Restriction map for pF1KSDA0017
 (Circular) (Six-base) MAPSORT of: pF1KSDA0017.seq  Check: 7695  from: 1  to: 6788



pF1KSDA0017  6788 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3575    3575 
   Size:       6788
 
AccI GT'mk_AC

Cuts at:   1515    3826    1515 
   Size:       2311    4477
 
Acc65I G'GTAC_C

Cuts at:   2201    3804    2201 
   Size:       1603    5185
 
AclI AA'CG_TT

Cuts at:   6628    6628 
   Size:       6788
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1820    3728    4613    5045    5792    6060    1820 
   Size:       1908     885     432     747     268    2548

  Fragments arranged by size: 

                2548    1908     885     747     432     268
 
AflIII A'CryG_T

Cuts at:   4140    5244    6221    4140 
   Size:       1104     977    4707

  Fragments arranged by size: 

                4707    1104     977
 
AgeI A'CCGG_T

Cuts at:   5176    5176 
   Size:       6788
 
AhdI GACnn_n'nnGTC

Cuts at:   1511    1511 
   Size:       6788
 
AleI CACnn'nnGTG

Cuts at:    622    1573     622 
   Size:        951    5837
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3529    3529 
   Size:       6788
 
AlwNI CAG_nnn'CTG

Cuts at:    841    4093    5660     841 
   Size:       3252    1567    1969

  Fragments arranged by size: 

                3252    1969    1567
 
ApaLI G'TGCA_C

Cuts at:   5558    5558 
   Size:       6788
 
ApoI r'AATT_y

Cuts at:   1180    2344    2485    3792    6141    6397    1180 
   Size:       1164     141    1307    2349     256    1571

  Fragments arranged by size: 

                2349    1571    1307    1164     256     141
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6788
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6788
 
AvaI C'yCGr_G

Cuts at:   2377    3748    3808    2377 
   Size:       1371      60    5357

  Fragments arranged by size: 

                5357    1371      60
 
BamHI G'GATC_C

Cuts at:    495    1830    1983    3101    3330    3813     495 
   Size:       1335     153    1118     229     483    3470

  Fragments arranged by size: 

                3470    1335    1118     483     229     153
 
BanI G'GyrC_C

Cuts at:    343    2201    3804    4218    4466    4501     343 
   Size:       1858    1603     414     248      35    2630

  Fragments arranged by size: 

                2630    1858    1603     414     248      35
 
BanII G_rGCy'C

Cuts at:   2101    2688    3802    4832    2101 
   Size:        587    1114    1030    4057

  Fragments arranged by size: 

                4057    1114    1030     587
 
BbeI G_GCGC'C

Cuts at:   4222    4470    4222 
   Size:        248    6540
 
BbsI GAAGACnn'nnnn_

Cuts at:   1741    2436    2834    3448    1741 
   Size:        695     398     614    5081

  Fragments arranged by size: 

                5081     695     614     398
 
BbvCI CC'TCA_GC

Cuts at:   2149    3607    2149 
   Size:       1458    5330
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1912    1946    1912 
   Size:         34    6754
 
BciVI GTATCCnnnnn_n'

Cuts at:   4686    5447    6788    4686 
   Size:        761    1341    4686

  Fragments arranged by size: 

                4686    1341     761
 
BclI T'GATC_A

Cuts at:   3398    4308    3398 
   Size:        910    5878
 
BglII A'GATC_T

Cuts at:    233    4303    6340     233 
   Size:       4070    2037     681

  Fragments arranged by size: 

                4070    2037     681
 
BlpI GC'TnA_GC

Cuts at:   3900    3900 
   Size:       6788
 
Bme1580I G_kGCm'C

Cuts at:   4413    4506    5562    4413 
   Size:         93    1056    5639

  Fragments arranged by size: 

                5639    1056      93
 
BmgBI CAC'GTC

Cuts at:   3524    3524 
   Size:       6788
 
BmrI ACTGGGnnnn_n'

