Restriction map for pF1KSDA0017
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AviII       AvrII       BbeI        BbrPI       BciVI       
BcnI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsiEI       BsiWI       
BspLU11I    BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      CpoI        DraIII      
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoT22I     FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HpaI        KasI        
KspI        MluI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    StuI        SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       2075  GGTACC               G^GTACC
AccI         1388  GTCGAC               GT^[AC][GT]AC
AccIII       3632  TCCGGA               T^CCGGA
AhdI         1380  GACAGTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         710  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      2075  GGTACC               G^GTACC
AspEI        1380  GACAGTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        2558  GAGCTC               G[AT]GC[AT]^C
BcgI         1797  GCAGCGGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3272  TGATCA               T^GATCA
BglII         107  AGATCT               A^GATCT
BsaBI        3199  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        3632  TCCGGA               T^CCGGA
BseRI        1679  GAGGAG               GAGGAG(10/8)
BsiHKAI      2558  GAGCTC               G[AT]GC[AT]^C
Bsp1407I        8  TGTACA               T^GTACA
BspDI         888  ATCGAT               AT^CGAT
BspEI        3632  TCCGGA               T^CCGGA
BspHI        3275  TCATGA               T^CATGA
BsrDI        2573  GCAATG               GCAATG(2/0)
BsrGI           8  TGTACA               T^GTACA
ClaI          888  ATCGAT               AT^CGAT
DraI          648  TTTAAA               TTT^AAA
Eam1105I     1380  GACAGTGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         3272  TGATCA               T^GATCA
HgaI         1386  GCGTC                GACGC(5/10)
HincII       1388  GTCGAC               GT[CT]^[GA]AC
HindII       1388  GTCGAC               GT[CT]^[GA]AC
HindIII      2785  AAGCTT               A^AGCTT
KpnI         2075  GGTACC               GGTAC^C
MamI         3199  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1628  CAATTG               C^AATTG
MroI         3632  TCCGGA               T^CCGGA
MunI         1628  CAATTG               C^AATTG
NcoI         3551  CCATGG               C^CATGG
NdeI         3156  CATATG               CA^TATG
PaeR7I       3622  CTCGAG               C^TCGAG
PflMI        3423  CCAGCATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         247  GACTCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         3275  TCATGA               T^CATGA
SacI         2558  GAGCTC               GAGCT^C
SalI         1388  GTCGAC               G^TCGAC
SapI         2100  GCTCTTC              GCTCTTC(1/4)
ScaI          741  AGTACT               AGT^ACT
SexAI        1356  ACCTGGT              A^CC[AT]GGT
SphI         1886  GCATGC               GCATG^C
SspBI           8  TGTACA               T^GTACA
Van91I       3423  CCAGCATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          309  CCAAGAAACCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3622  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Asp700I        59  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2097  GAAGCTCTTC          
AvaI         2251  CCCGAG               C^[CT]CG[GA]G
             3622  CTCGAG              
BanI          217  GGCACC               G^G[CT][GA]CC
             2075  GGTACC              
BanII        1971  GGGCTC               G[GA]GC[CT]^C
             2558  GAGCTC              
BbvCI        3480  CCTCAGC              CCTCAGC(-5/-2)
             2022  GCTGAGG             
BfaI          637  CTAG                 C^TAG
             1447  CTAG                
BmyI         1971  GGGCTC               G[AGT]GC[ACT]^C
             2558  GAGCTC              
BsaI         3527  GAGACC               GGTCTC(1/5)
             1802  GGTCTC              
BsaWI         185  TCCGGT               [AT]^CCGG[AT]
             3632  TCCGGA              
BsmBI        3576  CGTCTC               CGTCTC(1/5)
             3295  GAGACG              
BsoBI        2251  CCCGAG               C^[CT]CG[GA]G
             3622  CTCGAG              
Bsp1286I     1971  GGGCTC               G[AGT]GC[ACT]^C
             2558  GAGCTC              
BsrBI        2151  CCGCTC               CCGCTC(-3/-3)
             2491  GAGCGG              
BstEII        784  GGTGACC              G^GT[ACGT]ACC
             1360  GGTAACC             
BstPI         784  GGTGACC              G^GT[ACGT]ACC
             1360  GGTAACC             
EarI         2101  CTCTTC               CTCTTC(1/4)
             2836  GAAGAG              
EcoNI         841  CCTGAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1277  CCTGTGGTAGG         
EcoO65I       784  GGTGACC              G^GT[ACGT]ACC
             1360  GGTAACC             
Ksp632I      2101  CTCTTC               CTCTTC(1/4)
             2836  GAAGAG              
MaeI          637  CTAG                 C^TAG
             1447  CTAG                
MaeII        3397  ACGT                 A^CGT
             3575  ACGT                
NspI         1886  GCATGC               [GA]CATG^[CT]
             3426  GCATGT              
PstI          443  CTGCAG               CTGCA^G
             2341  CTGCAG              
PvuII        1717  CAGCTG               CAG^CTG
             2641  CAGCTG              
SmlI         1324  CTTGAG               C^T[CT][GA]AG
             3622  CTCGAG              
SspI          983  AATATT               AAT^ATT
             1996  AATATT              
XmnI           59  GAAACTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2097  GAAGCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspI        AvaII       BalI        BamHI       
BbsI        BbvI        BpmI        BpuAI       BsaJI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsmI        BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DrdI        DsaI        
EaeI        EcoO109I    EcoRII      EcoRV       EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflFI       PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     XhoII       
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