Restriction map for pF1KSDA0026
 (Circular) (Six-base) MAPSORT of: pF1KSDA0026.seq  Check: 2779  from: 1  to: 4001



pF1KSDA0026  4001 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:   1039    1039 
   Size:       4001
 
Acc65I G'GTAC_C

Cuts at:   1017    1017 
   Size:       4001
 
AclI AA'CG_TT

Cuts at:   3841    3841 
   Size:       4001
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    314     524    1826    2258    3005    3273     314 
   Size:        210    1302     432     747     268    1042

  Fragments arranged by size: 

                1302    1042     747     432     268     210
 
AflIII A'CryG_T

Cuts at:   1353    2457    3434    1353 
   Size:       1104     977    1920

  Fragments arranged by size: 

                1920    1104     977
 
AgeI A'CCGG_T

Cuts at:   2389    2389 
   Size:       4001
 
AhdI GACnn_n'nnGTC

Cuts at:    257     257 
   Size:       4001
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    733     733 
   Size:       4001
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    272     304     272 
   Size:         32    3969
 
AlwNI CAG_nnn'CTG

Cuts at:   1306    2873    1306 
   Size:       1567    2434
 
ApaI G_GGCC'C

Cuts at:    435     435 
   Size:       4001
 
ApaLI G'TGCA_C

Cuts at:   2771    2771 
   Size:       4001
 
ApoI r'AATT_y

Cuts at:    727     921    1005    3354    3610     727 
   Size:        194      84    2349     256    1118

  Fragments arranged by size: 

                2349    1118     256     194      84
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4001
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4001
 
AvaI C'yCGr_G

Cuts at:    434    1021     434 
   Size:        587    3414
 
BamHI G'GATC_C

Cuts at:    334    1026     334 
   Size:        692    3309
 
BanI G'GyrC_C

Cuts at:   1017    1431    1679    1714    1017 
   Size:        414     248      35    3304

  Fragments arranged by size: 

                3304     414     248      35
 
BanII G_rGCy'C

Cuts at:    435    1015    2045     435 
   Size:        580    1030    2391

  Fragments arranged by size: 

                2391    1030     580
 
BbeI G_GCGC'C

Cuts at:   1435    1683    1435 
   Size:        248    3753
 
BbsI GAAGACnn'nnnn_

Cuts at:    256     352     373     256 
   Size:         96      21    3884

  Fragments arranged by size: 

                3884      96      21
 
BbvCI CC'TCA_GC

Cuts at:    410     410 
   Size:       4001
 
BciVI GTATCCnnnnn_n'

Cuts at:   1899    2660    4001    1899 
   Size:        761    1341    1899

  Fragments arranged by size: 

                1899    1341     761
 
BclI T'GATC_A

Cuts at:   1521    1521 
   Size:       4001
 
BglII A'GATC_T

Cuts at:   1516    3553    1516 
   Size:       2037    1964
 
BlpI GC'TnA_GC

Cuts at:   1113    1113 
   Size:       4001
 
Bme1580I G_kGCm'C

Cuts at:    435     712    1626    1719    2775     435 
   Size:        277     914      93    1056    1661

  Fragments arranged by size: 

                1661    1056     914     277      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    543     735    1213    1294    1629    3773     543 
   Size:        192     478      81     335    2144     771

  Fragments arranged by size: 

                2144     771     478     335     192      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2368    2368 
   Size:       4001
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    622     622 
   Size:       4001
 
Bpu10I CC'TnA_GC

Cuts at:    410     410 
   Size:       4001
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1177    2056    2548    2846    1177 
   Size:        879     492     298    2332

  Fragments arranged by size: 

                2332     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3705    3705 
   Size:       4001
 
BsaAI yAC'GTr

Cuts at:    993    1354     993 
   Size:        361    3640
 
BsaBI GATnn'nnATC

Cuts at:      6    1515    3552       6 
   Size:       1509    2037     455

  Fragments arranged by size: 

                2037    1509     455
 
BsaHI Gr'CG_yC

Cuts at:   1432    1680    2382    3878    1432 
   Size:        248     702    1496    1555

  Fragments arranged by size: 

                1555    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1190    1711    2389    2663    2810    3221    1190 
   Size:        521     678     274     147     411    1970

  Fragments arranged by size: 

                1970     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    272     302     272 
   Size:         30    3971
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    228     475     619     228 
   Size:        247     144    3610

  Fragments arranged by size: 

