Restriction map for pF1KSDA0088
 (Linear) (Six-base) MAPSORT of: KIAA0088.seq  Check: 247  from: 1  to: 2541



KIAA0088.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AatII G_ACGT'C

Cuts at:      0     800    2541 
   Size:        800    1741
 
AccI GT'mk_AC

Cuts at:      0    1109    2541 
   Size:       1109    1432
 
Acc65I G'GTAC_C

Cuts at:      0    1697    2541 
   Size:       1697     844
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532    2150    2366    2541 
   Size:        532    1618     216     175

  Fragments arranged by size: 

                1618     532     216     175
 
AfeI AGC'GCT

Cuts at:      0    1072    1477    1537    2541 
   Size:       1072     405      60    1004

  Fragments arranged by size: 

                1072    1004     405      60
 
AflIII A'CryG_T

Cuts at:      0    1277    2258    2541 
   Size:       1277     981     283

  Fragments arranged by size: 

                1277     981     283
 
AlwNI CAG_nnn'CTG

Cuts at:      0     784    1987    2079    2344    2413    2541 
   Size:        784    1203      92     265      69     128

  Fragments arranged by size: 

                1203     784     265     128      92      69
 
ApaI G_GGCC'C

Cuts at:      0     258     306     818    2541 
   Size:        258      48     512    1723

  Fragments arranged by size: 

                1723     512     258      48
 
ApaLI G'TGCA_C

Cuts at:      0    1444    2541 
   Size:       1444    1097
 
AvaI C'yCGr_G

Cuts at:      0     224    1184    2541 
   Size:        224     960    1357

  Fragments arranged by size: 

                1357     960     224
 
AvrII C'CTAG_G

Cuts at:      0     259    2541 
   Size:        259    2282
 
BamHI G'GATC_C

Cuts at:      0     482    2541 
   Size:        482    2059
 
BanI G'GyrC_C

Cuts at:      0    1697    2541 
   Size:       1697     844
 
BanII G_rGCy'C

Cuts at:      0     258     306     742     818    1306    1995    2240    2541 
   Size:        258      48     436      76     488     689     245     301

  Fragments arranged by size: 

                 689     488     436     301     258     245      76      48
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     709     792    2156    2541 
   Size:        709      83    1364     385

  Fragments arranged by size: 

                1364     709     385      83
 
BbvCI CC'TCA_GC

Cuts at:      0     137     290    1049    2541 
   Size:        137     153     759    1492

  Fragments arranged by size: 

                1492     759     153     137
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2116    2541 
   Size:       2116     425
 
BclI T'GATC_A

Cuts at:      0     960    1938    2541 
   Size:        960     978     603

  Fragments arranged by size: 

                 978     960     603
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1270    2541 
   Size:       1270    1271
 
BglII A'GATC_T

Cuts at:      0    1589    2541 
   Size:       1589     952
 
BlpI GC'TnA_GC

Cuts at:      0    1224    1305    2334    2541 
   Size:       1224      81    1029     207

  Fragments arranged by size: 

                1224    1029     207      81
 
Bme1580I G_kGCm'C

Cuts at:      0     258     306     818    1448    1734    2541 
   Size:        258      48     512     630     286     807

  Fragments arranged by size: 

                 807     630     512     286     258      48
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1031    1760    2541 
   Size:       1031     729     781

  Fragments arranged by size: 

                1031     781     729
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46    2009    2313    2407    2423    2541 
   Size:         46    1963     304      94      16     118

  Fragments arranged by size: 

                1963     304     118      94      46      16
 
Bpu10I CC'TnA_GC

Cuts at:      0     137     290     488    1049    2541 
   Size:        137     153     198     561    1492

  Fragments arranged by size: 

                1492     561     198     153     137
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1363    2213    2541 
   Size:       1087     276     850     328

  Fragments arranged by size: 

                1087     850     328     276
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     270     661     742    2467    2541 
   Size:        270     391      81    1725      74

  Fragments arranged by size: 

                1725     391     270      81      74
 
BsaHI Gr'CG_yC

Cuts at:      0     797    2541 
   Size:        797    1744
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2020    2050    2541 
   Size:       2020      30     491

  Fragments arranged by size: 

                2020     491      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     403    1165    2541 
   Size:        358      45     762    1376

  Fragments arranged by size: 

                1376     762     358      45
 
BseYI C'CCAG_C

Cuts at:      0     285     305     810    1388    1403    1532    2541 
   Size:        285      20     505     578      15     129    1009

  Fragments arranged by size: 

                1009     578     505     285     129      20      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1921    2541 
   Size:       1921     620
 
