Restriction map for pF1KSDA0048
 (Linear) (Six-base) MAPSORT of: KIAA0048.seq  Check: 8933  from: 1  to: 1818



KIAA0048.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     759     903    1818 
   Size:        759     144     915

  Fragments arranged by size: 

                 915     759     144
 
AatII G_ACGT'C

Cuts at:      0    1597    1818 
   Size:       1597     221
 
AccI GT'mk_AC

Cuts at:      0    1040    1818 
   Size:       1040     778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244    1818 
   Size:        244    1574
 
AfeI AGC'GCT

Cuts at:      0    1756    1818 
   Size:       1756      62
 
AflIII A'CryG_T

Cuts at:      0     100    1495    1818 
   Size:        100    1395     323

  Fragments arranged by size: 

                1395     323     100
 
AleI CACnn'nnGTG

Cuts at:      0     556    1818 
   Size:        556    1262
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1202    1818 
   Size:       1202     616
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1362    1818 
   Size:        939     423     456

  Fragments arranged by size: 

                 939     456     423
 
ApaI G_GGCC'C

Cuts at:      0     660    1818 
   Size:        660    1158
 
AvaI C'yCGr_G

Cuts at:      0    1465    1818 
   Size:       1465     353
 
BanI G'GyrC_C

Cuts at:      0     460     888    1478    1818 
   Size:        460     428     590     340

  Fragments arranged by size: 

                 590     460     428     340
 
BanII G_rGCy'C

Cuts at:      0     660     847    1414    1694    1818 
   Size:        660     187     567     280     124

  Fragments arranged by size: 

                 660     567     280     187     124
 
BbeI G_GCGC'C

Cuts at:      0    1482    1818 
   Size:       1482     336
 
BclI T'GATC_A

Cuts at:      0     335    1818 
   Size:        335    1483
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1264    1818 
   Size:       1264     554
 
BlpI GC'TnA_GC

Cuts at:      0     764    1019    1408    1818 
   Size:        764     255     389     410

  Fragments arranged by size: 

                 764     410     389     255
 
Bme1580I G_kGCm'C

Cuts at:      0     660     893    1611    1818 
   Size:        660     233     718     207

  Fragments arranged by size: 

                 718     660     233     207
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20    1254    1737    1818 
   Size:         20    1234     483      81

  Fragments arranged by size: 

                1234     483      81      20
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     317    1550    1818 
   Size:        104     213    1233     268

  Fragments arranged by size: 

                1233     268     213     104
 
Bpu10I CC'TnA_GC

Cuts at:      0      81    1818 
   Size:         81    1737
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     784    1450    1818 
   Size:        784     666     368

  Fragments arranged by size: 

                 784     666     368
 
BsaAI yAC'GTr

Cuts at:      0    1496    1818 
   Size:       1496     322
 
BsaBI GATnn'nnATC

Cuts at:      0     448    1818 
   Size:        448    1370
 
BsaHI Gr'CG_yC

Cuts at:      0    1479    1594    1818 
   Size:       1479     115     224

  Fragments arranged by size: 

                1479     224     115
 
BsaWI w'CCGG_w

Cuts at:      0    1057    1818 
   Size:       1057     761
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1510    1540    1818 
   Size:       1510      30     278

  Fragments arranged by size: 

                1510     278      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     661    1779    1818 
   Size:        661    1118      39

  Fragments arranged by size: 

                1118     661      39
 
BseYI C'CCAG_C

Cuts at:      0      68     743     929    1095    1227    1460    1818 
   Size:         68     675     186     166     132     233     358

  Fragments arranged by size: 

                 675     358     233     186     166     132      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     970    1087    1390    1476    1606    1818 
   Size:        580     390     117     303      86     130     212

  Fragments arranged by size: 

                 580     390     303     212     130     117      86
 
BsiHKAI G_wGCw'C

Cuts at:      0    1152    1694    1818 
   Size:       1152     542     124

  Fragments arranged by size: 

                1152     542     124
 
BsmI GAATG_Cn'

Cuts at:      0    1257    1818 
   Size:       1257     561
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     769    1818 
   Size:        769    1049
 
Bsp1286I G_dGCh'C

Cuts at:      0     660     847     893    1152    1414    1611    1694    1818 
   Size:        660     187      46     259     262     197      83     124

  Fragments arranged by size: 

                 660     262     259     197     187     124      83      46
            
 
BspHI T'CATG_A

Cuts at:      0    1760    1818 
   Size:       1760      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      44     201     759     836     867     903    1818 
   Size:         44     157     558      77      31      36     915

  Fragments arranged by size: 

                 915     558     157      77      44      36      31
 
BsrBI CCG'CTC

Cuts at:      0     566    1635    1818 
   Size:        566    1069     183

  Fragments arranged by size: 

                1069     566     183
 
BsrDI GCAATG_nn'

Cuts at:      0     609    1289    1818 
   Size:        609     680     529

  Fragments arranged by size: 

                 680     609     529
 
BssSI C'ACGA_G

Cuts at:      0     634    1818 
   Size:        634    1184
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     939    1105    1147    1490    1818 
   Size:        939     166      42     343     328

  Fragments arranged by size: 

                 939     343     328     166      42
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1162    1818 
   Size:       1162     656
 
BstYI r'GATC_y

Cuts at:      0     443     923    1007    1421    1818 
   Size:        443     480      84     414     397

  Fragments arranged by size: 

                 480     443     414     397      84
 
BtgI C'CryG_G

Cuts at:      0     169     797    1818 
   Size:        169     628    1021

  Fragments arranged by size: 

                1021     628     169
 
BtsI GCAGTG_nn'

