Restriction map for pF1KSDA0016
 (Circular) (Six-base) MAPSORT of: pF1KSDA0016.seq  Check: 6681  from: 1  to: 3572



pF1KSDA0016  3572 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:32  ..

AccI GT'mk_AC

Cuts at:    395     610     395 
   Size:        215    3357
 
Acc65I G'GTAC_C

Cuts at:    588     588 
   Size:       3572
 
AclI AA'CG_TT

Cuts at:   3412    3412 
   Size:       3572
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     566    1397    1829    2576    2844     335 
   Size:        231     831     432     747     268    1063

  Fragments arranged by size: 

                1063     831     747     432     268     231
 
AflIII A'CryG_T

Cuts at:    924    2028    3005     924 
   Size:       1104     977    1491

  Fragments arranged by size: 

                1491    1104     977
 
AgeI A'CCGG_T

Cuts at:   1960    1960 
   Size:       3572
 
AlwNI CAG_nnn'CTG

Cuts at:    444     877    2444     444 
   Size:        433    1567    1572

  Fragments arranged by size: 

                1572    1567     433
 
ApaI G_GGCC'C

Cuts at:    171     171 
   Size:       3572
 
ApaLI G'TGCA_C

Cuts at:   2342    2342 
   Size:       3572
 
ApoI r'AATT_y

Cuts at:    576    2925    3181     576 
   Size:       2349     256     967

  Fragments arranged by size: 

                2349     967     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3572
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3572
 
AvaI C'yCGr_G

Cuts at:    592     592 
   Size:       3572
 
BamHI G'GATC_C

Cuts at:    597     597 
   Size:       3572
 
BanI G'GyrC_C

Cuts at:    588    1002    1250    1285     588 
   Size:        414     248      35    2875

  Fragments arranged by size: 

                2875     414     248      35
 
BanII G_rGCy'C

Cuts at:    171     586    1616     171 
   Size:        415    1030    2127

  Fragments arranged by size: 

                2127    1030     415
 
BbeI G_GCGC'C

Cuts at:   1006    1254    1006 
   Size:        248    3324
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    177     211     177 
   Size:         34    3538
 
BciVI GTATCCnnnnn_n'

Cuts at:   1470    2231    3572    1470 
   Size:        761    1341    1470

  Fragments arranged by size: 

                1470    1341     761
 
BclI T'GATC_A

Cuts at:   1092    1092 
   Size:       3572
 
BglII A'GATC_T

Cuts at:   1087    3124    1087 
   Size:       2037    1535
 
BlpI GC'TnA_GC

Cuts at:    684     684 
   Size:       3572
 
Bme1580I G_kGCm'C

Cuts at:    171    1197    1290    2346     171 
   Size:       1026      93    1056    1397

  Fragments arranged by size: 

                1397    1056    1026      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    784     865    1200    3344     784 
   Size:         81     335    2144    1012

  Fragments arranged by size: 

                2144    1012     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1939    1939 
   Size:       3572
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    356     748    1627    2119    2417     356 
   Size:        392     879     492     298    1511

  Fragments arranged by size: 

                1511     879     492     392     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3276    3276 
   Size:       3572
 
BsaAI yAC'GTr

Cuts at:    564     925     564 
   Size:        361    3211
 
BsaBI GATnn'nnATC

Cuts at:      6    1086    3123       6 
   Size:       1080    2037     455

  Fragments arranged by size: 

                2037    1080     455
 
BsaHI Gr'CG_yC

Cuts at:   1003    1251    1953    3449    1003 
   Size:        248     702    1496    1126

  Fragments arranged by size: 

                1496    1126     702     248
 
BsaWI w'CCGG_w

Cuts at:    761    1282    1960    2234    2381    2792     761 
   Size:        521     678     274     147     411    1541

  Fragments arranged by size: 

                1541     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    280     997    1755    2332    2828     280 
   Size:        717     758     577     496    1024

  Fragments arranged by size: 

                1024     758     717     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     201    1114    1160    2368     104 
   Size:         97     913      46    1208    1308

  Fragments arranged by size: 

