Restriction map for pF1KSDA0017
 (Linear) (Six-base) MAPSORT of: KIAA0017.seq  Check: 7352  from: 1  to: 3651



KIAA0017.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3449    3651 
   Size:       3449     202
 
AccI GT'mk_AC

Cuts at:      0    1389    3651 
   Size:       1389    2262
 
Acc65I G'GTAC_C

Cuts at:      0    2075    3651 
   Size:       2075    1576
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1694    3602    3651 
   Size:       1694    1908      49

  Fragments arranged by size: 

                1908    1694      49
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1385    3651 
   Size:       1385    2266
 
AleI CACnn'nnGTG

Cuts at:      0     496    1447    3651 
   Size:        496     951    2204

  Fragments arranged by size: 

                2204     951     496
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3403    3651 
   Size:       3403     248
 
AlwNI CAG_nnn'CTG

Cuts at:      0     715    3651 
   Size:        715    2936
 
ApoI r'AATT_y

Cuts at:      0    1054    2218    2359    3651 
   Size:       1054    1164     141    1292

  Fragments arranged by size: 

                1292    1164    1054     141
 
AvaI C'yCGr_G

Cuts at:      0    2251    3622    3651 
   Size:       2251    1371      29

  Fragments arranged by size: 

                2251    1371      29
 
BamHI G'GATC_C

Cuts at:      0     369    1704    1857    2975    3204    3651 
   Size:        369    1335     153    1118     229     447

  Fragments arranged by size: 

                1335    1118     447     369     229     153
 
BanI G'GyrC_C

Cuts at:      0     217    2075    3651 
   Size:        217    1858    1576

  Fragments arranged by size: 

                1858    1576     217
 
BanII G_rGCy'C

Cuts at:      0    1975    2562    3651 
   Size:       1975     587    1089

  Fragments arranged by size: 

                1975    1089     587
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1615    2310    2708    3322    3651 
   Size:       1615     695     398     614     329

  Fragments arranged by size: 

                1615     695     614     398     329
 
BbvCI CC'TCA_GC

Cuts at:      0    2023    3481    3651 
   Size:       2023    1458     170

  Fragments arranged by size: 

                2023    1458     170
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1786    1820    3651 
   Size:       1786      34    1831

  Fragments arranged by size: 

                1831    1786      34
 
BclI T'GATC_A

Cuts at:      0    3272    3651 
   Size:       3272     379
 
BglII A'GATC_T

Cuts at:      0     107    3651 
   Size:        107    3544
 
BmgBI CAC'GTC

Cuts at:      0    3398    3651 
   Size:       3398     253
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2057    3651 
   Size:       2057    1594
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1989    3651 
   Size:       1989    1662
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    1811    2830    3353    3389    3651 
   Size:        737    1074    1019     523      36     262

  Fragments arranged by size: 

                1074    1019     737     523     262      36
 
Bpu10I CC'TnA_GC

Cuts at:      0    2023    3481    3651 
   Size:       2023    1458     170

  Fragments arranged by size: 

                2023    1458     170
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1345    3651 
   Size:       1345    2306
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1808    3521    3651 
   Size:       1808    1713     130

  Fragments arranged by size: 

                1808    1713     130
 
BsaBI GATnn'nnATC

Cuts at:      0    3203    3651 
   Size:       3203     448
 
BsaWI w'CCGG_w

Cuts at:      0     185    3632    3651 
   Size:        185    3447      19

  Fragments arranged by size: 

                3447     185      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     506     536    1454    1484    1673    1703    3651 
   Size:        506      30     918      30     189      30    1948

  Fragments arranged by size: 

                1948     918     506     189      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1694    3651 
   Size:       1694    1957
 
BseYI C'CCAG_C

Cuts at:      0     611    1899    1904    1959    3133    3651 
   Size:        611    1288       5      55    1174     518

  Fragments arranged by size: 

                1288    1174     611     518      55       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1570    1661    2700    3651 
   Size:       1570      91    1039     951

  Fragments arranged by size: 

                1570    1039     951      91
 
BsiHKAI G_wGCw'C

Cuts at:      0    2562    3651 
   Size:       2562    1089
 
BsmI GAATG_Cn'

Cuts at:      0    1443    1568    2120    2521    3651 
   Size:       1443     125     552     401    1130

  Fragments arranged by size: 

                1443    1130     552     401     125
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3289    3582    3651 
   Size:       3289     293      69

  Fragments arranged by size: 

                3289     293      69
 
Bsp1286I G_dGCh'C

Cuts at:      0    1975    2562    3651 
   Size:       1975     587    1089

  Fragments arranged by size: 

