Restriction map for pF1KSDA0091
 (Circular) (Six-base) MAPSORT of: pF1KSDA0091.seq  Check: 3845  from: 1  to: 6293



pF1KSDA0091  6293 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    877    1378     877 
   Size:        501    5792
 
AccI GT'mk_AC

Cuts at:    371    3331     371 
   Size:       2960    3333
 
Acc65I G'GTAC_C

Cuts at:   3309    3309 
   Size:       6293
 
AclI AA'CG_TT

Cuts at:   6133    6133 
   Size:       6293
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    376    2258    2277    4118    4550    5297    5565     376 
   Size:       1882      19    1841     432     747     268    1104

  Fragments arranged by size: 

                1882    1841    1104     747     432     268      19
 
AflIII A'CryG_T

Cuts at:   1031    1496    1613    2173    2477    3645    4749    5726    1031 
   Size:        465     117     560     304    1168    1104     977    1598

  Fragments arranged by size: 

                1598    1168    1104     977     560     465     304     117
            
 
AgeI A'CCGG_T

Cuts at:   4681    4681 
   Size:       6293
 
AhdI GACnn_n'nnGTC

Cuts at:   1604    1604 
   Size:       6293
 
AleI CACnn'nnGTG

Cuts at:    880     880 
   Size:       6293
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    694     694 
   Size:       6293
 
AlwNI CAG_nnn'CTG

Cuts at:    714    1393    1461    2127    2300    3598    5165     714 
   Size:        679      68     666     173    1298    1567    1842

  Fragments arranged by size: 

                1842    1567    1298     679     666     173      68
 
ApaI G_GGCC'C

Cuts at:   1353    2166    1353 
   Size:        813    5480
 
ApaLI G'TGCA_C

Cuts at:   5063    5063 
   Size:       6293
 
ApoI r'AATT_y

Cuts at:    262     333    3297    5646    5902     262 
   Size:         71    2964    2349     256     653

  Fragments arranged by size: 

                2964    2349     653     256      71
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6293
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6293
 
AvaI C'yCGr_G

Cuts at:   1486    2158    3313    1486 
   Size:        672    1155    4466

  Fragments arranged by size: 

                4466    1155     672
 
BamHI G'GATC_C

Cuts at:   3318    3318 
   Size:       6293
 
BanI G'GyrC_C

Cuts at:   1343    2949    3067    3309    3723    3971    4006    1343 
   Size:       1606     118     242     414     248      35    3630

  Fragments arranged by size: 

                3630    1606     414     248     242     118      35
 
BanII G_rGCy'C

Cuts at:    637    1353    1582    2166    3307    4337     637 
   Size:        716     229     584    1141    1030    2593

  Fragments arranged by size: 

                2593    1141    1030     716     584     229
 
BbeI G_GCGC'C

Cuts at:   2953    3071    3727    3975    2953 
   Size:        118     656     248    5271

  Fragments arranged by size: 

                5271     656     248     118
 
BbvCI CC'TCA_GC

Cuts at:   1042    2630    1042 
   Size:       1588    4705
 
BciVI GTATCCnnnnn_n'

Cuts at:    304    2364    4191    4952    6293     304 
   Size:       2060    1827     761    1341     304

  Fragments arranged by size: 

                2060    1827    1341     761     304
 
BclI T'GATC_A

Cuts at:   1176    1877    2088    3813    1176 
   Size:        701     211    1725    3656

  Fragments arranged by size: 

                3656    1725     701     211
 
BglI GCCn_nnn'nGGC

Cuts at:   1725    1725 
   Size:       6293
 
BglII A'GATC_T

Cuts at:   1593    3808    5845    1593 
   Size:       2215    2037    2041

  Fragments arranged by size: 

                2215    2041    2037
 
BlpI GC'TnA_GC

Cuts at:    787    3405     787 
   Size:       2618    3675
 
Bme1580I G_kGCm'C

Cuts at:    354    1353    2166    3918    4011    5067     354 
   Size:        999     813    1752      93    1056    1580