BsiHKAI G_wGCw'C

Cuts at:      0     614    1412    1448    2240    2541 
   Size:        614     798      36     792     301

  Fragments arranged by size: 

                 798     792     614     301      36
 
BsmI GAATG_Cn'

Cuts at:      0    1874    2541 
   Size:       1874     667
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     306     614     742     818    1306    1412    1448 
   Size:        258      48     308     128      76     488     106      36

Cuts at:   1448    1734    1995    2240    2541 
   Size:        286     261     245     301

  Fragments arranged by size: 

                 488     308     301     286     261     258     245     128
                 106      76      48      36
 
BspHI T'CATG_A

Cuts at:      0    1883    2541 
   Size:       1883     658
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     740     977    2093    2541 
   Size:        740     237    1116     448

  Fragments arranged by size: 

                1116     740     448     237
 
BsrBI CCG'CTC

Cuts at:      0    2379    2541 
   Size:       2379     162
 
BsrGI T'GTAC_A

Cuts at:      0     575    2541 
   Size:        575    1966
 
BssSI C'ACGA_G

Cuts at:      0    1147    2541 
   Size:       1147    1394
 
BstEII G'GTnAC_C

Cuts at:      0    1231    1368    2541 
   Size:       1231     137    1173

  Fragments arranged by size: 

                1231    1173     137
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     383     438    1396    1540    2541 
   Size:        383      55     958     144    1001

  Fragments arranged by size: 

                1001     958     383     144      55
 
BstYI r'GATC_y

Cuts at:      0     482    1589    2434    2541 
   Size:        482    1107     845     107

  Fragments arranged by size: 

                1107     845     482     107
 
Bsu36I CC'TnA_GG

Cuts at:      0     311     505    1364    1910    2216    2541 
   Size:        311     194     859     546     306     325

  Fragments arranged by size: 

                 859     546     325     311     306     194
 
BtgI C'CryG_G

Cuts at:      0     131    1765    1995    2541 
   Size:        131    1634     230     546

  Fragments arranged by size: 

                1634     546     230     131
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     311     616    2541 
   Size:        311     305    1925

  Fragments arranged by size: 

                1925     311     305
 
BtsI GCAGTG_nn'

Cuts at:      0     692    1565    2541 
   Size:        692     873     976

  Fragments arranged by size: 

                 976     873     692
 
EaeI y'GGCC_r

Cuts at:      0     169    1100    1216    1508    2019    2541 
   Size:        169     931     116     292     511     522

  Fragments arranged by size: 

                 931     522     511     292     169     116
 
EarI CTCTTCn'nnn_

Cuts at:      0     886    2541 
   Size:        886    1655
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     166    1104    1660    2541 
   Size:        166     938     556     881

  Fragments arranged by size: 

                 938     881     556     166
 
EcoICRI GAG'CTC

Cuts at:      0    2238    2541 
   Size:       2238     303
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     532    2009    2150    2313    2366    2407    2423 
   Size:         46     486    1477     141     163      53      41      16

Cuts at:   2423    2541 
   Size:        118

  Fragments arranged by size: 

                1477     486     163     141     118      53      46      41
                  16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     190    2021    2541 
   Size:        190    1831     520

  Fragments arranged by size: 

                1831     520     190
 
EcoO109I rG'GnC_Cy

Cuts at:      0     142     254     255     302     814     815    1032    1262 
   Size:        142     112       1      47     512       1     217     230

Cuts at:   1262    1514    1889    2541 
   Size:        252     375     652

  Fragments arranged by size: 

                 652     512     375     252     230     217     142     112
                  47       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     174    2541 
   Size:        174    2367
 
FspI TGC'GCA

Cuts at:      0    2494    2541 
   Size:       2494      47
 
HaeII r_GCGC'y

Cuts at:      0    1074    1479    1539    2541 
   Size:       1074     405      60    1002

  Fragments arranged by size: 

                1074    1002     405      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2117    2149    2541 
   Size:       2117      32     392

  Fragments arranged by size: 

                2117     392      32
 
HincII GTy'rAC

Cuts at:      0     128    2541 
   Size:        128    2413
 
HpaI GTT'AAC

Cuts at:      0     128    2541 
   Size:        128    2413
 
KpnI G_GTAC'C

Cuts at:      0    1701    2541 
   Size:       1701     840
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     713     889    2541 
   Size:        713     176    1652

  Fragments arranged by size: 

                1652     713     176
 
MscI TGG'CCA

Cuts at:      0    1102    1218    1510    2021    2541 
   Size:       1102     116     292     511     520

  Fragments arranged by size: 

                1102     520     511     292     116
 
MslI CAynn'nnrTG

Cuts at:      0     436     476    1007    1123    1415    2509    2541 
   Size:        436      40     531     116     292    1094      32

