Restriction map for pF1KSDA0049
 (Circular) (Six-base) MAPSORT of: pF1KSDA0049.seq  Check: 2977  from: 1  to: 6035



pF1KSDA0049  6035 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1645    1645 
   Size:       6035
 
AccI GT'mk_AC

Cuts at:   1489    3073    1489 
   Size:       1584    4451
 
Acc65I G'GTAC_C

Cuts at:   3051    3051 
   Size:       6035
 
AclI AA'CG_TT

Cuts at:   5875    5875 
   Size:       6035
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    707     916    1267    2189    2438    2648    2885    2962    2971 
   Size:        209     351     922     249     210     237      77       9

Cuts at:   2971    3860    4292    5039    5307     707 
   Size:        889     432     747     268    1435

  Fragments arranged by size: 

                1435     922     889     747     432     351     268     249
                 237     210     209      77       9
 
AfeI AGC'GCT

Cuts at:    326     326 
   Size:       6035
 
AflII C'TTAA_G

Cuts at:    328     328 
   Size:       6035
 
AflIII A'CryG_T

Cuts at:   3387    4491    5468    3387 
   Size:       1104     977    3954

  Fragments arranged by size: 

                3954    1104     977
 
AgeI A'CCGG_T

Cuts at:   4423    4423 
   Size:       6035
 
AhdI GACnn_n'nnGTC

Cuts at:   1806    1806 
   Size:       6035
 
AleI CACnn'nnGTG

Cuts at:   1870    1870 
   Size:       6035
 
AlwNI CAG_nnn'CTG

Cuts at:   1803    2538    2671    2845    3340    4907    1803 
   Size:        735     133     174     495    1567    2931

  Fragments arranged by size: 

                2931    1567     735     495     174     133
 
ApaLI G'TGCA_C

Cuts at:   4805    4805 
   Size:       6035
 
ApoI r'AATT_y

Cuts at:    167    1109    1122    1260    3039    5388    5644     167 
   Size:        942      13     138    1779    2349     256     558

  Fragments arranged by size: 

                2349    1779     942     558     256     138      13
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6035
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6035
 
AvaI C'yCGr_G

Cuts at:   1535    3055    1535 
   Size:       1520    4515
 
BamHI G'GATC_C

Cuts at:    513    3060     513 
   Size:       2547    3488
 
BanI G'GyrC_C

Cuts at:    380    2747    3051    3465    3713    3748     380 
   Size:       2367     304     414     248      35    2667

  Fragments arranged by size: 

                2667    2367     414     304     248      35
 
BanII G_rGCy'C

Cuts at:    922    1147    1585    1766    2276    2599    2693    3049    4079 
   Size:        225     438     181     510     323      94     356    1030

Cuts at:   4079     922 
   Size:       2878

  Fragments arranged by size: 

                2878    1030     510     438     356     323     225     181
                  94
 
BbeI G_GCGC'C

Cuts at:   3469    3717    3469 
   Size:        248    5787
 
BbsI GAAGACnn'nnnn_

Cuts at:    508     955    2054     508 
   Size:        447    1099    4489

  Fragments arranged by size: 

                4489    1099     447
 
BbvCI CC'TCA_GC

Cuts at:   2213    2456    2213 
   Size:        243    5792
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    509     543     509 
   Size:         34    6001
 
BciVI GTATCCnnnnn_n'

Cuts at:    303    3933    4694    6035     303 
   Size:       3630     761    1341     303

  Fragments arranged by size: 

                3630    1341     761     303
 
BclI T'GATC_A

Cuts at:   1607    2673    3555    1607 
   Size:       1066     882    4087

  Fragments arranged by size: 

                4087    1066     882
 
BglI GCCn_nnn'nGGC

Cuts at:   2887    2887 
   Size:       6035
 
BglII A'GATC_T

Cuts at:   3550    5587    3550 
   Size:       2037    3998
 
BlpI GC'TnA_GC

Cuts at:   3147    3147 
   Size:       6035
 
Bme1580I G_kGCm'C

Cuts at:    383    1859    2552    3660    3753    4809     383 
   Size:       1476     693    1108      93    1056    1609

  Fragments arranged by size: 

                1609    1476    1108    1056     693      93
 
BmgBI CAC'GTC

Cuts at:    888     888 
   Size:       6035
 
BmrI ACTGGGnnnn_n'

Cuts at:    403    2058    3247    3328    3663    5807     403 
   Size:       1655    1189      81     335    2144     631

  Fragments arranged by size: 

                2144    1655    1189     631     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4402    4402 
   Size:       6035
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    619     658     976    1123    1165    1332    2425    2498    2792 
   Size:         39     318     147      42     167    1093      73     294

Cuts at:   2792     619 
   Size:       3862

  Fragments arranged by size: 

