Restriction map for
pF1KB8017
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BbeI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
DrdI EcoRV EcoT22I FspI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 642 GAATTC [GA]^AATT[CT]
AflIII 337 ACGTGT A^C[GA][CT]GT
AhdI 530 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1514 AGCGCT AGC^GCT
ApoI 642 GAATTC [GA]^AATT[CT]
AspEI 530 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 102 TGGCCA TGG^CCA
BamHI 612 GGATCC G^GATCC
BbrPI 336 CACGTG CAC^GTG
BbsI 1464 GAAGAC GAAGAC(2/6)
BclI 1559 TGATCA T^GATCA
BglI 560 GCCCACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1464 GAAGAC GAAGAC(2/6)
BsaAI 336 CACGTG [CT]AC^GT[GA]
BsmBI 1447 CGTCTC CGTCTC(1/5)
BsrBI 1908 GAGCGG CCGCTC(-3/-3)
BstEII 426 GGTGACC G^GT[ACGT]ACC
BstPI 426 GGTGACC G^GT[ACGT]ACC
BstXI 275 CCAAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1070 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 530 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1514 AGCGCT AGC^GCT
EcoNI 1143 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 426 GGTGACC G^GT[ACGT]ACC
EcoRI 642 GAATTC G^AATTC
FbaI 1559 TGATCA T^GATCA
FseI 1833 GGCCGGCC GGCCGG^CC
HincII 6 GTTGAC GT[CT]^[GA]AC
HindII 6 GTTGAC GT[CT]^[GA]AC
MluNI 102 TGGCCA TGG^CCA
MscI 102 TGGCCA TGG^CCA
NspI 1823 GCATGC [GA]CATG^[CT]
PaeR7I 760 CTCGAG C^TCGAG
PmaCI 336 CACGTG CAC^GTG
PmlI 336 CACGTG CAC^GTG
SacI 819 GAGCTC GAGCT^C
SapI 207 GCTCTTC GCTCTTC(1/4)
ScaI 185 AGTACT AGT^ACT
SphI 1823 GCATGC GCATG^C
Tsp509I 643 AATT ^AATT
XhoI 760 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 1474 CCTGAGG CC^T[ACGT]AGG
1927 CCTGAGG
ApaI 230 GGGCCC GGGCC^C
1851 GGGCCC
BanI 574 GGCACC G^G[CT][GA]CC
1548 GGCACC
BsaWI 662 ACCGGA [AT]^CCGG[AT]
1112 TCCGGT
BseRI 882 GAGGAG GAGGAG(10/8)
1123 GAGGAG
BsiEI 1003 CGGCCG CG[GA][CT]^CG
1566 CGGCCG
BstAPI 444 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1037 GCAAGGGCTGC
Bsu36I 1474 CCTGAGG CC^T[ACGT]AGG
1927 CCTGAGG
EagI 1003 CGGCCG C^GGCCG
1566 CGGCCG
EarI 208 CTCTTC CTCTTC(1/4)
1776 GAAGAG
EclXI 1003 CGGCCG C^GGCCG
1566 CGGCCG
Eco52I 1003 CGGCCG C^GGCCG
1566 CGGCCG
Eco81I 1474 CCTGAGG CC^T[ACGT]AGG
1927 CCTGAGG
HaeII 910 GGCGCT [GA]GCGC^[CT]
1514 AGCGCT
Ksp632I 208 CTCTTC CTCTTC(1/4)
1776 GAAGAG
NaeI 983 GCCGGC GCC^GGC
1834 GCCGGC
NgoMIV 983 GCCGGC G^CCGGC
1834 GCCGGC
PshAI 429 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
949 GACCTGGGTC
PstI 162 CTGCAG CTGCA^G
888 CTGCAG
SfcI 162 CTGCAG C^T[GA][CT]AG
888 CTGCAG
TfiI 726 GATTC G^A[AT]TC
1694 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII