Restriction map for pF1KSDA0025
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BclI        BcnI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DraI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SalI        SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI          130  GTCTAC               GT^[AC][GT]AC
AcsI          726  AAATTT               [GA]^AATT[CT]
Alw44I        599  GTGCAC               G^TGCAC
AlwNI         560  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          349  CCTGAGG              CC^T[ACGT]AGG
ApaLI         599  GTGCAC               G^TGCAC
ApoI          726  AAATTT               [GA]^AATT[CT]
Asp700I       385  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI         1069  CTAG                 C^TAG
BsaWI         478  TCCGGT               [AT]^CCGG[AT]
BsgI          623  CTGCAC               GTGCAG(16/14)
BsiEI          84  CGACCG               CG[GA][CT]^CG
BsrDI         257  GCAATG               GCAATG(2/0)
BsrFI         939  GCCGGT               [GA]^CCGG[CT]
BsrI          651  CCAGT                ACTGG(1/-1)
Bsu36I        349  CCTGAGG              CC^T[ACGT]AGG
CfoI           57  GCGC                 GCG^C
Cfr10I        939  GCCGGT               [GA]^CCGG[CT]
DsaI           87  CCGCGG               C^C[GA][CT]GG
Eco81I        349  CCTGAGG              CC^T[ACGT]AGG
HaeII          56  AGCGCC               [GA]GCGC^[CT]
HhaI           57  GCGC                 GCG^C
HinP1I         57  GCGC                 G^CGC
KspI           87  CCGCGG               CCGC^GG
MaeI         1069  CTAG                 C^TAG
MslI          873  CATGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PvuII         659  CAGCTG               CAG^CTG
SacII          87  CCGCGG               CCGC^GG
SapI           42  GAAGAGC              GCTCTTC(1/4)
ScaI          539  AGTACT               AGT^ACT
SspI          551  AATATT               AAT^ATT
TaqI          783  TCGA                 T^CGA
Tsp45I         28  GTCAC                ^GT[GC]AC
TthHB8I       783  TCGA                 T^CGA
XmnI          385  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AlwI         1021  GATCC                GGATC(4/5)
              192  GGATC               
AspHI         599  GTGCAC               G[AT]GC[AT]^C
             1101  GAGCAC              
AvaI           19  CCCGAG               C^[CT]CG[GA]G
              136  CCCGAG              
BanII          22  GAGCCC               G[GA]GC[CT]^C
               45  GAGCCC              
BbsI         1033  GAAGAC               GAAGAC(2/6)
             1117  GTCTTC              
BciVI         345  GTATCC               GTATCC(6/5)
              836  GTATCC              
BglI          295  GCCAAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              442  GCCCAGCAGGC         
BpuAI        1033  GAAGAC               GAAGAC(2/6)
             1117  GTCTTC              
BsaI           10  GAGACC               GGTCTC(1/5)
              618  GGTCTC              
BsiHKAI       599  GTGCAC               G[AT]GC[AT]^C
             1101  GAGCAC              
BsmFI         209  GGGAC                GGGAC(10/14)
              339  GGGAC               
BsoBI          19  CCCGAG               C^[CT]CG[GA]G
              136  CCCGAG              
BspMI         627  ACCTGC               ACCTGC(4/8)
              902  ACCTGC              
BstAPI        237  GCATGTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              559  GCAGCCACTGC         
DpnII         193  GATC                 ^GATC
             1021  GATC                
DraIII        593  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              743  CACAAGGTG           
EcoT14I       296  CCAAGG               C^C[AT][AT]GG
              456  CCAAGG              
HapII         479  CCGG                 C^CGG
              940  CCGG                
HpaII         479  CCGG                 C^CGG
              940  CCGG                
HphI           39  GGTGA                GGTGA(8/7)
              195  TCACC               
MaeII         911  ACGT                 A^CGT
              977  ACGT                
MaeIII         28  GTCAC                ^GT[ACGT]AC
              482  GTTAC               
MboI          193  GATC                 ^GATC
             1021  GATC                
MspA1I         87  CCGCGG               C[AC]G^C[GT]G
              659  CAGCTG              
MspI          479  CCGG                 C^CGG
              940  CCGG                
NdeII         193  GATC                 ^GATC
             1021  GATC                
NspI          237  GCATGT               [GA]CATG^[CT]
              545  ACATGC              
PflMI         456  CCAAGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              955  CCAAATGATGG         
PleI            4  GAGTC                GAGTC(4/5)
              267  GAGTC               
PpuMI         465  GGGTCCT              [GA]G^G[AT]CC[CT]
              989  AGGACCC             
Sau3AI        193  GATC                 ^GATC
             1021  GATC                
StuI          430  AGGCCT               AGG^CCT
              459  AGGCCT              
StyI          296  CCAAGG               C^C[AT][AT]GG
              456  CCAAGG              
Tsp509I       168  AATT                 ^AATT
              727  AATT                
TspRI         564  CACTG                CA[GC]TG[ACGT][ACGT]^
             1017  CACTG               
Van91I        456  CCAAGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              955  CCAAATGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BbvI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmI        Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Cac8I       
Cfr13I      DdeI        DraII       EarI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HgaI        
HinfI       ItaI        Ksp632I     MboII       MnlI        
MseI        MvaI        MvnI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
SmlI        TfiI        Tru9I       TseI        
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