Restriction map for pF1KSDA0036
 (Linear) (Six-base) MAPSORT of: KIAA0036.seq  Check: 355  from: 1  to: 1794



KIAA0036.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AatII G_ACGT'C

Cuts at:      0     777    1794 
   Size:        777    1017
 
AccI GT'mk_AC

Cuts at:      0     777    1794 
   Size:        777    1017
 
AclI AA'CG_TT

Cuts at:      0    1550    1794 
   Size:       1550     244
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     416    1393    1600    1690    1794 
   Size:         65     351     977     207      90     104

  Fragments arranged by size: 

                 977     351     207     104      90      65
 
AfeI AGC'GCT

Cuts at:      0    1280    1794 
   Size:       1280     514
 
AflIII A'CryG_T

Cuts at:      0     370    1794 
   Size:        370    1424
 
AleI CACnn'nnGTG

Cuts at:      0    1689    1794 
   Size:       1689     105
 
ApaI G_GGCC'C

Cuts at:      0    1494    1794 
   Size:       1494     300
 
ApoI r'AATT_y

Cuts at:      0     304     339     728     805    1215    1285    1764    1794 
   Size:        304      35     389      77     410      70     479      30

  Fragments arranged by size: 

                 479     410     389     304      77      70      35      30
            
 
AvaI C'yCGr_G

Cuts at:      0    1088    1794 
   Size:       1088     706
 
BanI G'GyrC_C

Cuts at:      0    1569    1794 
   Size:       1569     225
 
BanII G_rGCy'C

Cuts at:      0    1414    1445    1494    1509    1610    1794 
   Size:       1414      31      49      15     101     184

  Fragments arranged by size: 

                1414     184     101      49      31      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1407    1794 
   Size:       1407     387
 
BclI T'GATC_A

Cuts at:      0     498    1794 
   Size:        498    1296
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1651    1794 
   Size:       1651     143
 
Bme1580I G_kGCm'C

Cuts at:      0    1494    1794 
   Size:       1494     300
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     806    1794 
   Size:        806     988
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1253    1794 
   Size:       1253     541
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1030    1736    1794 
   Size:       1030     706      58

  Fragments arranged by size: 

                1030     706      58
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     389    1604    1794 
   Size:         62     327    1215     190

  Fragments arranged by size: 

                1215     327     190      62
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1442    1794 
   Size:       1442     352
 
BsaHI Gr'CG_yC

Cuts at:      0     774    1794 
   Size:        774    1020
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     452     482    1794 
   Size:        452      30    1312

  Fragments arranged by size: 

                1312     452      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     990    1794 
   Size:        990     804
 
BseYI C'CCAG_C

Cuts at:      0     242    1508    1794 
   Size:        242    1266     286

  Fragments arranged by size: 

                1266     286     242
 
BsiHKAI G_wGCw'C

Cuts at:      0    1445    1610    1794 
   Size:       1445     165     184

  Fragments arranged by size: 

                1445     184     165
 
Bsp1286I G_dGCh'C

Cuts at:      0    1414    1445    1494    1509    1610    1794 
   Size:       1414      31      49      15     101     184

  Fragments arranged by size: 

                1414     184     101      49      31      15
 
BspHI T'CATG_A

Cuts at:      0     551    1794 
   Size:        551    1243
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     763    1794 
   Size:        763    1031
 
BsrDI GCAATG_nn'

Cuts at:      0     264    1794 
   Size:        264    1530
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1632    1794 
   Size:       1632     162
 
BstYI r'GATC_y

Cuts at:      0      26     541     985    1621    1794 
   Size:         26     515     444     636     173

  Fragments arranged by size: 

                 636     515     444     173      26
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     773    1794 
   Size:        773    1021
 
EarI CTCTTCn'nnn_

Cuts at:      0     451     863    1028    1154    1493    1615    1794 
   Size:        451     412     165     126     339     122     179

  Fragments arranged by size: 

                 451     412     339     179     165     126     122
 
EcoICRI GAG'CTC

Cuts at:      0    1443    1608    1794 
   Size:       1443     165     186

  Fragments arranged by size: 

                1443     186     165
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     416    1030    1393    1600    1690    1736    1794 
   Size:         65     351     614     363     207      90      46      58

  Fragments arranged by size: 

                 614     363     351     207      90      65      58      46
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1331    1794 
   Size:       1331     463
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1038    1490    1491    1794 
   Size:       1038     452       1     303

  Fragments arranged by size: 

