Restriction map for pF1KSDA0007
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
Alw44I      AocI        Aor51HI     ApaLI       AscI        
Asp700I     AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraIII      DsaI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       FbaI        
FseI        FspI        HgaI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          598  GTCTAC               GT^[AC][GT]AC
AcsI         1191  GAATTC               [GA]^AATT[CT]
AcyI          201  GGCGCC               G[GA]^CG[CT]C
AgeI          728  ACCGGT               A^CCGGT
AhdI         1027  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         2005  GATCC                GGATC(4/5)
ApaI         1142  GGGCCC               GGGCC^C
ApoI         1191  GAATTC               [GA]^AATT[CT]
AseI          357  ATTAAT               AT^TAAT
AsnI          357  ATTAAT               AT^TAAT
AspEI        1027  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        1142  GGGCCC               G[GA]GC[CT]^C
BbeI          201  GGCGCC               GGCGC^C
BglI          204  GCCAGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI         1142  GGGCCC               G[AGT]GC[ACT]^C
BsaAI         154  TACGTG               [CT]AC^GT[GA]
BsaHI         201  GGCGCC               G[GA]^CG[CT]C
BsaI          586  GAGACC               GGTCTC(1/5)
BsaWI         728  ACCGGT               [AT]^CCGG[AT]
BsmBI         124  GAGACG               CGTCTC(1/5)
Bsp1286I     1142  GGGCCC               G[AGT]GC[ACT]^C
BsrBI        1250  CCGCTC               CCGCTC(-3/-3)
BsrDI        1330  GCAATG               GCAATG(2/0)
BstYI        2004  AGATCC               [GA]^GATC[CT]
DraI         1089  TTTAAA               TTT^AAA
DrdI          376  GACAACAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          128  CGGCCA               [CT]^GGCC[GA]
Eam1105I     1027  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         964  CCTGGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1191  GAATTC               G^AATTC
EcoT14I       221  CCAAGG               C^C[AT][AT]GG
EcoT22I      1601  ATGCAT               ATGCA^T
HaeII         201  GGCGCC               [GA]GCGC^[CT]
Hin1I         201  GGCGCC               G[GA]^CG[CT]C
HindIII      1711  AAGCTT               A^AGCTT
HpaI         1590  GTTAAC               GTT^AAC
HphI         1173  GGTGA                GGTGA(8/7)
KasI          201  GGCGCC               G^GCGCC
MflI         2004  AGATCC               [GA]^GATC[CT]
NarI          201  GGCGCC               GG^CGCC
NdeI         1108  CATATG               CA^TATG
NsiI         1601  ATGCAT               ATGCA^T
PflMI        1656  CCAGTTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         728  ACCGGT               A^CCGGT
PpuMI          47  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI         357  ATTAAT               AT^TAAT
PstI          214  CTGCAG               CTGCA^G
SapI          844  GAAGAGC              GCTCTTC(1/4)
ScaI         1440  AGTACT               AGT^ACT
SexAI        1628  ACCTGGT              A^CC[AT]GGT
SfoI          201  GGCGCC               GGC^GCC
StuI           77  AGGCCT               AGG^CCT
StyI          221  CCAAGG               C^C[AT][AT]GG
TaqI          561  TCGA                 T^CGA
TthHB8I       561  TCGA                 T^CGA
Van91I       1656  CCAGTTAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          675  CCAGCCCTTTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        2004  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Acc65I        178  GGTACC               G^GTACC
             1632  GGTACC              
AccII         165  CGCG                 CG^CG
              209  CGCG                
AlwNI         254  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              411  CAGTGGCTG           
Asp718I       178  GGTACC               G^GTACC
             1632  GGTACC              
AvaII          48  GGTCC                G^G[AT]CC
              750  GGTCC               
BfaI         1006  CTAG                 C^TAG
             1451  CTAG                
BsmAI         124  GAGAC                GTCTC(1/5)
              586  GAGAC               
BspMI         181  ACCTGC               ACCTGC(4/8)
              747  GCAGGT              
BsrFI         137  ACCGGC               [GA]^CCGG[CT]
              728  ACCGGT              
BstUI         165  CGCG                 CG^CG
              209  CGCG                
Cfr10I        137  ACCGGC               [GA]^CCGG[CT]
              728  ACCGGT              
DpnII         755  GATC                 ^GATC
             2005  GATC                
DraII          47  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1142  GGGCCCC             
EcoO109I       47  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1142  GGGCCCC             
HapII         138  CCGG                 C^CGG
              729  CCGG                
HpaII         138  CCGG                 C^CGG
              729  CCGG                
KpnI          178  GGTACC               GGTAC^C
             1632  GGTACC              
MaeI         1006  CTAG                 C^TAG
             1451  CTAG                
MboI          755  GATC                 ^GATC
             2005  GATC                
MslI          169  CACCTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1699  CACCTTCATG          
MspA1I        554  CAGCTG               C[AC]G^C[GT]G
             1639  CAGCTG              
MspI          138  CCGG                 C^CGG
              729  CCGG                
MvnI          165  CGCG                 CG^CG
              209  CGCG                
NdeII         755  GATC                 ^GATC
             2005  GATC                
NspI          469  ACATGC               [GA]CATG^[CT]
              618  ACATGC              
PvuII         554  CAGCTG               CAG^CTG
             1639  CAGCTG              
Sau3AI        755  GATC                 ^GATC
             2005  GATC                
Tsp45I        107  GTCAC                ^GT[GC]AC
              636  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AfaI        AluI        BanI        BbsI        
BbvI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        EarI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HincII      HindII      HinfI       ItaI        Ksp632I     
MaeII       MaeIII      MboII       MnlI        MseI        
MvaI        MwoI        NlaIII      NlaIV       PleI        
RsaI        Sau96I      ScrFI       SfaNI       SfcI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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