Restriction map for pF1KSDA0025
 (Linear) (Six-base) MAPSORT of: KIAA0025.seq  Check: 6876  from: 1  to: 1173



KIAA0025.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     636    1173 
   Size:        636     537
 
AccI GT'mk_AC

Cuts at:      0     131    1173 
   Size:        131    1042
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     487    1052    1173 
   Size:        455      32     565     121

  Fragments arranged by size: 

                 565     455     121      32
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     545    1173 
   Size:        545     628
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     388     420    1173 
   Size:        388      32     753

  Fragments arranged by size: 

                 753     388      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     565    1173 
   Size:        565     608
 
ApaLI G'TGCA_C

Cuts at:      0     599    1173 
   Size:        599     574
 
ApoI r'AATT_y

Cuts at:      0     726    1173 
   Size:        726     447
 
AvaI C'yCGr_G

Cuts at:      0      19     136    1173 
   Size:         19     117    1037

  Fragments arranged by size: 

                1037     117      19
 
BanII G_rGCy'C

Cuts at:      0      26      49    1173 
   Size:         26      23    1124

  Fragments arranged by size: 

                1124      26      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1040    1110    1173 
   Size:       1040      70      63

  Fragments arranged by size: 

                1040      70      63
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     356     847    1173 
   Size:        356     491     326

  Fragments arranged by size: 

                 491     356     326
 
BglI GCCn_nnn'nGGC

Cuts at:      0     301     448    1173 
   Size:        301     147     725

  Fragments arranged by size: 

                 725     301     147
 
Bme1580I G_kGCm'C

Cuts at:      0     603    1173 
   Size:        603     570
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     375    1173 
   Size:        375     798
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     617     734    1037    1173 
   Size:        114     503     117     303     136

  Fragments arranged by size: 

                 503     303     136     117     114
 
Bpu10I CC'TnA_GC

Cuts at:      0     121     859    1173 
   Size:        121     738     314

  Fragments arranged by size: 

                 738     314     121
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     345     411     680    1173 
   Size:         94     251      66     269     493

  Fragments arranged by size: 

                 493     269     251      94      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       4     624    1173 
   Size:          4     620     549

  Fragments arranged by size: 

                 620     549       4
 
BsaWI w'CCGG_w

Cuts at:      0     478    1173 
   Size:        478     695
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     835     865    1173 
   Size:        835      30     308

  Fragments arranged by size: 

                 835     308      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     331     683     843     959    1173 
   Size:        331     352     160     116     214

  Fragments arranged by size: 

                 352     331     214     160     116
 
BseYI C'CCAG_C

Cuts at:      0     443    1158    1173 
   Size:        443     715      15

  Fragments arranged by size: 

                 715     443      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608    1173 
   Size:        608     565
 
BsiEI CG_ry'CG

Cuts at:      0      87    1173 
   Size:         87    1086
 
BsiHKAI G_wGCw'C

Cuts at:      0     603    1105    1173 
   Size:        603     502      68

  Fragments arranged by size: 

                 603     502      68
 
BsmI GAATG_Cn'

Cuts at:      0     451     690     744    1173 
   Size:        451     239      54     429

  Fragments arranged by size: 

                 451     429     239      54
 
Bsp1286I G_dGCh'C

Cuts at:      0      26      49     603    1105    1173 
   Size:         26      23     554     502      68

  Fragments arranged by size: 

                 554     502      68      26      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     636     911    1173 
   Size:        636     275     262

  Fragments arranged by size: 

                 636     275     262
 
BsrDI GCAATG_nn'

Cuts at:      0     264    1173 
   Size:        264     909
 
BsrFI r'CCGG_y

Cuts at:      0     939    1173 
   Size:        939     234
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     243     565    1173 
   Size:        243     322     608

  Fragments arranged by size: 

                 608     322     243
 
Bsu36I CC'TnA_GG

Cuts at:      0     350    1173 
   Size:        350     823
 
BtgI C'CryG_G

Cuts at:      0      87    1173 
   Size:         87    1086
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1149    1173 
   Size:       1149      24
 
BtsI GCAGTG_nn'

Cuts at:      0     563    1173 
   Size:        563     610
 
DraIII CAC_nnn'GTG

Cuts at:      0     598     748    1173 
   Size:        598     150     425

  Fragments arranged by size: 

                 598     425     150
 
EarI CTCTTCn'nnn_

Cuts at:      0      37     259    1145    1173 
   Size:         37     222     886      28

  Fragments arranged by size: 

                 886     222      37      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     455     487     617     734    1037    1052    1173 
   Size:        114     341      32     130     117     303      15     121

  Fragments arranged by size: 

                 341     303     130     121     117     114      32      15
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     466     990    1153    1173 
   Size:        466     524     163      20

  Fragments arranged by size: 

                 524     466     163      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1146    1173 
   Size:       1146      27
 
HaeII r_GCGC'y

Cuts at:      0      60    1173 
   Size:         60    1113
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     773    1173 
   Size:        170     603     400

  Fragments arranged by size: 

                 603     400     170
 
MslI CAynn'nnrTG

Cuts at:      0     877    1173 
   Size:        877     296
 
MspA1I CmG'CkG

Cuts at:      0      89     661    1173 
   Size:         89     572     512

  Fragments arranged by size: 

                 572     512      89
 
NspI r_CATG'y

Cuts at:      0     241     549    1173 
   Size:        241     308     624

  Fragments arranged by size: 

                 624     308     241
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     462     961    1173 
   Size:        462     499     212

  Fragments arranged by size: 

                 499     462     212
 
PfoI T'CCnGG_A

Cuts at:      0     711    1173 
   Size:        711     462
 
PpuMI rG'GwC_Cy

Cuts at:      0     466     990    1173 
   Size:        466     524     183

  Fragments arranged by size: 

                 524     466     183
 
PvuII CAG'CTG

Cuts at:      0     661    1173 
   Size:        661     512
 
SacII CC_GC'GG

Cuts at:      0      90    1173 
   Size:         90    1083
 
SapI GCTCTTCn'nnn_

Cuts at:      0      37    1173 
   Size:         37    1136
 
ScaI AGT'ACT

Cuts at:      0     541    1173 
   Size:        541     632
 
SmlI C'TyrA_G

Cuts at:      0     109     360     426     695    1173 
   Size:        109     251      66     269     478

  Fragments arranged by size: 

                 478     269     251     109      66
 
SspI AAT'ATT

Cuts at:      0     553    1173 
   Size:        553     620
 
StuI AGG'CCT

Cuts at:      0     432     461    1173 
   Size:        432      29     712

  Fragments arranged by size: 

                 712     432      29
 
StyI C'CwwG_G

Cuts at:      0     296     456    1173 
   Size:        296     160     717

  Fragments arranged by size: 

                 717     296     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28     485     905     948    1173 
   Size:         28     457     420      43     225

  Fragments arranged by size: 

                 457     420     225      43      28
 
TatI w'GTAC_w

Cuts at:      0     539    1065    1173 
   Size:        539     526     108

  Fragments arranged by size: 

                 539     526     108
 
XmnI GAAnn'nnTTC

Cuts at:      0     389    1173 
   Size:        389     784
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AvaI    BanII     BbsI    BciVI     BglI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EarI  Eco57MI EcoO109I
     FalI    HaeII     MmeI     MslI   MspA1I     NspI    PflMI     PfoI
    PpuMI    PvuII    SacII     SapI     ScaI     SmlI     SspI     StuI
     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaHI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI    SanDI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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