Restriction map for pF1KSDA0048
 (Circular) (Six-base) MAPSORT of: pF1KSDA0048.seq  Check: 7758  from: 1  to: 4955



pF1KSDA0048  4955 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    885    1029     885 
   Size:        144    4811
 
AatII G_ACGT'C

Cuts at:   1723    1723 
   Size:       4955
 
AccI GT'mk_AC

Cuts at:   1166    1993    1166 
   Size:        827    4128
 
Acc65I G'GTAC_C

Cuts at:   1971    1971 
   Size:       4955
 
AclI AA'CG_TT

Cuts at:   4795    4795 
   Size:       4955
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    370    2780    3212    3959    4227     370 
   Size:       2410     432     747     268    1098

  Fragments arranged by size: 

                2410    1098     747     432     268
 
AfeI AGC'GCT

Cuts at:   1882    1882 
   Size:       4955
 
AflIII A'CryG_T

Cuts at:    226    1621    2307    3411    4388     226 
   Size:       1395     686    1104     977     793

  Fragments arranged by size: 

                1395    1104     977     793     686
 
AgeI A'CCGG_T

Cuts at:   3343    3343 
   Size:       4955
 
AleI CACnn'nnGTG

Cuts at:    682     682 
   Size:       4955
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1328    1328 
   Size:       4955
 
AlwNI CAG_nnn'CTG

Cuts at:   1065    1488    2260    3827    1065 
   Size:        423     772    1567    2193

  Fragments arranged by size: 

                2193    1567     772     423
 
ApaI G_GGCC'C

Cuts at:    786     786 
   Size:       4955
 
ApaLI G'TGCA_C

Cuts at:   3725    3725 
   Size:       4955
 
ApoI r'AATT_y

Cuts at:   1959    4308    4564    1959 
   Size:       2349     256    2350

  Fragments arranged by size: 

                2350    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4955
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4955
 
AvaI C'yCGr_G

Cuts at:   1591    1975    1591 
   Size:        384    4571
 
BamHI G'GATC_C

Cuts at:   1980    1980 
   Size:       4955
 
BanI G'GyrC_C

Cuts at:    586    1014    1604    1971    2385    2633    2668     586 
   Size:        428     590     367     414     248      35    2873

  Fragments arranged by size: 

                2873     590     428     414     367     248      35
 
BanII G_rGCy'C

Cuts at:    786     973    1540    1820    1969    2999     786 
   Size:        187     567     280     149    1030    2742

  Fragments arranged by size: 

                2742    1030     567     280     187     149
 
BbeI G_GCGC'C

Cuts at:   1608    2389    2637    1608 
   Size:        781     248    3926

  Fragments arranged by size: 

                3926     781     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2853    3614    4955    2853 
   Size:        761    1341    2853

  Fragments arranged by size: 

                2853    1341     761
 
BclI T'GATC_A

Cuts at:    461    2475     461 
   Size:       2014    2941
 
BglI GCCn_nnn'nGGC

Cuts at:   1390    1390 
   Size:       4955
 
BglII A'GATC_T

Cuts at:   2470    4507    2470 
   Size:       2037    2918
 
BlpI GC'TnA_GC

Cuts at:    890    1145    1534    2067     890 
   Size:        255     389     533    3778

  Fragments arranged by size: 

                3778     533     389     255
 
Bme1580I G_kGCm'C

Cuts at:    786    1019    1737    2580    2673    3729     786 
   Size:        233     718     843      93    1056    2012

  Fragments arranged by size: 

                2012    1056     843     718     233      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    146    1380    1863    2167    2248    2583    4727     146 
   Size:       1234     483     304      81     335    2144     374

  Fragments arranged by size: 

                2144    1234     483     374     335     304      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3322    3322 
   Size:       4955
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    230     443    1676     230 
   Size:        213    1233    3509

  Fragments arranged by size: 

                3509    1233     213
 
Bpu10I CC'TnA_GC

Cuts at:    207     207 
   Size:       4955
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2131    3010    3502    3800    2131 
   Size:        879     492     298    3286

  Fragments arranged by size: 

                3286     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    910    1576    4659     910 
   Size:        666    3083    1206

  Fragments arranged by size: 

                3083    1206     666
 
BsaAI yAC'GTr

Cuts at:   1622    1947    2308    1622 
   Size:        325     361    4269

  Fragments arranged by size: 

