Restriction map for pF1KSDA0074
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AviII       AvrII       BalI        BamHI       
BbrPI       BbvCI       BciVI       BclI        BfaI        
BglI        BlnI        BsaAI       BsaBI       BsaI        
BsiEI       BsiHKAI     BsiWI       Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
Bsu36I      Cfr10I      ClaI        CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccIII       1975  TCCGGA               T^CCGGA
AcsI         1895  AAATTC               [GA]^AATT[CT]
AcyI          123  GGCGCC               G[GA]^CG[CT]C
AflII        1591  CTTAAG               C^TTAAG
AlwNI        1170  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1968  AGCGCT               AGC^GCT
ApoI         1895  AAATTC               [GA]^AATT[CT]
Asp700I       157  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI          123  GGCGCC               GGCGC^C
BfrI         1591  CTTAAG               C^TTAAG
BsaHI         123  GGCGCC               G[GA]^CG[CT]C
BsaWI        1975  TCCGGA               [AT]^CCGG[AT]
BseAI        1975  TCCGGA               T^CCGGA
BsmBI          52  GAGACG               CGTCTC(1/5)
BsmI          378  GAATGC               GAATGC(1/-1)
BspEI        1975  TCCGGA               T^CCGGA
BspHI        1158  TCATGA               T^CATGA
BspMI        2078  ACCTGC               ACCTGC(4/8)
BsrBI         187  GAGCGG               CCGCTC(-3/-3)
Bst1107I      526  GTATAC               GTA^TAC
BstZ17I       526  GTATAC               GTA^TAC
DrdI         1549  GACACTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          851  CCACGG               C^C[GA][CT]GG
EaeI          956  TGGCCG               [CT]^GGCC[GA]
Eco47III     1968  AGCGCT               AGC^GCT
EcoNI         263  CCTCCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I         123  GGCGCC               G[GA]^CG[CT]C
KasI          123  GGCGCC               G^GCGCC
MroI         1975  TCCGGA               T^CCGGA
MspA1I        196  CAGCGG               C[AC]G^C[GT]G
NarI          123  GGCGCC               GG^CGCC
NdeI         1844  CATATG               CA^TATG
NspI         1949  GCATGT               [GA]CATG^[CT]
RcaI         1158  TCATGA               T^CATGA
SfoI          123  GGCGCC               GGC^GCC
StuI         1342  AGGCCT               AGG^CCT
XmnI          157  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI          526  GTATAC               GT^[AC][GT]AC
             1018  GTAGAC              
AccII          56  CGCG                 CG^CG
              502  CGCG                
AfaI          467  GTAC                 GT^AC
             1394  GTAC                
AspI         1302  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1479  GACCAAGTC           
AvaI          207  CTCGAG               C^[CT]CG[GA]G
              865  CTCGAG              
BanII        1183  GAGCCC               G[GA]GC[CT]^C
             2065  GGGCTC              
BcgI          104  CGATGCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2072  CGAAGGACCTGC        
BglII         488  AGATCT               A^GATCT
              941  AGATCT              
BlpI         1867  GCTGAGC              GC^T[ACGT]AGC
             2058  GCTTAGC             
Bpu1102I     1867  GCTGAGC              GC^T[ACGT]AGC
             2058  GCTTAGC             
BseRI          78  CTCCTC               GAGGAG(10/8)
             2085  GAGGAG              
BsgI          762  GTGCAG               GTGCAG(16/14)
              926  GTGCAG              
BsoBI         207  CTCGAG               C^[CT]CG[GA]G
              865  CTCGAG              
BstUI          56  CGCG                 CG^CG
              502  CGCG                
BstXI        1916  CCAAAAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2120  CCATACCTCTGG        
BstYI         488  AGATCT               [GA]^GATC[CT]
              941  AGATCT              
CelII        1867  GCTGAGC              GC^T[ACGT]AGC
             2058  GCTTAGC             
DraI          451  TTTAAA               TTT^AAA
              903  TTTAAA              
FauI          463  GCGGG                CCCGC(4/6)
             2063  GCGGG               
HgaI           54  GACGC                GACGC(5/10)
             1349  GACGC               
HincII       1066  GTTGAC               GT[CT]^[GA]AC
             1546  GTTGAC              
HindII       1066  GTTGAC               GT[CT]^[GA]AC
             1546  GTTGAC              
MflI          488  AGATCT               [GA]^GATC[CT]
              941  AGATCT              
MslI          517  CATGCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1305  CATGTCGATG          
MvnI           56  CGCG                 CG^CG
              502  CGCG                
PaeR7I        207  CTCGAG               C^TCGAG
              865  CTCGAG              
PflFI        1302  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1479  GACCAAGTC           
RsaI          467  GTAC                 GT^AC
             1394  GTAC                
SexAI        1040  ACCAGGT              A^CC[AT]GGT
             1582  ACCAGGT             
SspI          133  AATATT               AAT^ATT
             1280  AATATT              
TfiI          417  GATTC                G^A[AT]TC
             2166  GAATC               
Tsp45I       1076  GTGAC                ^GT[GC]AC
             1808  GTGAC               
Tth111I      1302  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1479  GACCAAGTC           
XhoI          207  CTCGAG               C^TCGAG
              865  CTCGAG              
XhoII         488  AGATCT               [GA]^GATC[CT]
              941  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanI        
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XcmI        
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