Restriction map for pF1KSDA0020
 (Circular) (Six-base) MAPSORT of: pF1KSDA0020.seq  Check: 9485  from: 1  to: 4661



pF1KSDA0020  4661 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    773    1699     773 
   Size:        926    3735
 
Acc65I G'GTAC_C

Cuts at:   1677    1677 
   Size:       4661
 
AclI AA'CG_TT

Cuts at:   4501    4501 
   Size:       4661
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1399    1495    2486    2918    3665    3933    1399 
   Size:         96     991     432     747     268    2127

  Fragments arranged by size: 

                2127     991     747     432     268      96
 
AflIII A'CryG_T

Cuts at:   2013    3117    4094    2013 
   Size:       1104     977    2580

  Fragments arranged by size: 

                2580    1104     977
 
AgeI A'CCGG_T

Cuts at:   3049    3049 
   Size:       4661
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1358    1390    1462    1494    1358 
   Size:         32      72      32    4525

  Fragments arranged by size: 

                4525      72      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1966    3533    1966 
   Size:       1567    3094
 
ApaLI G'TGCA_C

Cuts at:   3431    3431 
   Size:       4661
 
ApoI r'AATT_y

Cuts at:    367    1625    1665    4014    4270     367 
   Size:       1258      40    2349     256     758

  Fragments arranged by size: 

                2349    1258     758     256      40
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4661
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4661
 
AvaI C'yCGr_G

Cuts at:   1681    1681 
   Size:       4661
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1118    1151    1118 
   Size:         33    4628
 
BamHI G'GATC_C

Cuts at:   1686    1686 
   Size:       4661
 
BanI G'GyrC_C

Cuts at:    796    1501    1677    2091    2339    2374     796 
   Size:        705     176     414     248      35    3083

  Fragments arranged by size: 

                3083     705     414     248     176      35
 
BanII G_rGCy'C

Cuts at:    530    1151    1675    2705     530 
   Size:        621     524    1030    2486

  Fragments arranged by size: 

                2486    1030     621     524
 
BbeI G_GCGC'C

Cuts at:    800    2095    2343     800 
   Size:       1295     248    3118

  Fragments arranged by size: 

                3118    1295     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    874     874 
   Size:       4661
 
BciVI GTATCCnnnnn_n'

Cuts at:   2559    3320    4661    2559 
   Size:        761    1341    2559

  Fragments arranged by size: 

                2559    1341     761
 
BclI T'GATC_A

Cuts at:   2181    2181 
   Size:       4661
 
BglI GCCn_nnn'nGGC

Cuts at:    805    1300     805 
   Size:        495    4166
 
BglII A'GATC_T

Cuts at:   2176    4213    2176 
   Size:       2037    2624
 
BlpI GC'TnA_GC

Cuts at:   1773    1773 
   Size:       4661
 
Bme1580I G_kGCm'C

Cuts at:   2286    2379    3435    2286 
   Size:         93    1056    3512

  Fragments arranged by size: 

                3512    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1873    1954    2289    4433    1873 
   Size:         81     335    2144    2101

  Fragments arranged by size: 

                2144    2101     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3028    3028 
   Size:       4661
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1151    1283    1510    1151 
   Size:        132     227    4302

  Fragments arranged by size: 

                4302     227     132
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1837    2716    3208    3506    1837 
   Size:        879     492     298    2992

  Fragments arranged by size: 

                2992     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4365    4365 
   Size:       4661
 
BsaAI yAC'GTr

Cuts at:    429    1653    2014     429 
   Size:       1224     361    3076

  Fragments arranged by size: 

                3076    1224     361
 
BsaBI GATnn'nnATC

Cuts at:      6    2175    4212       6 
   Size:       2169    2037     455

  Fragments arranged by size: 

                2169    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    797    2092    2340    3042    4538     797 
   Size:       1295     248     702    1496     920

  Fragments arranged by size: 

                1496    1295     920     702     248
 
BsaWI w'CCGG_w

Cuts at:   1850    2371    3049    3323    3470    3881    1850 
   Size:        521     678     274     147     411    2630

  Fragments arranged by size: 

                2630     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1133    1163    1413    1443    1133 
   Size:         30     250      30    4351

  Fragments arranged by size: 

                4351     250      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1513    1513 
   Size:       4661
 
BseYI C'CCAG_C

Cuts at:   2086    2844    3421    3917    2086 
   Size:        758     577     496    2830

  Fragments arranged by size: 

                2830     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1043    1550    1043 
   Size:        507    4154
 
BsiEI CG_ry'CG

Cuts at:    104    2203    2249    3457     104 
   Size:       2099      46    1208    1308

  Fragments arranged by size: 