                4269     361     325
 
BsaBI GATnn'nnATC

Cuts at:      6     574    2469    4506       6 
   Size:        568    1895    2037     455

  Fragments arranged by size: 

                2037    1895     568     455
 
BsaHI Gr'CG_yC

Cuts at:   1605    1720    2386    2634    3336    4832    1605 
   Size:        115     666     248     702    1496    1728

  Fragments arranged by size: 

                1728    1496     702     666     248     115
 
BsaWI w'CCGG_w

Cuts at:   1183    2144    2665    3343    3617    3764    4175    1183 
   Size:        961     521     678     274     147     411    1963

  Fragments arranged by size: 

                1963     961     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1636    1666    1636 
   Size:         30    4925
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    787    1905     787 
   Size:       1118    3837
 
BseYI C'CCAG_C

Cuts at:    194     869    1055    1221    1353    1586    2380    3138    3715 
   Size:        675     186     166     132     233     794     758     577

Cuts at:   3715    4211     194 
   Size:        496     938

  Fragments arranged by size: 

                 938     794     758     675     577     496     233     186
                 166     132
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    706    1096    1213    1516    1602    1732     706 
   Size:        390     117     303      86     130    3929

  Fragments arranged by size: 

                3929     390     303     130     117      86
 
BsiEI CG_ry'CG

Cuts at:    104    2497    2543    3751     104 
   Size:       2393      46    1208    1308

  Fragments arranged by size: 

                2393    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1278    1820    1969    2747    2937    3729    1278 
   Size:        542     149     778     190     792    2504

  Fragments arranged by size: 

                2504     792     778     542     190     149
 
BsmI GAATG_Cn'

Cuts at:   1383    1383 
   Size:       4955
 
BsmBI CGTCTCn'nnnn_

Cuts at:    895     895 
   Size:       4955
 
Bsp1286I G_dGCh'C

Cuts at:    786     973    1019    1278    1540    1737    1820    1969    2580 
   Size:        187      46     259     262     197      83     149     611

Cuts at:   2580    2673    2747    2937    2999    3729     786 
   Size:         93      74     190      62     730    2012

  Fragments arranged by size: 

                2012     730     611     262     259     197     190     187
                 149      93      83      74      62      46
 
BspEI T'CCGG_A

Cuts at:   4175    4175 
   Size:       4955
 
BspHI T'CATG_A

Cuts at:   1886    4131    1886 
   Size:       2245    2710
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    170     327     885     962     993    1029    2005    2219    2521 
   Size:        157     558      77      31      36     976     214     302

Cuts at:   2521    2902     170 
   Size:        381    2223

  Fragments arranged by size: 

                2223     976     558     381     302     214     157      77
                  36      31
 
BsrBI CCG'CTC

Cuts at:     50     692    1761    3247    3301    4785    4950      50 
   Size:        642    1069    1486      54    1484     165      55

  Fragments arranged by size: 

                1486    1484    1069     642     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    735    1415    2867     735 
   Size:        680    1452    2823

  Fragments arranged by size: 

                2823    1452     680
 
BsrFI r'CCGG_y

Cuts at:   2953    3134    3343    2953 
   Size:        181     209    4565

  Fragments arranged by size: 

                4565     209     181
 
BssSI C'ACGA_G

Cuts at:    760    3226    3584     760 
   Size:       2466     358    2131

  Fragments arranged by size: 

                2466    2131     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1065    1231    1273    1616    2332    1065 
   Size:        166      42     343     716    3688

  Fragments arranged by size: 

                3688     716     343     166      42
 
BstBI TT'CG_AA

Cuts at:    110    3316     110 
   Size:       3206    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1288    1288 
   Size:       4955
 
BstYI r'GATC_y

Cuts at:      1     569    1049    1133    1547    1980    2447    2470    2805 
   Size:        568     480      84     414     433     467      23     335

Cuts at:   2805    3051    4063    4149    4161    4507       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     568     480     467     449     433     414     346
                 335     246      86      84      23      12
 
BtgI C'CryG_G

Cuts at:    125     295     923     125 
   Size:        170     628    4157

  Fragments arranged by size: 

                4157     628     170
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2905    3086    3249    2905 
   Size:        181     163    4611

  Fragments arranged by size: 