                1975    1089     587
 
BspEI T'CCGG_A

Cuts at:      0    3632    3651 
   Size:       3632      19
 
BspHI T'CATG_A

Cuts at:      0    3275    3651 
   Size:       3275     376
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1542    2672    3449    3651 
   Size:       1542    1130     777     202

  Fragments arranged by size: 

                1542    1130     777     202
 
BsrBI CCG'CTC

Cuts at:      0    2153    2493    3651 
   Size:       2153     340    1158

  Fragments arranged by size: 

                2153    1158     340
 
BsrDI GCAATG_nn'

Cuts at:      0    2580    3651 
   Size:       2580    1071
 
BsrGI T'GTAC_A

Cuts at:      0       8    3651 
   Size:          8    3643
 
BstEII G'GTnAC_C

Cuts at:      0     784    1360    3651 
   Size:        784     576    2291

  Fragments arranged by size: 

                2291     784     576
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1134    2986    3114    3651 
   Size:       1134    1852     128     537

  Fragments arranged by size: 

                1852    1134     537     128
 
BstYI r'GATC_y

Cuts at:      0     107     369     971    1704    1857    2975    3204    3651 
   Size:        107     262     602     733     153    1118     229     447

  Fragments arranged by size: 

                1118     733     602     447     262     229     153     107
            
 
BtgI C'CryG_G

Cuts at:      0      99    3234    3551    3651 
   Size:         99    3135     317     100

  Fragments arranged by size: 

                3135     317     100      99
 
BtsI GCAGTG_nn'

Cuts at:      0     249    1649    2338    2587    3651 
   Size:        249    1400     689     249    1064

  Fragments arranged by size: 

                1400    1064     689     249     249
 
ClaI AT'CG_AT

Cuts at:      0     889    3651 
   Size:        889    2762
 
DraI TTT'AAA

Cuts at:      0     650    3651 
   Size:        650    3001
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1594    1837    3303    3651 
   Size:       1594     243    1466     348

  Fragments arranged by size: 

                1594    1466     348     243
 
EaeI y'GGCC_r

Cuts at:      0     351    1244    1775    2138    2264    2501    2726    3651 
   Size:        351     893     531     363     126     237     225     925

  Fragments arranged by size: 

                 925     893     531     363     351     237     225     126
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    2107    2831    3651 
   Size:       2107     724     820

  Fragments arranged by size: 

                2107     820     724
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     837    3651 
   Size:        837    2814
 
EcoICRI GAG'CTC

Cuts at:      0    2560    3651 
   Size:       2560    1091
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     737    1694    1811    2830    3353    3389    3602    3651 
   Size:        737     957     117    1019     523      36     213      49

  Fragments arranged by size: 

                1019     957     737     523     213     117      49      36
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     845    1281    3651 
   Size:        845     436    2370

  Fragments arranged by size: 

                2370     845     436
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1288    2084    2733    2841    3251    3651 
   Size:       1288     796     649     108     410     400

  Fragments arranged by size: 

                1288     796     649     410     400     108
 
EcoRV GAT'ATC

Cuts at:      0     798     927    3630    3651 
   Size:        798     129    2703      21

  Fragments arranged by size: 

                2703     798     129      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     506     538     731     763    1105    1137    1195    1224 
   Size:        506      32     193      32     342      32      58      29

Cuts at:   1224    1227    1256    1371    1403    1673    1705    1786    1818 
   Size:          3      29     115      32     270      32      81      32

Cuts at:   1818    3651 
   Size:       1833

  Fragments arranged by size: 

                1833     506     342     270     193     115      81      58
                  32      32      32      32      32      32      29      29
                   3
 
HincII GTy'rAC

Cuts at:      0    1390    3651 
   Size:       1390    2261
 
HindIII A'AGCT_T

Cuts at:      0    2785    3651 
   Size:       2785     866
 
KpnI G_GTAC'C

Cuts at:      0    2079    3651 
   Size:       2079    1572
 
MfeI C'AATT_G

Cuts at:      0    1628    3651 
   Size:       1628    2023
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     492     504     610    1181    2714    3651 
   Size:        492      12     106     571    1533     937

  Fragments arranged by size: 

                1533     937     571     492     106      12
 
MscI TGG'CCA

Cuts at:      0     353    1246    1777    2140    2266    2728    3651 
   Size:        353     893     531     363     126     462     923

  Fragments arranged by size: 

                 923     893     531     462     363     353     126
 
MslI CAynn'nnrTG

Cuts at:      0     496    1018    1423    1447    3160    3385    3651 
   Size:        496     522     405      24    1713     225     266

  Fragments arranged by size: 

