Restriction map for pF1KSDA0020
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmFI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HapII       HgaI        HincII      
HindII      HpaI        HpaII       KpnI        KspI        
MamI        MfeI        MluI        MroI        MspA1I      
MspI        MunI        NaeI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SspBI       StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          646  GTCTAC               GT^[AC][GT]AC
AccII         634  CGCG                 CG^CG
AcyI          670  GGCGCC               G[GA]^CG[CT]C
BbeI          670  GGCGCC               GGCGC^C
BbrPI         301  CACGTG               CAC^GTG
BbsI          741  GAAGAC               GAAGAC(2/6)
BpuAI         741  GAAGAC               GAAGAC(2/6)
BsaAI         301  CACGTG               [CT]AC^GT[GA]
BsaHI         670  GGCGCC               G[GA]^CG[CT]C
BseRI        1396  CTCCTC               GAGGAG(10/8)
BsmAI        1139  GAGAC                GTCTC(1/5)
BsmBI        1139  GAGACG               CGTCTC(1/5)
BsmI         1158  GAATGC               GAATGC(1/-1)
BspMI        1058  ACCTGC               ACCTGC(4/8)
BssSI         119  CTCGTG               CACGAG(-5/-1)
BstUI         634  CGCG                 CG^CG
CfoI          671  GCGC                 GCG^C
DraI          292  TTTAAA               TTT^AAA
DraII         899  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          397  GAAGAG               CTCTTC(1/4)
EcoO109I      899  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       710  CCAAGG               C^C[AT][AT]GG
HaeII         670  GGCGCC               [GA]GCGC^[CT]
HhaI          671  GCGC                 GCG^C
Hin1I         670  GGCGCC               G[GA]^CG[CT]C
HinP1I        671  GCGC                 G^CGC
HphI          441  TCACC                GGTGA(8/7)
KasI          670  GGCGCC               G^GCGCC
Ksp632I       397  GAAGAG               CTCTTC(1/4)
MslI         1192  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          634  CGCG                 CG^CG
NarI          670  GGCGCC               GG^CGCC
PflMI        1074  CCAAGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         301  CACGTG               CAC^GTG
PmlI          301  CACGTG               CAC^GTG
PpuMI         899  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI         1235  CTGCAG               CTGCA^G
SapI          397  GAAGAGC              GCTCTTC(1/4)
SbfI         1234  CCTGCAGG             CCTGCA^GG
SfcI         1235  CTGCAG               C^T[GA][CT]AG
SfoI          670  GGCGCC               GGC^GCC
SphI          321  GCATGC               GCATG^C
Sse8387I     1234  CCTGCAGG             CCTGCA^GG
StyI          710  CCAAGG               C^C[AT][AT]GG
Van91I       1074  CCAAGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1163  CCATCGCCAGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          241  AAATTT               [GA]^AATT[CT]
             1499  AAATTC              
ApoI          241  AAATTT               [GA]^AATT[CT]
             1499  AAATTC              
AvaII         900  GGTCC                G^G[AT]CC
             1008  GGTCC               
BalI          680  TGGCCA               TGG^CCA
              768  TGGCCA              
BanI          670  GGCGCC               G^G[CT][GA]CC
             1375  GGTGCC              
BanII         400  GAGCTC               G[GA]GC[CT]^C
             1021  GAGCTC              
BglI          673  GCCAGAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1168  GCCAGCTTGGC         
BsaJI         710  CCAAGG               C^C[ACGT][ACGT]GG
             1355  CCTGGG              
BsgI          932  CTGCAC               GTGCAG(16/14)
             1439  CTGCAC              
BsrDI         863  CATTGC               GCAATG(2/0)
             1221  CATTGC              
BstYI         927  AGATCC               [GA]^GATC[CT]
             1126  GGATCT              
EaeI          680  TGGCCA               [CT]^GGCC[GA]
              768  TGGCCA              
HindIII       823  AAGCTT               A^AGCTT
             1449  AAGCTT              
MaeII         302  ACGT                 A^CGT
             1142  ACGT                
MflI          927  AGATCC               [GA]^GATC[CT]
             1126  GGATCT              
MluNI         680  TGGCCA               TGG^CCA
              768  TGGCCA              
MscI          680  TGGCCA               TGG^CCA
              768  TGGCCA              
NlaIV         670  GGCGCC               GG[ACGT]^[ACGT]CC
             1375  GGTGCC              
SacI          400  GAGCTC               GAGCT^C
             1021  GAGCTC              
SspI          218  AATATT               AAT^ATT
              229  AATATT              
TaqI          223  TCGA                 T^CGA
             1371  TCGA                
Tsp45I         29  GTGAC                ^GT[GC]AC
             1409  GTGAC               
TthHB8I       223  TCGA                 T^CGA
             1371  TCGA                
XhoII         927  AGATCC               [GA]^GATC[CT]
             1126  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
BbvI        BfaI        BmyI        BpmI        BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NspI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        Tru9I       
TseI        Tsp509I     TspRI       
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