Restriction map for pF1KSDA0093
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BalI        BamHI       BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlpI        Bpu1102I    
BsaBI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MluI        
MluNI       MroI        MscI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          12  CGCG                 CG^CG
AvaI         2492  CTCGGG               C^[CT]CG[GA]G
AvrII         306  CCTAGG               C^CTAGG
BanI         2496  GGTGCC               G^G[CT][GA]CC
BlnI          306  CCTAGG               C^CTAGG
BsaAI         127  TACGTG               [CT]AC^GT[GA]
BsaWI        1670  ACCGGA               [AT]^CCGG[AT]
BsmI          903  GAATGC               GAATGC(1/-1)
BsoBI        2492  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1140  TGTACA               T^GTACA
BspHI        2013  TCATGA               T^CATGA
BspMI        1230  GCAGGT               ACCTGC(4/8)
BsrFI         254  ACCGGC               [GA]^CCGG[CT]
BsrGI        1140  TGTACA               T^GTACA
BstAPI        718  GCACAACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1233  GGTGACC              G^GT[ACGT]ACC
BstPI        1233  GGTGACC              G^GT[ACGT]ACC
BstUI          12  CGCG                 CG^CG
BstXI        1051  CCACCAGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           11  GCGC                 GCG^C
Cfr10I        254  ACCGGC               [GA]^CCGG[CT]
DraI         2254  TTTAAA               TTT^AAA
DraIII        719  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1550  CCTACTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1233  GGTGACC              G^GT[ACGT]ACC
HhaI           11  GCGC                 GCG^C
HinP1I         11  GCGC                 G^CGC
HincII        769  GTTGAC               GT[CT]^[GA]AC
HindII        769  GTTGAC               GT[CT]^[GA]AC
MfeI         2666  CAATTG               C^AATTG
MspA1I       1165  CAGCTG               C[AC]G^C[GT]G
MunI         2666  CAATTG               C^AATTG
MvnI           12  CGCG                 CG^CG
NspI         1290  GCATGC               [GA]CATG^[CT]
PpuMI         616  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2537  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1187  CTGCAG               CTGCA^G
PvuII        1165  CAGCTG               CAG^CTG
RcaI         2013  TCATGA               T^CATGA
SexAI        1053  ACCAGGT              A^CC[AT]GGT
SmlI         1173  CTCAAG               C^T[CT][GA]AG
SphI         1290  GCATGC               GCATG^C
SspBI        1140  TGTACA               T^GTACA
XbaI          640  TCTAGA               T^CTAGA
XcmI         2679  CCAGGGCTTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI         1141  GTAC                 GT^AC
             2567  GTAC                
AlwNI         533  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              686  CAGATGCTG           
AocI          670  CCTCAGG              CC^T[ACGT]AGG
             2091  CCTCAGG             
AspHI         954  GAGCTC               G[AT]GC[AT]^C
             2588  GAGCTC              
BbsI         1660  GAAGAC               GAAGAC(2/6)
              783  GTCTTC              
BbvCI         244  CCTCAGC              CCTCAGC(-5/-2)
              493  GCTGAGG             
BpuAI        1660  GAAGAC               GAAGAC(2/6)
              783  GTCTTC              
BsaI          363  GAGACC               GGTCTC(1/5)
             1156  GAGACC              
BseRI         572  CTCCTC               GAGGAG(10/8)
             1757  GAGGAG              
BsiHKAI       954  GAGCTC               G[AT]GC[AT]^C
             2588  GAGCTC              
Bsu36I        670  CCTCAGG              CC^T[ACGT]AGG
             2091  CCTCAGG             
Eco81I        670  CCTCAGG              CC^T[ACGT]AGG
             2091  CCTCAGG             
EcoRV         604  GATATC               GAT^ATC
              747  GATATC              
EcoT14I       306  CCTAGG               C^C[AT][AT]GG
              609  CCTTGG              
MslI         1450  CACACAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2131  CATCAACATG          
NlaIV          29  GGCTCC               GG[ACGT]^[ACGT]CC
             2496  GGTGCC              
RsaI         1141  GTAC                 GT^AC
             2567  GTAC                
SacI          954  GAGCTC               GAGCT^C
             2588  GAGCTC              
StyI          306  CCTAGG               C^C[AT][AT]GG
              609  CCTTGG              
TaqI          952  TCGA                 T^CGA
             1713  TCGA                
TthHB8I       952  TCGA                 T^CGA
             1713  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BanII       BbvI        BfaI        BmyI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspI        MvaI        MwoI        NdeII       
NlaIII      PflMI       PleI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspI        StuI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XhoII       
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