Restriction map for pF1KSDA0049
 (Linear) (Six-base) MAPSORT of: KIAA0049.seq  Check: 6537  from: 1  to: 2898



KIAA0049.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1519    2898 
   Size:       1519    1379
 
AccI GT'mk_AC

Cuts at:      0    1363    2898 
   Size:       1363    1535
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     790    1141    2063    2312    2522    2759    2836 
   Size:        581     209     351     922     249     210     237      77

Cuts at:   2836    2845    2898 
   Size:          9      53

  Fragments arranged by size: 

                 922     581     351     249     237     210     209      77
                  53       9
 
AfeI AGC'GCT

Cuts at:      0     200    2898 
   Size:        200    2698
 
AflII C'TTAA_G

Cuts at:      0     202    2898 
   Size:        202    2696
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1680    2898 
   Size:       1680    1218
 
AleI CACnn'nnGTG

Cuts at:      0    1744    2898 
   Size:       1744    1154
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1677    2412    2545    2719    2898 
   Size:       1677     735     133     174     179

  Fragments arranged by size: 

                1677     735     179     174     133
 
ApoI r'AATT_y

Cuts at:      0      41     983     996    1134    2898 
   Size:         41     942      13     138    1764

  Fragments arranged by size: 

                1764     942     138      41      13
 
AvaI C'yCGr_G

Cuts at:      0    1409    2898 
   Size:       1409    1489
 
BamHI G'GATC_C

Cuts at:      0     387    2898 
   Size:        387    2511
 
BanI G'GyrC_C

Cuts at:      0     254    2621    2898 
   Size:        254    2367     277

  Fragments arranged by size: 

                2367     277     254
 
BanII G_rGCy'C

Cuts at:      0     796    1021    1459    1640    2150    2473    2567    2898 
   Size:        796     225     438     181     510     323      94     331

  Fragments arranged by size: 

                 796     510     438     331     323     225     181      94
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     382     829    1928    2898 
   Size:        382     447    1099     970

  Fragments arranged by size: 

                1099     970     447     382
 
BbvCI CC'TCA_GC

Cuts at:      0    2087    2330    2898 
   Size:       2087     243     568

  Fragments arranged by size: 

                2087     568     243
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     383     417    2898 
   Size:        383      34    2481

  Fragments arranged by size: 

                2481     383      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     177    2898 
   Size:        177    2721
 
BclI T'GATC_A

Cuts at:      0    1481    2547    2898 
   Size:       1481    1066     351

  Fragments arranged by size: 

                1481    1066     351
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2761    2898 
   Size:       2761     137
 
Bme1580I G_kGCm'C

Cuts at:      0     257    1733    2426    2898 
   Size:        257    1476     693     472

  Fragments arranged by size: 

                1476     693     472     257
 
BmgBI CAC'GTC

Cuts at:      0     762    2898 
   Size:        762    2136
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277    1932    2898 
   Size:        277    1655     966

  Fragments arranged by size: 

                1655     966     277
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     532     850     997    1039    1206    2299    2372 
   Size:        493      39     318     147      42     167    1093      73

Cuts at:   2372    2666    2898 
   Size:        294     232

  Fragments arranged by size: 

                1093     493     318     294     232     167     147      73
                  42      39
 
Bpu10I CC'TnA_GC

Cuts at:      0    2087    2330    2898 
   Size:       2087     243     568

  Fragments arranged by size: 

                2087     568     243
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     422    1219    1285    1473    2601    2898 
   Size:        422     797      66     188    1128     297

  Fragments arranged by size: 

                1128     797     422     297     188      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     774    2898 
   Size:        774    2124
 
BsaHI Gr'CG_yC

Cuts at:      0    1377    2898 
   Size:       1377    1521
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1345    1375    2639    2669    2898 
   Size:       1345      30    1264      30     229

  Fragments arranged by size: 

                1345    1264     229      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     786     821    1760    2083    2122    2125    2128    2137 
   Size:        786      35     939     323      39       3       3       9

Cuts at:   2137    2146    2149    2152    2573    2898 
   Size:          9       3       3     421     325

  Fragments arranged by size: 

                 939     786     421     325     323      39      35       9
                   9       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1344    2314    2335    2704    2898 
   Size:       1344     970      21     369     194

  Fragments arranged by size: 

                1344     970     369     194      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1136    1241    1440    2705    2898 
   Size:        418     718     105     199    1265     193

  Fragments arranged by size: 

