Restriction map for pF1KSDA0046
 (Linear) (Six-base) MAPSORT of: KIAA0046.seq  Check: 178  from: 1  to: 2607



KIAA0046.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      26    2510    2607 
   Size:         26    2484      97

  Fragments arranged by size: 

                2484      97      26
 
AccI GT'mk_AC

Cuts at:      0    1835    2607 
   Size:       1835     772
 
Acc65I G'GTAC_C

Cuts at:      0     778    2348    2607 
   Size:        778    1570     259

  Fragments arranged by size: 

                1570     778     259
 
AflIII A'CryG_T

Cuts at:      0    2063    2607 
   Size:       2063     544
 
AleI CACnn'nnGTG

Cuts at:      0    1159    2607 
   Size:       1159    1448
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1358    2607 
   Size:       1358    1249
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2188    2220    2607 
   Size:       2188      32     387

  Fragments arranged by size: 

                2188     387      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1860    2127    2607 
   Size:       1860     267     480

  Fragments arranged by size: 

                1860     480     267
 
ApaLI G'TGCA_C

Cuts at:      0    1096    2607 
   Size:       1096    1511
 
ApoI r'AATT_y

Cuts at:      0    1263    2566    2607 
   Size:       1263    1303      41

  Fragments arranged by size: 

                1303    1263      41
 
AvaI C'yCGr_G

Cuts at:      0      76     478    1997    2607 
   Size:         76     402    1519     610

  Fragments arranged by size: 

                1519     610     402      76
 
BanI G'GyrC_C

Cuts at:      0     510     765     778    2348    2607 
   Size:        510     255      13    1570     259

  Fragments arranged by size: 

                1570     510     259     255      13
 
BanII G_rGCy'C

Cuts at:      0     584     827    1664    1820    2607 
   Size:        584     243     837     156     787

  Fragments arranged by size: 

                 837     787     584     243     156
 
BbeI G_GCGC'C

Cuts at:      0     769    2607 
   Size:        769    1838
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     414    2189    2460    2607 
   Size:        414    1775     271     147

  Fragments arranged by size: 

                1775     414     271     147
 
BbvCI CC'TCA_GC

Cuts at:      0    2584    2607 
   Size:       2584      23
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2475    2509    2607 
   Size:       2475      34      98

  Fragments arranged by size: 

                2475      98      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     467    2607 
   Size:        467    2140
 
BglI GCCn_nnn'nGGC

Cuts at:      0     368    1748    2329    2607 
   Size:        368    1380     581     278

  Fragments arranged by size: 

                1380     581     368     278
 
Bme1580I G_kGCm'C

Cuts at:      0    1100    2607 
   Size:       1100    1507
 
BmgBI CAC'GTC

Cuts at:      0    2457    2607 
   Size:       2457     150
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     655    2159    2607 
   Size:        655    1504     448

  Fragments arranged by size: 

                1504     655     448
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     764    1228    1336    2607 
   Size:        596     168     464     108    1271

  Fragments arranged by size: 

                1271     596     464     168     108
 
Bpu10I CC'TnA_GC

Cuts at:      0    1109    2584    2607 
   Size:       1109    1475      23

  Fragments arranged by size: 

                1475    1109      23
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    2607 
   Size:        307    2300
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      23      58     190     289    2607 
   Size:         23      35     132      99    2318

  Fragments arranged by size: 

                2318     132      99      35      23
 
BsaAI yAC'GTr

Cuts at:      0     830    1737    2607 
   Size:        830     907     870

  Fragments arranged by size: 

                 907     870     830
 
BsaBI GATnn'nnATC

Cuts at:      0     158    2607 
   Size:        158    2449
 
BsaHI Gr'CG_yC

Cuts at:      0     766    2607 
   Size:        766    1841
 
BsaWI w'CCGG_w

Cuts at:      0     530    2607 
   Size:        530    2077
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     790     820    2188    2218    2516    2546    2607 
   Size:        790      30    1368      30     298      30      61

  Fragments arranged by size: 

                1368     790     298      61      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     124     127     367     811    2104    2212    2282 
   Size:         94      30       3     240     444    1293     108      70

Cuts at:   2282    2607 
   Size:        325

  Fragments arranged by size: 

