Restriction map for pF1KSDA0022
 (Circular) (Six-base) MAPSORT of: pF1KSDA0022.seq  Check: 7146  from: 1  to: 3835



pF1KSDA0022  3835 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    871     871 
   Size:       3835
 
Acc65I G'GTAC_C

Cuts at:    849     849 
   Size:       3835
 
AclI AA'CG_TT

Cuts at:   3675    3675 
   Size:       3835
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1658    2090    2839    3107    1658 
   Size:        432     749     268    2386

  Fragments arranged by size: 

                2386     749     432     268
 
AflIII A'CryG_T

Cuts at:   1185    2291    3268    1185 
   Size:       1106     977    1752

  Fragments arranged by size: 

                1752    1106     977
 
AgeI A'CCGG_T

Cuts at:   2223    2223 
   Size:       3835
 
AlwNI CAG_nnn'CTG

Cuts at:   1138    2707    1138 
   Size:       1569    2266
 
ApaLI G'TGCA_C

Cuts at:   2605    2605 
   Size:       3835
 
ApoI r'AATT_y

Cuts at:    837    3188    3444     837 
   Size:       2351     256    1228

  Fragments arranged by size: 

                2351    1228     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3835
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3835
 
AvaI C'yCGr_G

Cuts at:    853     853 
   Size:       3835
 
BamHI G'GATC_C

Cuts at:    858     858 
   Size:       3835
 
BanI G'GyrC_C

Cuts at:    849    1263    1511    1546     849 
   Size:        414     248      35    3138

  Fragments arranged by size: 

                3138     414     248      35
 
BanII G_rGCy'C

Cuts at:    847    1877     847 
   Size:       1030    2805
 
BbeI G_GCGC'C

Cuts at:   1267    1515    1267 
   Size:        248    3587
 
BbsI GAAGACnn'nnnn_

Cuts at:    776     776 
   Size:       3835
 
BciVI GTATCCnnnnn_n'

Cuts at:    348    1731    2494    3835     348 
   Size:       1383     763    1341     348

  Fragments arranged by size: 

                1383    1341     763     348
 
BclI T'GATC_A

Cuts at:   1353    1353 
   Size:       3835
 
BglII A'GATC_T

Cuts at:   1348    3387    1348 
   Size:       2039    1796
 
BlpI GC'TnA_GC

Cuts at:    945     945 
   Size:       3835
 
Bme1580I G_kGCm'C

Cuts at:   1458    1551    2609    1458 
   Size:         93    1058    2684

  Fragments arranged by size: 

                2684    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1045    1126    1461    3607    1045 
   Size:         81     335    2146    1273

  Fragments arranged by size: 

                2146    1273     335      81
 
BmtI G_CTAG'C

Cuts at:    659     659 
   Size:       3835
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2200    2200 
   Size:       3835
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1009    1888    2382    2680    1009 
   Size:        879     494     298    2164

  Fragments arranged by size: 

                2164     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3539    3539 
   Size:       3835
 
BsaAI yAC'GTr

Cuts at:    825    1186     825 
   Size:        361    3474
 
BsaBI GATnn'nnATC

Cuts at:      6    1347    3386       6 
   Size:       1341    2039     455

  Fragments arranged by size: 

                2039    1341     455
 
BsaHI Gr'CG_yC

Cuts at:   1264    1512    2216    3712    1264 
   Size:        248     704    1496    1387

  Fragments arranged by size: 

                1496    1387     704     248
 
BsaWI w'CCGG_w

Cuts at:   1022    1543    2223    2497    2644    3055    1022 
   Size:        521     680     274     147     411    1802

  Fragments arranged by size: 

                1802     680     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    165    1258    2016    2595    3091     165 
   Size:       1093     758     579     496     909

  Fragments arranged by size: 

                1093     909     758     579     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    502     502 
   Size:       3835
 
BsiEI CG_ry'CG

Cuts at:    104    1375    1421    2631     104 
   Size:       1271      46    1210    1308