                3610     247     144
 
BseYI C'CCAG_C

Cuts at:   1426    2184    2761    3257    1426 
   Size:        758     577     496    2170

  Fragments arranged by size: 

                2170     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1543    1589    2797     104 
   Size:       1439      46    1208    1308

  Fragments arranged by size: 

                1439    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    801    1015    1793    1983    2775     801 
   Size:        214     778     190     792    2027

  Fragments arranged by size: 

                2027     792     778     214     190
 
Bsp1286I G_dGCh'C

Cuts at:    435     712     801    1015    1626    1719    1793    1983    2045 
   Size:        277      89     214     611      93      74     190      62

Cuts at:   2045    2775     435 
   Size:        730    1661

  Fragments arranged by size: 

                1661     730     611     277     214     190      93      89
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3221    3221 
   Size:       4001
 
BspHI T'CATG_A

Cuts at:   3177    3177 
   Size:       4001
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1051    1265    1567    1948    1051 
   Size:        214     302     381    3104

  Fragments arranged by size: 

                3104     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2293    2347    3831    3996      50 
   Size:       2243      54    1484     165      55

  Fragments arranged by size: 

                2243    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    842    1913     842 
   Size:       1071    2930
 
BsrFI r'CCGG_y

Cuts at:   1999    2180    2389    1999 
   Size:        181     209    3611

  Fragments arranged by size: 

                3611     209     181
 
BssSI C'ACGA_G

Cuts at:    155    2272    2630     155 
   Size:       2117     358    1526

  Fragments arranged by size: 

                2117    1526     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1378    1378 
   Size:       4001
 
BstBI TT'CG_AA

Cuts at:    110    2362     110 
   Size:       2252    1749
 
BstXI CCAn_nnnn'nTGG

Cuts at:    299     793     299 
   Size:        494    3507
 
BstYI r'GATC_y

Cuts at:      1     334    1026    1493    1516    1851    2097    3109    3195 
   Size:        333     692     467      23     335     246    1012      86

Cuts at:   3195    3207    3553       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     692     467     449     346     335     333     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    240     240 
   Size:       4001
 
BtgI C'CryG_G

Cuts at:    517     517 
   Size:       4001
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1951    2132    2295    1951 
   Size:        181     163    3657

  Fragments arranged by size: 

                3657     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1244    1244 
   Size:       4001
 
DraI TTT'AAA

Cuts at:   1000    1000 
   Size:       4001
 
DrdI GACnn_nn'nnGTC

Cuts at:   1707    2565    1707 
   Size:        858    3143
 
EaeI y'GGCC_r

Cuts at:   1586    1760    2151    2178    3523    1586 
   Size:        174     391      27    1345    2064

  Fragments arranged by size: 

                2064    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1586    1586 
   Size:       4001
 
EarI CTCTTCn'nnn_

Cuts at:    173    2024    2234     173 
   Size:       1851     210    1940

  Fragments arranged by size: 

                1940    1851     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2209    2519    2665    3811    2209 
   Size:        310     146    1146    2399

  Fragments arranged by size: 

                2399    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1013    1013 
   Size:       4001
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    314     524     622    1826    2258    3005    3273     314 
   Size:        210      98    1204     432     747     268    1042

  Fragments arranged by size: 

                1204    1042     747     432     268     210      98
 
EcoO109I rG'GnC_Cy

Cuts at:    233     431     737    1140     233 
   Size:        198     306     403    3094

  Fragments arranged by size: 

                3094     403     306     198
 
EcoRI G'AATT_C

Cuts at:    921    1005     921 
   Size:         84    3917
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2020    2020 
   Size:       4001
 
FspI TGC'GCA

Cuts at:   1782    1782 
   Size:       4001
 
HaeII r_GCGC'y

Cuts at:   1435    1683    2705    3684    1435 
   Size:        248    1022     979    1752

  Fragments arranged by size: 

                1752    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    243     275    1843    1873    1875    1905     243 
   Size:         32    1568      30       2      30    2339

  Fragments arranged by size: 

                2339    1568      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1040    1040 
   Size:       4001
 
KasI G'GCGC_C

Cuts at:   1431    1679    1431 
   Size:        248    3753
 
KpnI G_GTAC'C

Cuts at:   1021    1021 
   Size:       4001
 
MluI A'CGCG_T

Cuts at:   3434    3434 
   Size:       4001
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2672    2856    2672 
   Size:        184    3817
 
