Restriction map for pF1KSDA0074
 (Linear) (Six-base) MAPSORT of: KIAA0074.seq  Check: 9772  from: 1  to: 2223



KIAA0074.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0     527    1019    2223 
   Size:        527     492    1204

  Fragments arranged by size: 

                1204     527     492
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      71    1082    1960    2223 
   Size:         32      39    1011     878     263

  Fragments arranged by size: 

                1011     878     263      39      32
 
AfeI AGC'GCT

Cuts at:      0    1970    2223 
   Size:       1970     253
 
AflII C'TTAA_G

Cuts at:      0    1591    2223 
   Size:       1591     632
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     941    2223 
   Size:        941    1282
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1175    2223 
   Size:       1175    1048
 
ApoI r'AATT_y

Cuts at:      0    1895    2223 
   Size:       1895     328
 
AvaI C'yCGr_G

Cuts at:      0     207     865    2223 
   Size:        207     658    1358

  Fragments arranged by size: 

                1358     658     207
 
BanI G'GyrC_C

Cuts at:      0     123     142    1579    2223 
   Size:        123      19    1437     644

  Fragments arranged by size: 

                1437     644     123      19
 
BanII G_rGCy'C

Cuts at:      0    1187    2069    2223 
   Size:       1187     882     154

  Fragments arranged by size: 

                1187     882     154
 
BbeI G_GCGC'C

Cuts at:      0     127    2223 
   Size:        127    2096
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     164     745    1649    2223 
   Size:        164     581     904     574

  Fragments arranged by size: 

                 904     581     574     164
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     127    2061    2095    2223 
   Size:         93      34    1934      34     128

  Fragments arranged by size: 

                1934     128      93      34      34
 
BglII A'GATC_T

Cuts at:      0     488     941    2223 
   Size:        488     453    1282

  Fragments arranged by size: 

                1282     488     453
 
BlpI GC'TnA_GC

Cuts at:      0    1868    2059    2223 
   Size:       1868     191     164

  Fragments arranged by size: 

                1868     191     164
 
Bme1580I G_kGCm'C

Cuts at:      0    1255    2223 
   Size:       1255     968
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     142    2223 
   Size:        142    2081
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     880    2211    2223 
   Size:        880    1331      12

  Fragments arranged by size: 

                1331     880      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249    1295    1799    2223 
   Size:        249    1046     504     424

  Fragments arranged by size: 

                1046     504     424     249
 
BsaHI Gr'CG_yC

Cuts at:      0     124    2223 
   Size:        124    2099
 
BsaWI w'CCGG_w

Cuts at:      0    1975    2223 
   Size:       1975     248
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91    1657    1687    2223 
   Size:         61      30    1566      30     536

  Fragments arranged by size: 

                1566     536      61      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69    2100    2223 
   Size:         69    2031     123

  Fragments arranged by size: 

                2031     123      69
 
BseYI C'CCAG_C

Cuts at:      0      15     546    2223 
   Size:         15     531    1677

  Fragments arranged by size: 

                1677     531      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     783     947    2223 
   Size:        783     164    1276

  Fragments arranged by size: 

                1276     783     164
 
BsmI GAATG_Cn'

Cuts at:      0     384    2223 
   Size:        384    1839
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      46    2223 
   Size:         46    2177
 
Bsp1286I G_dGCh'C

Cuts at:      0    1187    1255    2069    2223 
   Size:       1187      68     814     154

  Fragments arranged by size: 

                1187     814     154      68
 
BspEI T'CCGG_A

Cuts at:      0    1975    2223 
   Size:       1975     248
 
BspHI T'CATG_A

Cuts at:      0    1158    2223 
   Size:       1158    1065
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2087    2223 
   Size:       2087     136
 
BsrBI CCG'CTC

Cuts at:      0     189    2223 
   Size:        189    2034
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1923    2127    2223 
   Size:       1923     204      96

  Fragments arranged by size: 

                1923     204      96
 
BstYI r'GATC_y

Cuts at:      0     488     941    2223 
   Size:        488     453    1282

  Fragments arranged by size: 

                1282     488     453
 
BstZ17I GTA'TAC

Cuts at:      0     528    2223 
   Size:        528    1695
 
BtgI C'CryG_G

Cuts at:      0     851    2223 
   Size:        851    1372
 
BtsI GCAGTG_nn'

Cuts at:      0      19     795     928    2223 
   Size:         19     776     133    1295

  Fragments arranged by size: 

                1295     776     133      19
 
DraI TTT'AAA

Cuts at:      0     453     905    2223 
   Size:        453     452    1318

  Fragments arranged by size: 

                1318     453     452
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1555    2223 
   Size:       1555     668
 
EaeI y'GGCC_r

Cuts at:      0     956    2223 
   Size:        956    1267
 
EarI CTCTTCn'nnn_

Cuts at:      0      48    1157    1384    2223 
   Size:         48    1109     227     839

  Fragments arranged by size: 