                1265     718     418     199     193     105
 
BsiHKAI G_wGCw'C

Cuts at:      0    1640    2150    2378    2473    2898 
   Size:       1640     510     228      95     425

  Fragments arranged by size: 

                1640     510     425     228      95
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     469    2898 
   Size:        469    2429
 
Bsp1286I G_dGCh'C

Cuts at:      0     257     796    1021    1459    1640    1733    2150    2378 
   Size:        257     539     225     438     181      93     417     228

Cuts at:   2378    2426    2473    2567    2898 
   Size:         48      47      94     331

  Fragments arranged by size: 

                 539     438     417     331     257     228     225     181
                  94      93      48      47
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1519    1685    2832    2898 
   Size:       1519     166    1147      66

  Fragments arranged by size: 

                1519    1147     166      66
 
BsrDI GCAATG_nn'

Cuts at:      0    1337    1895    2639    2898 
   Size:       1337     558     744     259

  Fragments arranged by size: 

                1337     744     558     259
 
BsrFI r'CCGG_y

Cuts at:      0    1306    2898 
   Size:       1306    1592
 
BsrGI T'GTAC_A

Cuts at:      0    2249    2481    2898 
   Size:       2249     232     417

  Fragments arranged by size: 

                2249     417     232
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     656     912    2898 
   Size:        656     256    1986

  Fragments arranged by size: 

                1986     656     256
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1402    2898 
   Size:       1402    1496
 
BstYI r'GATC_y

Cuts at:      0     387    1434    1656    2898 
   Size:        387    1047     222    1242

  Fragments arranged by size: 

                1242    1047     387     222
 
BstZ17I GTA'TAC

Cuts at:      0    1364    2898 
   Size:       1364    1534
 
BtgI C'CryG_G

Cuts at:      0    1005    2898 
   Size:       1005    1893
 
BtsI GCAGTG_nn'

Cuts at:      0     401    2458    2717    2898 
   Size:        401    2057     259     181

  Fragments arranged by size: 

                2057     401     259     181
 
DraI TTT'AAA

Cuts at:      0      33    1848    2898 
   Size:         33    1815    1050

  Fragments arranged by size: 

                1815    1050      33
 
EaeI y'GGCC_r

Cuts at:      0     744    1308    2898 
   Size:        744     564    1590

  Fragments arranged by size: 

                1590     744     564
 
EarI CTCTTCn'nnn_

Cuts at:      0     155     348    1445    1820    2596    2898 
   Size:        155     193    1097     375     776     302

  Fragments arranged by size: 

                1097     776     375     302     193     155
 
EcoICRI GAG'CTC

Cuts at:      0    1638    2148    2471    2898 
   Size:       1638     510     323     427

  Fragments arranged by size: 

                1638     510     427     323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     532     581     790     850     997    1039    1141 
   Size:        493      39      49     209      60     147      42     102

Cuts at:   1141    1206    2063    2299    2312    2372    2522    2666    2759 
   Size:         65     857     236      13      60     150     144      93

Cuts at:   2759    2836    2845    2898 
   Size:         77       9      53

  Fragments arranged by size: 

                 857     493     236     209     150     147     144     102
                  93      77      65      60      60      53      49      42
                  39      13       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     148    2898 
   Size:        148    2750
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1674    1934    2694    2898 
   Size:       1674     260     760     204

  Fragments arranged by size: 

                1674     760     260     204
 
EcoRI G'AATT_C

Cuts at:      0     996    2898 
   Size:        996    1902
 
EcoRV GAT'ATC

Cuts at:      0      90    2898 
   Size:         90    2808
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     217    1824    2068    2898 
   Size:        217    1607     244     830

  Fragments arranged by size: 

                1607     830     244     217
 
FseI GG_CCGG'CC

Cuts at:      0    1310    2898 
   Size:       1310    1588
 
HaeII r_GCGC'y

Cuts at:      0     202    2898 
   Size:        202    2696
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1666    1698    2751    2783    2898 
   Size:       1666      32    1053      32     115

  Fragments arranged by size: 

                1666    1053     115      32      32
 
HincII GTy'rAC

Cuts at:      0     498    2898 
   Size:        498    2400
 
HindIII A'AGCT_T

Cuts at:      0      48     514     538    2898 
   Size:         48     466      24    2360

  Fragments arranged by size: 