                3862    1093     318     294     167     147      73      42
                  39
 
Bpu10I CC'TnA_GC

Cuts at:   2213    2456    2213 
   Size:        243    5792
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    548    1345    1411    1599    2727    3211    4090    4582    4880 
   Size:        797      66     188    1128     484     879     492     298

Cuts at:   4880     548 
   Size:       1703

  Fragments arranged by size: 

                1703    1128     879     797     492     484     298     188
                  66
 
BsaI GGTCTCn'nnnn_

Cuts at:    900    5739     900 
   Size:       4839    1196
 
BsaAI yAC'GTr

Cuts at:   3027    3388    3027 
   Size:        361    5674
 
BsaBI GATnn'nnATC

Cuts at:      6    3549    5586       6 
   Size:       3543    2037     455

  Fragments arranged by size: 

                3543    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1503    3466    3714    4416    5912    1503 
   Size:       1963     248     702    1496    1626

  Fragments arranged by size: 

                1963    1626    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   3224    3745    4423    4697    4844    5255    3224 
   Size:        521     678     274     147     411    4004

  Fragments arranged by size: 

                4004     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1471    1501    2765    2795    1471 
   Size:         30    1264      30    4711

  Fragments arranged by size: 

                4711    1264      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    912     947    1886    2209    2248    2251    2254    2263    2272 
   Size:         35     939     323      39       3       3       9       9

Cuts at:   2272    2275    2278    2699     912 
   Size:          3       3     421    4248

  Fragments arranged by size: 

                4248     939     421     323      39      35       9       9
                   3       3       3       3
 
BseYI C'CCAG_C

Cuts at:   1470    2440    2461    2830    3460    4218    4795    5291    1470 
   Size:        970      21     369     630     758     577     496    2214

  Fragments arranged by size: 

                2214     970     758     630     577     496     369      21
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    544    1262    1367    1566    2831     544 
   Size:        718     105     199    1265    3748

  Fragments arranged by size: 

                3748    1265     718     199     105
 
BsiEI CG_ry'CG

Cuts at:    104    3577    3623    4831     104 
   Size:       3473      46    1208    1308

  Fragments arranged by size: 

                3473    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1766    2276    2504    2599    3049    3827    4017    4809    1766 
   Size:        510     228      95     450     778     190     792    2992

  Fragments arranged by size: 

                2992     792     778     510     450     228     190      95
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:    595     595 
   Size:       6035
 
Bsp1286I G_dGCh'C

Cuts at:    383     922    1147    1585    1766    1859    2276    2504    2552 
   Size:        539     225     438     181      93     417     228      48

Cuts at:   2552    2599    2693    3049    3660    3753    3827    4017    4079 
   Size:         47      94     356     611      93      74     190      62

Cuts at:   4079    4809     383 
   Size:        730    1609

  Fragments arranged by size: 

                1609     730     611     539     438     417     356     228
                 225     190     181      94      93      93      74      62
                  48      47
 
BspEI T'CCGG_A

Cuts at:   5255    5255 
   Size:       6035
 
BspHI T'CATG_A

Cuts at:   5211    5211 
   Size:       6035
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1645    1811    2958    3085    3299    3601    3982    1645 
   Size:        166    1147     127     214     302     381    3698

  Fragments arranged by size: 

                3698    1147     381     302     214     166     127
 
BsrBI CCG'CTC

Cuts at:     50    4327    4381    5865    6030      50 
   Size:       4277      54    1484     165      55

  Fragments arranged by size: 

                4277    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1463    2021    2765    3947    1463 
   Size:        558     744    1182    3551

  Fragments arranged by size: 

                3551    1182     744     558
 
BsrFI r'CCGG_y

Cuts at:   1432    4033    4214    4423    1432 
   Size:       2601     181     209    3044

  Fragments arranged by size: 

                3044    2601     209     181
 
BsrGI T'GTAC_A

Cuts at:   2375    2607    2375 
   Size:        232    5803
 
BssSI C'ACGA_G

Cuts at:   4306    4664    4306 
   Size:        358    5677
 
BstAPI GCAn_nnn'nTGC

Cuts at:    782    1038    3412     782 
   Size:        256    2374    3405

  Fragments arranged by size: 

                3405    2374     256
 
BstBI TT'CG_AA

Cuts at:    110    4396     110 
   Size:       4286    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1528    1528 
   Size:       6035
 
BstYI r'GATC_y

Cuts at:      1     513    1560    1782    3060    3527    3550    3885    4131 
   Size:        512    1047     222    1278     467      23     335     246

Cuts at:   4131    5143    5229    5241    5587       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1278    1047    1012     512     467     449     346     335
                 246     222      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   1490    1490 
   Size:       6035
 
BtgI C'CryG_G

Cuts at:    125    1131     125 
   Size:       1006    5029
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3985    4166    4329    3985 
   Size:        181     163    5691