Cuts at:   2183    4000    4081    4416    6560    2183 
   Size:       1817      81     335    2144    2411

  Fragments arranged by size: 

                2411    2144    1817     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2115    5155    2115 
   Size:       3040    3748
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    863    1937    2956    3479    3515     863 
   Size:       1074    1019     523      36    4136

  Fragments arranged by size: 

                4136    1074    1019     523      36
 
Bpu10I CC'TnA_GC

Cuts at:   2149    3607    2149 
   Size:       1458    5330
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1471    3964    4843    5335    5633    1471 
   Size:       2493     879     492     298    2626

  Fragments arranged by size: 

                2626    2493     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1934    3647    6492    1934 
   Size:       1713    2845    2230

  Fragments arranged by size: 

                2845    2230    1713
 
BsaAI yAC'GTr

Cuts at:   3780    4141    3780 
   Size:        361    6427
 
BsaBI GATnn'nnATC

Cuts at:      6    3329    4302    6339       6 
   Size:       3323     973    2037     455

  Fragments arranged by size: 

                3323    2037     973     455
 
BsaHI Gr'CG_yC

Cuts at:   4219    4467    5169    6665    4219 
   Size:        248     702    1496    4342

  Fragments arranged by size: 

                4342    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    311    3758    3977    4498    5176    5450    5597    6008     311 
   Size:       3447     219     521     678     274     147     411    1091

  Fragments arranged by size: 

                3447    1091     678     521     411     274     219     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    632     662    1580    1610    1799    1829     632 
   Size:         30     918      30     189      30    5591

  Fragments arranged by size: 

                5591     918     189      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1820    1820 
   Size:       6788
 
BseYI C'CCAG_C

Cuts at:    737    2025    2030    2085    3259    4213    4971    5548    6044 
   Size:       1288       5      55    1174     954     758     577     496

Cuts at:   6044     737 
   Size:       1481

  Fragments arranged by size: 

                1481    1288    1174     954     758     577     496      55
                   5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1696    1787    2826    1696 
   Size:         91    1039    5658

  Fragments arranged by size: 

                5658    1039      91
 
BsiEI CG_ry'CG

Cuts at:    104    4330    4376    5584     104 
   Size:       4226      46    1208    1308

  Fragments arranged by size: 

                4226    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2688    3802    4580    4770    5562    2688 
   Size:       1114     778     190     792    3914

  Fragments arranged by size: 

                3914    1114     792     778     190
 
BsmI GAATG_Cn'

Cuts at:   1569    1694    2246    2647    1569 
   Size:        125     552     401    5710

  Fragments arranged by size: 

                5710     552     401     125
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3415    3708    3415 
   Size:        293    6495
 
Bsp1286I G_dGCh'C

Cuts at:   2101    2688    3802    4413    4506    4580    4770    4832    5562 
   Size:        587    1114     611      93      74     190      62     730

Cuts at:   5562    2101 
   Size:       3327

  Fragments arranged by size: 

                3327    1114     730     611     587     190      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3758    6008    3758 
   Size:       2250    4538
 
BspHI T'CATG_A

Cuts at:   3401    5964    3401 
   Size:       2563    4225
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1668    2798    3575    3838    4052    4354    4735    1668 
   Size:       1130     777     263     214     302     381    3721

  Fragments arranged by size: 

                3721    1130     777     381     302     263     214
 
BsrBI CCG'CTC

Cuts at:     50    2279    2619    5080    5134    6618    6783      50 
   Size:       2229     340    2461      54    1484     165      55

  Fragments arranged by size: 

                2461    2229    1484     340     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2706    4700    2706 
   Size:       1994    4794
 
BsrFI r'CCGG_y

Cuts at:   4786    4967    5176    4786 
   Size:        181     209    6398

  Fragments arranged by size: 