MscI TGG'CCA

Cuts at:   1762    1762 
   Size:       4001
 
MslI CAynn'nnrTG

Cuts at:    770    1990    2117     770 
   Size:       1220     127    2654

  Fragments arranged by size: 

                2654    1220     127
 
MspA1I CmG'CkG

Cuts at:    256     561     717    1112    1426    1786    2799    3044     256 
   Size:        305     156     395     314     360    1013     245    1213

  Fragments arranged by size: 

                1213    1013     395     360     314     305     245     156
            
 
NaeI GCC'GGC

Cuts at:   2182    2182 
   Size:       4001
 
NarI GG'CG_CC

Cuts at:   1432    1680    1432 
   Size:        248    3753
 
NcoI C'CATG_G

Cuts at:    517     517 
   Size:       4001
 
NgoMIV G'CCGG_C

Cuts at:   2180    2180 
   Size:       4001
 
NspI r_CATG'y

Cuts at:    213    1054    1554    2461     213 
   Size:        841     500     907    1753

  Fragments arranged by size: 

                1753     907     841     500
 
PciI A'CATG_T

Cuts at:   2457    2457 
   Size:       4001
 
PfoI T'CCnGG_A

Cuts at:    372     372 
   Size:       4001
 
PmeI GTTT'AAAC

Cuts at:   1000    1000 
   Size:       4001
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    272     304    3140    3172     272 
   Size:         32    2836      32    1101

  Fragments arranged by size: 

                2836    1101      32      32
 
PspOMI G'GGCC_C

Cuts at:    431     431 
   Size:       4001
 
PstI C_TGCA'G

Cuts at:    174     318    1048    1733     174 
   Size:        144     730     685    2442

  Fragments arranged by size: 

                2442     730     685     144
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4001
 
PvuII CAG'CTG

Cuts at:    256     561     717    1426    1786     256 
   Size:        305     156     709     360    2471

  Fragments arranged by size: 

                2471     709     360     305     156
 
RsrII CG'GwC_CG

Cuts at:   2196    2196 
   Size:       4001
 
SacI G_AGCT'C

Cuts at:   1015    1015 
   Size:       4001
 
SalI G'TCGA_C

Cuts at:   1038    1038 
   Size:       4001
 
SapI GCTCTTCn'nnn_

Cuts at:   2024    2234    2024 
   Size:        210    3791
 
SbfI CC_TGCA'GG

Cuts at:   1048    1048 
   Size:       4001
 
ScaI AGT'ACT

Cuts at:   3518    3518 
   Size:       4001
 
SfcI C'TryA_G

Cuts at:     33     170     314    1044    1729    2722    2913      33 
   Size:        137     144     730     685     993     191    1121

  Fragments arranged by size: 

                1121     993     730     685     191     144     137
 
SfoI GGC'GCC

Cuts at:   1433    1681    1433 
   Size:        248    3753
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4001
 
SmaI CCC'GGG

Cuts at:    436    1023     436 
   Size:        587    3414
 
SmlI C'TyrA_G

Cuts at:   1156    2071    2563    2825    1156 
   Size:        915     492     262    2332

  Fragments arranged by size: 

                2332     915     492     262
 
SnaBI TAC'GTA

Cuts at:    993     993 
   Size:       4001
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4001
 
SphI G_CATG'C

Cuts at:   1054    1554    1054 
   Size:        500    3501
 
SrfI GCCC'GGGC

Cuts at:    436     436 
   Size:       4001
 
SspI AAT'ATT

Cuts at:    691     691 
   Size:       4001
 
StyI C'CwwG_G

Cuts at:    517    1135    1489     517 
   Size:        618     354    3029

  Fragments arranged by size: 

                3029     618     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1584    1717    2176    2385    1584 
   Size:        133     459     209    3200

  Fragments arranged by size: 

                3200     459     209     133
 
TatI w'GTAC_w

Cuts at:   3516    3516 
   Size:       4001
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3518    3550    3518 
   Size:         32    3969
 
Tth111I GACn'n_nGTC

Cuts at:   1798    1798 
   Size:       4001
 
XbaI T'CTAG_A

Cuts at:   1032    1032 
   Size:       4001
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1496    1496 
   Size:       4001
 
XmaI C'CCGG_G

Cuts at:    434    1021     434 
   Size:        587    3414
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI
     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AscI    AvrII     BaeI
     BcgI    BfrBI     BglI    BmgBI     BmtI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI     StuI     SwaI     XhoI     XmnI     ZraI
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