Restriction map for pF1KSDA0076
##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       BamHI       BbeI        
BfrI        BsiWI       Bsp1407I    BspDI       BsrBI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRV       EcoT22I     FseI        FspI        HindIII     
HpaI        KasI        KpnI        MfeI        MluI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SwaI        Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        4858  GACGTC               GACGT^C
AccIII       2025  TCCGGA               T^CCGGA
AflIII        503  ACATGT               A^C[GA][CT]GT
Alw44I       1267  GTGCAC               G^TGCAC
Aor51HI      2849  AGCGCT               AGC^GCT
ApaLI        1267  GTGCAC               G^TGCAC
AvrII        2898  CCTAGG               C^CTAGG
BciVI        4002  GGATAC               GTATCC(6/5)
BclI         1676  TGATCA               T^GATCA
BlnI         2898  CCTAGG               C^CTAGG
BsaBI          97  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2025  TCCGGA               [AT]^CCGG[AT]
BseAI        2025  TCCGGA               T^CCGGA
BsiEI        4914  CGACCG               CG[GA][CT]^CG
BspEI        2025  TCCGGA               T^CCGGA
BspHI        3416  TCATGA               T^CATGA
BspLU11I      503  ACATGT               A^CATGT
CpoI         3818  CGGACCG              CG^G[AT]CCG
Eco47III     2849  AGCGCT               AGC^GCT
EcoRI          25  GAATTC               G^AATTC
FbaI         1676  TGATCA               T^GATCA
KspI         3252  CCGCGG               CCGC^GG
MamI           97  GATGGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2025  TCCGGA               T^CCGGA
MseI         3527  TTAA                 T^TAA
NheI          663  GCTAGC               G^CTAGC
RcaI         3416  TCATGA               T^CATGA
RsrII        3818  CGGACCG              CG^G[AT]CCG
SacII        3252  CCGCGG               CCGC^GG
SapI          747  GCTCTTC              GCTCTTC(1/4)
SspI         3529  AATATT               AAT^ATT
Tru9I        3527  TTAA                 T^TAA
XcmI         1352  CCAGTAGAGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1690  GTCTAC               GT^[AC][GT]AC
             3958  GTCTAC              
AcsI           25  GAATTC               [GA]^AATT[CT]
             3066  GAATTT              
AcyI         4858  GACGTC               G[GA]^CG[CT]C
             4904  GACGCC              
ApoI           25  GAATTC               [GA]^AATT[CT]
             3066  GAATTT              
BbrPI         424  CACGTG               CAC^GTG
             3643  CACGTG              
BglII         218  AGATCT               A^GATCT
             1286  AGATCT              
BsaAI         424  CACGTG               [CT]AC^GT[GA]
             3643  CACGTG              
BsaHI        4858  GACGTC               G[GA]^CG[CT]C
             4904  GACGCC              
BsaI         3837  GGTCTC               GGTCTC(1/5)
             4443  GGTCTC              
BsmI          716  GCATTC               GAATGC(1/-1)
             2879  GCATTC              
BsrDI        3738  GCAATG               GCAATG(2/0)
             4153  GCAATG              
BssSI        2113  CACGAG               CACGAG(-5/-1)
             4543  CACGAG              
DrdI          475  GACCCAAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4756  GACCAGCTTGTC        
Hin1I        4858  GACGTC               G[GA]^CG[CT]C
             4904  GACGCC              
HincII        869  GTCAAC               GT[CT]^[GA]AC
             1245  GTCAAC              
HindII        869  GTCAAC               GT[CT]^[GA]AC
             1245  GTCAAC              
NaeI         2611  GCCGGC               GCC^GGC
             2703  GCCGGC              
NcoI          896  CCATGG               C^CATGG
             4731  CCATGG              
NgoMIV       2611  GCCGGC               G^CCGGC
             2703  GCCGGC              
PaeR7I       2691  CTCGAG               C^TCGAG
             3840  CTCGAG              
PmaCI         424  CACGTG               CAC^GTG
             3643  CACGTG              
PmlI          424  CACGTG               CAC^GTG
             3643  CACGTG              
SacI          963  GAGCTC               GAGCT^C
             3381  GAGCTC              
SmaI         3395  CCCGGG               CCC^GGG
             5039  CCCGGG              
SphI         2343  GCATGC               GCATG^C
             4843  GCATGC              
StuI          944  AGGCCT               AGG^CCT
             2981  AGGCCT              
XhoI         2691  CTCGAG               C^TCGAG
             3840  CTCGAG              
XmaCI        3395  CCCGGG               C^CCGGG
             5039  CCCGGG              
XmaI         3395  CCCGGG               C^CCGGG
             5039  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XbaI        
XhoII       
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