                1038     452     303       1
 
EcoRI G'AATT_C

Cuts at:      0     805    1794 
   Size:        805     989
 
HaeII r_GCGC'y

Cuts at:      0    1282    1794 
   Size:       1282     512
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     426     458     533     565    1794 
   Size:        426      32      75      32    1229

  Fragments arranged by size: 

                1229     426      75      32      32
 
HindIII A'AGCT_T

Cuts at:      0     649     946    1699    1794 
   Size:        649     297     753      95

  Fragments arranged by size: 

                 753     649     297      95
 
MfeI C'AATT_G

Cuts at:      0    1051    1794 
   Size:       1051     743
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1794 
   Size:        208    1586
 
MslI CAynn'nnrTG

Cuts at:      0     269     556    1689    1794 
   Size:        269     287    1133     105

  Fragments arranged by size: 

                1133     287     269     105
 
MspA1I CmG'CkG

Cuts at:      0     331    1101    1258    1794 
   Size:        331     770     157     536

  Fragments arranged by size: 

                 770     536     331     157
 
NspI r_CATG'y

Cuts at:      0     374     896    1794 
   Size:        374     522     898

  Fragments arranged by size: 

                 898     522     374
 
PciI A'CATG_T

Cuts at:      0     370    1794 
   Size:        370    1424
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1592    1624    1794 
   Size:       1592      32     170

  Fragments arranged by size: 

                1592     170      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1038    1794 
   Size:       1038     756
 
PsiI TTA'TAA

Cuts at:      0     676    1794 
   Size:        676    1118
 
PspOMI G'GGCC_C

Cuts at:      0    1490    1794 
   Size:       1490     304
 
PstI C_TGCA'G

Cuts at:      0     336     549    1794 
   Size:        336     213    1245

  Fragments arranged by size: 

                1245     336     213
 
PvuII CAG'CTG

Cuts at:      0     331    1101    1258    1794 
   Size:        331     770     157     536

  Fragments arranged by size: 

                 770     536     331     157
 
SacI G_AGCT'C

Cuts at:      0    1445    1610    1794 
   Size:       1445     165     184

  Fragments arranged by size: 

                1445     184     165
 
SapI GCTCTTCn'nnn_

Cuts at:      0     863    1493    1615    1794 
   Size:        863     630     122     179

  Fragments arranged by size: 

                 863     630     179     122
 
ScaI AGT'ACT

Cuts at:      0     684    1794 
   Size:        684    1110
 
SfcI C'TryA_G

Cuts at:      0      35     332     545     562    1794 
   Size:         35     297     213      17    1232

  Fragments arranged by size: 

                1232     297     213      35      17
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1651    1794 
   Size:       1651     143
 
SmlI C'TyrA_G

Cuts at:      0    1457    1794 
   Size:       1457     337
 
SphI G_CATG'C

Cuts at:      0     896    1794 
   Size:        896     898
 
SspI AAT'ATT

Cuts at:      0     181    1794 
   Size:        181    1613
 
StuI AGG'CCT

Cuts at:      0    1629    1794 
   Size:       1629     165
 
StyI C'CwwG_G

Cuts at:      0      22    1327    1640    1794 
   Size:         22    1305     313     154

  Fragments arranged by size: 

                1305     313     154      22
 
TatI w'GTAC_w

Cuts at:      0     486     682    1794 
   Size:        486     196    1112

  Fragments arranged by size: 

                1112     486     196
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1163    1195    1794 
   Size:       1163      32     599

  Fragments arranged by size: 

                1163     599      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     529    1393    1794 
   Size:        529     864     401

  Fragments arranged by size: 

                 864     529     401
 
XmnI GAAnn'nnTTC

Cuts at:      0     320     341     476    1118    1794 
   Size:        320      21     135     642     676

  Fragments arranged by size: 

                 676     642     320     135      21
 
ZraI GAC'GTC

Cuts at:      0     775    1794 
   Size:        775    1019
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI     AfeI   AflIII     AleI     ApaI
     ApoI     AvaI     BanI    BanII     BbsI     BclI     BglI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BstXI    BstYI
     DrdI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I     NspI     PciI
     PpiI    PpuMI     PsiI   PspOMI     PstI    PvuII     SacI     SapI
     ScaI     SfcI     SfiI     SmlI     SphI     SspI     StuI     StyI
     TatI     TstI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I    AflII     AgeI     AhdI     AlfI     AloI    AlwNI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BsaI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EaeI     EagI     EciI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PshAI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SwaI    TaqII     XbaI     XcmI     XhoI     XmaI
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