                4611     181     163
 
BtsI GCAGTG_nn'

Cuts at:    134    2198     134 
   Size:       2064    2891
 
DraI TTT'AAA

Cuts at:   1954    1954 
   Size:       4955
 
DraIII CAC_nnn'GTG

Cuts at:   1810    1810 
   Size:       4955
 
DrdI GACnn_nn'nnGTC

Cuts at:   2661    3519    2661 
   Size:        858    4097
 
EaeI y'GGCC_r

Cuts at:   1574    2540    2714    3105    3132    4477    1574 
   Size:        966     174     391      27    1345    2052

  Fragments arranged by size: 

                2052    1345     966     391     174      27
 
EagI C'GGCC_G

Cuts at:   2540    2540 
   Size:       4955
 
EarI CTCTTCn'nnn_

Cuts at:    847    1642    1789    2978    3188     847 
   Size:        795     147    1189     210    2614

  Fragments arranged by size: 

                2614    1189     795     210     147
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3163    3473    3619    4765    3163 
   Size:        310     146    1146    3353

  Fragments arranged by size: 

                3353    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1818    1967    1818 
   Size:        149    4806
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    230     370     443    1676    2780    3212    3959    4227     230 
   Size:        140      73    1233    1104     432     747     268     958

  Fragments arranged by size: 

                1233    1104     958     747     432     268     140      73
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1207    1207 
   Size:       4955
 
EcoO109I rG'GnC_Cy

Cuts at:    473     782     783    2094     473 
   Size:        309       1    1311    3334

  Fragments arranged by size: 

                3334    1311     309       1
 
EcoRI G'AATT_C

Cuts at:   1959    1959 
   Size:       4955
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    369    1740    2974     369 
   Size:       1371    1234    2350

  Fragments arranged by size: 

                2350    1371    1234
 
FspI TGC'GCA

Cuts at:   2736    2736 
   Size:       4955
 
HaeII r_GCGC'y

Cuts at:    413    1608    1884    2389    2637    3659    4638     413 
   Size:       1195     276     505     248    1022     979     730

  Fragments arranged by size: 

                1195    1022     979     730     505     276     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    726     758    1143    1175    1636    1668    2797    2827    2829 
   Size:         32     385      32     461      32    1129      30       2

Cuts at:   2829    2859     726 
   Size:         30    2822

  Fragments arranged by size: 

                2822    1129     461     385      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    713     753    1994     713 
   Size:         40    1241    3674

  Fragments arranged by size: 

                3674    1241      40
 
KasI G'GCGC_C

Cuts at:   1604    2385    2633    1604 
   Size:        781     248    3926

  Fragments arranged by size: 

                3926     781     248
 
KpnI G_GTAC'C

Cuts at:   1975    1975 
   Size:       4955
 
MluI A'CGCG_T

Cuts at:   4388    4388 
   Size:       4955
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1845    3626    3810    1845 
   Size:       1781     184    2990

  Fragments arranged by size: 

                2990    1781     184
 
MscI TGG'CCA

Cuts at:   2716    2716 
   Size:       4955
 
MslI CAynn'nnrTG

Cuts at:    682    1286    2944    3071     682 
   Size:        604    1658     127    2566

  Fragments arranged by size: 

                2566    1658     604     127
 
MspA1I CmG'CkG

Cuts at:    297     432     925     991    1144    1231    1312    1399    1488 
   Size:        135     493      66     153      87      81      87      89

Cuts at:   1488    1554    2066    2380    2740    3753    3998     297 
   Size:         66     512     314     360    1013     245    1254

  Fragments arranged by size: 

                1254    1013     512     493     360     314     245     153
                 135      89      87      87      81      66      66
 
NaeI GCC'GGC

Cuts at:   3136    3136 
   Size:       4955
 
NarI GG'CG_CC

Cuts at:   1605    2386    2634    1605 
   Size:        781     248    3926

  Fragments arranged by size: 

                3926     781     248
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4955
 
NgoMIV G'CCGG_C

Cuts at:   3134    3134 
   Size:       4955
 
NspI r_CATG'y

Cuts at:    230    1831    2008    2508    3415     230 
   Size:       1601     177     500     907    1770

  Fragments arranged by size: 

