Restriction map for pF1KSDA0036
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

Acc65I      AccII       AccIII      AciI        AflII       
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FauI        FseI        
FspI        HapII       HgaI        HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MluI        MluNI       MroI        MscI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SwaI        Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         773  GACGTC               GACGT^C
AccI          776  GTCTAC               GT^[AC][GT]AC
AclI         1549  AACGTT               AA^CGTT
AcyI          773  GACGTC               G[GA]^CG[CT]C
AflIII        370  ACATGT               A^C[GA][CT]GT
Aor51HI      1278  AGCGCT               AGC^GCT
ApaI         1490  GGGCCC               GGGCC^C
AvaI         1088  CCCGAG               C^[CT]CG[GA]G
BanI         1569  GGCACC               G^G[CT][GA]CC
BbsI         1400  GAAGAC               GAAGAC(2/6)
BclI          498  TGATCA               T^GATCA
BglI         1645  GCCAAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        1400  GAAGAC               GAAGAC(2/6)
BsaHI         773  GACGTC               G[GA]^CG[CT]C
BseRI         975  GAGGAG               GAGGAG(10/8)
BsiYI        1327  CCTTGGCGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1327  CCTTGGCGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI        1088  CCCGAG               C^[CT]CG[GA]G
BspHI         551  TCATGA               T^CATGA
BspLU11I      370  ACATGT               A^CATGT
BspMI         754  ACCTGC               ACCTGC(4/8)
BsrDI         265  CATTGC               GCAATG(2/0)
BstXI        1625  CCAGGCCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1279  GCGC                 GCG^C
DrdI          767  GACTCAGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1278  AGCGCT               AGC^GCT
EcoNI        1327  CCTTGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         805  GAATTC               G^AATTC
FbaI          498  TGATCA               T^GATCA
HaeII        1278  AGCGCT               [GA]GCGC^[CT]
HhaI         1279  GCGC                 GCG^C
Hin1I         773  GACGTC               G[GA]^CG[CT]C
HinP1I       1279  GCGC                 G^CGC
MfeI         1051  CAATTG               C^AATTG
MunI         1051  CAATTG               C^AATTG
PpuMI        1037  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     1549  AACGTT               AA^CGTT
RcaI          551  TCATGA               T^CATGA
ScaI          682  AGTACT               AGT^ACT
SfiI         1644  GGCCAAGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1457  CTCAAG               C^T[CT][GA]AG
SphI          892  GCATGC               GCATG^C
SspI          179  AATATT               AAT^ATT
StuI         1627  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AspHI        1441  GAGCTC               G[AT]GC[AT]^C
             1606  GAGCTC              
AspI          526  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1390  GACTATGTC           
AvaII         192  GGTCC                G^G[AT]CC
             1038  GGACC               
BpmI         1009  CTGGAG               CTGGAG(16/14)
             1715  CTGGAG              
BsiHKAI      1441  GAGCTC               G[AT]GC[AT]^C
             1606  GAGCTC              
BsmFI         799  GGGAC                GGGAC(10/14)
               73  GTCCC               
BsrI          796  ACTGG                ACTGG(1/-1)
              670  CCAGT               
HphI          589  GGTGA                GGTGA(8/7)
             1221  TCACC               
MaeII         774  ACGT                 A^CGT
             1550  ACGT                
MaeIII        433  GTGAC                ^GT[ACGT]AC
             1261  GTCAC               
NspI          370  ACATGT               [GA]CATG^[CT]
              892  GCATGC              
PflFI         526  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1390  GACTATGTC           
PleI          767  GACTC                GAGTC(4/5)
             1337  GACTC               
PstI          332  CTGCAG               CTGCA^G
              545  CTGCAG              
SacI         1441  GAGCTC               GAGCT^C
             1606  GAGCTC              
TaqI          213  TCGA                 T^CGA
             1112  TCGA                
Tsp45I        433  GTGAC                ^GT[GC]AC
             1261  GTCAC               
Tth111I       526  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1390  GACTATGTC           
TthHB8I       213  TCGA                 T^CGA
             1112  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     BanII       BbvI        BfaI        BmyI        
BsaJI       BsmAI       Bsp1286I    BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HindIII     
HinfI       ItaI        Ksp632I     MaeI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PvuII       RsaI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com