(1/-1)
BspHI        1946  TCATGA               T^CATGA
BsrBI         542  CCGCTC               CCGCTC(-3/-3)
BsrDI        1760  GCAATG               GCAATG(2/0)
BstUI        1152  CGCG                 CG^CG
Bsu36I       2242  CCTAAGG              CC^T[ACGT]AGG
DsaI           83  CCGTGG               C^C[GA][CT]GG
Eco81I       2242  CCTAAGG              CC^T[ACGT]AGG
EcoT22I       195  ATGCAT               ATGCA^T
FbaI          677  TGATCA               T^GATCA
FspI         2260  TGCGCA               TGC^GCA
HgaI          931  GACGC                GACGC(5/10)
Hin1I         931  GACGCC               G[GA]^CG[CT]C
HincII        465  GTTGAC               GT[CT]^[GA]AC
HindII        465  GTTGAC               GT[CT]^[GA]AC
HindIII      1290  AAGCTT               A^AGCTT
KpnI         1861  GGTACC               GGTAC^C
MamI         1504  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1152  CGCG                 CG^CG
NciI         1599  CCGGG                CC^[GC]GG
NsiI          195  ATGCAT               ATGCA^T
NspI          102  GCATGT               [GA]CATG^[CT]
PflFI        1186  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2002  CCACAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1946  TCATGA               T^CATGA
SacI         1924  GAGCTC               GAGCT^C
SmlI         1117  CTTGAG               C^T[CT][GA]AG
SpeI          204  ACTAGT               A^CTAGT
StuI         1215  AGGCCT               AGG^CCT
Tsp45I       1223  GTGAC                ^GT[GC]AC
Tth111I      1186  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2002  CCACAGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          273  TCTAGA               T^CTAGA
XmnI          767  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AcsI          490  AAATTT               [GA]^AATT[CT]
              551  AAATTC              
ApoI          490  AAATTT               [GA]^AATT[CT]
              551  AAATTC              
BbsI          337  GAAGAC               GAAGAC(2/6)
              436  GTCTTC              
BglII        1042  AGATCT               A^GATCT
             1463  AGATCT              
BpuAI         337  GAAGAC               GAAGAC(2/6)
              436  GTCTTC              
BsrFI        1649  GCCGGC               [GA]^CCGG[CT]
             1879  GCCGGC              
Cfr10I       1649  GCCGGC               [GA]^CCGG[CT]
             1879  GCCGGC              
EcoT14I        62  CCTTGG               C^C[AT][AT]GG
              799  CCTTGG              
MaeII        2050  ACGT                 A^CGT
             2186  ACGT                
MaeIII       1223  GTGAC                ^GT[ACGT]AC
             1417  GTAAC               
NaeI         1649  GCCGGC               GCC^GGC
             1879  GCCGGC              
NgoMIV       1649  GCCGGC               G^CCGGC
             1879  GCCGGC              
PleI         1225  GACTC                GAGTC(4/5)
              597  GAGTC               
PpuMI        1112  AGGACCT              [GA]G^G[AT]CC[CT]
             2090  AGGTCCC             
StyI           62  CCTTGG               C^C[AT][AT]GG
              799  CCTTGG              
XcmI         1766  CCAGTGGAGGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2141  CCACCACTGAATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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