                1713     522     496     405     266     225      24
 
MspA1I CmG'CkG

Cuts at:      0     803    1719    1800    2643    3651 
   Size:        803     916      81     843    1008

  Fragments arranged by size: 

                1008     916     843     803      81
 
NcoI C'CATG_G

Cuts at:      0    3551    3651 
   Size:       3551     100
 
NdeI CA'TA_TG

Cuts at:      0    3157    3651 
   Size:       3157     494
 
NspI r_CATG'y

Cuts at:      0    1890    3430    3651 
   Size:       1890    1540     221

  Fragments arranged by size: 

                1890    1540     221
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3429    3651 
   Size:       3429     222
 
PfoI T'CCnGG_A

Cuts at:      0     714    3113    3651 
   Size:        714    2399     538

  Fragments arranged by size: 

                2399     714     538
 
PpuMI rG'GwC_Cy

Cuts at:      0    1288    2084    2733    2841    3651 
   Size:       1288     796     649     108     810

  Fragments arranged by size: 

                1288     810     796     649     108
 
PshAI GACnn'nnGTC

Cuts at:      0     251    3651 
   Size:        251    3400
 
PspXI vC'TCGA_Gb

Cuts at:      0    3622    3651 
   Size:       3622      29
 
PstI C_TGCA'G

Cuts at:      0     447    2345    3651 
   Size:        447    1898    1306

  Fragments arranged by size: 

                1898    1306     447
 
PvuII CAG'CTG

Cuts at:      0    1719    2643    3651 
   Size:       1719     924    1008

  Fragments arranged by size: 

                1719    1008     924
 
SacI G_AGCT'C

Cuts at:      0    2562    3651 
   Size:       2562    1089
 
SalI G'TCGA_C

Cuts at:      0    1388    3651 
   Size:       1388    2263
 
SanDI GG'GwC_CC

Cuts at:      0    1288    2084    3651 
   Size:       1288     796    1567

  Fragments arranged by size: 

                1567    1288     796
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2107    3651 
   Size:       2107    1544
 
ScaI AGT'ACT

Cuts at:      0     743    3651 
   Size:        743    2908
 
SexAI A'CCwGG_T

Cuts at:      0    1356    3651 
   Size:       1356    2295
 
SfcI C'TryA_G

Cuts at:      0     443    1469    2005    2341    3101    3209    3651 
   Size:        443    1026     536     336     760     108     442

  Fragments arranged by size: 

                1026     760     536     443     442     336     108
 
SmlI C'TyrA_G

Cuts at:      0    1324    3622    3651 
   Size:       1324    2298      29

  Fragments arranged by size: 

                2298    1324      29
 
SphI G_CATG'C

Cuts at:      0    1890    3651 
   Size:       1890    1761
 
SspI AAT'ATT

Cuts at:      0     985    1998    3651 
   Size:        985    1013    1653

  Fragments arranged by size: 

                1653    1013     985
 
StyI C'CwwG_G

Cuts at:      0     642    1544    2564    2729    3551    3651 
   Size:        642     902    1020     165     822     100

  Fragments arranged by size: 

                1020     902     822     642     165     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1924    1951    2367    2710    3608    3651 
   Size:       1924      27     416     343     898      43

  Fragments arranged by size: 

                1924     898     416     343      43      27
 
TatI w'GTAC_w

Cuts at:      0       8     147     741    1727    2345    3651 
   Size:          8     139     594     986     618    1306

  Fragments arranged by size: 

                1306     986     618     594     139       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1313    1345    3651 
   Size:       1313      32    2306

  Fragments arranged by size: 

                2306    1313      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1515    2041    2635    3651 
   Size:       1515     526     594    1016

  Fragments arranged by size: 

                1515    1016     594     526
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     316    3651 
   Size:        316    3335
 
XhoI C'TCGA_G

Cuts at:      0    3622    3651 
   Size:       3622      29
 
XmnI GAAnn'nnTTC

Cuts at:      0      63    2101    3651 
   Size:         63    2038    1550

  Fragments arranged by size: 

                2038    1550      63
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI     AlfI    AlwNI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI
     BclI    BglII    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI     DraI
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NspI    PflMI     PfoI    PpuMI
    PshAI    PspXI     PstI    PvuII     SacI     SalI    SanDI     SapI
     ScaI    SexAI     SfcI     SmlI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI
    BfrBI     BglI     BlpI Bme1580I     BmtI    BsaAI    BsaHI    BsiEI
    BsiWI    BsrFI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI    CspCI   DraIII     EagI    EcoRI     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     SwaI     XbaI     XmaI     ZraI
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