  Fragments arranged by size: 

                1094     531     436     292     116      40      32
 
MspA1I CmG'CkG

Cuts at:      0     289    1228    2413    2541 
   Size:        289     939    1185     128

  Fragments arranged by size: 

                1185     939     289     128
 
NcoI C'CATG_G

Cuts at:      0     131    1765    1995    2541 
   Size:        131    1634     230     546

  Fragments arranged by size: 

                1634     546     230     131
 
NruI TCG'CGA

Cuts at:      0     635    2541 
   Size:        635    1906
 
NspI r_CATG'y

Cuts at:      0     475     496    1006    1281    1738    2541 
   Size:        475      21     510     275     457     803

  Fragments arranged by size: 

                 803     510     475     457     275      21
 
PasI CC'CwG_GG

Cuts at:      0     323    2541 
   Size:        323    2218
 
PciI A'CATG_T

Cuts at:      0    1277    2541 
   Size:       1277    1264
 
PpuMI rG'GwC_Cy

Cuts at:      0     142    1032    2541 
   Size:        142     890    1509

  Fragments arranged by size: 

                1509     890     142
 
PshAI GACnn'nnGTC

Cuts at:      0     200    1037    2541 
   Size:        200     837    1504

  Fragments arranged by size: 

                1504     837     200
 
PspOMI G'GGCC_C

Cuts at:      0     254     302     814    2541 
   Size:        254      48     512    1727

  Fragments arranged by size: 

                1727     512     254      48
 
PstI C_TGCA'G

Cuts at:      0     666     737    2541 
   Size:        666      71    1804

  Fragments arranged by size: 

                1804     666      71
 
PvuII CAG'CTG

Cuts at:      0     289    1228    2413    2541 
   Size:        289     939    1185     128

  Fragments arranged by size: 

                1185     939     289     128
 
SacI G_AGCT'C

Cuts at:      0    2240    2541 
   Size:       2240     301
 
SanDI GG'GwC_CC

Cuts at:      0     142    1032    2541 
   Size:        142     890    1509

  Fragments arranged by size: 

                1509     890     142
 
SbfI CC_TGCA'GG

Cuts at:      0     737    2541 
   Size:        737    1804
 
ScaI AGT'ACT

Cuts at:      0    1945    2541 
   Size:       1945     596
 
SfcI C'TryA_G

Cuts at:      0     662     733    2541 
   Size:        662      71    1808

  Fragments arranged by size: 

                1808     662      71
 
SmaI CCC'GGG

Cuts at:      0    1186    2541 
   Size:       1186    1355
 
SmlI C'TyrA_G

Cuts at:      0    1066    1378    2228    2541 
   Size:       1066     312     850     313

  Fragments arranged by size: 

                1066     850     313     312
 
SphI G_CATG'C

Cuts at:      0     496    2541 
   Size:        496    2045
 
StyI C'CwwG_G

Cuts at:      0     131     259    1765    1808    1995    2022    2541 
   Size:        131     128    1506      43     187      27     519

  Fragments arranged by size: 

                1506     519     187     131     128      43      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     212     336    1642    1696    1887    2541 
   Size:        212     124    1306      54     191     654

  Fragments arranged by size: 

                1306     654     212     191     124      54
 
TatI w'GTAC_w

Cuts at:      0     575    1943    2541 
   Size:        575    1368     598

  Fragments arranged by size: 

                1368     598     575
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190    2541 
   Size:        158      32    2351

  Fragments arranged by size: 

                2351     158      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1747    2062    2541 
   Size:       1747     315     479

  Fragments arranged by size: 

                1747     479     315
 
XmaI C'CCGG_G

Cuts at:      0    1184    2541 
   Size:       1184    1357
 
XmnI GAAnn'nnTTC

Cuts at:      0    1874    2541 
   Size:       1874     667
 
ZraI GAC'GTC

Cuts at:      0     798    2541 
   Size:        798    1743
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI   AflIII    AlwNI     ApaI
    ApaLI     AvaI    AvrII    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrGI    BssSI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII     HpaI     KpnI     MmeI     MscI     MslI
   MspA1I     NcoI     NruI     NspI     PasI     PciI    PpuMI    PshAI
   PspOMI     PstI    PvuII     SacI    SanDI     SbfI     ScaI     SfcI
     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI     BaeI     BbeI     BcgI    BfrBI
    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BsrDI    BsrFI   BssHII   BstAPI    BstBI  BstZ17I
     ClaI    CspCI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV
     FseI    FspAI  HindIII     KasI     MfeI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NsiI     PacI    PflMI     PfoI
     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XhoI
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