                6398     209     181
 
BsrGI T'GTAC_A

Cuts at:    134     134 
   Size:       6788
 
BssSI C'ACGA_G

Cuts at:   5059    5417    5059 
   Size:        358    6430
 
BstAPI GCAn_nnn'nTGC

Cuts at:   4165    4165 
   Size:       6788
 
BstBI TT'CG_AA

Cuts at:    110    5149     110 
   Size:       5039    1749
 
BstEII G'GTnAC_C

Cuts at:    910    1486     910 
   Size:        576    6212
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1260    3112    3240    1260 
   Size:       1852     128    4808

  Fragments arranged by size: 

                4808    1852     128
 
BstYI r'GATC_y

Cuts at:      1     233     495    1097    1830    1983    3101    3330    3813 
   Size:        232     262     602     733     153    1118     229     483

Cuts at:   3813    4280    4303    4638    4884    5896    5982    5994    6340 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   6340       1 
   Size:        449

  Fragments arranged by size: 

                1118    1012     733     602     483     467     449     346
                 335     262     246     232     229     153      86      23
                  12
 
BtgI C'CryG_G

Cuts at:    225    3360    3677     225 
   Size:       3135     317    3336

  Fragments arranged by size: 

                3336    3135     317
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4738    4919    5082    4738 
   Size:        181     163    6444

  Fragments arranged by size: 

                6444     181     163
 
BtsI GCAGTG_nn'

Cuts at:    375    1775    2464    2713    4031     375 
   Size:       1400     689     249    1318    3132

  Fragments arranged by size: 

                3132    1400    1318     689     249
 
ClaI AT'CG_AT

Cuts at:   1015    1015 
   Size:       6788
 
DraI TTT'AAA

Cuts at:    776    3787     776 
   Size:       3011    3777
 
DrdI GACnn_nn'nnGTC

Cuts at:   1720    1963    3429    4494    5352    1720 
   Size:        243    1466    1065     858    3156

  Fragments arranged by size: 

                3156    1466    1065     858     243
 
EaeI y'GGCC_r

Cuts at:    477    1370    1901    2264    2390    2627    2852    4373    4547 
   Size:        893     531     363     126     237     225    1521     174

Cuts at:   4547    4938    4965    6310     477 
   Size:        391      27    1345     955

  Fragments arranged by size: 

                1521    1345     955     893     531     391     363     237
                 225     174     126      27
 
EagI C'GGCC_G

Cuts at:   4373    4373 
   Size:       6788
 
EarI CTCTTCn'nnn_

Cuts at:   2233    2957    4811    5021    2233 
   Size:        724    1854     210    4000

  Fragments arranged by size: 

                4000    1854     724     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    963    4996    5306    5452    6598     963 
   Size:       4033     310     146    1146    1153

  Fragments arranged by size: 

                4033    1153    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2686    3800    2686 
   Size:       1114    5674
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    863    1820    1937    2956    3479    3515    3728    4613    5045 
   Size:        957     117    1019     523      36     213     885     432

Cuts at:   5045    5792    6060     863 
   Size:        747     268    1591

  Fragments arranged by size: 

                1591    1019     957     885     747     523     432     268
                 213     117      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:    971    1407     971 
   Size:        436    6352
 
EcoO109I rG'GnC_Cy

Cuts at:   1414    2210    2859    2967    3377    3927    1414 
   Size:        796     649     108     410     550    4275

  Fragments arranged by size: 

                4275     796     649     550     410     108
 
EcoRI G'AATT_C

Cuts at:   3792    3792 
   Size:       6788
 
EcoRV GAT'ATC

Cuts at:    924    1053    3756     924 
   Size:        129    2703    3956

  Fragments arranged by size: 

                3956    2703     129
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4807    4807 
   Size:       6788
 
FspI TGC'GCA

Cuts at:   4569    4569 
   Size:       6788
 
HaeII r_GCGC'y

Cuts at:   4222    4470    5492    6471    4222 
   Size:        248    1022     979    4539

  Fragments arranged by size: 