                2099    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    530    1151    1648    1675    2453    2643    3435     530 
   Size:        621     497      27     778     190     792    1756

  Fragments arranged by size: 

                1756     792     778     621     497     190      27
 
BsmI GAATG_Cn'

Cuts at:   1290    1290 
   Size:       4661
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1259    1259 
   Size:       4661
 
Bsp1286I G_dGCh'C

Cuts at:    530    1151    1648    1675    2286    2379    2453    2643    2705 
   Size:        621     497      27     611      93      74     190      62

Cuts at:   2705    3435     530 
   Size:        730    1756

  Fragments arranged by size: 

                1756     730     621     611     497     190      93      74
                  62      27
 
BspEI T'CCGG_A

Cuts at:   3881    3881 
   Size:       4661
 
BspHI T'CATG_A

Cuts at:   3837    3837 
   Size:       4661
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1193    1711    1925    2227    2608    1193 
   Size:        518     214     302     381    3246

  Fragments arranged by size: 

                3246     518     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2953    3007    4491    4656      50 
   Size:       2903      54    1484     165      55

  Fragments arranged by size: 

                2903    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    988    1346    2573     988 
   Size:        358    1227    3076

  Fragments arranged by size: 

                3076    1227     358
 
BsrFI r'CCGG_y

Cuts at:   2659    2840    3049    2659 
   Size:        181     209    4271

  Fragments arranged by size: 

                4271     209     181
 
BssSI C'ACGA_G

Cuts at:    245    2932    3290     245 
   Size:       2687     358    1616

  Fragments arranged by size: 

                2687    1616     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2038    2038 
   Size:       4661
 
BstBI TT'CG_AA

Cuts at:    110    3022     110 
   Size:       2912    1749
 
BstYI r'GATC_y

Cuts at:      1    1053    1252    1686    2153    2176    2511    2757    3769 
   Size:       1052     199     434     467      23     335     246    1012

Cuts at:   3769    3855    3867    4213       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1052    1012     467     449     434     346     335     246
                 199      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1275    2611    2792    2955    1275 
   Size:       1336     181     163    2981

  Fragments arranged by size: 

                2981    1336     181     163
 
BtsI GCAGTG_nn'

Cuts at:    813    1904     813 
   Size:       1091    3570
 
DraI TTT'AAA

Cuts at:    420    1660     420 
   Size:       1240    3421
 
DrdI GACnn_nn'nnGTC

Cuts at:   2367    3225    2367 
   Size:        858    3803
 
EaeI y'GGCC_r

Cuts at:    806     894    2246    2420    2811    2838    4183     806 
   Size:         88    1352     174     391      27    1345    1284

  Fragments arranged by size: 

                1352    1345    1284     391     174      88      27
 
EagI C'GGCC_G

Cuts at:   2246    2246 
   Size:       4661
 
EarI CTCTTCn'nnn_

Cuts at:    518    2684    2894     518 
   Size:       2166     210    2285

  Fragments arranged by size: 

                2285    2166     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2869    3179    3325    4471    2869 
   Size:        310     146    1146    3059

  Fragments arranged by size: 

                3059    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    528    1149    1673     528 
   Size:        621     524    3516

  Fragments arranged by size: 

                3516     621     524
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1151    1283    1399    1495    1510    2486    2918    3665    3933 
   Size:        132     116      96      15     976     432     747     268

Cuts at:   3933    1151 
   Size:       1879

  Fragments arranged by size: 

                1879     976     747     432     268     132     116      96
                  15
 
EcoO109I rG'GnC_Cy

Cuts at:   1026    1800    1026 
   Size:        774    3887
 
EcoRI G'AATT_C

Cuts at:   1665    1665 
   Size:       4661
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2680    2680 
   Size:       4661
 
FspI TGC'GCA

Cuts at:   2442    2442 
   Size:       4661
 
HaeII r_GCGC'y

Cuts at:    800    2095    2343    3365    4344     800 
   Size:       1295     248    1022     979    1117

  Fragments arranged by size: 

                1295    1117    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    630     662    1133    1165    2503    2533    2535    2565     630 
   Size:         32     471      32    1338      30       2      30    2726

  Fragments arranged by size: 

                2726    1338     471      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1700    1700 
   Size:       4661
 
HindIII A'AGCT_T

Cuts at:    949    1575     949 
   Size:        626    4035
 
KasI G'GCGC_C

Cuts at:    796    2091    2339     796 
   Size:       1295     248    3118

  Fragments arranged by size: 

                3118    1295     248
 
KpnI G_GTAC'C

Cuts at:   1681    1681 
   Size:       4661
 
MluI A'CGCG_T

Cuts at:   4094    4094 
   Size:       4661
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3332    3516    3332 
   Size:        184    4477
 