                1293     444     325     240     108      94      70      30
                   3
 
BseYI C'CCAG_C

Cuts at:      0     905    2607 
   Size:        905    1702
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     252     378    1595    2607 
   Size:         30     222     126    1217    1012

  Fragments arranged by size: 

                1217    1012     222     126      30
 
BsiEI CG_ry'CG

Cuts at:      0    2242    2607 
   Size:       2242     365
 
BsiHKAI G_wGCw'C

Cuts at:      0     842     958    1100    2078    2607 
   Size:        842     116     142     978     529

  Fragments arranged by size: 

                 978     842     529     142     116
 
BsmI GAATG_Cn'

Cuts at:      0    1198    2607 
   Size:       1198    1409
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     532    2445    2463    2607 
   Size:        532    1913      18     144

  Fragments arranged by size: 

                1913     532     144      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     584     827     842     958    1100    1664    1820    2078 
   Size:        584     243      15     116     142     564     156     258

Cuts at:   2078    2607 
   Size:        529

  Fragments arranged by size: 

                 584     564     529     258     243     156     142     116
                  15
 
BspEI T'CCGG_A

Cuts at:      0     530    2607 
   Size:        530    2077
 
BspHI T'CATG_A

Cuts at:      0    1937    2607 
   Size:       1937     670
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      26     454    2510    2607 
   Size:         26     428    2056      97

  Fragments arranged by size: 

                2056     428      97      26
 
BsrBI CCG'CTC

Cuts at:      0     736    2202    2607 
   Size:        736    1466     405

  Fragments arranged by size: 

                1466     736     405
 
BsrFI r'CCGG_y

Cuts at:      0     453    2607 
   Size:        453    2154
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      41    2124    2607 
   Size:         41    2083     483

  Fragments arranged by size: 

                2083     483      41
 
BstEII G'GTnAC_C

Cuts at:      0    1149    2607 
   Size:       1149    1458
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     925    1161    1369    2607 
   Size:        925     236     208    1238

  Fragments arranged by size: 

                1238     925     236     208
 
BstYI r'GATC_y

Cuts at:      0     671    2607 
   Size:        671    1936
 
BtgI C'CryG_G

Cuts at:      0     200    1157    1880    1917    2192    2223    2344    2507 
   Size:        200     957     723      37     275      31     121     163

Cuts at:   2507    2607 
   Size:        100

  Fragments arranged by size: 

                 957     723     275     200     163     121     100      37
                  31
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2064    2607 
   Size:       2064     543
 
EaeI y'GGCC_r

Cuts at:      0    1313    1688    2330    2607 
   Size:       1313     375     642     277

  Fragments arranged by size: 

                1313     642     375     277
 
EarI CTCTTCn'nnn_

Cuts at:      0     316     589    1215    1327    1363    2607 
   Size:        316     273     626     112      36    1244

  Fragments arranged by size: 

                1244     626     316     273     112      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2197    2607 
   Size:       2197     410
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     764    1228    1336    2607 
   Size:        596     168     464     108    1271

  Fragments arranged by size: 

                1271     596     464     168     108
 
EcoO109I rG'GnC_Cy

Cuts at:      0     549     601    1032    1556    2109    2607 
   Size:        549      52     431     524     553     498

  Fragments arranged by size: 

                 553     549     524     498     431      52
 
EcoRI G'AATT_C

Cuts at:      0    1263    2607 
   Size:       1263    1344
 
EcoRV GAT'ATC

Cuts at:      0    1731    2607 
   Size:       1731     876
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     735    2607 
   Size:        735    1872
 
FspI TGC'GCA

Cuts at:      0    1876    2607 
   Size:       1876     731
 
HaeII r_GCGC'y

Cuts at:      0     769    2300    2607 
   Size:        769    1531     307

  Fragments arranged by size: 

                1531     769     307
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     709     741     790     822    2607 
   Size:        709      32      49      32    1785

  Fragments arranged by size: 

                1785     709      49      32      32
 
KasI G'GCGC_C

Cuts at:      0     765    2607 
   Size:        765    1842
 
KpnI G_GTAC'C

Cuts at:      0     782    2352    2607 
   Size:        782    1570     255

  Fragments arranged by size: 