                4539    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    632     664     857     889    1231    1263    1321    1350    1353 
   Size:         32     193      32     342      32      58      29       3

Cuts at:   1353    1382    1497    1529    1799    1831    1912    1944    4630 
   Size:         29     115      32     270      32      81      32    2686

Cuts at:   4630    4660    4662    4692     632 
   Size:         30       2      30    2728

  Fragments arranged by size: 

                2728    2686     342     270     193     115      81      58
                  32      32      32      32      32      32      30      30
                  29      29       3       2
 
HincII GTy'rAC

Cuts at:   1516    3827    1516 
   Size:       2311    4477
 
HindIII A'AGCT_T

Cuts at:   2911    2911 
   Size:       6788
 
KasI G'GCGC_C

Cuts at:   4218    4466    4218 
   Size:        248    6540
 
KpnI G_GTAC'C

Cuts at:   2205    3808    2205 
   Size:       1603    5185
 
MfeI C'AATT_G

Cuts at:   1754    1754 
   Size:       6788
 
MluI A'CGCG_T

Cuts at:   6221    6221 
   Size:       6788
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    618     630     736    1307    2840    5459    5643     618 
   Size:         12     106     571    1533    2619     184    1763

  Fragments arranged by size: 

                2619    1763    1533     571     184     106      12
 
MscI TGG'CCA

Cuts at:    479    1372    1903    2266    2392    2854    4549     479 
   Size:        893     531     363     126     462    1695    2718

  Fragments arranged by size: 

                2718    1695     893     531     462     363     126
 
MslI CAynn'nnrTG

Cuts at:    622    1144    1549    1573    3286    3511    4777    4904     622 
   Size:        522     405      24    1713     225    1266     127    2506

  Fragments arranged by size: 

                2506    1713    1266     522     405     225     127      24
            
 
MspA1I CmG'CkG

Cuts at:    929    1845    1926    2769    3899    4213    4573    5586    5831 
   Size:        916      81     843    1130     314     360    1013     245

Cuts at:   5831     929 
   Size:       1886

  Fragments arranged by size: 

                1886    1130    1013     916     843     360     314     245
                  81
 
NaeI GCC'GGC

Cuts at:   4969    4969 
   Size:       6788
 
NarI GG'CG_CC

Cuts at:   4219    4467    4219 
   Size:        248    6540
 
NcoI C'CATG_G

Cuts at:   3677    3677 
   Size:       6788
 
NdeI CA'TA_TG

Cuts at:   3283    3283 
   Size:       6788
 
NgoMIV G'CCGG_C

Cuts at:   4967    4967 
   Size:       6788
 
NspI r_CATG'y

Cuts at:   2016    3556    3841    4341    5248    2016 
   Size:       1540     285     500     907    3556

  Fragments arranged by size: 

                3556    1540     907     500     285
 
PciI A'CATG_T

Cuts at:   5244    5244 
   Size:       6788
 
PflMI CCAn_nnn'nTGG

Cuts at:   3555    3555 
   Size:       6788
 
PfoI T'CCnGG_A

Cuts at:    840    3239     840 
   Size:       2399    4389
 
PmeI GTTT'AAAC

Cuts at:   3787    3787 
   Size:       6788
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5927    5959    5927 
   Size:         32    6756
 
PpuMI rG'GwC_Cy

Cuts at:   1414    2210    2859    2967    1414 
   Size:        796     649     108    5235

  Fragments arranged by size: 

                5235     796     649     108
 
PshAI GACnn'nnGTC

Cuts at:    377     377 
   Size:       6788
 
PspXI vC'TCGA_Gb

Cuts at:   3748    3748 
   Size:       6788
 
PstI C_TGCA'G

Cuts at:    573    2471    3835    4520     573 
   Size:       1898    1364     685    2841

  Fragments arranged by size: 

                2841    1898    1364     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6788
 
