Restriction map for pF1KSDA0049
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflIII      AgeI        Alw44I      AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BbeI        BbrPI       
BcnI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstUI       Bsu36I      CelII       
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco52I      Eco81I      EcoO65I     EcoT22I     
FauI        FspI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NarI        NciI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        SspI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         1362  GTATAC               GT^[AC][GT]AC
AcyI         1376  GGCGTC               G[GA]^CG[CT]C
AflII         202  CTTAAG               C^TTAAG
AhdI         1675  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       198  AGCGCT               AGC^GCT
AspEI        1675  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1409  CTCGGG               C^[CT]CG[GA]G
BalI          744  TGGCCA               TGG^CCA
BamHI         387  GGATCC               G^GATCC
BcgI          394  GCAGTGCAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         166  GTATCC               GTATCC(6/5)
BfaI          304  CTAG                 C^TAG
BfrI          202  CTTAAG               C^TTAAG
BglI         2755  GCCCTGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1376  GGCGTC               G[GA]^CG[CT]C
BsaI          780  GAGACC               GGTCTC(1/5)
BsmBI         475  GAGACG               CGTCTC(1/5)
BsoBI        1409  CTCGGG               C^[CT]CG[GA]G
BsrFI        1306  GCCGGC               [GA]^CCGG[CT]
Bst1107I     1362  GTATAC               GTA^TAC
BstXI        1395  CCAGCAATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1362  GTATAC               GTA^TAC
Cfr10I       1306  GCCGGC               [GA]^CCGG[CT]
DsaI         1005  CCATGG               C^C[GA][CT]GG
Eam1105I     1675  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      198  AGCGCT               AGC^GCT
EcoNI         144  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         996  GAATTC               G^AATTC
EcoRV          88  GATATC               GAT^ATC
FseI         1305  GGCCGGCC             GGCCGG^CC
HaeII         198  AGCGCT               [GA]GCGC^[CT]
HapII        1307  CCGG                 C^CGG
HgaI         1377  GCGTC                GACGC(5/10)
Hin1I        1376  GGCGTC               G[GA]^CG[CT]C
HincII        496  GTTAAC               GT[CT]^[GA]AC
HindII        496  GTTAAC               GT[CT]^[GA]AC
HpaI          496  GTTAAC               GTT^AAC
HpaII        1307  CCGG                 C^CGG
MaeI          304  CTAG                 C^TAG
MluNI         744  TGGCCA               TGG^CCA
MscI          744  TGGCCA               TGG^CCA
MspI         1307  CCGG                 C^CGG
NaeI         1306  GCCGGC               GCC^GGC
NcoI         1005  CCATGG               C^CATGG
NdeI          740  CATATG               CA^TATG
NgoMIV       1306  GCCGGC               G^CCGGC
NspI         1763  GCATGT               [GA]CATG^[CT]
PshAI        2009  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         2601  GAAGAGC              GCTCTTC(1/4)
SbfI         2838  CCTGCAGG             CCTGCA^GG
ScaI          821  AGTACT               AGT^ACT
Sse8387I     2838  CCTGCAGG             CCTGCA^GG
StuI         2274  AGGCCT               AGG^CCT
TaqI           92  TCGA                 T^CGA
TthHB8I        92  TCGA                 T^CGA
XcmI         2225  CCAGCCGCCCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Asp700I      1525  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2854  GAACTTCTTC          
BanI          254  GGCACC               G^G[CT][GA]CC
             2621  GGCACC              
BbvCI        2086  GCTGAGG              CCTCAGC(-5/-2)
             2329  GCTGAGG             
BclI         1481  TGATCA               T^GATCA
             2547  TGATCA              
Bsp1407I     2249  TGTACA               T^GTACA
             2481  TGTACA              
BsrGI        2249  TGTACA               T^GTACA
             2481  TGTACA              
BstAPI        650  GCAACAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              906  GCAAAGGTTGC         
CfoI          199  GCGC                 GCG^C
             2260  GCGC                
DraI           31  TTTAAA               TTT^AAA
             1846  TTTAAA              
EaeI          744  TGGCCA               [CT]^GGCC[GA]
             1308  CGGCCA              
FbaI         1481  TGATCA               T^GATCA
             2547  TGATCA              
HhaI          199  GCGC                 GCG^C
             2260  GCGC                
HinP1I        199  GCGC                 G^CGC
             2260  GCGC                
PpuMI        1673  AGGACCT              [GA]G^G[AT]CC[CT]
             1933  GGGACCC             
SspBI        2249  TGTACA               T^GTACA
             2481  TGTACA              
XmnI         1525  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2854  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanII       
BbsI        BbvI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HindIII     HinfI       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       
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