                1770    1601     907     500     177
 
PciI A'CATG_T

Cuts at:    226    3411     226 
   Size:       3185    1770
 
PfoI T'CCnGG_A

Cuts at:    420    1208     420 
   Size:        788    4167
 
PmeI GTTT'AAAC

Cuts at:   1954    1954 
   Size:       4955
 
PmlI CAC'GTG

Cuts at:   1622    1622 
   Size:       4955
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4094    4126    4094 
   Size:         32    4923
 
PpuMI rG'GwC_Cy

Cuts at:    473     473 
   Size:       4955
 
PspOMI G'GGCC_C

Cuts at:    782     782 
   Size:       4955
 
PstI C_TGCA'G

Cuts at:    167    1236    2002    2687     167 
   Size:       1069     766     685    2435

  Fragments arranged by size: 

                2435    1069     766     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4955
 
PvuII CAG'CTG

Cuts at:    432     991    1144    1231    1312    1399    1488    1554    2380 
   Size:        559     153      87      81      87      89      66     826

Cuts at:   2380    2740     432 
   Size:        360    2647

  Fragments arranged by size: 

                2647     826     559     360     153      89      87      87
                  81      66
 
RsrII CG'GwC_CG

Cuts at:   3150    3150 
   Size:       4955
 
SacI G_AGCT'C

Cuts at:   1820    1969    1820 
   Size:        149    4806
 
SacII CC_GC'GG

Cuts at:    298     926     298 
   Size:        628    4327
 
SalI G'TCGA_C

Cuts at:   1992    1992 
   Size:       4955
 
SanDI GG'GwC_CC

Cuts at:    473     473 
   Size:       4955
 
SapI GCTCTTCn'nnn_

Cuts at:    847    2978    3188     847 
   Size:       2131     210    2614

  Fragments arranged by size: 

                2614    2131     210
 
SbfI CC_TGCA'GG

Cuts at:   2002    2002 
   Size:       4955
 
ScaI AGT'ACT

Cuts at:   4472    4472 
   Size:       4955
 
SexAI A'CCwGG_T

Cuts at:   1925    1925 
   Size:       4955
 
SfcI C'TryA_G

Cuts at:     33     163    1232    1254    1998    2683    3676    3867      33 
   Size:        130    1069      22     744     685     993     191    1121

  Fragments arranged by size: 

                1121    1069     993     744     685     191     130      22
            
 
SfoI GGC'GCC

Cuts at:   1606    2387    2635    1606 
   Size:        781     248    3926

  Fragments arranged by size: 

                3926     781     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4955
 
SmaI CCC'GGG

Cuts at:   1593    1977    1593 
   Size:        384    4571
 
SmlI C'TyrA_G

Cuts at:   2110    3025    3517    3779    2110 
   Size:        915     492     262    3286

  Fragments arranged by size: 

                3286     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1947    1947 
   Size:       4955
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4955
 
SphI G_CATG'C

Cuts at:   1831    2008    2508    1831 
   Size:        177     500    4278

  Fragments arranged by size: 

                4278     500     177
 
StyI C'CwwG_G

Cuts at:    125    1022    2089    2443     125 
   Size:        897    1067     354    2637

  Fragments arranged by size: 

                2637    1067     897     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    199    1558    1852    2538    2671    3130    3339     199 
   Size:       1359     294     686     133     459     209    1815

  Fragments arranged by size: 

                1815    1359     686     459     294     209     133
 
TatI w'GTAC_w

Cuts at:    546    4470     546 
   Size:       3924    1031
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4472    4504    4472 
   Size:         32    4923
 
Tth111I GACn'n_nGTC

Cuts at:   2752    2752 
   Size:       4955
 
XbaI T'CTAG_A

Cuts at:   1986    1986 
   Size:       4955
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    580    2450     580 
   Size:       1870    3085
 
XmaI C'CCGG_G

Cuts at:   1591    1975    1591 
   Size:        384    4571
 
XmnI GAAnn'nnTTC

Cuts at:   1798    1882    1798 
   Size:         84    4871
 
ZraI GAC'GTC

Cuts at:   1721    1721 
   Size:       4955
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AloI     AscI    AvrII     BaeI     BbsI    BbvCI
     BcgI    BfrBI    BmgBI     BmtI    BsiWI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI    PshAI     PsiI    PspXI     PsrI     SfiI    SgrAI
     SrfI     SspI     StuI     SwaI     XhoI
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