                1308    1208     913      97      46
 
BsiHKAI G_wGCw'C

Cuts at:    534     586    1364    1554    2346     534 
   Size:         52     778     190     792    1760

  Fragments arranged by size: 

                1760     792     778     190      52
 
Bsp1286I G_dGCh'C

Cuts at:    171     534     586    1197    1290    1364    1554    1616    2346 
   Size:        363      52     611      93      74     190      62     730

Cuts at:   2346     171 
   Size:       1397

  Fragments arranged by size: 

                1397     730     611     363     190      93      74      62
                  52
 
BspEI T'CCGG_A

Cuts at:   2792    2792 
   Size:       3572
 
BspHI T'CATG_A

Cuts at:   2748    2748 
   Size:       3572
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    438     622     836    1138    1519     438 
   Size:        184     214     302     381    2491

  Fragments arranged by size: 

                2491     381     302     214     184
 
BsrBI CCG'CTC

Cuts at:     50    1864    1918    3402    3567      50 
   Size:       1814      54    1484     165      55

  Fragments arranged by size: 

                1814    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1484    1484 
   Size:       3572
 
BsrFI r'CCGG_y

Cuts at:    154    1570    1751    1960     154 
   Size:       1416     181     209    1766

  Fragments arranged by size: 

                1766    1416     209     181
 
BssSI C'ACGA_G

Cuts at:   1843    2201    1843 
   Size:        358    3214
 
BstAPI GCAn_nnn'nTGC

Cuts at:    444     949     444 
   Size:        505    3067
 
BstBI TT'CG_AA

Cuts at:    110    1933     110 
   Size:       1823    1749
 
BstYI r'GATC_y

Cuts at:      1     597    1064    1087    1422    1668    2680    2766    2778 
   Size:        596     467      23     335     246    1012      86      12

Cuts at:   2778    3124       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     596     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       3572
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    132    1522    1703    1866     132 
   Size:       1390     181     163    1838

  Fragments arranged by size: 

                1838    1390     181     163
 
BtsI GCAGTG_nn'

Cuts at:    815     815 
   Size:       3572
 
DraI TTT'AAA

Cuts at:    571     571 
   Size:       3572
 
DrdI GACnn_nn'nnGTC

Cuts at:   1278    2136    1278 
   Size:        858    2714
 
EaeI y'GGCC_r

Cuts at:   1157    1331    1722    1749    3094    1157 
   Size:        174     391      27    1345    1635

  Fragments arranged by size: 

                1635    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1157    1157 
   Size:       3572
 
EarI CTCTTCn'nnn_

Cuts at:    353     470    1595    1805     353 
   Size:        117    1125     210    2120

  Fragments arranged by size: 

                2120    1125     210     117
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1780    2090    2236    3382    1780 
   Size:        310     146    1146    1970

  Fragments arranged by size: 

                1970    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    584     584 
   Size:       3572
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     566    1397    1829    2576    2844     335 
   Size:        231     831     432     747     268    1063

  Fragments arranged by size: 

                1063     831     747     432     268     231
 
EcoO109I rG'GnC_Cy

Cuts at:    167     168     711     167 
   Size:          1     543    3028

  Fragments arranged by size: 

                3028     543       1
 
EcoRI G'AATT_C

Cuts at:    576     576 
   Size:       3572
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1591    1591 
   Size:       3572
 
FspI TGC'GCA

Cuts at:   1353    1353 
   Size:       3572
 
HaeII r_GCGC'y

Cuts at:    146    1006    1254    2276    3255     146 
   Size:        860     248    1022     979     463

  Fragments arranged by size: 

                1022     979     860     463     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1414    1444    1446    1476    1414 
   Size:         30       2      30    3510

  Fragments arranged by size: 