                1109     839     227      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      71     249    1082    1295    1799    1960    2223 
   Size:         32      39     178     833     213     504     161     263

  Fragments arranged by size: 

                 833     504     263     213     178     161      39      32
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     267    2223 
   Size:        267    1956
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4    1100    1741    2076    2190    2223 
   Size:          4    1096     641     335     114      33

  Fragments arranged by size: 

                1096     641     335     114      33       4
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     669    2223 
   Size:        669    1554
 
HaeII r_GCGC'y

Cuts at:      0     127     207     913    1338    1972    2223 
   Size:        127      80     706     425     634     251

  Fragments arranged by size: 

                 706     634     425     251     127      80
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     394     426     547     579     991    1023    1820    1852 
   Size:        394      32     121      32     412      32     797      32

Cuts at:   1852    2223 
   Size:        371

  Fragments arranged by size: 

                 797     412     394     371     121      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1068    1548    2223 
   Size:       1068     480     675

  Fragments arranged by size: 

                1068     675     480
 
KasI G'GCGC_C

Cuts at:      0     123    2223 
   Size:        123    2100
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1700    2223 
   Size:       1700     523
 
MslI CAynn'nnrTG

Cuts at:      0     521    1309    2223 
   Size:        521     788     914

  Fragments arranged by size: 

                 914     788     521
 
MspA1I CmG'CkG

Cuts at:      0     198    2223 
   Size:        198    2025
 
NarI GG'CG_CC

Cuts at:      0     124    2223 
   Size:        124    2099
 
NdeI CA'TA_TG

Cuts at:      0    1845    2223 
   Size:       1845     378
 
NspI r_CATG'y

Cuts at:      0    1953    2223 
   Size:       1953     270
 
PasI CC'CwG_GG

Cuts at:      0    1104    1602    2223 
   Size:       1104     498     621

  Fragments arranged by size: 

                1104     621     498
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114    2223 
   Size:         82      32    2109

  Fragments arranged by size: 

                2109      82      32
 
PpuMI rG'GwC_Cy

Cuts at:      0       4    1100    1741    2076    2190    2223 
   Size:          4    1096     641     335     114      33

  Fragments arranged by size: 

                1096     641     335     114      33       4
 
PspXI vC'TCGA_Gb

Cuts at:      0     207    2223 
   Size:        207    2016
 
PstI C_TGCA'G

Cuts at:      0     197     227    2084    2223 
   Size:        197      30    1857     139

  Fragments arranged by size: 

                1857     197     139      30
 
SanDI GG'GwC_CC

Cuts at:      0    1100    2223 
   Size:       1100    1123
 
SexAI A'CCwGG_T

Cuts at:      0    1040    1582    2223 
   Size:       1040     542     641

  Fragments arranged by size: 

                1040     641     542
 
SfcI C'TryA_G

Cuts at:      0     193     223     807    1526    2080    2223 
   Size:        193      30     584     719     554     143

  Fragments arranged by size: 

                 719     584     554     193     143      30
 
SfoI GGC'GCC

Cuts at:      0     125    2223 
   Size:        125    2098
 
SmlI C'TyrA_G

Cuts at:      0     207     865    1591    2223 
   Size:        207     658     726     632

  Fragments arranged by size: 

                 726     658     632     207
 
SspI AAT'ATT

Cuts at:      0     135    1282    2223 
   Size:        135    1147     941

  Fragments arranged by size: 

                1147     941     135
 
StuI AGG'CCT

Cuts at:      0    1344    2223 
   Size:       1344     879
 
StyI C'CwwG_G

Cuts at:      0     537    1224    1824    2223 
   Size:        537     687     600     399

  Fragments arranged by size: 

                 687     600     537     399
 
TatI w'GTAC_w

Cuts at:      0    1393    2223 
   Size:       1393     830
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      61      93    2223 
   Size:         61      32    2130

  Fragments arranged by size: 

                2130      61      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1305    1482    2223 
   Size:       1305     177     741

  Fragments arranged by size: 

                1305     741     177
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     453     592    1147    1488    2011    2223 
   Size:        453     139     555     341     523     212

  Fragments arranged by size: 

                 555     523     453     341     212     139
 
XhoI C'TCGA_G

Cuts at:      0     207     865    2223 
   Size:        207     658    1358

  Fragments arranged by size: 

                1358     658     207
 
XmnI GAAnn'nnTTC

Cuts at:      0     161    2223 
   Size:        161    2062
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII     AlfI    AlwNI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI     BcgI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BstXI    BstYI  BstZ17I     BtgI     BtsI     DraI     DrdI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII
     KasI     MmeI     MslI   MspA1I     NarI     NdeI     NspI     PasI
     PpiI    PpuMI    PspXI     PstI    SanDI    SexAI     SfcI     SfoI
     SmlI     SspI     StuI     StyI     TatI     TstI  Tth111I     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsiEI  BsiHKAI    BsiWI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     NaeI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII     XbaI
     XmaI     ZraI
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