                2360     466      48      24
 
HpaI GTT'AAC

Cuts at:      0     498    2898 
   Size:        498    2400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1676    1700    2898 
   Size:       1676      24    1198

  Fragments arranged by size: 

                1676    1198      24
 
MscI TGG'CCA

Cuts at:      0     746    2898 
   Size:        746    2152
 
MslI CAynn'nnrTG

Cuts at:      0    1744    2489    2627    2898 
   Size:       1744     745     138     271

  Fragments arranged by size: 

                1744     745     271     138
 
MspA1I CmG'CkG

Cuts at:      0     635    2329    2799    2898 
   Size:        635    1694     470      99

  Fragments arranged by size: 

                1694     635     470      99
 
NaeI GCC'GGC

Cuts at:      0    1308    2898 
   Size:       1308    1590
 
NcoI C'CATG_G

Cuts at:      0    1005    2898 
   Size:       1005    1893
 
NdeI CA'TA_TG

Cuts at:      0     741    2898 
   Size:        741    2157
 
NgoMIV G'CCGG_C

Cuts at:      0    1306    2898 
   Size:       1306    1592
 
NspI r_CATG'y

Cuts at:      0    1767    2898 
   Size:       1767    1131
 
PasI CC'CwG_GG

Cuts at:      0    2235    2898 
   Size:       2235     663
 
PpuMI rG'GwC_Cy

Cuts at:      0    1674    1934    2898 
   Size:       1674     260     964

  Fragments arranged by size: 

                1674     964     260
 
PshAI GACnn'nnGTC

Cuts at:      0    2013    2898 
   Size:       2013     885
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     512     544    2898 
   Size:        512      32    2354

  Fragments arranged by size: 

                2354     512      32
 
PstI C_TGCA'G

Cuts at:      0     236     396    2843    2898 
   Size:        236     160    2447      55

  Fragments arranged by size: 

                2447     236     160      55
 
PvuII CAG'CTG

Cuts at:      0     635    2329    2799    2898 
   Size:        635    1694     470      99

  Fragments arranged by size: 

                1694     635     470      99
 
SacI G_AGCT'C

Cuts at:      0    1640    2150    2473    2898 
   Size:       1640     510     323     425

  Fragments arranged by size: 

                1640     510     425     323
 
SanDI GG'GwC_CC

Cuts at:      0    1934    2898 
   Size:       1934     964
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2596    2898 
   Size:       2596     302
 
SbfI CC_TGCA'GG

Cuts at:      0    2843    2898 
   Size:       2843      55
 
ScaI AGT'ACT

Cuts at:      0     823    2898 
   Size:        823    2075
 
SfcI C'TryA_G

Cuts at:      0     232     392    2839    2898 
   Size:        232     160    2447      59

  Fragments arranged by size: 

                2447     232     160      59
 
SmlI C'TyrA_G

Cuts at:      0     202     437    1234    1300    1488    2616    2898 
   Size:        202     235     797      66     188    1128     282

  Fragments arranged by size: 

                1128     797     282     235     202     188      66
 
StuI AGG'CCT

Cuts at:      0    2276    2898 
   Size:       2276     622
 
StyI C'CwwG_G

Cuts at:      0     560    1005    1349    1973    2898 
   Size:        560     445     344     624     925

  Fragments arranged by size: 

                 925     624     560     445     344
 
TatI w'GTAC_w

Cuts at:      0     821    2249    2481    2898 
   Size:        821    1428     232     417

  Fragments arranged by size: 

                1428     821     417     232
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     660     692    2639    2671    2898 
   Size:        660      32    1947      32     227

  Fragments arranged by size: 

                1947     660     227      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2232    2898 
   Size:       2232     666
 
XmnI GAAnn'nnTTC

Cuts at:      0    1529    2858    2898 
   Size:       1529    1329      40

  Fragments arranged by size: 

                1529    1329      40
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII     AhdI     AleI    AlwNI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI
    BstYI  BstZ17I     BtgI     BtsI     DraI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    HaeII
    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NspI     PasI    PpuMI    PshAI
     PsrI     PstI    PvuII     SacI    SanDI     SapI     SbfI     ScaI
     SfcI     SmlI     StuI     StyI     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI   AflIII     AgeI     AlfI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BfrBI
    BglII     BlpI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BsrBI   BssHII    BssSI    BstBI
   BstEII   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EciI     FspI    FspAI     KasI     KpnI     MfeI     MluI     NarI
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PvuI    RsrII
    SacII     SalI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     ZraI
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