  Fragments arranged by size: 

                1308    1271    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:    847    1625    1815    2609     847 
   Size:        778     190     794    2073

  Fragments arranged by size: 

                2073     794     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    847    1458    1551    1625    1815    1877    2609     847 
   Size:        611      93      74     190      62     732    2073

  Fragments arranged by size: 

                2073     732     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3055    3055 
   Size:       3835
 
BspHI T'CATG_A

Cuts at:   3011    3011 
   Size:       3835
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    883    1097    1399    1780     883 
   Size:        214     302     381    2938

  Fragments arranged by size: 

                2938     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     307    2125    2179    3665    3830      50 
   Size:        257    1818      54    1486     165      55

  Fragments arranged by size: 

                1818    1486     257     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    376    1745     376 
   Size:       1369    2466
 
BsrFI r'CCGG_y

Cuts at:   1831    2012    2223    1831 
   Size:        181     211    3443

  Fragments arranged by size: 

                3443     211     181
 
BsrGI T'GTAC_A

Cuts at:    701     701 
   Size:       3835
 
BssSI C'ACGA_G

Cuts at:   2104    2464    2104 
   Size:        360    3475
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1210    1210 
   Size:       3835
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       3835
 
BstYI r'GATC_y

Cuts at:      1     858    1325    1348    1683    1929    2943    3029    3041 
   Size:        857     467      23     335     246    1014      86      12

Cuts at:   3041    3387       1 
   Size:        346     449

  Fragments arranged by size: 

                1014     857     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    300     300 
   Size:       3835
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1783    1964    2127    1783 
   Size:        181     163    3491

  Fragments arranged by size: 

                3491     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1076    1076 
   Size:       3835
 
DraI TTT'AAA

Cuts at:    832     832 
   Size:       3835
 
DrdI GACnn_nn'nnGTC

Cuts at:   1539    2399    1539 
   Size:        860    2975
 
EaeI y'GGCC_r

Cuts at:   1418    1592    1983    2010    3357    1418 
   Size:        174     391      27    1347    1896

  Fragments arranged by size: 

                1896    1347     391     174      27
 
EagI C'GGCC_G

Cuts at:   1418    1418 
   Size:       3835
 
EarI CTCTTCn'nnn_

Cuts at:   1856    2066    1856 
   Size:        210    3625
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2041    2353    2499    3645    2041 
   Size:        312     146    1146    2231

  Fragments arranged by size: 

                2231    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:    845     845 
   Size:       3835
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1658    2090    2839    3107    1658 
   Size:        432     749     268    2386

  Fragments arranged by size: 

                2386     749     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    972     972 
   Size:       3835
 
EcoRI G'AATT_C

Cuts at:    837     837 
   Size:       3835
 
EcoRV GAT'ATC

Cuts at:    390     390 
   Size:       3835
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1852    1852 
   Size:       3835
 
FspI TGC'GCA

Cuts at:   1614    1614 
   Size:       3835
 
HaeII r_GCGC'y

Cuts at:   1267    1515    2539    3518    1267 
   Size:        248    1024     979    1584

  Fragments arranged by size: 

                1584    1024     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1675    1705    1707    1737    1675 
   Size:         30       2      30    3773

  Fragments arranged by size: 

                3773      30      30       2
 
HincII GTy'rAC

Cuts at:    501     872     501 
   Size:        371    3464
 
KasI G'GCGC_C

Cuts at:   1263    1511    1263 
   Size:        248    3587
 
KpnI G_GTAC'C

Cuts at:    853     853 
   Size:       3835
 
MluI A'CGCG_T

Cuts at:   3268    3268 
   Size:       3835
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    768    2506    2690     768 
   Size:       1738     184    1913

  Fragments arranged by size: 

                1913    1738     184
 
MscI TGG'CCA

Cuts at:   1594    1594 
   Size:       3835
 
MslI CAynn'nnrTG

Cuts at:   1822    1949    1822 
   Size:        127    3708
 
MspA1I CmG'CkG

Cuts at:    178     944    1258    1618    2633    2878     178 
   Size:        766     314     360    1015     245    1135

