Restriction map for pF1KSDA0057
 (Linear) (Six-base) MAPSORT of: KIAA0057.seq  Check: 6513  from: 1  to: 1110



KIAA0057.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21    1110 
   Size:         21    1089
 
AhdI GACnn_n'nnGTC

Cuts at:      0     358    1110 
   Size:        358     752
 
AleI CACnn'nnGTG

Cuts at:      0     379    1110 
   Size:        379     731
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1063    1095    1110 
   Size:       1063      32      15

  Fragments arranged by size: 

                1063      32      15
 
AlwNI CAG_nnn'CTG

Cuts at:      0     740     878    1110 
   Size:        740     138     232

  Fragments arranged by size: 

                 740     232     138
 
ApaLI G'TGCA_C

Cuts at:      0     196     460    1110 
   Size:        196     264     650

  Fragments arranged by size: 

                 650     264     196
 
ApoI r'AATT_y

Cuts at:      0     560    1110 
   Size:        560     550
 
AvaI C'yCGr_G

Cuts at:      0     841    1110 
   Size:        841     269
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     452    1110 
   Size:        452     658
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     409    1110 
   Size:        409     701
 
BglI GCCn_nnn'nGGC

Cuts at:      0     638     793     907    1110 
   Size:        638     155     114     203

  Fragments arranged by size: 

                 638     203     155     114
 
Bme1580I G_kGCm'C

Cuts at:      0     200     464    1110 
   Size:        200     264     646

  Fragments arranged by size: 

                 646     264     200
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     634    1110 
   Size:        634     476
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     629    1110 
   Size:        559      70     481

  Fragments arranged by size: 

                 559     481      70
 
Bpu10I CC'TnA_GC

Cuts at:      0     527     628    1110 
   Size:        527     101     482

  Fragments arranged by size: 

                 527     482     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1110 
   Size:       1084      26
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     184    1110 
   Size:        184     926
 
BsaAI yAC'GTr

Cuts at:      0     399    1110 
   Size:        399     711
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1063    1093    1110 
   Size:       1063      30      17

  Fragments arranged by size: 

                1063      30      17
 
BseYI C'CCAG_C

Cuts at:      0     938     993    1110 
   Size:        938      55     117

  Fragments arranged by size: 

                 938     117      55
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1110 
   Size:        499     611
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     200     464    1110 
   Size:         92     108     264     646

  Fragments arranged by size: 

                 646     264     108      92
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     200     464    1110 
   Size:         92     108     264     646

  Fragments arranged by size: 

                 646     264     108      92
 
BsrBI CCG'CTC

Cuts at:      0      33    1110 
   Size:         33    1077
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     878    1110 
   Size:        878     232
 
BstEII G'GTnAC_C

Cuts at:      0     759    1039    1110 
   Size:        759     280      71

  Fragments arranged by size: 

                 759     280      71
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1051    1110 
   Size:       1051      59
 
Bsu36I CC'TnA_GG

Cuts at:      0     215    1110 
   Size:        215     895
 
BtgI C'CryG_G

Cuts at:      0    1084    1110 
   Size:       1084      26
 
EaeI y'GGCC_r

Cuts at:      0     794    1110 
   Size:        794     316
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     685     703     775    1110 
   Size:         40     645      18      72     335

  Fragments arranged by size: 

                 645     335      72      40      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     559     629    1110 
   Size:         21     538      70     481

  Fragments arranged by size: 

                 538     481      70      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     219    1110 
   Size:        219     891
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     153    1110 
   Size:        153     957
 
HaeII r_GCGC'y

Cuts at:      0     927    1110 
   Size:        927     183
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     344     376    1110 
   Size:        344      32     734

  Fragments arranged by size: 

                 734     344      32
 
MscI TGG'CCA

Cuts at:      0     796    1110 
   Size:        796     314
 
MslI CAynn'nnrTG

Cuts at:      0      62     169     379    1049    1110 
   Size:         62     107     210     670      61

  Fragments arranged by size: 

                 670     210     107      62      61
 
MspA1I CmG'CkG

Cuts at:      0     357     501     942    1110 
   Size:        357     144     441     168

  Fragments arranged by size: 

                 441     357     168     144
 
NspI r_CATG'y

Cuts at:      0     268     460    1110 
   Size:        268     192     650

  Fragments arranged by size: 

                 650     268     192
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     715     747    1063    1095    1110 
   Size:        715      32     316      32      15

  Fragments arranged by size: 

                 715     316      32      32      15
 
PpuMI rG'GwC_Cy

Cuts at:      0     219    1110 
   Size:        219     891
 
PstI C_TGCA'G

Cuts at:      0     662    1110 
   Size:        662     448
 
PvuII CAG'CTG

Cuts at:      0     357     501     942    1110 
   Size:        357     144     441     168

  Fragments arranged by size: 

                 441     357     168     144
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40    1110 
   Size:         40    1070
 
ScaI AGT'ACT

Cuts at:      0     664    1110 
   Size:        664     446
 
SexAI A'CCwGG_T

Cuts at:      0     221     593    1110 
   Size:        221     372     517

  Fragments arranged by size: 

                 517     372     221
 
SfcI C'TryA_G

Cuts at:      0     176     658    1110 
   Size:        176     482     452

  Fragments arranged by size: 

                 482     452     176
 
SmlI C'TyrA_G

Cuts at:      0    1099    1110 
   Size:       1099      11
 
SphI G_CATG'C

Cuts at:      0     268    1110 
   Size:        268     842
 
StuI AGG'CCT

Cuts at:      0     910    1110 
   Size:        910     200
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1017    1110 
   Size:       1017      93
 
TatI w'GTAC_w

Cuts at:      0     281     662    1110 
   Size:        281     381     448

  Fragments arranged by size: 

                 448     381     281
 
Tth111I GACn'n_nGTC

Cuts at:      0     223    1110 
   Size:        223     887
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     437    1110 
   Size:        437     673
 
XmnI GAAnn'nnTTC

Cuts at:      0     484     730    1110 
   Size:        484     246     380

  Fragments arranged by size: 

                 484     380     246
 

 Enzymes that do cut:

     AcuI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI     AvaI
     BbsI    BciVI     BglI Bme1580I     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrBI
   BstAPI   BstEII    BstXI   Bsu36I     BtgI     EaeI     EarI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I     MscI     MslI   MspA1I     NspI
     PpiI    PpuMI     PstI    PvuII     SapI     ScaI    SexAI     SfcI
     SmlI     SphI     StuI    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AlfI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI     TstI
     XbaI     XhoI     XmaI     ZraI
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