  Fragments arranged by size: 

                1752    1580    1056     999     813      93
 
BmgBI CAC'GTC

Cuts at:    604    1032    2265     604 
   Size:        428    1233    4632

  Fragments arranged by size: 

                4632    1233     428
 
BmrI ACTGGGnnnn_n'

Cuts at:    791    2786    2948    3505    3586    3921    6065     791 
   Size:       1995     162     557      81     335    2144    1019

  Fragments arranged by size: 

                2144    1995    1019     557     335     162      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    640    4660     640 
   Size:       4020    2273
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    911    1361    2710    2798    2881    3077    3104     911 
   Size:        450    1349      88      83     196      27    4100

  Fragments arranged by size: 

                4100    1349     450     196      88      83      27
 
Bpu10I CC'TnA_GC

Cuts at:   1042    1862    2630    1042 
   Size:        820     768    4705

  Fragments arranged by size: 

                4705     820     768
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    517    3020    3469    4348    4840    5138     517 
   Size:       2503     449     879     492     298    1672

  Fragments arranged by size: 

                2503    1672     879     492     449     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   2870    5997    2870 
   Size:       3127    3166
 
BsaAI yAC'GTr

Cuts at:   2174    3285    3646    2174 
   Size:       1111     361    4821

  Fragments arranged by size: 

                4821    1111     361
 
BsaBI GATnn'nnATC

Cuts at:      6    3807    5844       6 
   Size:       3801    2037     455

  Fragments arranged by size: 

                3801    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    998    2525    2950    3068    3724    3972    4674    6170     998 
   Size:       1527     425     118     656     248     702    1496    1121

  Fragments arranged by size: 

                1527    1496    1121     702     656     425     248     118
            
 
BsaWI w'CCGG_w

Cuts at:   2255    2374    3482    4003    4681    4955    5102    5513    2255 
   Size:        119    1108     521     678     274     147     411    3035

  Fragments arranged by size: 

                3035    1108     678     521     411     274     147     119
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   3143    3173    3143 
   Size:         30    6263
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1770    2236    2239    2393    2713    1770 
   Size:        466       3     154     320    5350

  Fragments arranged by size: 

                5350     466     320     154       3
 
BseYI C'CCAG_C

Cuts at:   1582    1631    1893    2391    3718    4476    5053    5549    1582 
   Size:         49     262     498    1327     758     577     496    2326

  Fragments arranged by size: 

                2326    1327     758     577     498     496     262      49
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1940    2517    1940 
   Size:        577    5716
 
BsiEI CG_ry'CG

Cuts at:    104    1281    3025    3835    3881    5089     104 
   Size:       1177    1744     810      46    1208    1308

  Fragments arranged by size: 

                1744    1308    1208    1177     810      46
 
BsiHKAI G_wGCw'C

Cuts at:    731    1414    3307    4085    4275    5067     731 
   Size:        683    1893     778     190     792    1957

  Fragments arranged by size: 

                1957    1893     792     778     683     190
 
BsmI GAATG_Cn'

Cuts at:    663    1658     663 
   Size:        995    5298
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2938    2938 
   Size:       6293
 
Bsp1286I G_dGCh'C

Cuts at:    354     637     731    1353    1414    1582    2166    3307    3918 
   Size:        283      94     622      61     168     584    1141     611

Cuts at:   3918    4011    4085    4275    4337    5067     354 
   Size:         93      74     190      62     730    1580

  Fragments arranged by size: 

                1580    1141     730     622     611     584     283     190
                 168      94      93      74      62      61
 
BspEI T'CCGG_A

Cuts at:   2255    5513    2255 
   Size:       3258    3035
 
BspHI T'CATG_A

Cuts at:   1874    5469    1874 
   Size:       3595    2698
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    877    1378    3243    3343    3557    3859    4240     877 
   Size:        501    1865     100     214     302     381    2930

