Restriction map for pF1KSDA0026
 (Linear) (Six-base) MAPSORT of: KIAA0026.seq  Check: 9390  from: 1  to: 864



KIAA0026.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     398     864 
   Size:        188     210     466

  Fragments arranged by size: 

                 466     210     188
 
AhdI GACnn_n'nnGTC

Cuts at:      0     131     864 
   Size:        131     733
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     607     864 
   Size:        607     257
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178     864 
   Size:        146      32     686

  Fragments arranged by size: 

                 686     146      32
 
ApaI G_GGCC'C

Cuts at:      0     309     864 
   Size:        309     555
 
ApoI r'AATT_y

Cuts at:      0     601     795     864 
   Size:        601     194      69

  Fragments arranged by size: 

                 601     194      69
 
AvaI C'yCGr_G

Cuts at:      0     308     864 
   Size:        308     556
 
BamHI G'GATC_C

Cuts at:      0     208     864 
   Size:        208     656
 
BanII G_rGCy'C

Cuts at:      0     309     864 
   Size:        309     555
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     130     226     247     864 
   Size:        130      96      21     617

  Fragments arranged by size: 

                 617     130      96      21
 
BbvCI CC'TCA_GC

Cuts at:      0     284     864 
   Size:        284     580
 
Bme1580I G_kGCm'C

Cuts at:      0     309     586     864 
   Size:        309     277     278

  Fragments arranged by size: 

                 309     278     277
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     417     609     864 
   Size:        417     192     255

  Fragments arranged by size: 

                 417     255     192
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     864 
   Size:        496     368
 
Bpu10I CC'TnA_GC

Cuts at:      0     284     864 
   Size:        284     580
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     146     176     864 
   Size:        146      30     688

  Fragments arranged by size: 

                 688     146      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     102     349     493     864 
   Size:        102     247     144     371

  Fragments arranged by size: 

                 371     247     144     102
 
BsiHKAI G_wGCw'C

Cuts at:      0     675     864 
   Size:        675     189
 
Bsp1286I G_dGCh'C

Cuts at:      0     309     586     675     864 
   Size:        309     277      89     189

  Fragments arranged by size: 

                 309     277     189      89
 
BsrDI GCAATG_nn'

Cuts at:      0     716     864 
   Size:        716     148
 
BssSI C'ACGA_G

Cuts at:      0      29     864 
   Size:         29     835
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     173     667     864 
   Size:        173     494     197

  Fragments arranged by size: 

                 494     197     173
 
BstYI r'GATC_y

Cuts at:      0     208     864 
   Size:        208     656
 
Bsu36I CC'TnA_GG

Cuts at:      0     114     864 
   Size:        114     750
 
BtgI C'CryG_G

Cuts at:      0     391     864 
   Size:        391     473
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     864 
   Size:         47     817
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     398     496     864 
   Size:        188     210      98     368

  Fragments arranged by size: 

                 368     210     188      98
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     305     611     864 
   Size:        107     198     306     253

  Fragments arranged by size: 

                 306     253     198     107
 
EcoRI G'AATT_C

Cuts at:      0     795     864 
   Size:        795      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     117     149     864 
   Size:        117      32     715

  Fragments arranged by size: 

                 715     117      32
 
MslI CAynn'nnrTG

Cuts at:      0     644     864 
   Size:        644     220
 
MspA1I CmG'CkG

Cuts at:      0     130     435     591     864 
   Size:        130     305     156     273

  Fragments arranged by size: 

                 305     273     156     130
 
NcoI C'CATG_G

Cuts at:      0     391     864 
   Size:        391     473
 
NspI r_CATG'y

Cuts at:      0      87     864 
   Size:         87     777
 
PfoI T'CCnGG_A

Cuts at:      0     246     864 
   Size:        246     618
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     146     178     864 
   Size:        146      32     686

  Fragments arranged by size: 

                 686     146      32
 
PspOMI G'GGCC_C

Cuts at:      0     305     864 
   Size:        305     559
 
PstI C_TGCA'G

Cuts at:      0      48     192     864 
   Size:         48     144     672

  Fragments arranged by size: 

                 672     144      48
 
PvuII CAG'CTG

Cuts at:      0     130     435     591     864 
   Size:        130     305     156     273

  Fragments arranged by size: 

                 305     273     156     130
 
SfcI C'TryA_G

Cuts at:      0      44     188     864 
   Size:         44     144     676

  Fragments arranged by size: 

                 676     144      44
 
SmaI CCC'GGG

Cuts at:      0     310     864 
   Size:        310     554
 
SrfI GCCC'GGGC

Cuts at:      0     310     864 
   Size:        310     554
 
SspI AAT'ATT

Cuts at:      0     565     864 
   Size:        565     299
 
StyI C'CwwG_G

Cuts at:      0     391     864 
   Size:        391     473
 
XmaI C'CCGG_G

Cuts at:      0     308     864 
   Size:        308     556
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI     AloI     ApaI     ApoI     AvaI    BamHI
    BanII     BbsI    BbvCI Bme1580I     BmrI     BpmI   Bpu10I    BsaXI
    BseRI  BsiHKAI Bsp1286I    BsrDI    BssSI    BstXI    BstYI   Bsu36I
     BtgI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I     MslI   MspA1I
     NcoI     NspI     PfoI     PpiI   PspOMI     PstI    PvuII     SfcI
     SmaI     SrfI     SspI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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