  Fragments arranged by size: 

                5691     181     163
 
BtsI GCAGTG_nn'

Cuts at:    527    2584    2843    3278     527 
   Size:       2057     259     435    3284

  Fragments arranged by size: 

                3284    2057     435     259
 
DraI TTT'AAA

Cuts at:    159    1974    3034     159 
   Size:       1815    1060    3160

  Fragments arranged by size: 

                3160    1815    1060
 
DrdI GACnn_nn'nnGTC

Cuts at:   3741    4599    3741 
   Size:        858    5177
 
EaeI y'GGCC_r

Cuts at:    870    1434    3620    3794    4185    4212    5557     870 
   Size:        564    2186     174     391      27    1345    1348

  Fragments arranged by size: 

                2186    1348    1345     564     391     174      27
 
EagI C'GGCC_G

Cuts at:   3620    3620 
   Size:       6035
 
EarI CTCTTCn'nnn_

Cuts at:    281     474    1571    1946    2722    4058    4268     281 
   Size:        193    1097     375     776    1336     210    2048

  Fragments arranged by size: 

                2048    1336    1097     776     375     210     193
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4243    4553    4699    5845    4243 
   Size:        310     146    1146    4433

  Fragments arranged by size: 

                4433    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1764    2274    2597    3047    1764 
   Size:        510     323     450    4752

  Fragments arranged by size: 

                4752     510     450     323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    619     658     707     916     976    1123    1165    1267    1332 
   Size:         39      49     209      60     147      42     102      65

Cuts at:   1332    2189    2425    2438    2498    2648    2792    2885    2962 
   Size:        857     236      13      60     150     144      93      77

Cuts at:   2962    2971    3860    4292    5039    5307     619 
   Size:          9     889     432     747     268    1347

  Fragments arranged by size: 

                1347     889     857     747     432     268     236     209
                 150     147     144     102      93      77      65      60
                  60      49      42      39      13       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:    274     274 
   Size:       6035
 
EcoO109I rG'GnC_Cy

Cuts at:   1800    2060    2820    3174    1800 
   Size:        260     760     354    4661

  Fragments arranged by size: 

                4661     760     354     260
 
EcoRI G'AATT_C

Cuts at:   1122    3039    1122 
   Size:       1917    4118
 
EcoRV GAT'ATC

Cuts at:    216     216 
   Size:       6035
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    343    1950    2194    4054     343 
   Size:       1607     244    1860    2324

  Fragments arranged by size: 

                2324    1860    1607     244
 
FseI GG_CCGG'CC

Cuts at:   1436    1436 
   Size:       6035
 
FspI TGC'GCA

Cuts at:   3816    3816 
   Size:       6035
 
HaeII r_GCGC'y

Cuts at:    328    3469    3717    4739    5718     328 
   Size:       3141     248    1022     979     645

  Fragments arranged by size: 

                3141    1022     979     645     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1792    1824    2877    2909    3877    3907    3909    3939    1792 
   Size:         32    1053      32     968      30       2      30    3888

  Fragments arranged by size: 

                3888    1053     968      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    624    3074     624 
   Size:       2450    3585
 
HindIII A'AGCT_T

Cuts at:    174     640     664     174 
   Size:        466      24    5545

  Fragments arranged by size: 

                5545     466      24
 
HpaI GTT'AAC

Cuts at:    624     624 
   Size:       6035
 
KasI G'GCGC_C

Cuts at:   3465    3713    3465 
   Size:        248    5787
 
KpnI G_GTAC'C

Cuts at:   3055    3055 
   Size:       6035
 
MluI A'CGCG_T

Cuts at:   5468    5468 
   Size:       6035
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1802    1826    4706    4890    1802 
   Size:         24    2880     184    2947

  Fragments arranged by size: 

                2947    2880     184      24
 
MscI TGG'CCA

Cuts at:    872    3796     872 
   Size:       2924    3111
 
MslI CAynn'nnrTG

Cuts at:   1870    2615    2753    4024    4151    1870 
   Size:        745     138    1271     127    3754

  Fragments arranged by size: 

                3754    1271     745     138     127
 
MspA1I CmG'CkG

Cuts at:    761    2455    2925    3146    3460    3820    4833    5078     761 
   Size:       1694     470     221     314     360    1013     245    1718

  Fragments arranged by size: 

                1718    1694    1013     470     360     314     245     221
            
 
NaeI GCC'GGC

Cuts at:   1434    4216    1434 
   Size:       2782    3253
 
NarI GG'CG_CC

Cuts at:   3466    3714    3466 
   Size:        248    5787
 
NcoI C'CATG_G

Cuts at:    125    1131     125 
   Size:       1006    5029
 
NdeI CA'TA_TG

Cuts at:    867     867 
   Size:       6035
 
NgoMIV G'CCGG_C

Cuts at:   1432    4214    1432 
   Size:       2782    3253
 
NspI r_CATG'y

Cuts at:   1893    3088    3588    4495    1893 
   Size:       1195     500     907    3433