Cuts at:      0       8    1818 
   Size:          8    1810
 
DraIII CAC_nnn'GTG

Cuts at:      0    1684    1818 
   Size:       1684     134
 
EaeI y'GGCC_r

Cuts at:      0    1448    1818 
   Size:       1448     370
 
EarI CTCTTCn'nnn_

Cuts at:      0     721    1516    1663    1818 
   Size:        721     795     147     155

  Fragments arranged by size: 

                 795     721     155     147
 
EcoICRI GAG'CTC

Cuts at:      0    1692    1818 
   Size:       1692     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     244     317    1550    1818 
   Size:        104     140      73    1233     268

  Fragments arranged by size: 

                1233     268     140     104      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1081    1818 
   Size:       1081     737
 
EcoO109I rG'GnC_Cy

Cuts at:      0     347     656     657    1818 
   Size:        347     309       1    1161

  Fragments arranged by size: 

                1161     347     309       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     243    1614    1818 
   Size:        243    1371     204

  Fragments arranged by size: 

                1371     243     204
 
HaeII r_GCGC'y

Cuts at:      0     287    1482    1758    1818 
   Size:        287    1195     276      60

  Fragments arranged by size: 

                1195     287     276      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     600     632    1017    1049    1510    1542    1818 
   Size:        600      32     385      32     461      32     276

  Fragments arranged by size: 

                 600     461     385     276      32      32      32
 
HincII GTy'rAC

Cuts at:      0     587     627    1818 
   Size:        587      40    1191

  Fragments arranged by size: 

                1191     587      40
 
KasI G'GCGC_C

Cuts at:      0    1478    1818 
   Size:       1478     340
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1719    1818 
   Size:       1719      99
 
MslI CAynn'nnrTG

Cuts at:      0     556    1160    1818 
   Size:        556     604     658

  Fragments arranged by size: 

                 658     604     556
 
MspA1I CmG'CkG

Cuts at:      0     171     306     799     865    1018    1105    1186    1273 
   Size:        171     135     493      66     153      87      81      87

Cuts at:   1273    1362    1428    1818 
   Size:         89      66     390

  Fragments arranged by size: 

                 493     390     171     153     135      89      87      87
                  81      66      66
 
NarI GG'CG_CC

Cuts at:      0    1479    1818 
   Size:       1479     339
 
NspI r_CATG'y

Cuts at:      0     104    1705    1818 
   Size:        104    1601     113

  Fragments arranged by size: 

                1601     113     104
 
PciI A'CATG_T

Cuts at:      0     100    1818 
   Size:        100    1718
 
PfoI T'CCnGG_A

Cuts at:      0     294    1082    1818 
   Size:        294     788     736

  Fragments arranged by size: 

                 788     736     294
 
PmlI CAC'GTG

Cuts at:      0    1496    1818 
   Size:       1496     322
 
PpuMI rG'GwC_Cy

Cuts at:      0     347    1818 
   Size:        347    1471
 
PspOMI G'GGCC_C

Cuts at:      0     656    1818 
   Size:        656    1162
 
PstI C_TGCA'G

Cuts at:      0      41    1110    1818 
   Size:         41    1069     708

  Fragments arranged by size: 

                1069     708      41
 
PvuII CAG'CTG

Cuts at:      0     306     865    1018    1105    1186    1273    1362    1428 
   Size:        306     559     153      87      81      87      89      66

Cuts at:   1428    1818 
   Size:        390

  Fragments arranged by size: 

                 559     390     306     153      89      87      87      81
                  66
 
SacI G_AGCT'C

Cuts at:      0    1694    1818 
   Size:       1694     124
 
SacII CC_GC'GG

Cuts at:      0     172     800    1818 
   Size:        172     628    1018

  Fragments arranged by size: 

                1018     628     172
 
SanDI GG'GwC_CC

Cuts at:      0     347    1818 
   Size:        347    1471
 
SapI GCTCTTCn'nnn_

Cuts at:      0     721    1818 
   Size:        721    1097
 
SexAI A'CCwGG_T

Cuts at:      0    1799    1818 
   Size:       1799      19
 
SfcI C'TryA_G

Cuts at:      0      37    1106    1128    1818 
   Size:         37    1069      22     690

  Fragments arranged by size: 

                1069     690      37      22
 
SfoI GGC'GCC

Cuts at:      0    1480    1818 
   Size:       1480     338
 
SmaI CCC'GGG

Cuts at:      0    1467    1818 
   Size:       1467     351
 
SphI G_CATG'C

Cuts at:      0    1705    1818 
   Size:       1705     113
 
StyI C'CwwG_G

Cuts at:      0     896    1818 
   Size:        896     922
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      73    1432    1726    1818 
   Size:         73    1359     294      92

  Fragments arranged by size: 

                1359     294      92      73
 
TatI w'GTAC_w

Cuts at:      0     420    1818 
   Size:        420    1398
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     454    1818 
   Size:        454    1364
 
XmaI C'CCGG_G

Cuts at:      0    1465    1818 
   Size:       1465     353
 
XmnI GAAnn'nnTTC

Cuts at:      0    1672    1756    1818 
   Size:       1672      84      62

  Fragments arranged by size: 

                1672      84      62
 
ZraI GAC'GTC

Cuts at:      0    1595    1818 
   Size:       1595     223
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AleI     AlfI
    AlwNI     ApaI     AvaI     BanI    BanII     BbeI     BclI     BglI
     BlpI Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BssSI   BstAPI
    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI
     MmeI     MslI   MspA1I     NarI     NspI     PciI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SapI
    SexAI     SfcI     SfoI     SmaI     SphI     StyI    TaqII     TatI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AhdI     AloI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI    BfrBI    BglII    BmgBI     BmtI     BplI    BpuEI
    BsiEI    BsiWI    BspEI    BsrFI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     MscI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
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