  Fragments arranged by size: 

                2930    1865     501     381     302     214     100
 
BsrBI CCG'CTC

Cuts at:     50    3065    4585    4639    6123    6288      50 
   Size:       3015    1520      54    1484     165      55

  Fragments arranged by size: 

                3015    1520    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    573    1143    1856    4205     573 
   Size:        570     713    2349    2661

  Fragments arranged by size: 

                2661    2349     713     570
 
BsrFI r'CCGG_y

Cuts at:   4291    4472    4681    4291 
   Size:        181     209    5903

  Fragments arranged by size: 

                5903     209     181
 
BssSI C'ACGA_G

Cuts at:    157    4564    4922     157 
   Size:       4407     358    1528

  Fragments arranged by size: 

                4407    1528     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3246    3670    3246 
   Size:        424    5869
 
BstBI TT'CG_AA

Cuts at:    110    4654     110 
   Size:       4544    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2673    2859    2994    2673 
   Size:        186     135    5972

  Fragments arranged by size: 

                5972     186     135
 
BstYI r'GATC_y

Cuts at:      1    1544    1593    3318    3785    3808    4143    4389    5401 
   Size:       1543      49    1725     467      23     335     246    1012

Cuts at:   5401    5487    5499    5845       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1725    1543    1012     467     449     346     335     246
                  86      49      23      12
 
BtgI C'CryG_G

Cuts at:    870    1512    3155     870 
   Size:        642    1643    4008

  Fragments arranged by size: 

                4008    1643     642
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1209    2450    2487    4243    4424    4587    1209 
   Size:       1241      37    1756     181     163    2915

  Fragments arranged by size: 

                2915    1756    1241     181     163      37
 
BtsI GCAGTG_nn'

Cuts at:    369     712    2362    3536     369 
   Size:        343    1650    1174    3126

  Fragments arranged by size: 

                3126    1650    1174     343
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    260     295     260 
   Size:         35    6258
 
DraI TTT'AAA

Cuts at:   1019    2570    2939    3292    1019 
   Size:       1551     369     353    4020

  Fragments arranged by size: 

                4020    1551     369     353
 
DrdI GACnn_nn'nnGTC

Cuts at:   3999    4857    3999 
   Size:        858    5435
 
EaeI y'GGCC_r

Cuts at:   1811    2465    3099    3878    4052    4443    4470    5815    1811 
   Size:        654     634     779     174     391      27    1345    2289

  Fragments arranged by size: 

                2289    1345     779     654     634     391     174      27
            
 
EagI C'GGCC_G

Cuts at:   3878    3878 
   Size:       6293
 
EarI CTCTTCn'nnn_

Cuts at:    352     609    2234    4316    4526     352 
   Size:        257    1625    2082     210    2119

  Fragments arranged by size: 

                2119    2082    1625     257     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4501    4811    4957    6103    4501 
   Size:        310     146    1146    4691

  Fragments arranged by size: 

                4691    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3305    3305 
   Size:       6293
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    376     911    1361    2258    2277    2710    2798    2881    3077 
   Size:        535     450     897      19     433      88      83     196

Cuts at:   3077    3104    4118    4550    5297    5565     376 
   Size:         27    1014     432     747     268    1104

  Fragments arranged by size: 

                1104    1014     897     747     535     450     433     432
                 268     196      88      83      27      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2998    2998 
   Size:       6293
 
EcoO109I rG'GnC_Cy

Cuts at:    222    1350    1458    2154    2162    2163    3044    3432     222 
   Size:       1128     108     696       8       1     881     388    3083

  Fragments arranged by size: 

                3083    1128     881     696     388     108       8       1
            
 
EcoRI G'AATT_C

Cuts at:    262    3297     262 
   Size:       3035    3258
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2718    4312    2718 
   Size:       1594    4699
 
