Restriction map for pF1KSDA0039
 (Linear) (Six-base) MAPSORT of: KIAA0039.seq  Check: 2229  from: 1  to: 1398



KIAA0039.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     546    1398 
   Size:        546     852
 
AccI GT'mk_AC

Cuts at:      0    1211    1398 
   Size:       1211     187
 
AflIII A'CryG_T

Cuts at:      0     482    1152    1398 
   Size:        482     670     246

  Fragments arranged by size: 

                 670     482     246
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     805     868    1398 
   Size:        120     685      63     530

  Fragments arranged by size: 

                 685     530     120      63
 
ApaI G_GGCC'C

Cuts at:      0    1327    1398 
   Size:       1327      71
 
ApoI r'AATT_y

Cuts at:      0     153     391     736    1398 
   Size:        153     238     345     662

  Fragments arranged by size: 

                 662     345     238     153
 
BanI G'GyrC_C

Cuts at:      0     681    1398 
   Size:        681     717
 
BanII G_rGCy'C

Cuts at:      0     165     432    1123    1327    1398 
   Size:        165     267     691     204      71

  Fragments arranged by size: 

                 691     267     204     165      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     443    1264    1398 
   Size:        443     821     134

  Fragments arranged by size: 

                 821     443     134
 
BfrBI ATG'CAT

Cuts at:      0     661    1398 
   Size:        661     737
 
BlpI GC'TnA_GC

Cuts at:      0      76    1398 
   Size:         76    1322
 
Bme1580I G_kGCm'C

Cuts at:      0    1327    1398 
   Size:       1327      71
 
BmtI G_CTAG'C

Cuts at:      0     277    1398 
   Size:        277    1121
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1107    1398 
   Size:       1107     291
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     975    1046    1049    1258    1398 
   Size:        975      71       3     209     140

  Fragments arranged by size: 

                 975     209     140      71       3
 
BseYI C'CCAG_C

Cuts at:      0     557    1398 
   Size:        557     841
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431    1212    1398 
   Size:        431     781     186

  Fragments arranged by size: 

                 781     431     186
 
BsiHKAI G_wGCw'C

Cuts at:      0     432    1123    1398 
   Size:        432     691     275

  Fragments arranged by size: 

                 691     432     275
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1070    1398 
   Size:       1070     328
 
Bsp1286I G_dGCh'C

Cuts at:      0     165     432    1123    1327    1398 
   Size:        165     267     691     204      71

  Fragments arranged by size: 

                 691     267     204     165      71
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     540     546    1398 
   Size:        540       6     852

  Fragments arranged by size: 

                 852     540       6
 
BsrGI T'GTAC_A

Cuts at:      0     299    1398 
   Size:        299    1099
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     832    1398 
   Size:        832     566
 
BtsI GCAGTG_nn'

Cuts at:      0     266     773     999    1398 
   Size:        266     507     226     399

  Fragments arranged by size: 

                 507     399     266     226
 
DraI TTT'AAA

Cuts at:      0     741    1398 
   Size:        741     657
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      37    1398 
   Size:         37    1361
 
EarI CTCTTCn'nnn_

Cuts at:      0     450    1235    1398 
   Size:        450     785     163

  Fragments arranged by size: 

                 785     450     163
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1398 
   Size:         19    1379
 
EcoICRI GAG'CTC

Cuts at:      0     430    1121    1398 
   Size:        430     691     277

  Fragments arranged by size: 

                 691     430     277
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1323    1324    1398 
   Size:       1323       1      74

  Fragments arranged by size: 

                1323      74       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1264    1398 
   Size:       1264     134
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1030    1062    1207    1239    1329    1361    1398 
   Size:       1030      32     145      32      90      32      37

  Fragments arranged by size: 

                1030     145      90      37      32      32      32
 
HincII GTy'rAC

Cuts at:      0     102    1398 
   Size:        102    1296
 
HpaI GTT'AAC

Cuts at:      0     102    1398 
   Size:        102    1296
 
MluI A'CGCG_T

Cuts at:      0    1152    1398 
   Size:       1152     246
 
MslI CAynn'nnrTG

Cuts at:      0      69     295    1291    1398 
   Size:         69     226     996     107

  Fragments arranged by size: 

                 996     226     107      69
 
MspA1I CmG'CkG

Cuts at:      0     415    1398 
   Size:        415     983
 
NheI G'CTAG_C

Cuts at:      0     273    1398 
   Size:        273    1125
 
NsiI A_TGCA'T

Cuts at:      0     663    1398 
   Size:        663     735
 
NspI r_CATG'y

Cuts at:      0     175     486    1398 
   Size:        175     311     912

  Fragments arranged by size: 

                 912     311     175
 
PciI A'CATG_T

Cuts at:      0     482    1398 
   Size:        482     916
 
PshAI GACnn'nnGTC

Cuts at:      0    1368    1398 
   Size:       1368      30
 
PspOMI G'GGCC_C

Cuts at:      0    1323    1398 
   Size:       1323      75
 
PstI C_TGCA'G

Cuts at:      0     388     669     837     884    1398 
   Size:        388     281     168      47     514

  Fragments arranged by size: 

                 514     388     281     168      47
 
PvuII CAG'CTG

Cuts at:      0     415    1398 
   Size:        415     983
 
SacI G_AGCT'C

Cuts at:      0     432    1123    1398 
   Size:        432     691     275

  Fragments arranged by size: 

                 691     432     275
 
SbfI CC_TGCA'GG

Cuts at:      0     837    1398 
   Size:        837     561
 
SfcI C'TryA_G

Cuts at:      0     384     665     797     833     880    1398 
   Size:        384     281     132      36      47     518

  Fragments arranged by size: 

                 518     384     281     132      47      36
 
SmlI C'TyrA_G

Cuts at:      0    1122    1398 
   Size:       1122     276
 
StuI AGG'CCT

Cuts at:      0     839    1398 
   Size:        839     559
 
TatI w'GTAC_w

Cuts at:      0     299    1269    1398 
   Size:        299     970     129

  Fragments arranged by size: 

                 970     299     129
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     573    1398 
   Size:        573     825
 
XmnI GAAnn'nnTTC

Cuts at:      0    1007    1398 
   Size:       1007     391
 

 Enzymes that do cut:

     AarI     AccI   AflIII    AlwNI     ApaI     ApoI     BanI    BanII
     BbsI    BfrBI     BlpI Bme1580I     BmtI    BpuEI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrGI   BstAPI     BtsI
     DraI     DrdI     EarI     EciI  EcoICRI EcoO109I     FalI    Hin4I
   HincII     HpaI     MluI     MslI   MspA1I     NheI     NsiI     NspI
     PciI    PshAI   PspOMI     PstI    PvuII     SacI     SbfI     SfcI
     SmlI     StuI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
     BglI    BglII    BmgBI     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     EaeI     EagI  Eco57MI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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