                1570     782     255
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1411    2607 
   Size:       1411    1196
 
MscI TGG'CCA

Cuts at:      0    1315    1690    2607 
   Size:       1315     375     917

  Fragments arranged by size: 

                1315     917     375
 
MslI CAynn'nnrTG

Cuts at:      0    1159    1367    1681    2398    2607 
   Size:       1159     208     314     717     209

  Fragments arranged by size: 

                1159     717     314     209     208
 
MspA1I CmG'CkG

Cuts at:      0    1579    1919    2346    2607 
   Size:       1579     340     427     261

  Fragments arranged by size: 

                1579     427     340     261
 
NarI GG'CG_CC

Cuts at:      0     766    2607 
   Size:        766    1841
 
NcoI C'CATG_G

Cuts at:      0     200    2192    2607 
   Size:        200    1992     415

  Fragments arranged by size: 

                1992     415     200
 
NspI r_CATG'y

Cuts at:      0    1430    2067    2607 
   Size:       1430     637     540

  Fragments arranged by size: 

                1430     637     540
 
PciI A'CATG_T

Cuts at:      0    2063    2607 
   Size:       2063     544
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1860    2367    2607 
   Size:       1860     507     240

  Fragments arranged by size: 

                1860     507     240
 
PfoI T'CCnGG_A

Cuts at:      0     469    2607 
   Size:        469    2138
 
PmlI CAC'GTG

Cuts at:      0     830    1737    2607 
   Size:        830     907     870

  Fragments arranged by size: 

                 907     870     830
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1918    1950    2188    2220    2607 
   Size:       1918      32     238      32     387

  Fragments arranged by size: 

                1918     387     238      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     549    1556    2109    2607 
   Size:        549    1007     553     498

  Fragments arranged by size: 

                1007     553     549     498
 
PshAI GACnn'nnGTC

Cuts at:      0    1203    1831    2607 
   Size:       1203     628     776

  Fragments arranged by size: 

                1203     776     628
 
PstI C_TGCA'G

Cuts at:      0      37     756    2120    2576    2607 
   Size:         37     719    1364     456      31

  Fragments arranged by size: 

                1364     719     456      37      31
 
SacII CC_GC'GG

Cuts at:      0    1920    2347    2607 
   Size:       1920     427     260

  Fragments arranged by size: 

                1920     427     260
 
SapI GCTCTTCn'nnn_

Cuts at:      0     589    1363    2607 
   Size:        589     774    1244

  Fragments arranged by size: 

                1244     774     589
 
SfcI C'TryA_G

Cuts at:      0      33     752    2116    2572    2607 
   Size:         33     719    1364     456      35

  Fragments arranged by size: 

                1364     719     456      35      33
 
SfoI GGC'GCC

Cuts at:      0     767    2607 
   Size:        767    1840
 
SmaI CCC'GGG

Cuts at:      0    1999    2607 
   Size:       1999     608
 
SmlI C'TyrA_G

Cuts at:      0     322    2607 
   Size:        322    2285
 
StyI C'CwwG_G

Cuts at:      0      93     130     200     552     819    2192    2607 
   Size:         93      37      70     352     267    1373     415

  Fragments arranged by size: 

                1373     415     352     267      93      70      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     331    2228    2467    2607 
   Size:        331    1897     239     140

  Fragments arranged by size: 

                1897     331     239     140
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2052    2084    2516    2548    2607 
   Size:       2052      32     432      32      59

  Fragments arranged by size: 

                2052     432      59      32      32
 
XbaI T'CTAG_A

Cuts at:      0     792    2607 
   Size:        792    1815
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1809    2183    2607 
   Size:       1809     374     424

  Fragments arranged by size: 

                1809     424     374
 
XmaI C'CCGG_G

Cuts at:      0    1997    2607 
   Size:       1997     610
 
XmnI GAAnn'nnTTC

Cuts at:      0    1933    2607 
   Size:       1933     674
 

 Enzymes that do cut:

     AarI     AccI   Acc65I   AflIII     AleI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BglI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I     KasI     KpnI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NspI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI    PshAI     PstI    SacII     SapI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TstI     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI    AflII     AgeI     AhdI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI    BsiWI    BsrDI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI     FseI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XhoI
     ZraI
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