                3510      30      30       2
 
HincII GTy'rAC

Cuts at:    611     611 
   Size:       3572
 
HindIII A'AGCT_T

Cuts at:    262     262 
   Size:       3572
 
KasI G'GCGC_C

Cuts at:   1002    1250    1002 
   Size:        248    3324
 
KpnI G_GTAC'C

Cuts at:    592     592 
   Size:       3572
 
MfeI C'AATT_G

Cuts at:    413     413 
   Size:       3572
 
MluI A'CGCG_T

Cuts at:   3005    3005 
   Size:       3572
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    115    2243    2427     115 
   Size:       2128     184    1260

  Fragments arranged by size: 

                2128    1260     184
 
MscI TGG'CCA

Cuts at:   1333    1333 
   Size:       3572
 
MslI CAynn'nnrTG

Cuts at:   1561    1688    1561 
   Size:        127    3445
 
MspA1I CmG'CkG

Cuts at:    683     997    1357    2370    2615     683 
   Size:        314     360    1013     245    1640

  Fragments arranged by size: 

                1640    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1753    1753 
   Size:       3572
 
NarI GG'CG_CC

Cuts at:   1003    1251    1003 
   Size:        248    3324
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       3572
 
NgoMIV G'CCGG_C

Cuts at:   1751    1751 
   Size:       3572
 
NspI r_CATG'y

Cuts at:    625    1125    2032     625 
   Size:        500     907    2165

  Fragments arranged by size: 

                2165     907     500
 
PciI A'CATG_T

Cuts at:   2028    2028 
   Size:       3572
 
PmeI GTTT'AAAC

Cuts at:    571     571 
   Size:       3572
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2711    2743    2711 
   Size:         32    3540
 
PspOMI G'GGCC_C

Cuts at:    167     167 
   Size:       3572
 
PstI C_TGCA'G

Cuts at:    449     619    1304     449 
   Size:        170     685    2717

  Fragments arranged by size: 

                2717     685     170
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3572
 
PvuII CAG'CTG

Cuts at:    997    1357     997 
   Size:        360    3212
 
RsrII CG'GwC_CG

Cuts at:   1767    1767 
   Size:       3572
 
SacI G_AGCT'C

Cuts at:    586     586 
   Size:       3572
 
SalI G'TCGA_C

Cuts at:    609     609 
   Size:       3572
 
SapI GCTCTTCn'nnn_

Cuts at:   1595    1805    1595 
   Size:        210    3362
 
SbfI CC_TGCA'GG

Cuts at:    619     619 
   Size:       3572
 
ScaI AGT'ACT

Cuts at:   3089    3089 
   Size:       3572
 
SfcI C'TryA_G

Cuts at:     33     445     615    1300    2293    2484      33 
   Size:        412     170     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     412     191     170
 
SfoI GGC'GCC

Cuts at:   1004    1252    1004 
   Size:        248    3324
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3572
 
SgrAI Cr'CCGG_yG

Cuts at:    154     154 
   Size:       3572
 
SmaI CCC'GGG

Cuts at:    594     594 
   Size:       3572
 
SmlI C'TyrA_G

Cuts at:    371     727    1642    2134    2396     371 
   Size:        356     915     492     262    1547

  Fragments arranged by size: 

                1547     915     492     356     262
 
SnaBI TAC'GTA

Cuts at:    564     564 
   Size:       3572
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3572
 
SphI G_CATG'C

Cuts at:    625    1125     625 
   Size:        500    3072
 
StyI C'CwwG_G

Cuts at:    125     269     706    1060     125 
   Size:        144     437     354    2637

  Fragments arranged by size: 

                2637     437     354     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1155    1288    1747    1956    1155 
   Size:        133     459     209    2771

  Fragments arranged by size: 

                2771     459     209     133
 
TatI w'GTAC_w

Cuts at:   3087    3087 
   Size:       3572
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    464     496    3089    3121     464 
   Size:         32    2593      32     915

  Fragments arranged by size: 

                2593     915      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1369    1369 
   Size:       3572
 
XbaI T'CTAG_A

Cuts at:    603     603 
   Size:       3572
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1067    1067 
   Size:       3572
 
XmaI C'CCGG_G

Cuts at:    592     592 
   Size:       3572
 
XmnI GAAnn'nnTTC

Cuts at:    320     331     320 
   Size:         11    3561
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI     HpaI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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