FspI TGC'GCA

Cuts at:   4074    4074 
   Size:       6293
 
HaeII r_GCGC'y

Cuts at:   2787    2953    3071    3237    3727    3975    4997    5976    2787 
   Size:        166     118     166     490     248    1022     979    3104

  Fragments arranged by size: 

                3104    1022     979     490     248     166     166     118
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1590    1622    1891    1923    2372    2395    2404    2427    4135 
   Size:         32     269      32     449      23       9      23    1708

Cuts at:   4135    4165    4167    4197    1590 
   Size:         30       2      30    3686

  Fragments arranged by size: 

                3686    1708     449     269      32      32      30      30
                  23      23       9       2
 
HincII GTy'rAC

Cuts at:    138    1083    1494    3332     138 
   Size:        945     411    1838    3099

  Fragments arranged by size: 

                3099    1838     945     411
 
HindIII A'AGCT_T

Cuts at:    130     130 
   Size:       6293
 
KasI G'GCGC_C

Cuts at:   2949    3067    3723    3971    2949 
   Size:        118     656     248    5271

  Fragments arranged by size: 

                5271     656     248     118
 
KpnI G_GTAC'C

Cuts at:   3313    3313 
   Size:       6293
 
MfeI C'AATT_G

Cuts at:    375    2667     375 
   Size:       2292    4001
 
MluI A'CGCG_T

Cuts at:   5726    5726 
   Size:       6293
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4964    5148    4964 
   Size:        184    6109
 
MscI TGG'CCA

Cuts at:   1813    2467    4054    1813 
   Size:        654    1587    4052

  Fragments arranged by size: 

                4052    1587     654
 
MslI CAynn'nnrTG

Cuts at:    880    1825    1954    4282    4409     880 
   Size:        945     129    2328     127    2764

  Fragments arranged by size: 

                2764    2328     945     129     127
 
MspA1I CmG'CkG

Cuts at:   2629    3404    3718    4078    5091    5336    2629 
   Size:        775     314     360    1013     245    3586

  Fragments arranged by size: 

                3586    1013     775     360     314     245
 
NaeI GCC'GGC

Cuts at:   4474    4474 
   Size:       6293
 
NarI GG'CG_CC

Cuts at:   2950    3068    3724    3972    2950 
   Size:        118     656     248    5271

  Fragments arranged by size: 

                5271     656     248     118
 
NcoI C'CATG_G

Cuts at:    870    3155     870 
   Size:       2285    4008
 
NgoMIV G'CCGG_C

Cuts at:   4472    4472 
   Size:       6293
 
NruI TCG'CGA

Cuts at:   2506    2506 
   Size:       6293
 
NspI r_CATG'y

Cuts at:    652    1500    1617    2481    3346    3846    4753     652 
   Size:        848     117     864     865     500     907    2192

  Fragments arranged by size: 

                2192     907     865     864     848     500     117
 
PasI CC'CwG_GG

Cuts at:    630    2042    3224     630 
   Size:       1412    1182    3699

  Fragments arranged by size: 

                3699    1412    1182
 
PciI A'CATG_T

Cuts at:   1496    1613    2477    4749    1496 
   Size:        117     864    2272    3040

  Fragments arranged by size: 

                3040    2272     864     117
 
PflMI CCAn_nnn'nTGG

Cuts at:    232    2521    2559    3117     232 
   Size:       2289      38     558    3408

  Fragments arranged by size: 

                3408    2289     558      38
 
PfoI T'CCnGG_A

Cuts at:   3081    3081 
   Size:       6293
 
PmeI GTTT'AAAC

Cuts at:   3292    3292 
   Size:       6293
 
PmlI CAC'GTG

Cuts at:   2174    2174 
   Size:       6293
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5432    5464    5432 
   Size:         32    6261
 