  Fragments arranged by size: 

                3433    1195     907     500
 
PasI CC'CwG_GG

Cuts at:   2361    2361 
   Size:       6035
 
PciI A'CATG_T

Cuts at:   4491    4491 
   Size:       6035
 
PmeI GTTT'AAAC

Cuts at:   3034    3034 
   Size:       6035
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5174    5206    5174 
   Size:         32    6003
 
PpuMI rG'GwC_Cy

Cuts at:   1800    2060    1800 
   Size:        260    5775
 
PshAI GACnn'nnGTC

Cuts at:   2139    2139 
   Size:       6035
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    638     670     638 
   Size:         32    6003
 
PstI C_TGCA'G

Cuts at:    362     522    2969    3082    3767     362 
   Size:        160    2447     113     685    2630

  Fragments arranged by size: 

                2630    2447     685     160     113
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6035
 
PvuII CAG'CTG

Cuts at:    761    2455    2925    3460    3820     761 
   Size:       1694     470     535     360    2976

  Fragments arranged by size: 

                2976    1694     535     470     360
 
RsrII CG'GwC_CG

Cuts at:   4230    4230 
   Size:       6035
 
SacI G_AGCT'C

Cuts at:   1766    2276    2599    3049    1766 
   Size:        510     323     450    4752

  Fragments arranged by size: 

                4752     510     450     323
 
SalI G'TCGA_C

Cuts at:   3072    3072 
   Size:       6035
 
SanDI GG'GwC_CC

Cuts at:   2060    2060 
   Size:       6035
 
SapI GCTCTTCn'nnn_

Cuts at:   2722    4058    4268    2722 
   Size:       1336     210    4489

  Fragments arranged by size: 

                4489    1336     210
 
SbfI CC_TGCA'GG

Cuts at:   2969    3082    2969 
   Size:        113    5922
 
ScaI AGT'ACT

Cuts at:    949    5552     949 
   Size:       4603    1432
 
SfcI C'TryA_G

Cuts at:     33     358     518    2965    3078    3763    4756    4947      33 
   Size:        325     160    2447     113     685     993     191    1121

  Fragments arranged by size: 

                2447    1121     993     685     325     191     160     113
            
 
SfoI GGC'GCC

Cuts at:   3467    3715    3467 
   Size:        248    5787
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6035
 
SmaI CCC'GGG

Cuts at:   3057    3057 
   Size:       6035
 
SmlI C'TyrA_G

Cuts at:    328     563    1360    1426    1614    2742    3190    4105    4597 
   Size:        235     797      66     188    1128     448     915     492

Cuts at:   4597    4859     328 
   Size:        262    1504

  Fragments arranged by size: 

                1504    1128     915     797     492     448     262     235
                 188      66
 
SnaBI TAC'GTA

Cuts at:   3027    3027 
   Size:       6035
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6035
 
SphI G_CATG'C

Cuts at:   3088    3588    3088 
   Size:        500    5535
 
StuI AGG'CCT

Cuts at:   2402    2402 
   Size:       6035
 
StyI C'CwwG_G

Cuts at:    125     686    1131    1475    2099    3169    3523     125 
   Size:        561     445     344     624    1070     354    2637

  Fragments arranged by size: 

                2637    1070     624     561     445     354     344
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3618    3751    4210    4419    3618 
   Size:        133     459     209    5234

  Fragments arranged by size: 

                5234     459     209     133
 
TatI w'GTAC_w

Cuts at:    947    2375    2607    5550     947 
   Size:       1428     232    2943    1432

  Fragments arranged by size: 

                2943    1432    1428     232
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    786     818    2765    2797    5552    5584     786 
   Size:         32    1947      32    2755      32    1237

  Fragments arranged by size: 

                2755    1947    1237      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3832    3832 
   Size:       6035
 
XbaI T'CTAG_A

Cuts at:   3066    3066 
   Size:       6035
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2358    3530    2358 
   Size:       1172    4863
 
XmaI C'CCGG_G

Cuts at:   3055    3055 
   Size:       6035
 
XmnI GAAnn'nnTTC

Cuts at:   1655    2984    1655 
   Size:       1329    4706
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NspI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AlfI     AloI     ApaI     AscI    AvrII     BaeI    BfrBI
     BmtI    BsiWI     BsmI   BssHII   BstEII   Bsu36I     ClaI    CspCI
   DraIII    FspAI     MfeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmlI     PsiI   PspOMI    PspXI    SacII    SexAI
     SfiI    SgrAI     SrfI     SspI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com