PvuII CAG'CTG

Cuts at:   1845    2769    4213    4573    1845 
   Size:        924    1444     360    4060

  Fragments arranged by size: 

                4060    1444     924     360
 
RsrII CG'GwC_CG

Cuts at:   4983    4983 
   Size:       6788
 
SacI G_AGCT'C

Cuts at:   2688    3802    2688 
   Size:       1114    5674
 
SalI G'TCGA_C

Cuts at:   1514    3825    1514 
   Size:       2311    4477
 
SanDI GG'GwC_CC

Cuts at:   1414    2210    1414 
   Size:        796    5992
 
SapI GCTCTTCn'nnn_

Cuts at:   2233    4811    5021    2233 
   Size:       2578     210    4000

  Fragments arranged by size: 

                4000    2578     210
 
SbfI CC_TGCA'GG

Cuts at:   3835    3835 
   Size:       6788
 
ScaI AGT'ACT

Cuts at:    869    6305     869 
   Size:       5436    1352
 
SexAI A'CCwGG_T

Cuts at:   1482    1482 
   Size:       6788
 
SfcI C'TryA_G

Cuts at:     33     569    1595    2131    2467    3227    3335    3831    4516 
   Size:        536    1026     536     336     760     108     496     685

Cuts at:   4516    5509    5700      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121    1026     993     760     685     536     536     496
                 336     191     108
 
SfoI GGC'GCC

Cuts at:   4220    4468    4220 
   Size:        248    6540
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6788
 
SmaI CCC'GGG

Cuts at:   3810    3810 
   Size:       6788
 
SmlI C'TyrA_G

Cuts at:   1450    3748    3943    4858    5350    5612    1450 
   Size:       2298     195     915     492     262    2626

  Fragments arranged by size: 

                2626    2298     915     492     262     195
 
SnaBI TAC'GTA

Cuts at:   3780    3780 
   Size:       6788
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6788
 
SphI G_CATG'C

Cuts at:   2016    3841    4341    2016 
   Size:       1825     500    4463

  Fragments arranged by size: 

                4463    1825     500
 
SspI AAT'ATT

Cuts at:   1111    2124    1111 
   Size:       1013    5775
 
StyI C'CwwG_G

Cuts at:    768    1670    2690    2855    3677    3922    4276     768 
   Size:        902    1020     165     822     245     354    3280

  Fragments arranged by size: 

                3280    1020     902     822     354     245     165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2050    2077    2493    2836    3734    4371    4504    4963    5172 
   Size:         27     416     343     898     637     133     459     209

Cuts at:   5172    2050 
   Size:       3666

  Fragments arranged by size: 

                3666     898     637     459     416     343     209     133
                  27
 
TatI w'GTAC_w

Cuts at:    134     273     867    1853    2471    6303     134 
   Size:        139     594     986     618    3832     619

  Fragments arranged by size: 

                3832     986     619     618     594     139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1439    1471    6305    6337    1439 
   Size:         32    4834      32    1890

  Fragments arranged by size: 

                4834    1890      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1641    2167    2761    4585    1641 
   Size:        526     594    1824    3844

  Fragments arranged by size: 

                3844    1824     594     526
 
XbaI T'CTAG_A

Cuts at:   3819    3819 
   Size:       6788
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    442    4283     442 
   Size:       3841    2947
 
XhoI C'TCGA_G

Cuts at:   3748    3748 
   Size:       6788
 
XmaI C'CCGG_G

Cuts at:   3808    3808 
   Size:       6788
 
XmnI GAAnn'nnTTC

Cuts at:    189    2227     189 
   Size:       2038    4750
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AloI     ApaI     AscI    AvrII     BaeI
    BfrBI     BglI     BmtI    BsiWI   BssHII  BstZ17I   Bsu36I    CspCI
   DraIII     FseI    FspAI     HpaI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmlI     PsiI   PspOMI     PsrI    SacII     SfiI
    SgrAI     SrfI     StuI     SwaI     ZraI
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