PpuMI rG'GwC_Cy

Cuts at:   2154    2154 
   Size:       6293
 
PspOMI G'GGCC_C

Cuts at:   1349    2162    1349 
   Size:        813    5480
 
PstI C_TGCA'G

Cuts at:    719    3340    4025     719 
   Size:       2621     685    2987

  Fragments arranged by size: 

                2987    2621     685
 
PvuI CG_AT'CG

Cuts at:    104    3025     104 
   Size:       2921    3372
 
PvuII CAG'CTG

Cuts at:   2629    3718    4078    2629 
   Size:       1089     360    4844

  Fragments arranged by size: 

                4844    1089     360
 
RsrII CG'GwC_CG

Cuts at:   4488    4488 
   Size:       6293
 
SacI G_AGCT'C

Cuts at:   3307    3307 
   Size:       6293
 
SalI G'TCGA_C

Cuts at:   3330    3330 
   Size:       6293
 
SapI GCTCTTCn'nnn_

Cuts at:    352     609    4316    4526     352 
   Size:        257    3707     210    2119

  Fragments arranged by size: 

                3707    2119     257     210
 
SbfI CC_TGCA'GG

Cuts at:   3340    3340 
   Size:       6293
 
ScaI AGT'ACT

Cuts at:   2230    5810    2230 
   Size:       3580    2713
 
SfcI C'TryA_G

Cuts at:     33     715    3336    4021    5014    5205      33 
   Size:        682    2621     685     993     191    1121

  Fragments arranged by size: 

                2621    1121     993     685     682     191
 
SfoI GGC'GCC

Cuts at:   2951    3069    3725    3973    2951 
   Size:        118     656     248    5271

  Fragments arranged by size: 

                5271     656     248     118
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6293
 
SmaI CCC'GGG

Cuts at:   1488    3315    1488 
   Size:       1827    4466
 
SmlI C'TyrA_G

Cuts at:    532    3035    3448    4363    4855    5117     532 
   Size:       2503     413     915     492     262    1708

  Fragments arranged by size: 

                2503    1708     915     492     413     262
 
SnaBI TAC'GTA

Cuts at:   3285    3285 
   Size:       6293
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6293
 
SphI G_CATG'C

Cuts at:    652    3346    3846     652 
   Size:       2694     500    3099

  Fragments arranged by size: 

                3099    2694     500
 
StyI C'CwwG_G

Cuts at:    870     915    1795    2553    2852    3155    3427    3781     870 
   Size:         45     880     758     299     303     272     354    3382

  Fragments arranged by size: 

                3382     880     758     354     303     299     272      45
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1773    3876    4009    4468    4677    1773 
   Size:       2103     133     459     209    3389

  Fragments arranged by size: 

                3389    2103     459     209     133
 
TatI w'GTAC_w

Cuts at:   2228    2649    2729    5808    2228 
   Size:        421      80    3079    2713

  Fragments arranged by size: 

                3079    2713     421      80
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3143    3175    5810    5842    3143 
   Size:         32    2635      32    3594

  Fragments arranged by size: 

                3594    2635      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1297    1905    4090    1297 
   Size:        608    2185    3500

  Fragments arranged by size: 

                3500    2185     608
 
XbaI T'CTAG_A

Cuts at:   3324    3324 
   Size:       6293
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    627    2915    3162    3788     627 
   Size:       2288     247     626    3132

  Fragments arranged by size: 

                3132    2288     626     247
 
XmaI C'CCGG_G

Cuts at:   1486    3313    1486 
   Size:       1827    4466
 
XmnI GAAnn'nnTTC

Cuts at:    663     663 
   Size:       6293
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NruI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AloI     AscI    AvrII     BaeI     BbsI
     BcgI    BfrBI     BmtI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI   DraIII    EcoRV     FseI    FspAI     HpaI     NdeI
     NheI     NotI     NsiI     PacI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI     SrfI     SspI     StuI
     SwaI     XhoI     ZraI
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