Restriction map for pF1KSDA0016
 (Linear) (Six-base) MAPSORT of: KIAA0016.seq  Check: 5363  from: 1  to: 435



KIAA0016.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0     269     435 
   Size:        269     166
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     435 
   Size:        209     226
 
AlwNI CAG_nnn'CTG

Cuts at:      0     318     435 
   Size:        318     117
 
ApaI G_GGCC'C

Cuts at:      0      45     435 
   Size:         45     390
 
BanII G_rGCy'C

Cuts at:      0      45     435 
   Size:         45     390
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      51      85     435 
   Size:         51      34     350

  Fragments arranged by size: 

                 350      51      34
 
Bme1580I G_kGCm'C

Cuts at:      0      45     435 
   Size:         45     390
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     435 
   Size:        230     205
 
BseYI C'CCAG_C

Cuts at:      0     154     435 
   Size:        154     281
 
BsiEI CG_ry'CG

Cuts at:      0      75     435 
   Size:         75     360
 
BsiHKAI G_wGCw'C

Cuts at:      0     408     435 
   Size:        408      27
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     408     435 
   Size:         45     363      27

  Fragments arranged by size: 

                 363      45      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     312     435 
   Size:        312     123
 
BsrFI r'CCGG_y

Cuts at:      0      28     435 
   Size:         28     407
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     318     435 
   Size:        318     117
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       6     435 
   Size:          6     429
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     344     435 
   Size:        227     117      91

  Fragments arranged by size: 

                 227     117      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     435 
   Size:        209     226
 
EcoO109I rG'GnC_Cy

Cuts at:      0      41      42     435 
   Size:         41       1     393

  Fragments arranged by size: 

                 393      41       1
 
HaeII r_GCGC'y

Cuts at:      0      20     435 
   Size:         20     415
 
HindIII A'AGCT_T

Cuts at:      0     136     435 
   Size:        136     299
 
MfeI C'AATT_G

Cuts at:      0     287     435 
   Size:        287     148
 
PspOMI G'GGCC_C

Cuts at:      0      41     435 
   Size:         41     394
 
PstI C_TGCA'G

Cuts at:      0     323     435 
   Size:        323     112
 
SfcI C'TryA_G

Cuts at:      0     319     435 
   Size:        319     116
 
SgrAI Cr'CCGG_yG

Cuts at:      0      28     435 
   Size:         28     407
 
SmlI C'TyrA_G

Cuts at:      0     245     435 
   Size:        245     190
 
StyI C'CwwG_G

Cuts at:      0     143     435 
   Size:        143     292
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     338     370     435 
   Size:        338      32      65

  Fragments arranged by size: 

                 338      65      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     194     205     435 
   Size:        194      11     230

  Fragments arranged by size: 

                 230     194      11
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI    BanII     BcgI Bme1580I    BpuEI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI    BtgZI
     EarI  Eco57MI EcoO109I    HaeII  HindIII     MfeI   PspOMI     PstI
     SfcI    SgrAI     SmlI     StyI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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