  Fragments arranged by size: 

                1135    1015     766     360     314     245
 
NaeI GCC'GGC

Cuts at:   2014    2014 
   Size:       3835
 
NarI GG'CG_CC

Cuts at:   1264    1512    1264 
   Size:        248    3587
 
NcoI C'CATG_G

Cuts at:    300     300 
   Size:       3835
 
NgoMIV G'CCGG_C

Cuts at:   2012    2012 
   Size:       3835
 
NheI G'CTAG_C

Cuts at:    655     655 
   Size:       3835
 
NruI TCG'CGA

Cuts at:   2196    2196 
   Size:       3835
 
NspI r_CATG'y

Cuts at:    405     886    1386    2295     405 
   Size:        481     500     909    1945

  Fragments arranged by size: 

                1945     909     500     481
 
PciI A'CATG_T

Cuts at:   2291    2291 
   Size:       3835
 
PmeI GTTT'AAAC

Cuts at:    832     832 
   Size:       3835
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2974    3006    2974 
   Size:         32    3803
 
PsiI TTA'TAA

Cuts at:    764     764 
   Size:       3835
 
PstI C_TGCA'G

Cuts at:    880    1565     880 
   Size:        685    3150
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3835
 
PvuII CAG'CTG

Cuts at:   1258    1618    1258 
   Size:        360    3475
 
RsrII CG'GwC_CG

Cuts at:   2028    2028 
   Size:       3835
 
SacI G_AGCT'C

Cuts at:    847     847 
   Size:       3835
 
SalI G'TCGA_C

Cuts at:    870     870 
   Size:       3835
 
SapI GCTCTTCn'nnn_

Cuts at:   1856    2066    1856 
   Size:        210    3625
 
SbfI CC_TGCA'GG

Cuts at:    880     880 
   Size:       3835
 
ScaI AGT'ACT

Cuts at:   3352    3352 
   Size:       3835
 
SfcI C'TryA_G

Cuts at:     33     876    1561    2556    2747      33 
   Size:        843     685     995     191    1121

  Fragments arranged by size: 

                1121     995     843     685     191
 
SfoI GGC'GCC

Cuts at:   1265    1513    1265 
   Size:        248    3587
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3835
 
SmaI CCC'GGG

Cuts at:    855     855 
   Size:       3835
 
SmlI C'TyrA_G

Cuts at:    988    1903    2397    2659     988 
   Size:        915     494     262    2164

  Fragments arranged by size: 

                2164     915     494     262
 
SnaBI TAC'GTA

Cuts at:    825     825 
   Size:       3835
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3835
 
SphI G_CATG'C

Cuts at:    886    1386     886 
   Size:        500    3335
 
SspI AAT'ATT

Cuts at:    613     613 
   Size:       3835
 
StyI C'CwwG_G

Cuts at:    300     967    1321     300 
   Size:        667     354    2814

  Fragments arranged by size: 

                2814     667     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1416    1549    2008    2219    1416 
   Size:        133     459     211    3032

  Fragments arranged by size: 

                3032     459     211     133
 
TatI w'GTAC_w

Cuts at:    292     701    3350     292 
   Size:        409    2649     777

  Fragments arranged by size: 

                2649     777     409
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3352    3384    3352 
   Size:         32    3803
 
Tth111I GACn'n_nGTC

Cuts at:   1630    1630 
   Size:       3835
 
XbaI T'CTAG_A

Cuts at:    864     864 
   Size:       3835
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1328    1328 
   Size:       3835
 
XmaI C'CCGG_G

Cuts at:    853     853 
   Size:       3835
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NruI     NspI
     PciI     PmeI     PpiI     PsiI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BpmI   Bpu10I    BsaXI    BseRI    BsiWI     BsmI    BsmBI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NotI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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