Restriction map for pF1KSDA0033
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfrI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstYI       Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeIII      HapII       
HincII      HindII      HindIII     HpaI        HpaII       
KpnI        Ksp632I     KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        NaeI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcyI           93  GGCGCC               G[GA]^CG[CT]C
AhdI           78  GACGCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          142  GGATC                GGATC(4/5)
AseI          643  ATTAAT               AT^TAAT
AsnI          643  ATTAAT               AT^TAAT
AspEI          78  GACGCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI           93  GGCGCC               GGCGC^C
BbvCI         226  CCTCAGC              CCTCAGC(-5/-2)
BglI          730  GCCATTAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI          93  GGCGCC               G[GA]^CG[CT]C
BsiEI          25  CGATCG               CG[GA][CT]^CG
BspMI         564  GCAGGT               ACCTGC(4/8)
BsrI          517  CCAGT                ACTGG(1/-1)
BstF5I        276  GGATG                GGATG(2/0)
BstXI         266  CCAGAGATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         834  GCCAGC               GC[ACGT]^[ACGT]GC
DsaI           56  CCGTGG               C^C[GA][CT]GG
Eam1105I       78  GACGCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I       284  CCAAGG               C^C[AT][AT]GG
FokI          276  GGATG                GGATG(9/13)
HgaI           78  GACGC                GACGC(5/10)
Hin1I          93  GGCGCC               G[GA]^CG[CT]C
HinfI          14  GAGTC                G^A[ACGT]TC
HphI           42  TCACC                GGTGA(8/7)
KasI           93  GGCGCC               G^GCGCC
MspA1I         71  CAGCGG               C[AC]G^C[GT]G
NarI           93  GGCGCC               GG^CGCC
PleI           14  GAGTC                GAGTC(4/5)
PshBI         643  ATTAAT               AT^TAAT
PstI          188  CTGCAG               CTGCA^G
PvuI           25  CGATCG               CGAT^CG
ScaI          336  AGTACT               AGT^ACT
SfaNI         280  GATGC                GCATC(5/9)
SfcI          188  CTGCAG               C^T[GA][CT]AG
SfoI           93  GGCGCC               GGC^GCC
SspI          795  AATATT               AAT^ATT
StyI          284  CCAAGG               C^C[AT][AT]GG
TspRI         749  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          407  GTATAC               GT^[AC][GT]AC
              523  GTATAC              
AccII          80  CGCG                 CG^CG
               90  CGCG                
AcsI          219  AAATTT               [GA]^AATT[CT]
              865  AAATTT              
AfaI          337  GTAC                 GT^AC
              695  GTAC                
ApoI          219  AAATTT               [GA]^AATT[CT]
              865  AAATTT              
BanI           93  GGCGCC               G^G[CT][GA]CC
              475  GGTGCC              
BsmFI          62  GGGAC                GGGAC(10/14)
               76  GGGAC               
Bst1107I      407  GTATAC               GTA^TAC
              523  GTATAC              
BstUI          80  CGCG                 CG^CG
               90  CGCG                
BstZ17I       407  GTATAC               GTA^TAC
              523  GTATAC              
CfoI           38  GCGC                 GCG^C
               94  GCGC                
FauI           73  GCGGG                CCCGC(4/6)
               81  GCGGG               
HaeII          37  GGCGCT               [GA]GCGC^[CT]
               93  GGCGCC              
HhaI           38  GCGC                 GCG^C
               94  GCGC                
HinP1I         38  GCGC                 G^CGC
               94  GCGC                
MaeII         573  ACGT                 A^CGT
              668  ACGT                
MaeIII        156  GTCAC                ^GT[ACGT]AC
              558  GTCAC               
MvnI           80  CGCG                 CG^CG
               90  CGCG                
RsaI          337  GTAC                 GT^AC
              695  GTAC                
Tsp45I        156  GTCAC                ^GT[GC]AC
              558  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AciI        AluI        BbvI        BfaI        BsaI        
BsaJI       BsiYI       BslI        BsmAI       BssKI       
BstNI       DdeI        DpnII       EcoRII      Fnu4HI      
ItaI        MaeI        MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIV       
Sau3AI      ScrFI       Tru9I       TseI        Tsp509I     
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