Restriction map for pF1KSDA0031
##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AclI        AflII       AhdI        Alw44I      
ApaLI       AscI        AspEI       AspI        AvrII       
BamHI       BfrI        BglI        BlnI        BsiEI       
BsiWI       BsmI        BspDI       BspMI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
FseI        HpaI        KspI        MluI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PpuMI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        


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# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I       1607  GGTACC               G^GTACC
AccI         2446  GTCTAC               GT^[AC][GT]AC
AccII        1058  CGCG                 CG^CG
AccIII       2660  TCCGGA               T^CCGGA
AcyI         1330  GGCGCC               G[GA]^CG[CT]C
AgeI         1360  ACCGGT               A^CCGGT
AocI          223  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       476  AGCGCT               AGC^GCT
ApaI         2328  GGGCCC               GGGCC^C
Asp700I      2344  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1607  GGTACC               G^GTACC
BbeI         1330  GGCGCC               GGCGC^C
BbrPI        2566  CACGTG               CAC^GTG
BbvCI         694  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2849  GCAAATTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI           3  GGATAC               GTATCC(6/5)
BlpI         2065  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2065  GCTGAGC              GC^T[ACGT]AGC
BsaHI        1330  GGCGCC               G[GA]^CG[CT]C
BseAI        2660  TCCGGA               T^CCGGA
BsmFI        2676  GGGAC                GGGAC(10/14)
BspEI        2660  TCCGGA               T^CCGGA
BsrBI         718  GAGCGG               CCGCTC(-3/-3)
BsrDI        1709  GCAATG               GCAATG(2/0)
BstUI        1058  CGCG                 CG^CG
Bsu36I        223  CCTGAGG              CC^T[ACGT]AGG
CelII        2065  GCTGAGC              GC^T[ACGT]AGC
DrdI         1969  GACCCAGTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      476  AGCGCT               AGC^GCT
Eco81I        223  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2794  GAATTC               G^AATTC
EcoT22I      1921  ATGCAT               ATGCA^T
Hin1I        1330  GGCGCC               G[GA]^CG[CT]C
HindIII      2615  AAGCTT               A^AGCTT
KasI         1330  GGCGCC               G^GCGCC
KpnI         1607  GGTACC               GGTAC^C
MfeI         1673  CAATTG               C^AATTG
MroI         2660  TCCGGA               T^CCGGA
MspA1I        647  CAGCTG               C[AC]G^C[GT]G
MunI         1673  CAATTG               C^AATTG
MvnI         1058  CGCG                 CG^CG
NarI         1330  GGCGCC               GG^CGCC
NcoI          417  CCATGG               C^CATGG
NsiI         1921  ATGCAT               ATGCA^T
PflMI         837  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1360  ACCGGT               A^CCGGT
PmaCI        2566  CACGTG               CAC^GTG
PmlI         2566  CACGTG               CAC^GTG
PshAI         558  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         647  CAGCTG               CAG^CTG
SapI         2269  GCTCTTC              GCTCTTC(1/4)
ScaI          191  AGTACT               AGT^ACT
SfoI         1330  GGCGCC               GGC^GCC
SgrAI        1359  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         2789  CCCGGG               CCC^GGG
SphI          732  GCATGC               GCATG^C
Van91I        837  CCACATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2225  CCAACATTCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2789  CCCGGG               C^CCGGG
XmaI         2789  CCCGGG               C^CCGGG
XmnI         2344  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AlwNI         755  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
              942  CAGCATCTG           
AseI          861  ATTAAT               AT^TAAT
             1000  ATTAAT              
AsnI          861  ATTAAT               AT^TAAT
             1000  ATTAAT              
AvaI         1703  CCCGAG               C^[CT]CG[GA]G
             2789  CCCGGG              
AviII         656  TGCGCA               TGC^GCA
             1820  TGCGCA              
BalI         1601  TGGCCA               TGG^CCA
             2549  TGGCCA              
BfaI         1085  CTAG                 C^TAG
             1186  CTAG                
BsaAI        2121  TACGTG               [CT]AC^GT[GA]
             2566  CACGTG              
BsaBI        1950  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2167  GATTGGGATC          
BsaI           85  GAGACC               GGTCTC(1/5)
              232  GAGACC              
BsaWI        1360  ACCGGT               [AT]^CCGG[AT]
             2660  TCCGGA              
BsmBI        2526  CGTCTC               CGTCTC(1/5)
             1990  GAGACG              
BsoBI        1703  CCCGAG               C^[CT]CG[GA]G
             2789  CCCGGG              
Bsp1407I     1442  TGTACA               T^GTACA
             2603  TGTACA              
BspHI         141  TCATGA               T^CATGA
             2798  TCATGA              
BsrFI         782  ACCGGC               [GA]^CCGG[CT]
             1360  ACCGGT              
BsrGI        1442  TGTACA               T^GTACA
             2603  TGTACA              
BstXI        1853  CCACCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2225  CCAACATTCTGG        
Cfr10I        782  ACCGGC               [GA]^CCGG[CT]
             1360  ACCGGT              
DraIII        759  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              838  CACATTGTG           
FauI         2810  CCCGC                CCCGC(4/6)
             2411  GCGGG               
FspI          656  TGCGCA               TGC^GCA
             1820  TGCGCA              
HaeII         476  AGCGCT               [GA]GCGC^[CT]
             1330  GGCGCC              
HgaI         1056  GACGC                GACGC(5/10)
             2525  GCGTC               
HincII       1783  GTCAAC               GT[CT]^[GA]AC
             1828  GTCAAC              
HindII       1783  GTCAAC               GT[CT]^[GA]AC
             1828  GTCAAC              
MaeI         1085  CTAG                 C^TAG
             1186  CTAG                
MamI         1950  GATAGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2167  GATTGGGATC          
MluNI        1601  TGGCCA               TGG^CCA
             2549  TGGCCA              
MscI         1601  TGGCCA               TGG^CCA
             2549  TGGCCA              
PshBI         861  ATTAAT               AT^TAAT
             1000  ATTAAT              
PstI         2516  CTGCAG               CTGCA^G
             2531  CTGCAG              
RcaI          141  TCATGA               T^CATGA
             2798  TCATGA              
SacI          379  GAGCTC               GAGCT^C
             1900  GAGCTC              
SfcI         2516  CTGCAG               C^T[GA][CT]AG
             2531  CTGCAG              
SmlI         2014  CTCAAG               C^T[CT][GA]AG
             2074  CTTGAG              
SspBI        1442  TGTACA               T^GTACA
             2603  TGTACA              
TfiI           52  GATTC                G^A[AT]TC
             2349  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BclI        BcnI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspLU11I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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