MscI TGG'CCA

Cuts at:    808     896    2422     808 
   Size:         88    1526    3047

  Fragments arranged by size: 

                3047    1526      88
 
MslI CAynn'nnrTG

Cuts at:   1322    2650    2777    1322 
   Size:       1328     127    3206

  Fragments arranged by size: 

                3206    1328     127
 
MspA1I CmG'CkG

Cuts at:   1772    2086    2446    3459    3704    1772 
   Size:        314     360    1013     245    2729

  Fragments arranged by size: 

                2729    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2842    2842 
   Size:       4661
 
NarI GG'CG_CC

Cuts at:    797    2092    2340     797 
   Size:       1295     248    3118

  Fragments arranged by size: 

                3118    1295     248
 
NgoMIV G'CCGG_C

Cuts at:   2840    2840 
   Size:       4661
 
NspI r_CATG'y

Cuts at:    451     516    1459    1714    2214    3121     451 
   Size:         65     943     255     500     907    1991

  Fragments arranged by size: 

                1991     943     907     500     255      65
 
PciI A'CATG_T

Cuts at:   3117    3117 
   Size:       4661
 
PflMI CCAn_nnn'nTGG

Cuts at:   1206    1206 
   Size:       4661
 
PmeI GTTT'AAAC

Cuts at:   1660    1660 
   Size:       4661
 
PmlI CAC'GTG

Cuts at:    429     429 
   Size:       4661
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3800    3832    3800 
   Size:         32    4629
 
PpuMI rG'GwC_Cy

Cuts at:   1026    1026 
   Size:       4661
 
PstI C_TGCA'G

Cuts at:   1365    1708    2393    1365 
   Size:        343     685    3633

  Fragments arranged by size: 

                3633     685     343
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4661
 
PvuII CAG'CTG

Cuts at:   2086    2446    2086 
   Size:        360    4301
 
RsrII CG'GwC_CG

Cuts at:   2856    2856 
   Size:       4661
 
SacI G_AGCT'C

Cuts at:    530    1151    1675     530 
   Size:        621     524    3516

  Fragments arranged by size: 

                3516     621     524
 
SalI G'TCGA_C

Cuts at:   1698    1698 
   Size:       4661
 
SapI GCTCTTCn'nnn_

Cuts at:    518    2684    2894     518 
   Size:       2166     210    2285

  Fragments arranged by size: 

                2285    2166     210
 
SbfI CC_TGCA'GG

Cuts at:   1365    1708    1365 
   Size:        343    4318
 
ScaI AGT'ACT

Cuts at:   4178    4178 
   Size:       4661
 
SfcI C'TryA_G

Cuts at:     33    1361    1704    2389    3382    3573      33 
   Size:       1328     343     685     993     191    1121

  Fragments arranged by size: 

                1328    1121     993     685     343     191
 
SfoI GGC'GCC

Cuts at:    798    2093    2341     798 
   Size:       1295     248    3118

  Fragments arranged by size: 

                3118    1295     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4661
 
SmaI CCC'GGG

Cuts at:   1683    1683 
   Size:       4661
 
SmlI C'TyrA_G

Cuts at:   1816    2731    3223    3485    1816 
   Size:        915     492     262    2992

  Fragments arranged by size: 

                2992     915     492     262
 
SnaBI TAC'GTA

Cuts at:   1653    1653 
   Size:       4661
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4661
 
SphI G_CATG'C

Cuts at:    451    1714    2214     451 
   Size:       1263     500    2898

  Fragments arranged by size: 

                2898    1263     500
 
SspI AAT'ATT

Cuts at:    346     357     346 
   Size:         11    4650
 
StyI C'CwwG_G

Cuts at:    836    1795    2149     836 
   Size:        959     354    3348

  Fragments arranged by size: 

                3348     959     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2244    2377    2836    3045    2244 
   Size:        133     459     209    3860

  Fragments arranged by size: 

                3860     459     209     133
 
TatI w'GTAC_w

Cuts at:   1034    4176    1034 
   Size:       3142    1519
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4178    4210    4178 
   Size:         32    4629
 
Tth111I GACn'n_nGTC

Cuts at:   2458    2458 
   Size:       4661
 
XbaI T'CTAG_A

Cuts at:   1692    1692 
   Size:       4661
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1296    2156    1296 
   Size:        860    3801
 
XmaI C'CCGG_G

Cuts at:   1681    1681 
   Size:       4661
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII    BbvCI     BcgI    BfrBI    BmgBI     BmtI   Bpu10I
    BsiWI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI     SrfI     StuI     SwaI     XhoI
     XmnI     ZraI
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