Restriction map for pF1KSDA0063
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        561  GGTACC               G^GTACC
AfaI          562  GTAC                 GT^AC
AlwI          419  GGATC                GGATC(4/5)
Asp718I       561  GGTACC               G^GTACC
AspHI         502  GAGCTC               G[AT]GC[AT]^C
BanI          561  GGTACC               G^G[CT][GA]CC
BanII         502  GAGCTC               G[GA]GC[CT]^C
BbsI          130  GTCTTC               GAAGAC(2/6)
BcgI          311  GCAGGGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         159  GGATAC               GTATCC(6/5)
BpmI          369  CTCCAG               CTGGAG(16/14)
BpuAI         130  GTCTTC               GAAGAC(2/6)
BsiHKAI       502  GAGCTC               G[AT]GC[AT]^C
BsmFI         302  GGGAC                GGGAC(10/14)
BspHI         356  TCATGA               T^CATGA
BsrI          416  ACTGG                ACTGG(1/-1)
BstF5I        315  GGATG                GGATG(2/0)
BstYI         419  GGATCT               [GA]^GATC[CT]
DdeI          505  CTCAG                C^T[ACGT]AG
DpnII         420  GATC                 ^GATC
DraII         442  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      442  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FokI          315  GGATG                GGATG(9/13)
HapII         156  CCGG                 C^CGG
HpaII         156  CCGG                 C^CGG
KpnI          561  GGTACC               GGTAC^C
MaeIII        205  GTGAC                ^GT[ACGT]AC
MboI          420  GATC                 ^GATC
MflI          419  GGATCT               [GA]^GATC[CT]
MslI          198  CACCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          156  CCGG                 C^CGG
NdeII         420  GATC                 ^GATC
NspI          224  GCATGC               [GA]CATG^[CT]
PleI          460  GACTC                GAGTC(4/5)
RcaI          356  TCATGA               T^CATGA
RsaI          562  GTAC                 GT^AC
SacI          502  GAGCTC               GAGCT^C
SapI          219  GAAGAGC              GCTCTTC(1/4)
Sau3AI        420  GATC                 ^GATC
SfaNI         474  GATGC                GCATC(5/9)
SmaI          155  CCCGGG               CCC^GGG
SmlI          469  CTCAAG               C^T[CT][GA]AG
SphI          224  GCATGC               GCATG^C
Tsp45I        205  GTGAC                ^GT[GC]AC
XhoII         419  GGATCT               [GA]^GATC[CT]
XmaCI         155  CCCGGG               C^CCGGG
XmaI          155  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AvaI          155  CCCGGG               C^[CT]CG[GA]G
              478  CCCGAG              
AvaII         385  GGTCC                G^G[AT]CC
              596  GGACC               
BcnI          155  CCCGG                CC^[GC]GG
              156  CCGGG               
BmyI          107  GTGCCC               G[AGT]GC[ACT]^C
              502  GAGCTC              
BsmAI          23  GAGAC                GTCTC(1/5)
              189  GTCTC               
BsoBI         155  CCCGGG               C^[CT]CG[GA]G
              478  CCCGAG              
Bsp1286I      107  GTGCCC               G[AGT]GC[ACT]^C
              502  GAGCTC              
BsrDI         327  CATTGC               GCAATG(2/0)
              143  GCAATG              
BstNI          57  CCAGG                CC^[AT]GG
              135  CCAGG               
Cac8I         224  GCATGC               GC[ACGT]^[ACGT]GC
              500  GCGAGC              
CfoI           93  GCGC                 GCG^C
              309  GCGC                
DsaI           13  CCATGG               C^C[GA][CT]GG
              200  CCATGG              
EarI          219  GAAGAG               CTCTTC(1/4)
              568  GAAGAG              
EcoRII         57  CCAGG                ^CC[AT]GG
              135  CCAGG               
EcoT14I        13  CCATGG               C^C[AT][AT]GG
              200  CCATGG              
HaeIII         30  GGCC                 GG^CC
              444  GGCC                
HhaI           93  GCGC                 GCG^C
              309  GCGC                
HinP1I         93  GCGC                 G^CGC
              309  GCGC                
HphI          204  GGTGA                GGTGA(8/7)
              152  TCACC               
Ksp632I       219  GAAGAG               CTCTTC(1/4)
              568  GAAGAG              
MaeII         128  ACGT                 A^CGT
              341  ACGT                
MvaI           57  CCAGG                CC^[AT]GG
              135  CCAGG               
MwoI           93  GCGCAGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              109  GCCCTCCACGC         
NciI          155  CCCGG                CC^[GC]GG
              156  CCGGG               
NcoI           13  CCATGG               C^CATGG
              200  CCATGG              
StyI           13  CCATGG               C^C[AT][AT]GG
              200  CCATGG              
TaqI          429  TCGA                 T^CGA
              458  TCGA                
TfiI           40  GAATC                G^A[AT]TC
              360  GAATC               
TspRI         389  CACTG                CA[GC]TG[ACGT][ACGT]^
              415  CACTG               
TthHB8I       429  TCGA                 T^CGA
              458  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BbvI        BsaJI       BsiYI       BslI        
BssKI       Cfr13I      Fnu4HI      HinfI       ItaI        
MboII       MnlI        NlaIII      NlaIV       Sau96I      
ScrFI       TseI        
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