Restriction map for pF1KSDA0088
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AgeI        
AhdI        ApoI        AscI        AseI        AsnI        
AspEI       AspI        BbeI        BbrPI       BcgI        
BfrI        BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BsrDI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        HindIII     KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII         796  GACGTC               GACGT^C
Acc65I       1697  GGTACC               G^GTACC
AccI         1108  GTAGAC               GT^[AC][GT]AC
AccII         634  CGCG                 CG^CG
AcyI          796  GACGTC               G[GA]^CG[CT]C
Alw44I       1444  GTGCAC               G^TGCAC
ApaLI        1444  GTGCAC               G^TGCAC
Asp700I      1870  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1697  GGTACC               G^GTACC
AviII        2492  TGCGCA               TGC^GCA
AvrII         259  CCTAGG               C^CTAGG
BamHI         482  GGATCC               G^GATCC
BanI         1697  GGTACC               G^G[CT][GA]CC
BciVI        2105  GTATCC               GTATCC(6/5)
BglI         1264  GCCTGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1589  AGATCT               A^GATCT
BlnI          259  CCTAGG               C^CTAGG
BsaHI         796  GACGTC               G[GA]^CG[CT]C
BsgI         1900  GTGCAG               GTGCAG(16/14)
BsmI         1874  GCATTC               GAATGC(1/-1)
Bsp1407I      575  TGTACA               T^GTACA
BspHI        1883  TCATGA               T^CATGA
BspLU11I     1277  ACATGT               A^CATGT
BsrBI        2377  GAGCGG               CCGCTC(-3/-3)
BsrGI         575  TGTACA               T^GTACA
BssSI        1147  CACGAG               CACGAG(-5/-1)
BstUI         634  CGCG                 CG^CG
EarI          880  CTCTTC               CTCTTC(1/4)
FspI         2492  TGCGCA               TGC^GCA
HgaI         2295  GCGTC                GACGC(5/10)
Hin1I         796  GACGTC               G[GA]^CG[CT]C
HincII        126  GTTAAC               GT[CT]^[GA]AC
HindII        126  GTTAAC               GT[CT]^[GA]AC
HpaI          126  GTTAAC               GTT^AAC
KpnI         1697  GGTACC               GGTAC^C
Ksp632I       880  CTCTTC               CTCTTC(1/4)
MvnI          634  CGCG                 CG^CG
NruI          633  TCGCGA               TCG^CGA
RcaI         1883  TCATGA               T^CATGA
SacI         2236  GAGCTC               GAGCT^C
SbfI          732  CCTGCAGG             CCTGCA^GG
ScaI         1943  AGTACT               AGT^ACT
SmaI         1184  CCCGGG               CCC^GGG
SphI          492  GCATGC               GCATG^C
Sse8387I      732  CCTGCAGG             CCTGCA^GG
SspBI         575  TGTACA               T^GTACA
TfiI         2300  GATTC                G^A[AT]TC
XmaCI        1184  CCCGGG               C^CCGGG
XmaI         1184  CCCGGG               C^CCGGG
XmnI         1870  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII       1277  ACATGT               A^C[GA][CT]GT
             2258  ACACGT              
AvaI          224  CCCGAG               C^[CT]CG[GA]G
             1184  CCCGGG              
BclI          960  TGATCA               T^GATCA
             1938  TGATCA              
BsoBI         224  CCCGAG               C^[CT]CG[GA]G
             1184  CCCGGG              
BstEII       1231  GGTTACC              G^GT[ACGT]ACC
             1368  GGTCACC             
BstPI        1231  GGTTACC              G^GT[ACGT]ACC
             1368  GGTCACC             
EcoNI         186  CCTACTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2017  CCTGGCCAAGG         
EcoO65I      1231  GGTTACC              G^GT[ACGT]ACC
             1368  GGTCACC             
FauI         1055  CCCGC                CCCGC(4/6)
             2379  GCGGG               
FbaI          960  TGATCA               T^GATCA
             1938  TGATCA              
MaeII         797  ACGT                 A^CGT
             2260  ACGT                
MseI          127  TTAA                 T^TAA
             1178  TTAA                
PpuMI         141  GGGACCC              [GA]G^G[AT]CC[CT]
             1031  GGGACCC             
PshAI         196  GACCGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1033  GACCCCAGTC          
PstI          662  CTGCAG               CTGCA^G
              733  CTGCAG              
SfcI          662  CTGCAG               C^T[GA][CT]AG
              733  CTGCAG              
TaqI         2142  TCGA                 T^CGA
             2298  TCGA                
Tru9I         127  TTAA                 T^TAA
             1178  TTAA                
Tsp509I       550  AATT                 ^AATT
             1294  AATT                
TthHB8I      2142  TCGA                 T^CGA
             2298  TCGA                
XcmI         1740  CCACTTGGACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2055  CCAGAAGCATCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        Aor51HI     ApaI        AspHI       AvaII       
BalI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Bsu36I      Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        Eco47III    Eco81I      
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TseI        Tsp45I      TspRI       
XhoII       
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